LOCUS       Exported                8013 bp DNA     circular SYN 30-NOV-2021
DEFINITION  mitoTALE 144a-dead-mcherry , S1330I, A1341V, N1342S, E1370K, T1380I 
            COX8a MTS_3xFLAG_11696  Left-TALE_2aa_DddE(41-148 )_1xUGI_ATP5B 
            3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Guilinger JP Pattanayak V Reyon D Tsai SQ Sander JD Joung JK Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58 
            http://www.snapgene.com
REFERENCE   3  (bases 1 to 8013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Exported 
            Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com"
COMMENT     Imported From
            Path: /Users/bmok/Downloads
            Filename: addgene-plasmid-51444-sequence-78455.gbk
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="synthetic DNA construct"
                     /mol_type="genomic DNA"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="color: black; sequence: GCGGCATCAGAGCAGATTGT"
     primer_bind     198..213
                     /label=BM719
                     /note="color: black; sequence: GCGCTGCTTCGCGATG"
     primer_bind     209..229
                     /label=BM525
                     /note="color: black; sequence: CGATGTACGGGCCAGATATAC"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     365..379
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     complement(419..435)
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     687..706
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     complement(687..704)
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     770..790
                     /label=CMV-F
                     /note="color: black; sequence: GCAAATGGGCGGTAGGCGTGT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     864..883
                     /label=T7
                     /note="color: black; sequence: AATACGACTCACTATAGGGA"
     misc_feature    898..903
                     /label=Kozak sequence
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    904..906
                     /label=START
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             910..993
                     
                     /note="/created by=bmok"
                     /note="color: #993366; direction: RIGHT"
     CDS             994..1059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #993366"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(1036..1067)
                     /label=BM850
                     /note="color: black; sequence: 
                     ATGTCCATCTTGTCATCGTCATCCTTGTAATC"
     primer_bind     complement(1045..1067)
                     
                     /note="color: black; sequence: ATGTCCATCTTGTCATCGTCATC"
     primer_bind     1055..1079
                     /label=BM851
                     /note="color: black; sequence: ACAAGATGGACATCGCGGATCTGCG"
     CDS             1063..1476
                     
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(1446..1464)
                     /label=BM1529
                     /note="color: black; sequence: ACCGGTCAGAGCATTACGC"
     primer_bind     1451..1476
                     /label=BM1892
                     /note="color: black; sequence: 
                     ATGCTCTGACCGGTGCGCCGCTGAACCTGACACCCGAGCAGG"
     CDS             1477..2556
                     /label=m.11696-Left TALE
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1477
                     /label=C>T
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1479
                     /label=G>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1482
                     /label=C>G
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1485
                     /label=T>G
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1488
                     /label=G>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1491
                     /label=G>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1494
                     /label=G>T
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1497
                     /label=G>T
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    complement(1500..1517)
                     /label=motif
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    2556
                     /label=G>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2557..2679
                     
                     /note="/created by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    2568
                     /label=G>T
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     primer_bind     complement(2662..2683)
                     /label=BM905
                     /note="color: black; sequence: ATCCACCCAGGCCTTTCTTCAC"
     CDS             2686..3009
                     /label= DddE(41-148) dead
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    2713..2754
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2725..2744)
                     /label=BM476
                     /note="color: black; sequence: CCATTGTAAGCTGGCAGCTG"
     primer_bind     complement(2739..2757)
                     /label=BM1852
                     /note="color: black; sequence: ACCCACGGTCTGACCATTG"
     primer_bind     2749..2779
                     /label=BM1850
                     /note="color: black; sequence: 
                     ACCGTGGGTGCCTTCTACTATGTGAACGACG"
     primer_bind     2759..2844
                     /label=BM1849
                     /note="color: black; sequence: 
                     GCCTTCTACTATGTGAACGACGCGGGCGGTCTGGAGAGCAAGGTGTTTAGCAGCGGCGG
                     TCCAACCCCGTACCCAAACTATGCCAAT"
     misc_feature    2773..2784
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2806..2817
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2824..2847)
                     /label=BM1851
                     /note="color: black; sequence: ACGGGCATTGGCATAGTTTGGGTACGG"
     regulatory      2851..2853
                     /label=His96-chelate to zinc
                     /note="/created by=bmok"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2857..2859
                     
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2890..2898
                     /label=loop (SPLIT)
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     regulatory      2935..2937
                     
                     /note="/created by=bmok"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2965..2976
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2992..3009
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2997..3038)
                     /label=BM1095
                     /note="color: black; sequence: 
                     ATGTCGCTCAGATTAGTTGAGCCGCCAGAACCTTCCGGCGGC"
     misc_feature    3007..3009
                     
                     /note="Geneious type: Sequence difference"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(3017..3038)
                     /label=BM1914
                     /note="color: black; sequence: ATGTCGCTCAGATTAGTTGAGC"
     primer_bind     3021..3043
                     /label=BM941
                     /note="color: black; sequence: AACTAATCTGAGCGACATCATTG"
     CDS             3022..3270
                     /label=UGI
                     /note="/created by=kzhao"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(3248..3309)
                     /label=BM2097
                     /note="color: black; sequence: 
                     AGCCTGCTTCAGCAGGCTGAAGTTAGTAGCTCCGCTTCCCAGCATCTTGATCTTATTCT
                     CGC"
     misc_feature    3271..3336
                     /label=from pPC1349
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             3280..3336
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1  
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding 
                     separatepolypeptides."
                     /note="color: #993366"
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     3292..3352
                     /label=BM2099
                     /note="color: black; sequence: 
                     AGCCTGCTGAAGCAGGCTGGAGACGTGGAGGAGAACCCTGGACCTATGGTGTCCAAGGG
                     CG"
     misc_feature    3337..4044
                     /label=mCherry from pPC1101
                     /note="Geneious type: CDS"
                     /note="/ApEinfo_fwdcolor=#d59687"
                     /note="/ApEinfo_revcolor=#d59687"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(3687..3705)
                     /label=BM2101
                     /note="color: black; sequence: AAATTCTCCGTCCTGCAGG"
     primer_bind     3714..3735
                     /label=PC1092
                     /note="color: black; sequence: GGTTAAGTTGAGAGGCACAAAC"
     primer_bind     complement(4024..4059)
                     /label=BM2164
                     /note="color: black; sequence: 
                     AAAGACCCCTTATCACTTGTAAAGCTCGTCCATCCC"
     primer_bind     4047..4070
                     /label=BM855
                     /note="color: black; sequence: ATAAGGGGTCTTTGTCCTCTGTAC"
     3'UTR           4051..4198
                     /label=ATP5B 3'UTR
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(4175..4201)
                     /label=BM870
                     /note="color: black; sequence: AAGTTCTGAGGGGTGTACATTTTATTG"
     terminator      4176..4180
                     
                     /note="/created by=bmok"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     4188..4218
                     /label=BM871
                     /note="color: black; sequence: 
                     ACCCCTCAGAACTTCTGAGGCGGAAAGAACC"
     rep_origin      4244..4672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow  
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(4253..4269)
                     /label=BM889
                     /note="color: black; sequence: GCTTAATGCGCCGCTAC"
     primer_bind     complement(4332..4351)
                     /label=F1ori-R
                     /note="color: black; sequence: AAGGGAAGAAAGCGAAAGGA"
     primer_bind     4542..4563
                     /label=F1ori-F
                     /note="color: black; sequence: TGGACTCTTGTTCCAAACTGGA"
     primer_bind     complement(4682..4702)
                     /label=pBABE 3'
                     /note="color: black; sequence: CACCCTAACTGACACACATTC"
     promoter        4686..5016
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4867..5002
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     4930..4949
                     /label=SV40pro-F
                     /note="color: black; sequence: ATTTATGCAGAGGCCGAGGC"
     primer_bind     complement(4951..4963)
                     /label=SV40pro-F
                     /note="color: black; sequence: ATTTATGCAGAGGCCGAGGC"
     promoter        5064..5111
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5130..5528
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #993366"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5686..5807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(5724..5743)
                     /label=SV40pA-R
                     /note="color: black; sequence: TGAAATTTGTGATGCTATTG"
     primer_bind     5778..5797
                     /label=EBV-rev
                     /note="color: black; sequence: TGGTTTGTCCAAACTCATCA"
     primer_bind     complement(5857..5873)
                     /label=M13 rev
                     /note="color: black; sequence: ACAGGAAACAGCTATGA"
     primer_bind     complement(5857..5873)
                     /label=M13 Reverse
                     /note="color: black; sequence: ACAGGAAACAGCTATGA"
     primer_bind     complement(5870..5892)
                     /label=M13/pUC Reverse
                     /note="color: black; sequence: GAGCGGATAACAATTTCACACAG"
     protein_bind    5880..5896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to  
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b; direction: RIGHT"
     promoter        complement(5904..5934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    5949..5970
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     complement(5981..5997)
                     
                     /note="color: black; sequence: GAAAGCGGGCAGTGAGC"
     primer_bind     complement(6088..6105)
                     /label=L4440
                     /note="color: black; sequence: CAGCGAGTCAGTGAGCGA"
     rep_origin      complement(6258..6846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(6339..6358)
                     /label=pBR322ori-F
                     /note="color: black; sequence: GGGGAAACGCCTGGTATCTT"
     CDS             complement(7017..7877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7641..7660
                     /label=Amp-R
                     /note="color: black; sequence: TAATACCGCGCCACATAGCA"
     promoter        complement(7878..7982)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 accatggcct ccgttctgac cccgctgctg ctgcgtggcc tgaccggctc cgctcgtcgt
      961 ctgccagttc cgcgtgcgaa aatccattcc ctggactaca aagaccatga cggtgattat
     1021 aaagatcatg acatcgatta caaggatgac gatgacaaga tggacatcgc ggatctgcgt
     1081 accctgggtt acagccagca gcagcaggag aagatcaagc cgaaggtgcg cagcaccgtg
     1141 gctcagcacc acgaagccct ggtgggccac ggtttcaccc acgctcacat cgtggccctg
     1201 agccagcacc cagccgcgct gggcaccgtg gccgtgaaat atcaggacat gattgctgcc
     1261 ctgccagagg ccacccatga agctattgtg ggcgtgggca agcagtggag cggtgctcgt
     1321 gcgctggagg cgctgctgac cgtggctggt gaactgcgtg gtccgccgct gcagctggat
     1381 accggtcagc tgctgaaaat tgccaaacgt ggcggtgtga ccgcggtgga agccgtgcat
     1441 gcttggcgta atgctctgac cggtgcgccg ctgaacttaa cgccggaaca agttgttgcc
     1501 atcgccagca acaacggcgg caagcaagct ctggaaaccg tgcaggccct gcttccagtc
     1561 ctgtgtcagg cccacggcct gacccctcag caggtggtcg ccatcgcctc taacatcggc
     1621 ggaaagcagg ctctggagac cgtgcagcgg ctgctgcccg tgctgtgcca ggcccacggc
     1681 ctgacacctc agcaggtggt ggccattgct agcaacatcg gaggcaagca ggccctggaa
     1741 accgtgcaaa gactgctgcc cgtgctgtgc caggcccacg gcctcacacc cgagcaagtg
     1801 gtggccatcg ctagcaacaa cggcggcaag caggctctcg agacagtcca agctctgctg
     1861 ccagtgctgt gccaagctca cggcctgaca cccgagcagg tggtcgccat cgcctcccac
     1921 gatggcggca aacaggctct ggaaacagtg caggccctgc tgcctgtgct gtgccaggcc
     1981 cacggcctga cccctgagca ggtggtggcc atcgccagca atggcggagg caagcaggcc
     2041 ctggaaaccg tgcaggctct gctgcctgtg ctgtgtcaag cccatggcct gacccctgag
     2101 caggtggtgg ctatcgcctc taatggcgga ggcaagcagg ccctggaaac cgtgcagaga
     2161 ctgctgcctg tgctgtgcca ggcccatgga ctgacccctg agcaggttgt tgcaatcgcc
     2221 agccacgacg gcggcaaaca agccctggaa accgtgcagg ccctgctgcc cgtgctgtgc
     2281 caggcccacg gactgacccc acagcaggtt gtggccatcg ccagcaacat cggcggcaag
     2341 caggccctgg aaacagtgca gcggctgctg cctgtgctgt gtcaggccca cggcctgacc
     2401 cctcaacagg tggtggccat cgccagccac gacggcggca agcaggctct tgagaccgtg
     2461 cagagactgc tgcccgtgct gtgccaggcc cacggactga cacctcagca ggtggtggcc
     2521 atcgcctccc acgacggcgg gagacctgcc ctcgaaagca ttgtggctca gctgagccgt
     2581 ccagacccgg cgctggcggc tctgaccaac gatcacctgg tggcgctggc ttgcctgggc
     2641 ggtcgtccgg ccctggatgc cgtgaagaaa ggcctgggtg gatccggcag ctacgccctg
     2701 ggtccgtatc agattagcgc cccgcagctg ccagcttaca atggtcagac cgtgggtacc
     2761 ttctactatg tgaacgacgc gggcggtctg gagagcaagg tgtttagcag cggcggtcca
     2821 accccgtacc caaactatgc caatgccggt catgtggccg gtcagagcgc cctgttcatg
     2881 cgtgataacg gcatcagcga gggtctggtg ttccacaaca acccggaagg cacctgcggt
     2941 ttttgcgtga acatgaccga gaccctgctg ccggaaaacg cgaaaatgac cgtggtgccg
     3001 ccggaaggtt ctggcggctc aactaatctg agcgacatca ttgagaagga gactgggaaa
     3061 cagctggtca ttcaggagtc catcctgatg ctgcctgagg aggtggagga agtgatcggc
     3121 aacaagccag agtctgacat cctggtgcac accgcctacg acgagtccac agatgagaat
     3181 gtgatgctgc tgacctctga cgcccccgag tataagcctt gggccctggt catccaggat
     3241 tctaacggcg agaataagat caagatgctg ggaagcggag ctactaactt cagcctgctg
     3301 aagcaggctg gagacgtgga ggagaaccct ggacctatgg tgtccaaggg cgaggaagat
     3361 aacatggcca tcatcaagga gttcatgagg tttaaggtcc acatggaggg ttcagtcaat
     3421 ggccacgagt tcgagattga aggcgagggc gagggccgcc cctacgaagg gacacagacg
     3481 gcgaaattga aggtgaccaa aggcgggcca ttgcccttcg catgggacat cttgtcccct
     3541 cagtttatgt atggcagcaa ggcctatgtt aagcaccccg ctgatatccc ggactacttg
     3601 aagctgtcct ttccagaggg gtttaaatgg gagcgcgtta tgaatttcga agacggagga
     3661 gtggttacgg tgacgcagga ctcatccctg caggacggag aatttatata taaggttaag
     3721 ttgagaggca caaacttccc aagcgacggc cctgtgatgc agaagaaaac aatggggtgg
     3781 gaagcttcca gcgagcgcat gtaccccgaa gatggcgccc tcaagggcga gataaagcaa
     3841 aggctgaaac ttaaggacgg cggtcattac gacgcggagg tcaagacaac ttacaaggct
     3901 aaaaaacccg ttcagttgcc tggggcttac aatgttaata tcaaacttga catcacaagc
     3961 cacaatgaag actatacgat cgtggagcag tatgaacgag cggaaggcag gcactcaacg
     4021 ggggggatgg acgagcttta caagtgataa ggggtctttg tcctctgtac tgtctctctc
     4081 cttgccccta acccaaaaag cttcattttt ctgtgtaggc tgcacaagag ccttgattga
     4141 agatatattc tttctgaaca gtatttaagg tttccaataa aatgtacacc cctcagaact
     4201 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     4261 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4321 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4381 cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc
     4441 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4501 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4561 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt
     4621 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     4681 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg
     4741 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4801 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     4861 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4921 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     4981 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     5041 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     5101 tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa gaagaatcca
     5161 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     5221 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     5281 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     5341 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     5401 gacggtgtcg acaggtgctt ctcgatctgc atcctgggat caaagcgata gtgaaggaca
     5461 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     5521 agggctaagc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     5581 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5641 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     5701 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5761 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     5821 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5881 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5941 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6001 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6061 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6121 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6181 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6241 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6421 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     6481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6661 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     6721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6841 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6961 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7021 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7081 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7141 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7201 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7261 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7321 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7381 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7441 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7501 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7561 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7621 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7681 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7741 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7801 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7861 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7921 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7981 cgcacatttc cccgaaaagt gccacctgac gtc
//
