LOCUS       Exported                9065 bp DNA     circular SYN 29-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 29, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
COMMENT     Ligation of : ePB-Puro-TT-MagCre_1.0.xdna [9009  nt] : 
            (PspXI-Blunt(filled)[1051] / PspXI-Blunt(filled)[1051]) to 
            PspXI_T2A.xdna [56  nt] : (Uncut 5'[0] / Uncut 3'[56])
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..341
                     /label=TRE
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    360..362
                     /label=START
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    366..488
                     /label=CREsplit_1
                     /note="color: #800080; direction: RIGHT"
     misc_feature    495..944
                     /label=nMAG
                     /note="color: #0000a0; direction: RIGHT"
     misc_feature    963..986
                     /label=SV40 NLS1
                     /note="color: #008000; direction: RIGHT"
     misc_feature    990..1055
                     /label=P2A
                     /note="color: #0080c0; direction: RIGHT"
     misc_feature    1062..1115
                     /label=T2A
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    1125..1148
                     /label=SV40 NLS2
                     /note="color: #008000; direction: RIGHT"
     misc_feature    1152..1601
                     /label=pMAG
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    1607..2459
                     /label=CREsplit_2
                     /note="color: #8080ff; direction: RIGHT"
     misc_feature    2460..2462
                     /label=STOP
                     /note="color: #804040; direction: RIGHT"
     misc_feature    2477..2713
                     /label=SV40 late pA
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    2720..2963
                     /label=3TR
                     /note="color: #ffff00; direction: RIGHT"
     primer_bind     complement(2994..3013)
                     /label=T7
                     /note="color: #008b8b; direction: LEFT"
     primer_bind     complement(3023..3040)
                     /label=M13-fwd
                     /note="color: #008b8b; direction: LEFT"
     CDS             3111..3179
                     /label=LacZ alpha
                     /note="color: #6495ed; direction: RIGHT"
     rep_origin      complement(3186..3626)
                     /direction=LEFT
                     /label=F1 ori
                     /note="color: #4682b4"
     promoter        3697..3725
                     /label=Amp prom
                     /note="color: #4682b4; direction: RIGHT"
     CDS             3965..4624
                     /label=AmpR
                     /note="color: #8fbc8f; direction: RIGHT"
     rep_origin      4776..5404
                     /direction=RIGHT
                     /label=ColE1 origin
                     /note="color: #4682b4"
     promoter        5710..5739
                     /label=lac
                     /note="color: #4682b4; direction: RIGHT"
     misc_binding    5744..5766
                     /label=LacO
                     /note="color: #6495ed; direction: RIGHT"
     primer_bind     5772..5792
                     /label=M13-rev
                     /note="color: #008b8b; direction: RIGHT"
     primer_bind     5810..5829
                     /label=T3
                     /note="color: #008b8b; direction: RIGHT"
     misc_feature    complement(5862..6102)
                     /label=5TR
                     /note="color: #ffff00; direction: LEFT"
     misc_feature    complement(6188..6318)
                     /label=earlySV40pA
                     /note="color: #84a4c0; direction: LEFT"
     misc_feature    complement(6365..6964)
                     /label=PURO
                     /note="color: #ff8040; direction: LEFT"
     misc_feature    complement(6968..7021)
                     /label=T2A
                     /note="color: #80ff00; direction: LEFT"
     misc_feature    complement(7022..7765)
                     /label=rtTA-Advanced
                     /note="color: #84a4c0; direction: LEFT"
     promoter        complement(7768..7834)
                     /label=EM7 promoter
                     /note="color: #6495ed; direction: LEFT"
     misc_feature    complement(7842..9056)
                     /label=hUBCp
                     /note="color: #808040; direction: LEFT"
ORIGIN
        1 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
       61 gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc
      121 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      181 gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      241 gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
      301 gaaccgtcag atcgcctgga gaattcgagc tcggtacccg gggatccaag cttaccacca
      361 tggccacctc tgatgaagtc aggaagaacc tgatggacat gttcagggac aggcaggcct
      421 tctctgaaca cacctggaag atgctcctgt ctgtgtgcag atcctgggct gcctggtgca
      481 agctgaacgg tacccatact ctttatgccc ccggtggata tgacattatg ggatatctgg
      541 accagatcgg caaccggcca aacccgcagg tggaactggg ccccgtggat acatcctgcg
      601 ccttgattct ttgtgacctg aaacagaaag acaccccgat agtttacgcg agtgaagcct
      661 tcctctacat gacaggttac agcaacgcag aggtgctggg ccggaattgc cggtttctgc
      721 aaagccctga cggcatggtg aagcccaaga gcacccggaa gtacgtggat agtaacacaa
      781 tcaatactat gcgcaaggca atcgacagga atgccgaggt gcaggttgaa gtagtcaatt
      841 ttaaaaagaa tggacagcga tttgttaatt tcctgactat gatacctgtt agggacgaaa
      901 caggcgagta tcgatactct atgggattcc agtgcgaaac agaaggcgga agcggtggcg
      961 tgcccaagaa gaagaggaaa gtcggatccg gcagcggcgc caccaacttc agcctgctga
     1021 agcaggccgg cgacgtggag gagaaccccg gccccctcga cgagggcaga ggaagtcttc
     1081 taacatgcgg tgacgtggag gagaatcccg gccctgtcga ggtgcccaag aagaagagga
     1141 aagtcggcgg acatactctt tatgcccccg gtggatatga cattatggga tatctgaggc
     1201 agatcaggaa ccggccaaac ccgcaggtgg aactgggccc cgtggataca tcctgcgcct
     1261 tgattctttg tgacctgaaa cagaaagaca ccccgatagt ttacgcgagt gaagccttcc
     1321 tctacatgac aggttacagc aacgcagagg tgctgggccg gaattgccgg tttctgcaaa
     1381 gccctgacgg catggtgaag cccaagagca cccggaagta cgtggatagt aacacaatca
     1441 atactatgcg caaggcaatc gacaggaatg ccgaggtgca ggttgaagta gtcaatttta
     1501 aaaagaatgg acagcgattt gttaatttcc tgactatgat acctgttagg gacgaaacag
     1561 gcgagtatcg atactctatg ggattccagt gcgaaacaga aggtaccaac aggaaatggt
     1621 tccctgctga acctgaggat gtgagggact acctcctgta cctgcaagcc agaggcctgg
     1681 ctgtgaagac catccaacag cacctgggcc agctcaacat gctgcacagg agatctggcc
     1741 tgcctcgccc ttctgactcc aatgctgtgt ccctggtgat gaggagaatc agaaaggaga
     1801 atgtggatgc tggggagaga gccaagcagg ccctggcctt tgaacgcact gactttgacc
     1861 aagtcagatc cctgatggag aactctgaca gatgccagga catcaggaac ctggccttcc
     1921 tgggcattgc ctacaacacc ctgctgcgca ttgccgaaat tgccagaatc agagtgaagg
     1981 acatctcccg caccgatggt gggagaatgc tgatccacat tggcaggacc aagaccctgg
     2041 tgtccacagc tggtgtggag aaggccctgt ccctgggggt taccaagctg gtggagagat
     2101 ggatctctgt gtctggtgtg gctgatgacc ccaacaacta cctgttctgc cgggtcagaa
     2161 agaatggtgt ggctgcccct tctgccacct cccaactgtc cacccgggcc ctggaaggga
     2221 tctttgaggc cacccaccgc ctgatctatg gtgccaagga tgactctggg cagagatacc
     2281 tggcctggtc tggccactct gccagagtgg gtgctgccag ggacatggcc agggctggtg
     2341 tgtccatccc tgaaatcatg caggctggtg gctggaccaa tgtgaacatt gtgatgaact
     2401 acatcagaaa cctggactct gagactgggg ccatggtgag gctgctcgaa gatggggact
     2461 aatctagagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     2521 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     2581 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2641 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2701 aatgtatctt aaggaattcg ataaaagttt tgttacttta tagaagaaat tttgagtttt
     2761 tgtttttttt taataaataa ataaacataa ataaattgtt tgttgaattt attattagta
     2821 tgtaagtgta aatataataa aacttaatat ctattcaaat taataaataa acctcgatat
     2881 acagaccgat aaaacacatg cgtcaatttt acgcatgatt atctttaacg tacgtcacaa
     2941 tatgattatc tttctagggt taatctagct gcggcgcgcc ggtacccaat tcgccctata
     3001 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     3061 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     3121 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     3181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     3421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     3481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     3541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3601 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     3661 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3721 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3781 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3841 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3901 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3961 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4021 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4081 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4141 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4201 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4261 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4321 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4381 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4441 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4501 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4561 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4621 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4681 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4741 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4801 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4861 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4921 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4981 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5041 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5101 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5161 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5221 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5281 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5341 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5401 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5461 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5521 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     5581 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     5641 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     5701 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     5761 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     5821 ctaaagggaa caaaagctgg agctccaccg cggggcgcgc cgcagctaga ttaaccctag
     5881 aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc tctttctaaa
     5941 tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga gctcccgtga
     6001 ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacaacc gcgtgagtca
     6061 aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg ataattatat
     6121 tgttatttcg tgttctactt acgtgataac ttattatata tatattttct tgttatagat
     6181 gggaattcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     6241 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     6301 agctgcaata aacaagtttc gaggtcgagt gtcagtcctg ctcctcggcc acgaagtgct
     6361 tagctcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga
     6421 cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg
     6481 tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg
     6541 cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg
     6601 cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc
     6661 gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga
     6721 cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc cacaccttgc
     6781 cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt
     6841 ggcggtccgg gtcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg
     6901 tgcgtacggc ccgggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg
     6961 ccatggtagg gccgggattc tcctccacgt caccgcatgt tagaagactt cctctgccct
     7021 ccccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc atatcaaggt
     7081 caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca agggcgtcgg
     7141 ccggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt agttccaggc
     7201 cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct tgtctcagaa
     7261 gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact tgatgctcct
     7321 gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg tacagtgcgt
     7381 tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg gtttcgtact
     7441 gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt gagaggagag
     7501 cacagcggaa tgacttggcg ttgttccgca gaaagtcttg ccatgactcg ccttccaggg
     7561 ggcagaagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg agcagggccc
     7621 gcttgttctt cacgtgccag tacagggtag gctgctcaac tcccagcttt tgagcgagtt
     7681 tccttgtcgt caggccttcg ataccgactc cattgagtaa ttccagagcg ccgtttatga
     7741 ctttgctctt gtccagtcta gacatggggt ttagttcctc accttgtcgt attatactat
     7801 gccgatatac tatgccgatg attaattgtc aacacggtcc ggtctaacaa aaaagccaaa
     7861 aacggccaga atttagcgga caatttacta gtctaacact gaaaattaca tattgaccca
     7921 aatgattaca tttcaaaagg tgcctaaaaa acttcacaaa acacactcgc caaccccgag
     7981 cgcatagttc aaaaccggag cttcagctac ttaagaagat aggtacataa aaccgaccaa
     8041 agaaactgac gcctcactta tccctcccct caccagaggt ccggcgcctg tcgattcagg
     8101 agagcctacc ctaggcccga accctgcgtc ctgcgacgga gaaaagccta ccgcacacct
     8161 accggcaggt ggccccaccc tgcattataa gccaacagaa cgggtgacgt cacgacacga
     8221 cgagggcgcg cgctcccaaa ggtacgggtg cactgcccaa cggcaccgcc ataactgccg
     8281 cccccgcaac agacgacaaa ccgagttctc cagtcagtga caaacttcac gtcagggtcc
     8341 ccagatggtg ccccagccca tctcacccga ataagagctt tcccgcatta gcgaaggcct
     8401 caagaccttg ggttcttgcc gcccaccatg ccccccacct tgtttcaacg acctcacagc
     8461 ccgcctcaca agcgtcttcc attcaagact cgggaacagc cgccattttg ctgcgctccc
     8521 cccaaccccc agttcagggc aaccttgctc gcggacccag actacagccc ttggcggtct
     8581 ctccacacgc ttccgtccca ccgagcggcc cggcggccac gaaagccccg gccagcccag
     8641 cagcccgcta ctcaccaagt gacgatcaca gcgatccaca aacaagaacc gcgacccaaa
     8701 tcccggctgc gacggaacta gctgtgccac acccggcgcg tccttatata atcatcggcg
     8761 ttcaccgccc cacggagatc cctccgcaga atcgccgaga agggactact tttcctcgcc
     8821 tgttccgctc tctggaaaga aaaccagtgc cctagagtca cccaagtccc gtcctaaaat
     8881 gtccttctgc tgatactggg gttctaaggc cgagtcttat gagcagcggg ccgctgtcct
     8941 gagcgtccgg gcggaaggat caggacgctc gtgcgccctt cgtctgacgt ggcagcgctc
     9001 gccgtgagga gggggcgccc gcggggggcg ccaaaacccg gcgcggaggc cagatctccc
     9061 gatct
//
