LOCUS       HULAa30F3           4784 bp ds-DNA     circular     09-NOV-2021
DEFINITION  .
KEYWORDS    "Gene" "Mutation" "Resistance" "Map" "Fluorophore" "name"          
            "preperation" "concentration (ng/µl)"
FEATURES             Location/Qualifiers
     misc_feature    329..333
                     /label="Shine Dalgarno"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             346..1131
                     /label="Translation 346-1131"
     misc_feature    352..369
                     /label="6xHis Tag"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     HeR             370..1131
                     /label="HULAa30F3"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             1869..2528
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      2626..3308
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3880..4758
                     /label="AraC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT
       61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA
      121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
      181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG
      241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG
      301 CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA TACCCATGGg tCATCATCAC
      361 CATCACCACG CTACTGATTC CGCGACGGAA AAGAAACTCA AGGGGCTCCG GACCTGGAAC
      421 ATTGTGGTGG GCCTGATACT GGCGGTGCAG GCGGTGCTGA TTGCCGTGCT GACCAACAGC
      481 TTCGCGCTGC CGGTGACCGC GACGTTTATG GAGGGGCCGC CGGGGACCGC GCCGGCGCTG
      541 CAGCATCTGT TTAATATCCA GACGGGGTGG GGCGTGTTCA TCTTCATGGC GATATCCGCC
      601 GGCGCGCTGC TGCTGATTGC CTCCCCGATG GTTTTCTCCT GGTACAAGCG CAATCTGCTG
      661 GAAAGCCGGA ACTACGGGCG CTGGATTGAG TATTTCTTCA GTTCCTCCGT CATGATCGTT
      721 CTGATATCCC AAATCTGCGG CATCAGCGAT ATTGCCGCGC TACTGGCGAT ATTCGGCATC
      781 AACGCGTCCA TGATACTCTT CGGCGCATTG CAGGAGAAGT ATGAGAAGCC GGGCAAGCCG
      841 AACCTGCTTT CGTTCTGGTT TGGCAGCTTC GCGGGGATAA TTCCCTGGAT TGCCGTTTTA
      901 ATCTACGTGG TATCCCCCGG CGTGGCGGCG GCGCCGCCCG CGTTTGTGTA CGGAATTGTA
      961 GTGGCGCTGT TCGTGTTCTT CAACTGCTTT GCGGTGAACA TGATTCTCCA GTACAAGCAG
     1021 ATAGGGCCGT GGAAAGACTA CCTGTTCGGC GAGAAGGTCT ATATAATACT GAGCCTGACC
     1081 GCGAAATCCC TGCTGGCATG GATGGTTTTC GCGAACGTAC TGGTCCTGTA AGTTTAAACG
     1141 GTCTCCAGCT TGGCTGTTTT GGCGGATGAG AGAAGATTTT CAGCCTGATA CAGATTAAAT
     1201 CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG CGGCAGTAGC GCGGTGGTCC
     1261 CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG CGCCGATGGT AGTGTGGGGT
     1321 CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA
     1381 GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT
     1441 CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC
     1501 CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA TGGCCTTTTT
     1561 GCGTTTCTAC AAACTCTTTT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
     1621 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC
     1681 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC
     1741 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT
     1801 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT
     1861 TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG
     1921 CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT
     1981 CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG
     2041 CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA
     2101 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG
     2161 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA
     2221 TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC
     2281 AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC
     2341 CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA
     2401 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA
     2461 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA
     2521 AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC
     2581 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC
     2641 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT
     2701 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC
     2761 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT
     2821 TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT
     2881 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG
     2941 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA
     3001 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA
     3061 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG
     3121 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG
     3181 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC
     3241 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA
     3301 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG
     3361 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC
     3421 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA
     3481 TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA
     3541 GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA
     3601 CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG
     3661 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA
     3721 GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCAG ATCAATTCGC GCGCGAAGGC
     3781 GAAGCGGCAT GCATAATGTG CCTGTCAAAT GGACGAAGCA GGGATTCTGC AAACCCTATG
     3841 CTACTCCGTC AAGCCGTCAA TTGTCTGATT CGTTACCAAT TATGACAACT TGACGGCTAC
     3901 ATCATTCACT TTTTCTTCAC AACCGGCACG GAACTCGCTC GGGCTGGCCC CGGTGCATTT
     3961 TTTAAATACC CGCGAGAAAT AGAGTTGATC GTCAAAACCA ACATTGCGAC CGACGGTGGC
     4021 GATAGGCATC CGGGTGGTGC TCAAAAGCAG CTTCGCCTGG CTGATACGTT GGTCCTCGCG
     4081 CCAGCTTAAG ACGCTAATCC CTAACTGCTG GCGGAAAAGA TGTGACAGAC GCGACGGCGA
     4141 CAAGCAAACA TGCTGTGCGA CGCTGGCGAT ATCAAAATTG CTGTCTGCCA GGTGATCGCT
     4201 GATGTACTGA CAAGCCTCGC GTACCCGATT ATCCATCGGT GGATGGAGCG ACTCGTTAAT
     4261 CGCTTCCATG CGCCGCAGTA ACAATTGCTC AAGCAGATTT ATCGCCAGCA GCTCCGAATA
     4321 GCGCCCTTCC CCTTGCCCGG CGTTAATGAT TTGCCCAAAC AGGTCGCTGA AATGCGGCTG
     4381 GTGCGCTTCA TCCGGGCGAA AGAACCCCGT ATTGGCAAAT ATTGACGGCC AGTTAAGCCA
     4441 TTCATGCCAG TAGGCGCGCG GACGAAAGTA AACCCACTGG TGATACCATT CGCGAGCCTC
     4501 CGGATGACGA CCGTAGTGAT GAATCTCTCC TGGCGGGAAC AGCAAAATAT CACCCGGTCG
     4561 GCAAACAAAT TCTCGTCCCT GATTTTTCAC CACCCCCTGA CCGCGAATGG TGAGATTGAG
     4621 AATATAACCT TTCATTCCCA GCGGTCGGTC GATAAAAAAA TCGAGATAAC CGTTGGCCTC
     4681 AATCGGCGTT AAACCCGCCA CCAGATGGGC ATTAAACGAG TATCCCGGCA GCAGGGGATC
     4741 ATTTTGCGCT TCAGCCATAC TTTTCATACT CCCGCCATTC AGAG
//