LOCUS       pAAV-CaMKIIa-JEDI-2P-WPRE        6648 bp    DNA     circular UNK 30-NOV-2021
DEFINITION  .
ACCESSION   <unknown (modified)
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      146..733
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
     misc_feature    789..794
                     /Recognition_pattern="A^CATGT"
                     /annotation_group="ACATGT"
                     /label="PciI, PscI"
                     /note="Geneious type: restriction site"
     repeat_region   796..924
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat region"
                     /label="AAV2 ITR (alternate)"
     promoter        939..2231
                     /created_by="User"
                     /modified_by="User"
                     /label="CaMKIIa"
     misc_feature    1073..1085
                     /Recognition_pattern="(7/12)GAACNNNNNNTAC(12/7)"
                     /annotation_group="GAACNNNNNNTAC"
                     /label="PsrI"
                     /note="Geneious type: restriction site"
     misc_feature    1334..1344
                     /Recognition_pattern="CCTNN^NNNAGG"
                     /annotation_group="CCTNNNNNAGG"
                     /label="EcoNI, BstENI, XagI"
                     /note="Geneious type: restriction site"
     misc_feature    1368..1373
                     /Recognition_pattern="AGG^CCT"
                     /annotation_group="AGGCCT"
                     /label="StuI, Eco147I, PceI, SseBI"
                     /note="Geneious type: restriction site"
     misc_feature    1422..1430
                     /Recognition_pattern="GACN^NNGTC"
                     /annotation_group="GACNNNGTC"
                     /label="Tth111I, PflFI, PsyI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(1440..1445)
                     /Recognition_pattern="GAATGC(1/-1)"
                     /annotation_group="GAATGC"
                     /label="BsmI, Mva1269I, PctI"
                     /note="Geneious type: restriction site"
     misc_feature    1552..1557
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HindII, HincII"
                     /note="Geneious type: restriction site"
     misc_feature    complement(1854..1860)
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /annotation_group="GCTCTTC"
                     /label="SapI, BspQI, LguI, PciSI"
                     /note="Geneious type: restriction site"
     misc_feature    1863..1868
                     /Recognition_pattern="T^GATCA"
                     /annotation_group="TGATCA"
                     /label="BclI, FbaI, Ksp22I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2024..2030)
                     /Recognition_pattern="CACCTGC(4/8)"
                     /annotation_group="CACCTGC"
                     /label="AarI, PaqCI"
                     /note="Geneious type: restriction site"
     misc_feature    2088..2094
                     /Recognition_pattern="RG^GWCCY"
                     /annotation_group="RGGWCCY"
                     /label="PpuMI, Psp5II, PspPPI"
                     /note="Geneious type: restriction site"
     misc_feature    2111..2116
                     /Recognition_pattern="TCG^CGA"
                     /annotation_group="TCGCGA"
                     /label="NruI, Bsp68I, BtuMI, RruI"
                     /note="Geneious type: restriction site"
     misc_feature    2240..2245
                     /Recognition_pattern="G^GTACC"
                     /annotation_group="GGTACC"
                     /label="Acc65I, Asp718I"
                     /note="Geneious type: restriction site"
     misc_feature    2240..2245
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    2246..2251
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     CDS             2258..3544
                     /created_by="User"
                     /modified_by="User"
                     /label="JEDI-2P"
     misc_feature    2462..2467
                     /Recognition_pattern="GT^MKAC"
                     /annotation_group="GTMKAC"
                     /label="AccI, FblI, XmiI"
                     /note="Geneious type: restriction site"
     misc_feature    2986..2993
                     /Recognition_pattern="CR^CCGGYG"
                     /annotation_group="CRCCGGYG"
                     /label="SgrAI"
                     /note="Geneious type: restriction site"
     misc_feature    3227..3232
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(3347..3357)
                     /Recognition_pattern="(10/15)ACNNNNGTAYC(12/7)"
                     /annotation_group="ACNNNNGTAYC"
                     /label="BaeI"
                     /note="Geneious type: restriction site"
     misc_feature    3545..3550
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    3546..3569
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="23.58490566037736"
                     /fragment="True"
                     /other="misc feature"
                     /label="MCS"
     misc_feature    3551..3556
                     /Recognition_pattern="GAT^ATC"
                     /annotation_group="GATATC"
                     /label="EcoRV, Eco32I"
                     /note="Geneious type: restriction site"
     misc_feature    3557..3562
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     misc_feature    3570..4157
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
                     /label="WPRE"
     misc_feature    4168..4173
                     /Recognition_pattern="AGC^GCT"
                     /annotation_group="AGCGCT"
                     /label="Eco47III, AfeI, Aor51HI"
                     /note="Geneious type: restriction site"
     misc_feature    4175..4180
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI, PaeR7I, Sfr274I, SlaI"
                     /note="Geneious type: restriction site"
     misc_feature    4181..4186
                     /Recognition_pattern="A^GATCT"
                     /annotation_group="AGATCT"
                     /label="BglII"
                     /note="Geneious type: restriction site"
     polyA_signal    4190..4665
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA signal"
                     /label="hGH poly(A) signal"
     misc_feature    4242..4251
                     /Recognition_pattern="CACNN^NNGTG"
                     /annotation_group="CACNNNNGTG"
                     /label="OliI, AleI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(4253..4264)
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10)"
                     /annotation_group="CAANNNNNGTGG"
                     /label="CspCI"
                     /note="Geneious type: restriction site"
     misc_feature    4471..4477
                     /Recognition_pattern="CCTCAGC(-5/-2)"
                     /annotation_group="CCTCAGC"
                     /label="BbvCI"
                     /note="Geneious type: restriction site"
     misc_feature    4503..4508
                     /Recognition_pattern="ATGCA^T"
                     /annotation_group="ATGCAT"
                     /label="EcoT22I, Mph1103I, NsiI, Zsp2I"
                     /note="Geneious type: restriction site"
     misc_feature    4683..4688
                     /Recognition_pattern="CAC^GTG"
                     /annotation_group="CACGTG"
                     /label="PmaCI, AcvI, BbrPI, Eco72I, PmlI, PspCI"
                     /note="Geneious type: restriction site"
     misc_feature    4689..4695
                     /Recognition_pattern="CG^GWCCG"
                     /annotation_group="CGGWCCG"
                     /label="RsrII, CpoI, CspI, Rsr2I"
                     /note="Geneious type: restriction site"
     repeat_region   4706..4845
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat region"
                     /label="AAV2 ITR"
     misc_feature    4846..4851
                     /Recognition_pattern="GG^CGCC"
                     /annotation_group="GGCGCC"
                     /label="NarI, Mly113I"
                     /note="Geneious type: restriction site"
     misc_feature    4846..4851
                     /Recognition_pattern="GGCGC^C"
                     /annotation_group="GGCGCC"
                     /label="PluTI"
                     /note="Geneious type: restriction site"
     misc_feature    4846..4851
                     /Recognition_pattern="GGC^GCC"
                     /annotation_group="GGCGCC"
                     /label="DinI, EgeI, EheI, SfoI"
                     /note="Geneious type: restriction site"
     misc_feature    4846..4851
                     /Recognition_pattern="G^GCGCC"
                     /annotation_group="GGCGCC"
                     /label="KasI, SspDI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(4851..4901)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="37.410071942446045"
                     /fragment="True"
                     /other="misc feature"
                     /label="bom"
     rep_origin      4921..5375
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="f1 ori"
     misc_feature    5276..5281
                     /Recognition_pattern="TTA^TAA"
                     /annotation_group="TTATAA"
                     /label="PsiI, AanI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5377..5425)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="35.97122302158273"
                     /fragment="True"
                     /other="misc feature"
                     /label="bom"
     misc_feature    5510..5516
                     /Recognition_pattern="T^CCNGGA"
                     /annotation_group="TCCNGGA"
                     /label="PfoI"
                     /note="Geneious type: restriction site"
     CDS             complement(5528..5562)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="19.047619047619047"
                     /fragment="True"
                     /other="CDS"
                     /label="rop"
     promoter        5658..5761
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="AmpR promoter"
     CDS             5763..6622
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="AmpR"
     misc_feature    6066..6071
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     misc_feature    6177..6182
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI, Ple19I"
                     /note="Geneious type: restriction site"
     misc_feature    6324..6329
                     /Recognition_pattern="TGC^GCA"
                     /annotation_group="TGCGCA"
                     /label="Acc16I, FspI, NsbI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
       61 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
      121 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      181 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      241 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      301 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      361 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      421 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      481 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      541 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      601 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      661 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      721 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      781 ttttgctcac atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc
      841 gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg
      901 ccaactccat cactaggggt tcctgcggcc gcacgcgttt aacattatgg ccttaggtca
      961 cttcatctcc atggggttct tcttctgatt ttctagaaaa tgagatgggg gtgcagagag
     1021 cttcctcagt gacctgccca gggtcacatc agaaatgtca gagctagaac ttgaactcag
     1081 attactaatc ttaaattcca tgccttgggg gcatgcaagt acgatataca gaaggagtga
     1141 actcattagg gcagatgacc aatgagttta ggaaagaaga gtccagggca gggtacatct
     1201 acaccacccg cccagccctg ggtgagtcca gccacgttca cctcattata gttgcctctc
     1261 tccagtccta ccttgacggg aagcacaagc agaaactggg acaggagccc caggagacca
     1321 aatcttcatg gtccctctgg gaggatgggt ggggagagct gtggcagagg cctcaggagg
     1381 ggccctgctg ctcagtggtg acagataggg gtgagaaagc agacagagtc attccgtcag
     1441 cattctgggt ctgtttggta cttcttctca cgctaaggtg gcggtgtgat atgcacaatg
     1501 gctaaaaagc agggagagct ggaaagaaac aaggacagag acagaggcca agtcaaccag
     1561 accaattccc agaggaagca aagaaaccat tacagagact acaaggggga agggaaggag
     1621 agatgaatta gcttcccctg taaaccttag aacccagctg ttgccagggc aacggggcaa
     1681 tacctgtctc ttcagaggag atgaagttgc cagggtaact acatcctgtc tttctcaagg
     1741 accatcccag aatgtggcac ccactagccg ttaccatagc aactgcctct ttgccccact
     1801 taatcccatc ccgtctgtta aaagggccct atagttggag gtgggggagg taggaagagc
     1861 gatgatcact tgtggactaa gtttgttcgc atccccttct ccaaccccct cagtacatca
     1921 ccctggggga acagggtcca cttgctcctg ggcccacaca gtcctgcagt attgtgtata
     1981 taaggccagg gcaaagagga gcaggtttta aagtgaaagg caggcaggtg ttggggaggc
     2041 agttaccggg gcaacgggaa cagggcgttt cggaggtggt tgccatgggg acctggatgc
     2101 tgacgaaggc tcgcgaggct gtgagcagcc acagtgccct gctcagaagc cccaagctcg
     2161 tcagtcaagc cggttctccg tttgcactca ggagcacggg caggcgagtg gcccctagtt
     2221 ctgggggcag ctctagagcg gtaccggatc cgccaccatg gagacgactg tgaggtatga
     2281 acaggggtca gagctcacta aaacttcgag ctctccaaca gcagatgagc ccacgataaa
     2341 gattgatgat ggtcgtgatg agggtaatga acaagacagc tgttccaata ccattaggag
     2401 aaaaatttcc ccgtttgtga tgtcatttgg attcagagta tttggagttg tgcttatcat
     2461 tgtagacatc atagtggtga ttgtggatct ggccatcagt gagaagaaaa gaggcattag
     2521 agagattctt gaaggtgttt ccctggctat agcactcttc ttccttgttg atgttctcat
     2581 gagagtgttt gttgaaggct tcaagaacta tttccggtcc aaactggtta ctttggatgc
     2641 agtcatagta gtgggcactc tgctaattaa tatgacctac tccttctctg accttgctgc
     2701 ctttaacagc gagaacgtgt atattaccgc ggataaacag aaaaacggca ttaaagcgaa
     2761 ctttaccgtg cgccataacg tggaagatgg cagcgtgcag ctggcggatc attatcagca
     2821 gaacaccccg attggcgatg gcccggtgct gctgccggat aaccattatc tgagccacca
     2881 gaccgtgctg agcaaagatc cgaacgaaaa acgcgatcac atggtgctgc tggaatttgt
     2941 gaccgcagcg ggcattacac acggcatgga tgaactgtat ggcggcaccg gcggcagcgc
     3001 gagccagggc gaagaactgt ttaccggcgt ggtgccgatt ctggtggaac tggatggcga
     3061 tgtgaacggc cataaattta gcgtgcgcgg cgaaggcgaa ggcgatgcga ccattggcaa
     3121 actgaccctg aaatttattt gcaccaccgg caaactaccg gtgccgtggc cgaccctggt
     3181 gaccacctta acctatggcg tgcagtgctt tagccgctat ccggatcata tgaaacgcca
     3241 tgattttttt aaaagcgcga tgccggaagg ctatgtgcag gaacgcacca ttagctttaa
     3301 agatgatggc aaatataaaa cccgcgcggt ggtgaaattt gaaggcgata ccctggtgaa
     3361 ccgcattgaa ctgaaaggca ccgattttaa agaagatggc aacattctgg ggcataaact
     3421 ggaatatgat acagatcaga tgccgcacat ggttactctt cttcgagttc tgaaaattgt
     3481 tatcttaata agaatatttc gcctggcttc acagaagaaa caacttgaag tggtaacctg
     3541 ataagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     3601 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3661 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3721 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     3781 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     3841 ttccggaact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3901 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3961 gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac
     4021 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4081 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4141 ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc
     4201 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     4261 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     4321 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     4381 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     4441 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     4501 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     4561 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     4621 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta
     4681 accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     4741 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     4801 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     4861 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     4921 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4981 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5041 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5101 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     5161 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5221 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
     5281 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5341 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     5401 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     5461 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5521 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     5581 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5641 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5701 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5761 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5821 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5881 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5941 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6001 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6061 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6121 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6181 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6241 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6301 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6361 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6421 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6481 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6541 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6601 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatat
//
