LOCUS       pJH3577                 8340 bp    DNA        circular     01-NOV-2021
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          27..2922
                     /organism="Caenorhabditis elegans"
                     /mol_type="genomic DNA"
                     /strain="Bristol N2"
                     /db_xref="taxon:6239"
                     /clone="F33D11"
                     /locus_tag="Pceh-12"
                     /label="Pceh-12"
                     /ApEinfo_label="Pceh-12"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(2926..2930,2933..4143)
                     /locus_tag="Chrimson"
                     /label="Chrimson"
                     /ApEinfo_label="Chrimson"
                     /ApEinfo_fwdcolor="#ff8753"
                     /ApEinfo_revcolor="#6373ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2926..2949
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             4144..4144
                     /vntifkey="4"
                     /locus_tag="Chop2(H134R)"
                     /label="Chop2(H134R)"
                     /ApEinfo_label="Chop2(H134R)"
                     /ApEinfo_fwdcolor="#f7ff5d"
                     /ApEinfo_revcolor="#ffaadc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(4119..4143)
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             4154..4861
                     /vntifkey="4"
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#ff5d60"
                     /ApEinfo_revcolor="#ffaadc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     4437..4458
                     /vntifkey="28"
                     /locus_tag="RP_oSR788"
                     /label="RP_oSR788"
                     /ApEinfo_label="RP_oSR788"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      7145..7827
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     4512..4535
                     /vntifkey="28"
                     /locus_tag="FP\oSR780"
                     /label="FP\oSR780"
                     /ApEinfo_label="FP\oSR780"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     8195..8215
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             6388..7047
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    8167..8189
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="cornflower blue"
                     /ApEinfo_revcolor="cornflower blue"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 AAGCTTGGGC TGCAgcatgc ggccgcttta gcgtatagta ttcggataga aatgtgtaaa
       61 aatgatgaaa agcgtagcat tcaatgagac ccatatggaa attatgaaac cagaataatt
      121 tggtaattgt attagtttag aaatatttgt ttaagttatt gtgttcaatt tgttttgttt
      181 aggcagcaac tgatttggat gagaagagtt aaaaataggt tatttaatca ataaaagcaa
      241 tatgaaaagt attcctcagt agggtctaac aataaataca ctaatggtca aaataatata
      301 acttattcca gtctaaaaat attcacgaat tttcttctcc taaatactag aaattcttaa
      361 atttatcaaa aaccagaaaa aattgcaaca tctacagtca gtttaaattg tttttgaatt
      421 ttggtatttt ggtatttgag aaacgttcaa atcagatttt aaaaatattg gataggtatt
      481 ttcgcactca tacatctttg aaataaaaca ttaagttttt tctcaatacg tttgaaaaaa
      541 gtacagtact acatagtata tgcacacatt ttccaaatat atattatcaa cagaaaacta
      601 ttctcatcat tctattttcc caatattccc atccactttg aatcactaac aacaatcatg
      661 tgatttccta aatgttttcc ttttttcaaa ttgaagaaaa tcaacgctgc ggaacacatt
      721 ttcccgaaaa aagccgtccg agttgttccc gtccacacct tttttccctc tcaagagtta
      781 ttcttttgta gattctttgg atcattcact tgatagccta atagtgggcg tgtgtttgtc
      841 tgtgctccgt tacgtgaaaa acggaacaaa tattttttga aaaaaaaaca ttcgaatttt
      901 tccgtcgcgt ctcgaaatgc ccatttctcc tgctggtagg tttttataga cctctatttg
      961 aaaatttagt tatgaatgtt taaataagtg tctctaaaac tataaaaaac caatttttag
     1021 aggaatcttg gtagatgctc acttttcagc tcacattgat taaatcctca aaagttcaca
     1081 tataatcatt gaaattgttt tttgaaataa ttttttaaaa agttatgttt gactcattct
     1141 gttttggtta ctttggcaac tacctgaaca cagtctcctg tatcaattgc tcttaaactt
     1201 tttaatcgaa attttcagat tcaacaagca ttgctcgtca aaaacaacgt cgcatcgaac
     1261 cacttgtaat gcttgattct cgggattacg gtagttggag gaatggaaca actccatcgc
     1321 cgagcacaaa taacaaaagc cctccggtat ttcaaaatga gaagaagaga gttcattggc
     1381 ggaaataatt cttgtttcaa tttttttttg tattctctgt aaactgtgta catattttgt
     1441 aaattggatt actaatttaa aaataaatac ttgtaaaata aaatgaaata aataaataac
     1501 tgtaaaagcg tacttcaggt agatccaaaa tatttggagt ggatttaatt tttgggttca
     1561 ggtcaagtct gtgcggcaaa cggcacaagg ttcaagggac aagcaaccga aatcattgat
     1621 actaacgaaa agttgaaatg cagtaagaag atcaaggaat taccggacaa aattattgcc
     1681 aaggaacaaa ggcatacgtc tcagcttcat ttgcatgatc ctgttcaagt attccgtgac
     1741 atcatcaacc accatcacgt gtgtacctca taccagggct gtgcggcatc ctgaattttc
     1801 ggcgaacgtt cgccgacaca taaatttctc ataatttttc ggcgatcggc atttatcggc
     1861 acttttcggc atccttaaaa aaaaaatttt gtgttttttt ttcaattttc taaatctaaa
     1921 tagttgaatt ccaaagtttt ataaagagtg tctggtttga attttaaagc atagatcaat
     1981 ttcatattta aacgaccaga ctgttgggat agtcaaaaaa ggatcccaga ggcaagaatg
     2041 attcaaaata attgtgtcga ctaaaagaat atggctaata attcaaatat gttaattttt
     2101 gggaagtgtt agaaatacag gtgatttggg agtgttatga tacaagagac acagagatgt
     2161 ctttggttgt gttttatgac tataatgttt gtagatttat gtttcaacgg atgaatgtga
     2221 aaaacttgaa tgtatattcg attaaatttt taaaaaataa atttttcaca aatgccgaaa
     2281 aataccgaaa aaagtcgaac aaaagtcgac cgtttgccgc acatctctca tacatatgaa
     2341 cgtcatattt gaatattctc gtttaagctg atgtgcacgg catttggaat attacgggaa
     2401 ttgccaaaac catagaaaat attttcctaa cagcaatcga aactatttga ttccataact
     2461 ccaattccct tctttaaact agaaatttat aagcacatgc ctgttctaag tatccatcaa
     2521 tcaaatgcca agaaaccgca aaacaaggcg aaaattatca gaattctcaa caagtcatcc
     2581 gggcggctcc caagaggcag tgccgcggcg gcaacacgct catcttaaat gaaaacccaa
     2641 gaaagagacg ctatataatg ttttccgaaa tatcaatcat gcttctcctc acacatcagc
     2701 ttctcgtctt ctcgtcctac cgtagtcgct tatgaaccga gccgcctccc tagagactat
     2761 gggatcctca gcggtaagtg atgggctccg ccttgtaaaa tgcaacattt tccctcattc
     2821 aatatgagcc ggatgatttt tcgcatattt atcatttttg tttcaaagac tcctacagta
     2881 ctcttctaat cgtaaacctt ttcaattgac aataatttca gacCCGATCC ctATGGCTGA
     2941 GCTCATCTCC TCCGCTACCC GTTCCCTCTT CGCTGCTGGA GGAATCAACC CATGGCCGAA
     3001 CCCATACCAC CACGAGGACA TGGGATGCGG AGGAATGACC CCAACCGGAG AGTGCTTCTC
     3061 CACCGAGTGG TGGTGCGACC CATCCTACGG ACTCTCCGAC GCTGGATACG GATACTGCTT
     3121 CGTCGAGGCT ACCGGAGGTA AGTTTAAACT TGGACTTACT AACTAACCAT ACATATTTAA
     3181 ATTTTCAGGA TACCTCGTCG TCGGAGTCGA GAAGAAGCAG GCTTGGCTCC ACTCCCGTGG
     3241 AACCCCAGGA GAGAAGATCG GAGCTCAGGT CTGCCAGTGG ATCGCTTTCT CCATCGCTAT
     3301 CGCTCTCCTC ACCTTCTACG GATTCTCCGC TTGGAAGGCT ACCTGCGGAT GGGAGGAGGT
     3361 CTACGTCTGC TGCGTCGAGG TCCTCTTCGT CACCCTCGAG ATCTTCAAGG AGTTCTCCTC
     3421 CCCAGCTACC GTCTACCTCT CCACCGGAAA CCACGCTTAC TGCCTCCGTT ACTTCGAGTG
     3481 GCTCCTCTCC TGCCCAGTCA TCCTCATCCG TCTCTCCAAC CTCTCCGGAC TCAAGGTAAG
     3541 TTTAAACAGT TCGGTACTAA CTAACCCTGA TTATTTAAAT TTTCAGAACG ACTACTCCAA
     3601 GCGTACCATG GGACTCATCG TCTCCTGCGT CGGAATGATC GTCTTCGGAA TGGCTGCTGG
     3661 ACTCGCTACC GACTGGCTCA AGTGGCTCCT CTACATCGTC TCCTGCATCT ACGGAGGATA
     3721 CATGTACTTC CAGGCTGCTA AGTGCTACGT CGAGGCTAAC CACTCCGTCC CAAAGGGACA
     3781 CTGCCGTATG GTCGTCAAGC TCATGGCTTA CGCTTACTTC GCTTCCTGGG GTTCCTACCC
     3841 AATCCTCTGG GCTGTCGGAC CAGGTAAGTT TAAACATATA TATACTAACT AACGGATTAT
     3901 ATTTAAATTT TCAGAGGGAC TCCTCAAGCT CTCCCCATAC GCTAACTCCA TCGGACACTC
     3961 CATCTGCGAC ATCATCGCTA AGGAGTTCTG GACCTTCCTC GCTCACCACC TCCGTATCAA
     4021 GATCCACGAG CACATCCTCA TCCACGGAGA CATCCGTAAG ACCACCAAGA TGGAGATCGG
     4081 AGGAGAGGAG GTCGAGGTCG AGGAGTTCGT CGAGGAGGAG GACGAGGACA CCGTCGCGGT
     4141 ACCcgcggcc gccgtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     4201 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     4261 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     4321 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     4381 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     4441 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     4501 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     4561 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     4621 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     4681 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     4741 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     4801 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta
     4861 ataggaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata
     4921 ataggggccg ctgtcatcag agtaagttta aactgagttc tactaactaa cgagtaatat
     4981 ttaaattttc agcatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca
     5041 tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact
     5101 tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta
     5161 gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc
     5221 attaataata attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt
     5281 tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
     5341 tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat
     5401 gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa
     5461 gtgaaagttt atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg
     5521 tcaagatttc gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc
     5581 aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata
     5641 atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa
     5701 tataccaaac ataactgttt cctactagtc ggccgtacGG GCCCTTTCGT CTCGCGCGTT
     5761 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC
     5821 TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT
     5881 GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC
     5941 GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC
     6001 CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA
     6061 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT
     6121 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA
     6181 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT
     6241 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG
     6301 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT
     6361 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG
     6421 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG
     6481 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA
     6541 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG
     6601 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA
     6661 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC
     6721 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT
     6781 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA
     6841 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG
     6901 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA
     6961 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG
     7021 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT
     7081 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT
     7141 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA
     7201 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA
     7261 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT
     7321 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG
     7381 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA
     7441 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA
     7501 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG
     7561 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA
     7621 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA
     7681 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC
     7741 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC
     7801 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT
     7861 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT
     7921 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG
     7981 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA
     8041 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT
     8101 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG
     8161 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC
     8221 GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta aattttcagA
     8281 GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG GAAATGAAAT
//
