LOCUS       Exported                7239 bp DNA     circular SYN 30-NOV-2021
DEFINITION  mito 144c, A1341T, N1342S, E1370K, T1380I COX8a MTS_3xFLAG_11696  
            Left-TALE_2aa_DddE(41-148 )_1xUGI_ATP5B 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Guilinger JP Pattanayak V Reyon D Tsai SQ Sander JD Joung JK Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58 
            http://www.snapgene.com
REFERENCE   3  (bases 1 to 7239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Exported 
            Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com"
COMMENT     Imported From
            Path: /Users/bmok/Downloads
            Filename: addgene-plasmid-51444-sequence-78455.gbk
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="synthetic DNA construct"
                     /mol_type="genomic DNA"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="color: black; sequence: GCGGCATCAGAGCAGATTGT"
     primer_bind     198..213
                     /label=BM719
                     /note="color: black; sequence: GCGCTGCTTCGCGATG"
     primer_bind     209..229
                     /label=BM525
                     /note="color: black; sequence: CGATGTACGGGCCAGATATAC"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     365..379
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     complement(419..435)
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     687..706
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     complement(687..704)
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     770..790
                     /label=CMV-F
                     /note="color: black; sequence: GCAAATGGGCGGTAGGCGTGT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     864..883
                     /label=T7
                     /note="color: black; sequence: AATACGACTCACTATAGGGA"
     misc_feature    898..903
                     /label=Kozak sequence
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    904..906
                     /label=START
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             910..993
                     
                     /note="/created by=bmok"
                     /note="color: #993366; direction: RIGHT"
     CDS             994..1059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #993366"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(1036..1067)
                     /label=BM850
                     /note="color: black; sequence: 
                     ATGTCCATCTTGTCATCGTCATCCTTGTAATC"
     primer_bind     complement(1045..1067)
                     
                     /note="color: black; sequence: ATGTCCATCTTGTCATCGTCATC"
     primer_bind     1055..1079
                     /label=BM851
                     /note="color: black; sequence: ACAAGATGGACATCGCGGATCTGCG"
     CDS             1063..1476
                     
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(1446..1464)
                     /label=BM1529
                     /note="color: black; sequence: ACCGGTCAGAGCATTACGC"
     primer_bind     1451..1476
                     /label=BM1892
                     /note="color: black; sequence: 
                     ATGCTCTGACCGGTGCGCCGCTGAACCTGACACCCGAGCAGG"
     CDS             1477..2556
                     /label=m.11696-Left TALE
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1477
                     /label=C>T
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1479
                     /label=G>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1482
                     /label=C>G
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1485
                     /label=T>G
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1488
                     /label=G>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1491
                     /label=G>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1494
                     /label=G>T
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1497
                     /label=G>T
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    complement(1500..1517)
                     /label=motif
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    2556
                     /label=G>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2557..2679
                     
                     /note="/created by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    2568
                     /label=G>T
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     primer_bind     complement(2662..2683)
                     /label=BM905
                     /note="color: black; sequence: ATCCACCCAGGCCTTTCTTCAC"
     CDS             2686..3009
                     /label= DddE(41-148) DddA6
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    2713..2754
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2725..2744)
                     /label=BM476
                     /note="color: black; sequence: CCATTGTAAGCTGGCAGCTG"
     primer_bind     complement(2739..2757)
                     /label=BM1852
                     /note="color: black; sequence: ACCCACGGTCTGACCATTG"
     misc_feature    2746..2748
                     /label=Q1310R
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     2749..2779
                     /label=BM1850
                     /note="color: black; sequence: 
                     ACCGTGGGTGCCTTCTACTATGTGAACGACG"
     primer_bind     2759..2844
                     /label=BM1849
                     /note="color: black; sequence: 
                     GCCTTCTACTATGTGAACGACGCGGGCGGTCTGGAGAGCAAGGTGTTTAGCAGCGGCGG
                     TCCAACCCCGTACCCAAACTATGCCAAT"
     misc_feature    2773..2784
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2806..2817
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2806..2808
                     /label=S1330I
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2824..2847)
                     /label=BM1851
                     /note="color: black; sequence: ACGGGCATTGGCATAGTTTGGGTACGG"
     primer_bind     2842..2859
                     /label=BM1853
                     /note="color: black; sequence: AATGCCCGTCATGTGGAG"
     regulatory      2851..2853
                     /label=His96-chelate to zinc
                     /note="/created by=bmok"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2857..2859
                     
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2890..2898
                     /label=loop (SPLIT)
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     regulatory      2935..2937
                     
                     /note="/created by=bmok"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2956..2958
                     /label=T1380I
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2965..2976
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2992..3009
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2997..3038)
                     /label=BM1095
                     /note="color: black; sequence: 
                     ATGTCGCTCAGATTAGTTGAGCCGCCAGAACCTTCCGGCGGC"
     misc_feature    3007..3009
                     
                     /note="Geneious type: Sequence difference"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     3021..3043
                     /label=BM941
                     /note="color: black; sequence: AACTAATCTGAGCGACATCATTG"
     CDS             3022..3270
                     /label=UGI
                     /note="/created by=kzhao"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(3248..3274)
                     /label=BM1032
                     /note="color: black; sequence: ATCACAGCATCTTGATCTTATTCTCGC"
     primer_bind     3262..3291
                     
                     /note="color: black; sequence: 
                     AAGATGCTGTGATAAGGGGTCTTTGTCCTC"
     primer_bind     3273..3296
                     /label=BM855
                     /note="color: black; sequence: ATAAGGGGTCTTTGTCCTCTGTAC"
     3'UTR           3277..3424
                     /label=ATP5B 3'UTR
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(3401..3427)
                     /label=BM870
                     /note="color: black; sequence: AAGTTCTGAGGGGTGTACATTTTATTG"
     terminator      3402..3406
                     
                     /note="/created by=bmok"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3414..3444
                     /label=BM871
                     /note="color: black; sequence: 
                     ACCCCTCAGAACTTCTGAGGCGGAAAGAACC"
     rep_origin      3470..3898
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow  
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(3479..3495)
                     /label=BM889
                     /note="color: black; sequence: GCTTAATGCGCCGCTAC"
     primer_bind     complement(3558..3577)
                     /label=F1ori-R
                     /note="color: black; sequence: AAGGGAAGAAAGCGAAAGGA"
     primer_bind     3768..3789
                     /label=F1ori-F
                     /note="color: black; sequence: TGGACTCTTGTTCCAAACTGGA"
     primer_bind     complement(3908..3928)
                     /label=pBABE 3'
                     /note="color: black; sequence: CACCCTAACTGACACACATTC"
     promoter        3912..4242
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4093..4228
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     4156..4175
                     /label=SV40pro-F
                     /note="color: black; sequence: ATTTATGCAGAGGCCGAGGC"
     primer_bind     complement(4177..4189)
                     /label=SV40pro-F
                     /note="color: black; sequence: ATTTATGCAGAGGCCGAGGC"
     promoter        4290..4337
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4356..4754
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #993366"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4912..5033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(4950..4969)
                     /label=SV40pA-R
                     /note="color: black; sequence: TGAAATTTGTGATGCTATTG"
     primer_bind     5004..5023
                     /label=EBV-rev
                     /note="color: black; sequence: TGGTTTGTCCAAACTCATCA"
     primer_bind     complement(5083..5099)
                     /label=M13 rev
                     /note="color: black; sequence: ACAGGAAACAGCTATGA"
     primer_bind     complement(5083..5099)
                     /label=M13 Reverse
                     /note="color: black; sequence: ACAGGAAACAGCTATGA"
     primer_bind     complement(5096..5118)
                     /label=M13/pUC Reverse
                     /note="color: black; sequence: GAGCGGATAACAATTTCACACAG"
     protein_bind    5106..5122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to  
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b; direction: RIGHT"
     promoter        complement(5130..5160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    5175..5196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     complement(5207..5223)
                     
                     /note="color: black; sequence: GAAAGCGGGCAGTGAGC"
     primer_bind     complement(5314..5331)
                     /label=L4440
                     /note="color: black; sequence: CAGCGAGTCAGTGAGCGA"
     rep_origin      complement(5484..6072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(5565..5584)
                     /label=pBR322ori-F
                     /note="color: black; sequence: GGGGAAACGCCTGGTATCTT"
     CDS             complement(6243..7103)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6867..6886
                     /label=Amp-R
                     /note="color: black; sequence: TAATACCGCGCCACATAGCA"
     promoter        complement(7104..7208)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 accatggcct ccgttctgac cccgctgctg ctgcgtggcc tgaccggctc cgctcgtcgt
      961 ctgccagttc cgcgtgcgaa aatccattcc ctggactaca aagaccatga cggtgattat
     1021 aaagatcatg acatcgatta caaggatgac gatgacaaga tggacatcgc ggatctgcgt
     1081 accctgggtt acagccagca gcagcaggag aagatcaagc cgaaggtgcg cagcaccgtg
     1141 gctcagcacc acgaagccct ggtgggccac ggtttcaccc acgctcacat cgtggccctg
     1201 agccagcacc cagccgcgct gggcaccgtg gccgtgaaat atcaggacat gattgctgcc
     1261 ctgccagagg ccacccatga agctattgtg ggcgtgggca agcagtggag cggtgctcgt
     1321 gcgctggagg cgctgctgac cgtggctggt gaactgcgtg gtccgccgct gcagctggat
     1381 accggtcagc tgctgaaaat tgccaaacgt ggcggtgtga ccgcggtgga agccgtgcat
     1441 gcttggcgta atgctctgac cggtgcgccg ctgaacttaa cgccggaaca agttgttgcc
     1501 atcgccagca acaacggcgg caagcaagct ctggaaaccg tgcaggccct gcttccagtc
     1561 ctgtgtcagg cccacggcct gacccctcag caggtggtcg ccatcgcctc taacatcggc
     1621 ggaaagcagg ctctggagac cgtgcagcgg ctgctgcccg tgctgtgcca ggcccacggc
     1681 ctgacacctc agcaggtggt ggccattgct agcaacatcg gaggcaagca ggccctggaa
     1741 accgtgcaaa gactgctgcc cgtgctgtgc caggcccacg gcctcacacc cgagcaagtg
     1801 gtggccatcg ctagcaacaa cggcggcaag caggctctcg agacagtcca agctctgctg
     1861 ccagtgctgt gccaagctca cggcctgaca cccgagcagg tggtcgccat cgcctcccac
     1921 gatggcggca aacaggctct ggaaacagtg caggccctgc tgcctgtgct gtgccaggcc
     1981 cacggcctga cccctgagca ggtggtggcc atcgccagca atggcggagg caagcaggcc
     2041 ctggaaaccg tgcaggctct gctgcctgtg ctgtgtcaag cccatggcct gacccctgag
     2101 caggtggtgg ctatcgcctc taatggcgga ggcaagcagg ccctggaaac cgtgcagaga
     2161 ctgctgcctg tgctgtgcca ggcccatgga ctgacccctg agcaggttgt tgcaatcgcc
     2221 agccacgacg gcggcaaaca agccctggaa accgtgcagg ccctgctgcc cgtgctgtgc
     2281 caggcccacg gactgacccc acagcaggtt gtggccatcg ccagcaacat cggcggcaag
     2341 caggccctgg aaacagtgca gcggctgctg cctgtgctgt gtcaggccca cggcctgacc
     2401 cctcaacagg tggtggccat cgccagccac gacggcggca agcaggctct tgagaccgtg
     2461 cagagactgc tgcccgtgct gtgccaggcc cacggactga cacctcagca ggtggtggcc
     2521 atcgcctccc acgacggcgg gagacctgcc ctcgaaagca ttgtggctca gctgagccgt
     2581 ccagacccgg cgctggcggc tctgaccaac gatcacctgg tggcgctggc ttgcctgggc
     2641 ggtcgtccgg ccctggatgc cgtgaagaaa ggcctgggtg gatccggcag ctacgccctg
     2701 ggtccgtatc agattagcgc cccgcagctg ccagcttaca atggtcggac cgtgggtacc
     2761 ttctactatg tgaacgacgc gggcggtctg gagagcaagg tgtttatcag cggcggtcca
     2821 accccgtacc caaactatgc caatgccggt catgtggagg gtcagagcgc cctgttcatg
     2881 cgtgataacg gcatcagcga gggtctggtg ttccacaaca acccggaagg cacctgcggt
     2941 ttttgcgtga acatgatcga gaccctgctg ccggaaaacg cgaaaatgac cgtggtgccg
     3001 ccggaaggtt ctggcggctc aactaatctg agcgacatca ttgagaagga gactgggaaa
     3061 cagctggtca ttcaggagtc catcctgatg ctgcctgagg aggtggagga agtgatcggc
     3121 aacaagccag agtctgacat cctggtgcac accgcctacg acgagtccac agatgagaat
     3181 gtgatgctgc tgacctctga cgcccccgag tataagcctt gggccctggt catccaggat
     3241 tctaacggcg agaataagat caagatgctg tgataagggg tctttgtcct ctgtactgtc
     3301 tctctccttg cccctaaccc aaaaagcttc atttttctgt gtaggctgca caagagcctt
     3361 gattgaagat atattctttc tgaacagtat ttaaggtttc caataaaatg tacacccctc
     3421 agaacttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     3481 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3541 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3601 tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg
     3661 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     3721 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     3781 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     3841 gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     3901 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga
     3961 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     4021 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     4081 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4141 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     4201 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     4261 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     4321 gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag
     4381 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     4441 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     4501 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     4561 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     4621 cctgcggacg gtgtcgacag gtgcttctcg atctgcatcc tgggatcaaa gcgatagtga
     4681 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     4741 tgtgggaggg ctaagcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     4801 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4861 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     4921 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4981 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5041 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5101 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5161 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5221 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5281 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5341 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5401 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5461 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5521 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5581 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5641 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     5701 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5761 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5821 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5881 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     5941 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6001 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6061 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6121 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6181 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6241 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6301 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6361 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6421 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6481 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6541 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6601 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6661 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6721 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6781 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6841 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6901 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6961 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7021 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7081 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7141 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7201 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
