LOCUS       HULAa3G5            4826 bp ds-DNA     circular     09-NOV-2021
DEFINITION  .
KEYWORDS    "Gene" "Mutation" "Resistance" "Map" "Fluorophore" "name"          
            "preperation" "concentration (ng/µl)"
FEATURES             Location/Qualifiers
     misc_feature    329..333
                     /label="Shine Dalgarno"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    352..369
                     /label="6xHis Tag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    370..1173
                     /label="HULAa3G5"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             370..1173
                     /label="Translation 370-1173"
     CDS             1911..2570
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      2668..3350
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(3922..3924)
                     /label="Translation 3924-3922"
     misc_feature    3922..4800
                     /label="AraC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(3925..4800)
                     /label="Translation 4800-3925"
ORIGIN
        1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT
       61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA
      121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
      181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG
      241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG
      301 CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA TACCCATGGg tCATCATCAC
      361 CATCACCACG CTAAAGTTGA AAGAACTCCA GATCAATACA TTTCAAGATT GCGCTCCTAC
      421 AACGTCGTAG CTGGGTTTCT ACACCTAGCG CAGGCTGCGG GACTTACCTA CGTCTTGACG
      481 TTACTTGAAA CACAAATTTT GTTTCCGGTC ACAATTGACT ATCCGACAGG CCCTCCGGGA
      541 GTGCCGGTTC CGCCAGAGCG AGTCGAACTT TTTGACATCA ACATCGGAGC GGGAGTCATC
      601 GGCTTCTTAC TACTTTCCGC TTTCTTTCAT TTTCTGATTG CATCGCCGCT GTTTTTCCAG
      661 CGCTATAAAA ATGGCCTAAA ACAAAATCAC AATTACTTCC GCTGGGTTGA GTATTCGCTC
      721 TCATCCTCAG TAATGATCTG GCTGATTGCG CAGCTGACAG GCGTTTCAGA CTTTGCAGCG
      781 CTGGCCGGAA TCTTTGCCGT CAACGCTTCA ATGATTTTCT TTGGTGCTTT GCAGGAGAAG
      841 TATGAGACTC CAGGTAACGG CAAGGGACTT CCATTCATCA TGGGAAGCAT GACCGGTATC
      901 GTTCCCTGGC TGATCATCGT TGTCTACACA ATTCAGCCTG GTAGCGAATC AGCTGCCGAG
      961 ATTCCTGGCT TCGTTTATGG AATTATCGTT GCGCTATTCA TCTTCTTCAA CACCTTTGCC
     1021 TTCAATCAGG CATTCCAATA CAAGAAGATT GGACCTTGGA GAAACTATCT ATATGGCGAA
     1081 GCAACCTACA TAACGCTAAG CCTGGTAGCA AAGTCGATAT TGGCTTGGCA GGTATTCTCG
     1141 GGAGCAATCA TCCCGGCGAT TGCCAGCACA TAAGTTTAAA CGGTCTCCAG CTTGGCTGTT
     1201 TTGGCGGATG AGAGAAGATT TTCAGCCTGA TACAGATTAA ATCAGAACGC AGAAGCGGTC
     1261 TGATAAAACA GAATTTGCCT GGCGGCAGTA GCGCGGTGGT CCCACCTGAC CCCATGCCGA
     1321 ACTCAGAAGT GAAACGCCGT AGCGCCGATG GTAGTGTGGG GTCTCCCCAT GCGAGAGTAG
     1381 GGAACTGCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT
     1441 ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGGG AGCGGATTTG
     1501 AACGTTGCGA AGCAACGGCC CGGAGGGTGG CGGGCAGGAC GCCCGCCATA AACTGCCAGG
     1561 CATCAAATTA AGCAGAAGGC CATCCTGACG GATGGCCTTT TTGCGTTTCT ACAAACTCTT
     1621 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT
     1681 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC
     1741 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA
     1801 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA
     1861 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT
     1921 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG
     1981 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC
     2041 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA
     2101 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT
     2161 TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG
     2221 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA
     2281 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG
     2341 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG
     2401 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG
     2461 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG
     2521 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG
     2581 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA
     2641 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT
     2701 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC
     2761 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
     2821 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
     2881 CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
     2941 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
     3001 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
     3061 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
     3121 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
     3181 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
     3241 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
     3301 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
     3361 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG
     3421 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG
     3481 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA
     3541 CGCATCTGTG CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG
     3601 CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC
     3661 CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC
     3721 TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC
     3781 ACCGAAACGC GCGAGGCAGC AGATCAATTC GCGCGCGAAG GCGAAGCGGC ATGCATAATG
     3841 TGCCTGTCAA ATGGACGAAG CAGGGATTCT GCAAACCCTA TGCTACTCCG TCAAGCCGTC
     3901 AATTGTCTGA TTCGTTACCA ATTATGACAA CTTGACGGCT ACATCATTCA CTTTTTCTTC
     3961 ACAACCGGCA CGGAACTCGC TCGGGCTGGC CCCGGTGCAT TTTTTAAATA CCCGCGAGAA
     4021 ATAGAGTTGA TCGTCAAAAC CAACATTGCG ACCGACGGTG GCGATAGGCA TCCGGGTGGT
     4081 GCTCAAAAGC AGCTTCGCCT GGCTGATACG TTGGTCCTCG CGCCAGCTTA AGACGCTAAT
     4141 CCCTAACTGC TGGCGGAAAA GATGTGACAG ACGCGACGGC GACAAGCAAA CATGCTGTGC
     4201 GACGCTGGCG ATATCAAAAT TGCTGTCTGC CAGGTGATCG CTGATGTACT GACAAGCCTC
     4261 GCGTACCCGA TTATCCATCG GTGGATGGAG CGACTCGTTA ATCGCTTCCA TGCGCCGCAG
     4321 TAACAATTGC TCAAGCAGAT TTATCGCCAG CAGCTCCGAA TAGCGCCCTT CCCCTTGCCC
     4381 GGCGTTAATG ATTTGCCCAA ACAGGTCGCT GAAATGCGGC TGGTGCGCTT CATCCGGGCG
     4441 AAAGAACCCC GTATTGGCAA ATATTGACGG CCAGTTAAGC CATTCATGCC AGTAGGCGCG
     4501 CGGACGAAAG TAAACCCACT GGTGATACCA TTCGCGAGCC TCCGGATGAC GACCGTAGTG
     4561 ATGAATCTCT CCTGGCGGGA ACAGCAAAAT ATCACCCGGT CGGCAAACAA ATTCTCGTCC
     4621 CTGATTTTTC ACCACCCCCT GACCGCGAAT GGTGAGATTG AGAATATAAC CTTTCATTCC
     4681 CAGCGGTCGG TCGATAAAAA AATCGAGATA ACCGTTGGCC TCAATCGGCG TTAAACCCGC
     4741 CACCAGATGG GCATTAAACG AGTATCCCGG CAGCAGGGGA TCATTTTGCG CTTCAGCCAT
     4801 ACTTTTCATA CTCCCGCCAT TCAGAG
//