LOCUS       pcDNA3.1/Puro-CAG-JEDI-2P-Kv        7610 bp    DNA     circular UNK 30-NOV-2021
DEFINITION  .
ACCESSION   <unknown (modified)
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             join(complement(1..676),complement(7427..7610))
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="AmpR"
     promoter        complement(678..781)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="AmpR promoter"
     enhancer        814..1192
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
                     /label="CMV enhancer"
     promoter        1195..1469
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.281"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="chicken beta-actin promoter"
     intron          1471..2486
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.902"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
                     /label="chimeric intron"
     CDS             2653..4167
                     /created_by="User"
                     /modified_by="User"
                     /label="JEDI-2P-Kv"
     polyA_signal    4285..4508
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA signal"
                     /label="bGH poly(A) signal"
     promoter        4513..4533
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="6.197183098591549"
                     /fragment="True"
                     /other="promoter"
                     /label="SV40 promoter"
     rep_origin      4555..4982
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="f1 ori"
     promoter        4997..5325
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="SV40 promoter"
     CDS             5337..5935
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="PuroR"
     polyA_signal    complement(6096..6225)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="98.496"
                     /match_length="97.08029197080292"
                     /fragment="False"
                     /other="polyA signal"
                     /label="SV40 poly(A) signal"
     protein_bind    6288..6309
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
                     /label="lac operator"
     promoter        complement(6314..6343)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="lac promoter"
     protein_bind    6359..6379
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="CAP binding site"
     CDS             complement(6393..6485)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
                     /label="lacI"
     rep_origin      complement(6668..7255)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
ORIGIN      
        1 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
       61 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      121 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      181 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      241 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      301 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      361 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      421 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
      481 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      541 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      601 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      661 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      721 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
      781 cgcacatttc cccgaaaagt gccacctgac gtcgacattg attattgact agttattaat
      841 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      901 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      961 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     1021 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     1081 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     1141 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
     1201 tgagccccac gttctgcttc actctcccca tctccccccc tccccacccc caattttgta
     1261 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg ggggcgcgcg
     1321 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca
     1381 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
     1441 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg
     1501 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
     1561 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
     1621 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg
     1681 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc
     1741 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1801 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc
     1861 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc
     1921 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg
     1981 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg
     2041 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg
     2101 gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
     2161 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
     2221 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
     2281 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
     2341 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
     2401 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
     2461 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     2521 catcattttg gcaaagaatt cctcgaggaa ttcactcctc aggtgcaggc tgcctatcag
     2581 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctgcatgag
     2641 ctagccgcca ccatggagac gactgtgagg tatgaacagg ggtcagagct cactaaaact
     2701 tcgagctctc caacagcaga tgagcccacg ataaagattg atgatggtcg tgatgagggt
     2761 aatgaacaag acagctgttc caataccatt aggagaaaaa tttccccgtt tgtgatgtca
     2821 tttggattca gagtatttgg agttgtgctt atcattgtag acatcatagt ggtgattgtg
     2881 gatctggcca tcagtgagaa gaaaagaggc attagagaga ttcttgaagg tgtttccctg
     2941 gctatagcac tcttcttcct tgttgatgtt ctcatgagag tgtttgttga aggcttcaag
     3001 aactatttcc ggtccaaact ggttactttg gatgcagtca tagtagtggg cactctgcta
     3061 attaatatga cctactcctt ctctgacctt gctgccttta acagcgagaa cgtgtatatt
     3121 accgcggata aacagaaaaa cggcattaaa gcgaacttta ccgtgcgcca taacgtggaa
     3181 gatggcagcg tgcagctggc ggatcattat cagcagaaca ccccgattgg cgatggcccg
     3241 gtgctgctgc cggataacca ttatctgagc caccagaccg tgctgagcaa agatccgaac
     3301 gaaaaacgcg atcacatggt gctgctggaa tttgtgaccg cagcgggcat tacacacggc
     3361 atggatgaac tgtatggcgg caccggcggc agcgcgagcc agggcgaaga actgtttacc
     3421 ggcgtggtgc cgattctggt ggaactggat ggcgatgtga acggccataa atttagcgtg
     3481 cgcggcgaag gcgaaggcga tgcgaccatt ggcaaactga ccctgaaatt tatttgcacc
     3541 accggcaaac taccggtgcc gtggccgacc ctggtgacca ccttaaccta tggcgtgcag
     3601 tgctttagcc gctatccgga tcatatgaaa cgccatgatt tttttaaaag cgcgatgccg
     3661 gaaggctatg tgcaggaacg caccattagc tttaaagatg atggcaaata taaaacccgc
     3721 gcggtggtga aatttgaagg cgataccctg gtgaaccgca ttgaactgaa aggcaccgat
     3781 tttaaagaag atggcaacat tctggggcat aaactggaat atgatacaga tcagatgccg
     3841 cacatggtta ctcttcttcg agttctgaaa attgttatct taataagaat atttcgcctg
     3901 gcttcacaga agaaacaact tgaagtggta accggttctt caggttcatc tggatcttcc
     3961 acgcgtcagt cccagcccat cctcaacacc aaggagatgg ccccgcagag caagcctcca
     4021 gaggagctgg agatgagcag catgcccagc cccgtggccc ctctgcccgc acgcacggag
     4081 ggcgtcatcg acatgcggag catgtccagc attgacagct tcatcagctg tgccacggac
     4141 ttccctgaag ccaccagatt ctaatgaaag cttggtaccg agctcggatc cactagtcca
     4201 gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg
     4261 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4321 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4381 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4441 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     4501 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     4561 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4621 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4681 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4741 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4801 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4861 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4921 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4981 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5041 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5101 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     5161 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5221 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     5281 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgcatga
     5341 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     5401 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     5461 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     5521 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     5581 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     5641 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     5701 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     5761 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     5821 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     5881 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgattcg
     5941 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     6001 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     6061 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     6121 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6181 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     6241 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6301 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6361 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6421 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6481 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6541 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6601 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6661 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6721 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6781 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6841 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6901 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6961 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7021 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7081 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7141 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7201 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7261 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7321 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7381 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7441 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7501 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7561 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
//
