LOCUS       Exported                8883 bp DNA     circular SYN 29-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Karim Labib Lab
  TITLE     Direct Submission
  JOURNAL   Exported Nov 29, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             24..1748
                     /codon_start=1
                     /label=OsTIR1 codon plus
                     /note="color: #3366ff"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHAADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     misc_feature    1749..1802
                     /label=T2A
                     /note="color: #a6acb3"
     primer_bind     complement(1785..1805)
                     /label=t?C}[ 7
                     /note="color: ; sequence: TTGCTCACCATggggccggggttctcctc"
     CDS             1803..2225
                     /codon_start=1
                     /label=BSR resistance
                     /note="color: #a6acb3"
                     /translation="MKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIIS
                     AVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCR
                     ELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     misc_feature    2285..2871
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 2285 .. 2859 / #a6acb3
                        2: 2860 .. 2862 / #a6acb3 / ATG
                        3: 2863 .. 2871 / #a6acb3"
     primer_bind     2883..2901
                     /label=t?C}[ 8
                     /note="color: ; sequence: ccccggccccATGGTGAGCAAGGGCGAG"
     primer_bind     2883..2901
                     /label=Primer XX2
                     /note="color: ; sequence: 
                     ctgctgaagcaggctggagacgtcgaggagaacccaggacccATGGTGAGCAAGGGCGA
                     G"
     CDS             2884..3600
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2884 .. 2886 / #05fd14
                        2: 2887 .. 2889 / #05fd14 / 1a
                        3: 2890 .. 3600 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     2884..2901
                     /label=Primer 7
                     /note="color: ; sequence: 
                     ATTAGGCGGATCTGGCGGGatggtgagcaagggcgag"
     primer_bind     3450..3469
                     /label=GFP_F
                     /note="color: ; sequence: CGGCGACGGCCCCGTGCTGC"
     primer_bind     3450..3469
                     /label=petAID2green-Ncheck1
                     /note="color: ; sequence: CGGCGACGGCCCCGTGCTGC"
     primer_bind     complement(3581..3611)
                     /label=Primer 3
                     /note="color: ; sequence: CTCTTCTCCTTCTTGTACAGCTCGTCCATGC"
     primer_bind     complement(3582..3600)
                     /label=Primer 3
                     /note="color: ; sequence: cttgtacagctcgtccatg"
     primer_bind     complement(3582..3600)
                     /label=PrimerXX
                     /note="color: ; sequence: 
                     tctccagcctgcttcagcagggagaagttggtagctccggatcccttgtacagctcgtc
                     catg"
     primer_bind     complement(3582..3600)
                     /label=t?C}[ 9
                     /note="color: ; sequence: 
                     gttaacGATATCTGAaccCCCTCCCTTGTACAGCTCGTCCATG"
     CDS             3601..3804
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /note="color: #cc99b2"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             3805..3834
                     /codon_start=1
                     /label=5GA
                     /note="color: #993366"
                     /translation="GAGAGAGAGA"
     CDS             3838..5349
                     /codon_start=1
                     /label=hLEMD2
                     /note="color: #993366"
                     /translation="MAGLSDLELRRELQALGFQPGPITDTTRDVYRNKLRRLRGEARLR
                     DEERLREEARPRGEERLREEARLREDAPLRARPAAASPRAEPWLSQPASGSAYATPGAY
                     GDIRPSAASWVGSRGLAYPARPAQLRRRASVRGSSEEDEDARTPDRATQGPGLAARRWW
                     AASPAPARLPSSLLGPDPRPGLRATRAGPAGAARARPEVGRRLERWLSRLLLWASLGLL
                     LVFLGILWVKMGKPSAPQEAEDNMKLLPVDCERKTDEFCQAKQKAALLELLHELYNFLA
                     IQAGNFECGNPENLKSKCIPVMEAQEYIANVTSSSSAKFEAALTWILSSNKDVGIWLKG
                     EDQSELVTTVDKVVCLESAHPRMGVGCRLSRALLTAVTNVLIFFWCLAFLWGLLILLKY
                     RWRKLEEEEQAMYEMVKKIIDVVQDHYVDWEQDMERYPYVGILHVRDSLIPPQSRRRMK
                     RVWDRAVEFLASNESRIQTESHRVAGEDMLVWRWTKPSSFSDSER"
     polyA_signal    5367..5488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(5642..6230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    6401..6423
                     /label=Target sequence of pAID-Ef1a linearizing in pX330
                     /note="Off-Targets{0: 1, 1: 0, 2: 0, 3: 6, 4: 58}"
                     /note="color: #a6acb3"
     CDS             complement(6457..7317)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6457 .. 7248 / #ccffcc
                        2: 7249 .. 7317 / #ccffcc / signal sequence
                     Cleavage site after base 7248"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    7422..7470
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    7484..7575
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
                     /note="color: #a6acb3"
     promoter        7675..8856
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    7903..7909
                     /label=5'splice site
                     /note="color: #a6acb3"
     intron          7905..8847
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     protein_bind    8279..8288
                     /label=Sp1
                     /bound_moiety="CGGGCGGCGA"
                     /note="color: #31849b"
     protein_bind    8322..8330
                     /label=Sp1
                     /bound_moiety="GAGGCGGGG"
                     /note="color: #31849b"
     protein_bind    8418..8427
                     /label=Sp1
                     /bound_moiety="CGCCCCGCCC"
                     /note="color: #31849b"
     protein_bind    8428..8437
                     /label=Sp1
                     /bound_moiety="TGGGCGGCAA"
                     /note="color: #31849b"
     protein_bind    8536..8545
                     /label=Sp1
                     /bound_moiety="CGGGCGGGTG"
                     /note="color: #31849b"
     misc_feature    8809..8814
                     /label=splice branch site
                     /note="color: #a6acb3"
     misc_feature    8844..8849
                     /label=3'splice site
                     /note="color: #a6acb3"
ORIGIN
        1 tcttgtccag aggcgctgcc accatgacat actttcctga agaggtcgtc gaacacattt
       61 ttagcttcct gcctgcacag agagatagaa acacagtgag cctggtctgc aaagtgtggt
      121 acgagatcga acgcctgagc cggagaggag tgttcgtcgg caactgctat gctgtgagag
      181 caggcagggt cgccgctagg tttccaaatg tgcgcgcact gaccgtcaag gggaaacccc
      241 acgccgccga ctttaacctg gtgccccctg attggggagg atacgccggc ccttggatcg
      301 aggcagccgc tcgcggctgt catggactgg aggaactgcg catgaagcga atggtggtct
      361 ctgacgaaag tctggagctg ctggctcgga gcttccctag gtttcgcgca ctggtgctga
      421 tttcttgcga aggcttcagc accgatggac tggcagccgt ggcctcccac tgtaagctgc
      481 tgcgggagct ggacctccag gagaatgaag tggaggatag aggccccaga tggctgtctt
      541 gcttcccaga ctcatgtacc agcctggtgt ccctgaactt tgcctgcatc aaaggcgaag
      601 tgaatgctgg gtccctggag cggctggtct caagaagccc caacctgagg tctctgcggc
      661 tgaaccggag cgtgagcgtg gacactctgg ctaagattct gctgagaacc cctaacctgg
      721 aggatctggg aaccggcaat ctgacagacg atttccagac agaatcctac tttaaactga
      781 cttctgccct ggagaagtgt aaaatgctga ggagtctgtc aggattctgg gatgcttcac
      841 ccgtgtgcct gagctttatc taccctctgt gtgcacagct gacaggcctg aacctgagct
      901 atgcaccaac cctggacgcc agtgatctga caaagatgat ctcacgctgc gtgaaactcc
      961 agcgactgtg ggtgctggac tgtatttccg ataaggggct ccaggtggtc gccagctcct
     1021 gcaaggacct ccaggagctg agagtgttcc catctgattt ttacgtggcc ggatatagtg
     1081 ctgtcactga ggaaggcctg gtggcagtct cactgggatg cccaaagctg aacagcctgc
     1141 tgtatttctg tcatcagatg actaatgctg cactggtgac cgtcgccaag aactgcccta
     1201 atttcacccg atttcggctg tgtattctgg aaccaggcaa acccgacgtg gtcacatccc
     1261 agccactgga tgaagggttt ggagctatcg tgagagagtg caagggactc cagaggctga
     1321 gcatttccgg cctgctgaca gacaaagtgt tcatgtacat cggcaagtat gctaagcagc
     1381 tggagatgct gagcattgca tttgccggag actccgataa gggcatgatg cacgtgatga
     1441 acgggtgtaa gaatctgcga aaactggaaa tccgggacag ccctttcggg gatgccgctc
     1501 tgctgggaaa ctttgccaga tacgagacaa tgaggagcct gtggatgtct agttgcaatg
     1561 tgactctgaa gggctgtcag gtcctggcta gtaaaatgcc tatgctgaac gtggaagtca
     1621 ttaatgagcg ggacgggtct aacgaaatgg aggaaaatca tggcgacctg ccaaaggtgg
     1681 agaaactgta tgtgtatcgg accaccgcag gggcaagaga tgatgctccc aactttgtga
     1741 agattctgga gggccgcggc agcctgctga cctgcggcga cgtggaggag aaccccggcc
     1801 ccatgaaaac atttaacatt tctcaacaag atctagaatt agtagaagta gcgacagaga
     1861 agattacaat gctttatgag gataataaac atcatgtggg agcggcaatt cgtacgaaaa
     1921 caggagaaat catttcggca gtacatattg aagcgtatat aggacgagta actgtttgtg
     1981 cagaagccat tgcgattggt agtgcagttt cgaatggaca aaaggatttt gacacgattg
     2041 tagctgttag acacccttat tctgacgaag tagatagaag tattcgagtg gtaagtcctt
     2101 gtggtatgtg tagggagttg atttcagact atgcaccaga ttgttttgtg ttaatagaaa
     2161 tgaatggcaa gttagtcaaa actacgattg aagaactcat tccactcaaa tatacccgaa
     2221 attaaggatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgaggga
     2281 tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     2341 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     2401 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     2461 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     2521 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     2581 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     2641 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     2701 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     2761 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     2821 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catgtgcgat
     2881 aacatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     2941 gacggcgacg taaacggcca caagttcagc gtccgcggcg agggcgaggg cgatgccacc
     3001 aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3061 accctcgtga ccaccttcgg ctacggcgtg gcctgcttca gccgctaccc cgaccacatg
     3121 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3181 tctttcaagg acgacggtac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3241 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3301 cacaagctgg agtacaactt caacagccac tacgtctata tcacggccga caagcagaag
     3361 aactgcatca aggctaactt caagatccgc cacaacgttg aggacggcag cgtgcagctc
     3421 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3481 cactacctga gccatcagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     3541 gtcctgctgg agttcgtgac cgccgccggg attacacatg gcatggacga gctgtacaag
     3601 aaggagaaga gtgcttgtcc taaagatcca gccaaacctc cggccaaggc acaagttgtg
     3661 ggatggccac cggtgagatc ataccggaag aacgtgatgg tttcctgcca aaaatcaagc
     3721 ggtggcccgg aggcggcggc gttcgtgaag gtatcaatgg acggagcacc gtacttgagg
     3781 aaaatcgatt tgaggatgta taaaggagct ggtgcaggcg ctggagcggg tgccgatatg
     3841 gccggcctgt cggacctgga actgcggcgg gagctgcagg ccctgggctt ccagccagga
     3901 cccatcaccg acaccacccg ggatgtctac cgcaacaagc tgcgccgcct gcggggcgag
     3961 gcccggctgc gcgacgagga gcggctgcgg gaggaggccc ggccgcgggg cgaggagcgg
     4021 ttacgggaag aggcccggtt acgcgaggat gcgccgctgc gcgcccggcc cgccgcggcc
     4081 tctccgcggg cggagccctg gctctcccag ccggcctcgg gctcggccta cgcgacccct
     4141 ggggcctacg gtgatatccg gccctccgcg gcttcctggg tagggagccg cggcctcgcc
     4201 tatcctgccc gcccggcgca actcaggcgc cgcgcctcgg tccggggcag ctccgaggag
     4261 gacgaggacg cccggacgcc cgacagggcc acgcagggcc cgggtctcgc ggcccgccgc
     4321 tggtgggcag cgtctcccgc cccggcgcgg ctgccttcct ccctcctcgg tcccgacccg
     4381 cgcccgggcc tgcgggcgac tcgagcgggc cctgctggcg cggcgagggc ccggcctgag
     4441 gtggggcgcc ggctggagcg ctggctctct cggcttctgc tctgggccag cctagggcta
     4501 ctgctcgtct tcctgggcat cctttgggtg aagatgggca agccctcagc gccgcaggag
     4561 gcggaggaca acatgaagtt attgccagtg gactgtgaga gaaaaacaga tgagttctgt
     4621 caggccaagc agaaggcagc cttgctggag ctgctgcatg aactctacaa tttcctggcc
     4681 atccaagctg gtaattttga gtgtggaaat ccagagaatc taaaaagcaa atgcattcct
     4741 gttatggaag cccaagaata tatagccaat gtgaccagca gctcctccgc caagtttgaa
     4801 gccgcactga cctggatact gagcagtaac aaggacgtgg gcatctggtt gaaaggagaa
     4861 gaccagtctg aattggtgac gactgtggac aaggtggtct gcctggaatc tgcccacccc
     4921 cgcatgggtg ttggctgccg cctgagccgg gccttgctca ctgctgtcac caacgtgctc
     4981 atcttcttct ggtgcttggc ttttttgtgg gggctcctaa ttctcctaaa atatcggtgg
     5041 cgaaagttag aagaggagga acaagccatg tatgagatgg tgaagaagat tatagacgtg
     5101 gtccaggacc attacgtgga ctgggagcag gacatggagc gctatccata tgtaggcatc
     5161 ctgcacgtgc gcgacagctt gatccctcca cagagccgga ggcgcatgaa gcgtgtctgg
     5221 gaccgagctg tggagttcct ggcctccaac gaatcccgga tccagacgga gtcccaccgc
     5281 gttgcaggag aggacatgct ggtgtggaga tggactaagc cctcttcctt ctctgactca
     5341 gagcgataaa tccaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     5401 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5461 gtttgtccaa actcatcaat gtatcttagc gctcggtcgt tcggctgcgg cgagcggtat
     5521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6061 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6361 aatcaatcta aagtatatat gagtaaactt ggtctgacag cccctgcgca acgtggcagg
     6421 aagcgcgcgc tggcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc
     6481 accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta
     6541 gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga
     6601 gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg
     6661 aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc
     6721 tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat
     6781 cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag
     6841 ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat
     6901 cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa
     6961 ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa
     7021 gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga
     7081 caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg
     7141 gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc
     7201 acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg
     7261 caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact
     7321 cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata
     7381 agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt
     7441 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     7501 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     7561 ccagaacatt tctctggcct aacnddtsaa gctttgcaaa gatggataaa gttttaaaca
     7621 gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtgcg tgaggctccg
     7681 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     7741 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     7801 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     7861 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     7921 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     7981 cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     8041 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     8101 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     8161 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     8221 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     8281 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     8341 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     8401 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     8461 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     8521 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     8581 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     8641 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     8701 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     8761 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     8821 gttcaaagtt tttttcttcc atttcaggtg tcgtgaattg cggaaggccg tcaaggccac
     8881 gtg
//
