LOCUS       HULAb132A11         4781 bp ds-DNA     circular     09-NOV-2021
DEFINITION  .
KEYWORDS    "Gene" "Mutation" "Resistance" "Map" "Fluorophore" "name"          
            "preperation" "concentration (ng/µl)"
FEATURES             Location/Qualifiers
     misc_feature    329..333
                     /label="Shine Dalgarno"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    352..369
                     /label="6xHis Tag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    370..1128
                     /label="HULAb132A11"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             370..1128
                     /label="Translation 370-1128"
     CDS             1866..2525
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      2623..3305
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3877..4755
                     /label="AraC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT
       61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA
      121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
      181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG
      241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG
      301 CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA TACCCATGGg tCATCATCAC
      361 CATCACCACA CTAAAGAGAT CAATGATTCA AAGCTGCGCC GATACAACAT TATTGCCGGC
      421 GTATTCCATC TAGTTCAGAT GATTGCAGTC TTGGCTCTTG CCAATGACTT CACTCTTCCA
      481 ATTGTTGCTC GTTATATGTC CGGCCCTCCG GGAAGCACCT TCGCAGAGCC AATCACCTTG
      541 CTAAACACTC CAGTCGGAAT CGGCGTCGCT CTATTCCTTG GTCTTTCAGC TTTCTTCCAC
      601 TTCTTAGTGG CTTCACCACA GTTCTTCTCA CGCTACAGCG CAGGCCTTGC ACAAAACCGC
      661 AATTACTTCC GCTGGGTTGA GTACTCAATC AGCTCATCTG TAATGATCGT GTTGATCGCT
      721 CAGATCTGCG GAGTCTCAGA TGTCGTCGCG ATTATTTCAA TCTTTGGCGT GAACGCTTCA
      781 ATGATCTTGT TTGGTTGGCT ACAAGAGAAG TACGAGACAC CAGGAAATGG CGGATGGATT
      841 CCTTTCATCT TTGGTTGCAT CACAGGAATC ATCCCTTGGC TAGCTTTGGT TTTCTATGTT
      901 TTCTCAATCG GCGGTCCATC AGAGGCATCA GCTCCGGCAT TTGTTTACGG AATCGTTTTC
      961 TCACTCTTCG TACTTTTCAA TACCTTTGCA GTCGTTCAAT ATCTGCAGTA CAAGAAGGTT
     1021 GGAAAGTGGT CTGACTACCT AAGTGGAGAA AAGACATACA TAACTCTCTC TTTGATCGCT
     1081 AAATCAGCAC TTGCTTGGCA GGTTTTTGTC GGCACTTTGA TTGAGTAAGT TTAAACGGTC
     1141 TCCAGCTTGG CTGTTTTGGC GGATGAGAGA AGATTTTCAG CCTGATACAG ATTAAATCAG
     1201 AACGCAGAAG CGGTCTGATA AAACAGAATT TGCCTGGCGG CAGTAGCGCG GTGGTCCCAC
     1261 CTGACCCCAT GCCGAACTCA GAAGTGAAAC GCCGTAGCGC CGATGGTAGT GTGGGGTCTC
     1321 CCCATGCGAG AGTAGGGAAC TGCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC
     1381 TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG
     1441 CCGGGAGCGG ATTTGAACGT TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC AGGACGCCCG
     1501 CCATAAACTG CCAGGCATCA AATTAAGCAG AAGGCCATCC TGACGGATGG CCTTTTTGCG
     1561 TTTCTACAAA CTCTTTTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA
     1621 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC
     1681 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC
     1741 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA
     1801 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC
     1861 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG
     1921 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC
     1981 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA
     2041 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG
     2101 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC
     2161 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG
     2221 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT
     2281 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG
     2341 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG
     2401 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC
     2461 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC
     2521 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT
     2581 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT
     2641 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT
     2701 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG
     2761 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA
     2821 GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA
     2881 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG
     2941 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
     3001 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT
     3061 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA
     3121 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC
     3181 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG
     3241 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG
     3301 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT
     3361 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC
     3421 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC
     3481 GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA
     3541 CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG
     3601 GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT
     3661 GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG
     3721 GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCAGATC AATTCGCGCG CGAAGGCGAA
     3781 GCGGCATGCA TAATGTGCCT GTCAAATGGA CGAAGCAGGG ATTCTGCAAA CCCTATGCTA
     3841 CTCCGTCAAG CCGTCAATTG TCTGATTCGT TACCAATTAT GACAACTTGA CGGCTACATC
     3901 ATTCACTTTT TCTTCACAAC CGGCACGGAA CTCGCTCGGG CTGGCCCCGG TGCATTTTTT
     3961 AAATACCCGC GAGAAATAGA GTTGATCGTC AAAACCAACA TTGCGACCGA CGGTGGCGAT
     4021 AGGCATCCGG GTGGTGCTCA AAAGCAGCTT CGCCTGGCTG ATACGTTGGT CCTCGCGCCA
     4081 GCTTAAGACG CTAATCCCTA ACTGCTGGCG GAAAAGATGT GACAGACGCG ACGGCGACAA
     4141 GCAAACATGC TGTGCGACGC TGGCGATATC AAAATTGCTG TCTGCCAGGT GATCGCTGAT
     4201 GTACTGACAA GCCTCGCGTA CCCGATTATC CATCGGTGGA TGGAGCGACT CGTTAATCGC
     4261 TTCCATGCGC CGCAGTAACA ATTGCTCAAG CAGATTTATC GCCAGCAGCT CCGAATAGCG
     4321 CCCTTCCCCT TGCCCGGCGT TAATGATTTG CCCAAACAGG TCGCTGAAAT GCGGCTGGTG
     4381 CGCTTCATCC GGGCGAAAGA ACCCCGTATT GGCAAATATT GACGGCCAGT TAAGCCATTC
     4441 ATGCCAGTAG GCGCGCGGAC GAAAGTAAAC CCACTGGTGA TACCATTCGC GAGCCTCCGG
     4501 ATGACGACCG TAGTGATGAA TCTCTCCTGG CGGGAACAGC AAAATATCAC CCGGTCGGCA
     4561 AACAAATTCT CGTCCCTGAT TTTTCACCAC CCCCTGACCG CGAATGGTGA GATTGAGAAT
     4621 ATAACCTTTC ATTCCCAGCG GTCGGTCGAT AAAAAAATCG AGATAACCGT TGGCCTCAAT
     4681 CGGCGTTAAA CCCGCCACCA GATGGGCATT AAACGAGTAT CCCGGCAGCA GGGGATCATT
     4741 TTGCGCTTCA GCCATACTTT TCATACTCCC GCCATTCAGA G
//