LOCUS       Exported               10729 bp DNA     circular SYN 29-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 29, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
COMMENT     Ligation of : 
            ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-BsmBI-CCCA.txt.xdna 
            [9330  nt] : (NotI[8182] / #BsmBI[8172]) to 
            SHH_pcr_BsmBI-NotI.txt.xdna [1391  nt] : (#BsaI[1] / NotI[1401])
FEATURES             Location/Qualifiers
     source          1..10729
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        59..87
                     /label=Amp prom
                     /note="color: #4682b4; direction: RIGHT"
     CDS             327..986
                     /label=AmpR
                     /note="color: #8fbc8f; direction: RIGHT"
     rep_origin      1138..1766
                     /direction=RIGHT
                     /label=ColE1 origin
                     /note="color: #4682b4"
     promoter        2072..2101
                     /label=lac
                     /note="color: #4682b4; direction: RIGHT"
     misc_binding    2106..2128
                     /label=LacO
                     /note="color: #6495ed; direction: RIGHT"
     primer_bind     2134..2154
                     /label=M13-rev
                     /note="color: #008b8b; direction: RIGHT"
     primer_bind     2172..2191
                     /label=T3
                     /note="color: #008b8b; direction: RIGHT"
     misc_feature    complement(2233..2542)
                     /label=5'TR
                     /note="color: #ffff00; direction: LEFT"
     misc_feature    complement(2550..2680)
                     /label=earlySV40pA
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(2722..3120)
                     /label=BSD
                     /note="color: #0080ff; direction: LEFT"
     promoter        complement(3121..3187)
                     /label=EM7 promoter
                     /note="color: #6495ed; direction: LEFT"
     misc_feature    complement(3195..4409)
                     /label=UBC prom
                     /note="color: #ff00ff; direction: LEFT"
     misc_feature    4422..6227
                     /label=CAG
                     /note="color: #ff80c0; direction: RIGHT"
     misc_recomb     6301..6334
                     /label=LoxP
                     /note="color: #4682b4; direction: RIGHT"
     misc_feature    6369..7046
                     /label=dsRed
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    7063..7299
                     /label=SV40 late polyA
                     /note="color: #00ffff; direction: RIGHT"
     misc_recomb     7329..7362
                     /label=LoxP
                     /note="color: #4682b4; direction: RIGHT"
     misc_feature    7388..7408
                     /label=SV40-NLS
                     /note="color: #84a4c0; direction: RIGHT"
     misc_feature    7412..8116
                     /label=NG
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    8117..8170
                     /label=T2A
                     /note="color: #ff00ff; direction: RIGHT"
     misc_feature    8171..9559
                     /label=hSHH
                     /note="color: #33cccc"
     misc_feature    9568..9804
                     /label=SV40 late polyA
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    9811..10054
                     /label=3'TR
                     /note="color: #ffff00; direction: RIGHT"
     primer_bind     complement(10085..10104)
                     /label=T7
                     /note="color: #008b8b; direction: LEFT"
     primer_bind     complement(10114..10131)
                     /label=M13-fwd
                     /note="color: #008b8b; direction: LEFT"
     CDS             10202..10270
                     /label=LacZ alpha
                     /note="color: #6495ed; direction: RIGHT"
     rep_origin      complement(10277..10717)
                     /direction=LEFT
                     /label=F1 ori
                     /note="color: #4682b4"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggggcgc
     2221 gccgcagcta gattaaccct agaaagatag tctgcgtaaa attgacgcat gcattcttga
     2281 aatattgctc tctctttcta aatagcgcga atccgtcgct gtgcatttag gacatctcag
     2341 tcgccgcttg gagctcccgt gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga
     2401 actataacaa ccgcgtgagt caaaatgacg catgattatc ttttacgtga cttttaagat
     2461 ttaactcata cgataattat attgttattt cgtgttctac ttacgtgata acttattata
     2521 tatatatttt cttgttatag atgggaattc agacatgata agatacattg atgagtttgg
     2581 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2641 tgctttattt gtaaccatta taagctgcaa taaacaagtt tcgaggtcga gtgtcagtcc
     2701 tgctcctcgg ccacgaagtg cttagccctc ccacacataa ccagagggca gcaattcacg
     2761 aatcccaact gccgtcggct gtccatcact gtccttcact atggctttga tcccaggatg
     2821 cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc aagatgcccc tgttctcatt
     2881 tccgatcgcg acgatacaag tcaggttgcc agctgccgca gcagcagcag tgcccagcac
     2941 cacgagttct gcacaaggtc ccccagtaaa atgatataca ttgacaccag tgaagatgcg
     3001 gccgtcgcta gagagagctg cgctggcgac gctgtagtct tcagagatgg ggatgctgtt
     3061 gattgtagcc gttgctcttt caatgagggt ggattcttct tgagacaaag gcttggccat
     3121 ggtttagttc ctcaccttgt cgtattatac tatgccgata tactatgccg atgattaatt
     3181 gtcaacacgg tccggtctaa caaaaaagcc aaaaacggcc agaatttagc ggacaattta
     3241 ctagtctaac actgaaaatt acatattgac ccaaatgatt acatttcaaa aggtgcctaa
     3301 aaaacttcac aaaacacact cgccaacccc gagcgcatag ttcaaaaccg gagcttcagc
     3361 tacttaagaa gataggtaca taaaaccgac caaagaaact gacgcctcac ttatccctcc
     3421 cctcaccaga ggtccggcgc ctgtcgattc aggagagcct accctaggcc cgaaccctgc
     3481 gtcctgcgac ggagaaaagc ctaccgcaca cctaccggca ggtggcccca ccctgcatta
     3541 taagccaaca gaacgggtga cgtcacgaca cgacgagggc gcgcgctccc aaaggtacgg
     3601 gtgcactgcc caacggcacc gccataactg ccgcccccgc aacagacgac aaaccgagtt
     3661 ctccagtcag tgacaaactt cacgtcaggg tccccagatg gtgccccagc ccatctcacc
     3721 cgaataagag ctttcccgca ttagcgaagg cctcaagacc ttgggttctt gccgcccacc
     3781 atgcccccca ccttgtttca acgacctcac agcccgcctc acaagcgtct tccattcaag
     3841 actcgggaac agccgccatt ttgctgcgct ccccccaacc cccagttcag ggcaaccttg
     3901 ctcgcggacc cagactacag cccttggcgg tctctccaca cgcttccgtc ccaccgagcg
     3961 gcccggcggc cacgaaagcc ccggccagcc cagcagcccg ctactcacca agtgacgatc
     4021 acagcgatcc acaaacaaga accgcgaccc aaatcccggc tgcgacggaa ctagctgtgc
     4081 cacacccggc gcgtccttat ataatcatcg gcgttcaccg ccccacggag atccctccgc
     4141 agaatcgccg agaagggact acttttcctc gcctgttccg ctctctggaa agaaaaccag
     4201 tgccctagag tcacccaagt cccgtcctaa aatgtccttc tgctgatact ggggttctaa
     4261 ggccgagtct tatgagcagc gggccgctgt cctgagcgtc cgggcggaag gatcaggacg
     4321 ctcgtgcgcc cttcgtctga cgtggcagcg ctcgccgtga ggagggggcg cccgcggggg
     4381 gcgccaaaac ccggcgcgga ggccagatct cccgatctcg agcgacattg attattgact
     4441 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     4501 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     4561 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     4621 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     4681 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     4741 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     4801 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
     4861 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
     4921 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
     4981 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
     5041 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
     5101 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
     5161 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
     5221 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
     5281 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
     5341 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
     5401 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
     5461 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
     5521 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
     5581 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
     5641 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
     5701 gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
     5761 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
     5821 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
     5881 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     5941 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     6001 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     6061 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     6121 attgtgctgt ctcatcattt tggcaaagaa ttaaatttaa ttaatctcga cggtatcggt
     6181 taacgaatcc aagggcgaat tccagcacac tggcggccgt tactagtgga tcccccgggc
     6241 tgcaggaatt cggcgcgcca cgcgtaccga tagcttcgaa ttctgcagtc gaccggaatt
     6301 ataacttcgt atagcataca ttatacgaag ttataattct gcagatatcc agcacagtgg
     6361 cggccaccat ggatagcact gagaacgtca tcaagccctt catgcgcttc aaggtgcaca
     6421 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggcaagccct
     6481 acgagggcac ccagaccgcc aagctgcagg tgaccaaggg cggccccctg cccttcgcct
     6541 gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag caccccgccg
     6601 acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     6661 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcacct
     6721 tcatctacca cgtgaagttc atcggcgtga acttcccctc cgacggcccc gtaatgcaga
     6781 agaagactct gggctgggag ccctccaccg agcgcctgta cccccgcgac ggcgtgctga
     6841 agggcgagat ccacaaggcg ctgaagctga agggcggcgg ccactacctg gtggagttca
     6901 agtcaatcta catggccaag aagcccgtga agctgcccgg ctactactac gtggactcca
     6961 agctggacat cacctcccac aacgaggact acaccgtggt ggagcagtac gagcgcgccg
     7021 aggcccgcca ccacctgttc cagtagagct agctacaagc ttgactctag atcataatca
     7081 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     7141 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     7201 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7261 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg aattcgctag ctgaataagg
     7321 ccgctcgaat aacttcgtat agcatacatt atacgaagtt attcgagtct agaggaccgg
     7381 tatgggccca aagaagaaga gaaaggtttc ggtgagcaag ggcgaggagg ataacatggc
     7441 ctctctccca gcgacacatg agttacacat ctttggctcc atcaacggtg tggactttga
     7501 catggtgggt cagggcaccg gcaatccaaa tgatggttat gaggagttaa acctgaagtc
     7561 caccaagggt gacctccagt tctccccctg gattctggtc cctcatatcg ggtatggctt
     7621 ccatcagtac ctgccctacc ctgacgggat gtcgcctttc caggccgcca cggtagatgg
     7681 ctccggatac caagtccatc gcacaatgca gtttgaagat ggtgcctccc ttactgttaa
     7741 ctaccgctac acctacgagg gaagccacat caaaggagag gcccaggtga aggggactgg
     7801 tttccctgct gacggtcctg tgatgaccaa ctcgctgacc gctgcggact ggtgcaggtc
     7861 gaagaagact taccccaacg acaaaaccat catcagtacc tttaagtgga gttacaccac
     7921 tggaaatggc aagcgctacc ggagcactgc gcggaccacc tacacctttg ccaagccaat
     7981 ggcggctaac tatctgaaga accagccgat gtacgtgttc cgtaagacgg agctcaagca
     8041 ctccaagacc gagctcaact tcaaggagtg gcaaaaggcc tttaccgatg tgatgggcat
     8101 ggacgagctg tacaaggagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     8161 tcccggccca atgctgctgc tggcgagatg tctgctgcta gtcctcgtct cctcgctgct
     8221 ggtatgctcg ggactggcgt gcggaccggg cagggggttc gggaagagga ggcaccccaa
     8281 aaagctgacc cctttagcct acaagcagtt tatccccaat gtggccgaga agaccctagg
     8341 cgccagcgga aggtatgaag ggaagatctc cagaaactcc gagcgattta aggaactcac
     8401 ccccaattac aaccccgaca tcatatttaa ggatgaagaa aacaccggag cggacaggct
     8461 gatgactcag aggtgtaagg acaagttgaa cgctttggcc atctcggtga tgaaccagtg
     8521 gccaggagtg aaactgcggg tgaccgaggg ctgggacgaa gatggccacc actcagagga
     8581 gtctctgcac tacgagggcc gcgcagtgga catcaccacg tctgaccgcg accgcagcaa
     8641 gtacggcatg ctggcccgcc tggcggtgga ggccggcttc gactgggtgt actacgagtc
     8701 caaggcacat atccactgct cggtgaaagc agagaactcg gtggcggcca aatcgggagg
     8761 ctgcttcccg ggctcggcca cggtgcacct ggagcagggc ggcaccaagc tggtgaagga
     8821 cctgagcccc ggggaccgcg tgctggcggc ggacgaccag ggccggctgc tctacagcga
     8881 cttcctcact ttcctggacc gcgacgacgg cgccaagaag gtcttctacg tgatcgagac
     8941 gcgggagccg cgcgagcgcc tgctgctcac cgccgcgcac ctgctctttg tggcgccgca
     9001 caacgactcg gccaccgggg agcccgaggc gtcctcgggc tcggggccgc cttccggggg
     9061 cgcactgggg cctcgggcgc tgttcgccag ccgcgtgcgc ccgggccagc gcgtgtacgt
     9121 ggtggccgag cgtgacgggg accgccggct cctgcccgcc gctgtgcaca gcgtgaccct
     9181 aagcgaggag gccgcgggcg cctacgcgcc gctcacggcc cagggcacca ttctcatcaa
     9241 ccgggtgctg gcctcgtgct acgcggtcat cgaggagcac agctgggcgc accgggcctt
     9301 cgcgcccttc cgcctggcgc acgcgctcct ggctgcactg gcgcccgcgc gcacggaccg
     9361 cggcggggac agcggcggcg gggaccgcgg gggcggcggc ggcagagtag ccctaaccgc
     9421 tccaggtgct gccgacgctc cgggtgcggg ggccaccgcg ggcatccact ggtactcgca
     9481 gctgctctac caaataggca cctggctcct ggacagcgag gccctgcacc cgctgggcat
     9541 ggcggtcaag tccagctgag cggccgcgac tctagatcat aatcagccat accacatttg
     9601 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     9661 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     9721 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9781 ccaaactcat caatgtatct taaggaattc gataaaagtt ttgttacttt atagaagaaa
     9841 ttttgagttt ttgttttttt ttaataaata aataaacata aataaattgt ttgttgaatt
     9901 tattattagt atgtaagtgt aaatataata aaacttaata tctattcaaa ttaataaata
     9961 aacctcgata tacagaccga taaaacacat gcgtcaattt tacgcatgat tatctttaac
    10021 gtacgtcaca atatgattat ctttctaggg ttaatctagc tgcggcgcgc cggtacccaa
    10081 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
    10141 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
    10201 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
    10261 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
    10321 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
    10381 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
    10441 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
    10501 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
    10561 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
    10621 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
    10681 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttag
//
