LOCUS       Exported                7185 bp DNA     circular SYN 30-NOV-2021
DEFINITION  mito 143b, T1413I SOD2 MTS_3xHA_11696  Right-TALE B_2aa_DddE(149-178
            )_1xUGI_SOD2 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Guilinger JP Pattanayak V Reyon D Tsai SQ Sander JD Joung JK Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58 
            http://www.snapgene.com
REFERENCE   3  (bases 1 to 7185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Exported 
            Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com"
COMMENT     Imported From
            Path: /Users/bmok/Downloads
            Filename: addgene-plasmid-51444-sequence-78455.gbk
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="synthetic DNA construct"
                     /mol_type="genomic DNA"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="color: black; sequence: GCGGCATCAGAGCAGATTGT"
     primer_bind     198..213
                     /label=BM719
                     /note="color: black; sequence: GCGCTGCTTCGCGATG"
     primer_bind     209..229
                     /label=BM525
                     /note="color: black; sequence: CGATGTACGGGCCAGATATAC"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     365..379
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     complement(419..435)
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     687..706
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     complement(687..704)
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     770..790
                     /label=CMV-F
                     /note="color: black; sequence: GCAAATGGGCGGTAGGCGTGT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     864..883
                     /label=T7
                     /note="color: black; sequence: AATACGACTCACTATAGGGA"
     primer_bind     complement(888..913)
                     /label=BM858
                     /note="color: black; sequence: ACAGGGCCATGGTGCTAGCCAGCTTG"
     primer_bind     901..926
                     /label=BM856
                     /note="color: black; sequence: ACCATGGCCCTGTCCCGTGCGGTTTG"
     CDS             904..996
                     /label=SOD2-MTS
                     /note="/created by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    904..906
                     
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_difference 907..909
                     /label=not in SOD2 MTS gene block
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    910..926
                     /label=anneal
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(982..1005)
                     /label=BM857
                     /note="color: black; sequence: ATACGGGTAGTCCGGCAGGGAGTG"
     primer_bind     995..1015
                     /label=BM859
                     /note="color: black; sequence: ACTACCCGTATGATGTTCCGG"
     CDS             997..1083
                     
                     /note="/created by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(1036..1053)
                     /label=BM860
                     /note="color: black; sequence: ATGTCCATAGCGTAGTCTGGGACGTC"
     primer_bind     complement(1066..1091)
                     /label=BM860
                     /note="color: black; sequence: ATGTCCATAGCGTAGTCTGGGACGTC"
     misc_feature    complement(1066..1083)
                     /label=annealing
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     1079..1103
                     /label=BM861
                     /note="color: black; sequence: ACGCTATGGACATCGCGGATCTGCG"
     CDS             complement(1087..1500)
                     
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: LEFT"
     primer_bind     1465..1484
                     /label=BM890
                     /note="color: black; sequence: GCTTGGCGTAATGCTCTGAC"
     primer_bind     complement(1470..1488)
                     /label=BM1529
                     /note="color: black; sequence: ACCGGTCAGAGCATTACGC"
     primer_bind     1475..1541
                     /label=BM1893
                     /note="color: black; sequence: 
                     ATGCTCTGACCGGTGCGCCGCTGAACTTAACGCCGGAACAAGTTGTTGCCATCGCCAGC
                     AACAACGG"
     CDS             1501..2682
                     /label=m.11696b Right TALE
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1503
                     /label=G>C
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1506
                     /label=C>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1509
                     /label=T>C
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1512
                     /label=G>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1513..1532
                     
                     /note="Geneious type: motif "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     1726..1745
                     /label=BM1893
                     /note="color: black; sequence: 
                     ATGCTCTGACCGGTGCGCCGCTGAACTTAACGCCGGAACAAGTTGTTGCCATCGCCAGC
                     AACAACGG"
     primer_bind     2338..2357
                     /label=BM1893
                     /note="color: black; sequence: 
                     ATGCTCTGACCGGTGCGCCGCTGAACTTAACGCCGGAACAAGTTGTTGCCATCGCCAGC
                     AACAACGG"
     misc_feature    2682
                     /label=G<A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2683..2805
                     /label=C terminal canonical (from mito 18, m.14459 left)
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     2776..2793
                     /label=BM892
                     /note="color: black; sequence: GCCCTGGATGCGGTGAAG"
     primer_bind     complement(2788..2809)
                     /label=BM905
                     /note="color: black; sequence: ATCCACCCAGGCCTTTCTTCAC"
     primer_bind     2806..2828
                     /label=annealing region of BM 909
                     /note="color: black; sequence: GGATCCGCCATTCCAGTGAAGCG"
     CDS             2812..2901
                     /label=DddE(149-178(
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(2833..2858)
                     /label=BM1880
                     /note="color: black; sequence: ATAAACACTTTGGTTTTACCGGTAGC"
     primer_bind     2850..2876
                     /label=BM1879
                     /note="color: black; sequence: AGTGTTTATCGGTAACAGCAACAGCCC"
     misc_feature    2857..2859
                     /label=T1413I
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2886..2930)
                     /label=BM1027
                     /note="color: black; sequence: 
                     ATGTCGCTCAGATTAGTTGAGCCGCCAGAGCAACCGCCTTTGGTC"
     primer_bind     2913..2935
                     /label=BM941
                     /note="color: black; sequence: AACTAATCTGAGCGACATCATTG"
     misc_difference 2914..3162
                     
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2914..3162
                     /label=UGI
                     /note="/created by=kzhao"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(3140..3166)
                     /label=BM1032
                     /note="color: black; sequence: ATCACAGCATCTTGATCTTATTCTCGC"
     3'UTR           3169..3370
                     /label=SOD2-3'UTR
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3345..3349
                     
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     rep_origin      3416..3844
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow  
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(3504..3523)
                     /label=F1ori-R
                     /note="color: black; sequence: AAGGGAAGAAAGCGAAAGGA"
     primer_bind     3714..3735
                     /label=F1ori-F
                     /note="color: black; sequence: TGGACTCTTGTTCCAAACTGGA"
     primer_bind     complement(3854..3874)
                     /label=pBABE 3'
                     /note="color: black; sequence: CACCCTAACTGACACACATTC"
     promoter        3858..4188
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4039..4174
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     4102..4121
                     /label=SV40pro-F
                     /note="color: black; sequence: ATTTATGCAGAGGCCGAGGC"
     primer_bind     complement(4123..4135)
                     /label=SV40pro-F
                     /note="color: black; sequence: ATTTATGCAGAGGCCGAGGC"
     promoter        4236..4283
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4302..4700
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #993366"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4858..4979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(4896..4915)
                     /label=SV40pA-R
                     /note="color: black; sequence: TGAAATTTGTGATGCTATTG"
     primer_bind     4950..4969
                     /label=EBV-rev
                     /note="color: black; sequence: TGGTTTGTCCAAACTCATCA"
     primer_bind     complement(5029..5045)
                     /label=M13 rev
                     /note="color: black; sequence: ACAGGAAACAGCTATGA"
     primer_bind     complement(5029..5045)
                     /label=M13 Reverse
                     /note="color: black; sequence: ACAGGAAACAGCTATGA"
     primer_bind     complement(5042..5064)
                     /label=M13/pUC Reverse
                     /note="color: black; sequence: GAGCGGATAACAATTTCACACAG"
     protein_bind    5052..5068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to  
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b; direction: RIGHT"
     promoter        complement(5076..5106)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    5121..5142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     complement(5153..5169)
                     
                     /note="color: black; sequence: GAAAGCGGGCAGTGAGC"
     primer_bind     complement(5260..5277)
                     /label=L4440
                     /note="color: black; sequence: CAGCGAGTCAGTGAGCGA"
     rep_origin      complement(5430..6018)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(5511..5530)
                     /label=pBR322ori-F
                     /note="color: black; sequence: GGGGAAACGCCTGGTATCTT"
     CDS             complement(6189..7049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6813..6832
                     /label=Amp-R
                     /note="color: black; sequence: TAATACCGCGCCACATAGCA"
     promoter        complement(7050..7154)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 accatggccc tgtcccgtgc ggtttgtggc acctcccgtc aactggctcc ggttctgggt
      961 tatctgggtt cccgtcaaaa acactccctg ccggactacc cgtatgatgt tccggattac
     1021 gctggctacc catacgacgt cccagactac gctggctacc catacgacgt cccagactac
     1081 gctatggaca tcgcggatct gcgtaccctg ggttacagcc agcagcagca ggagaagatc
     1141 aagccgaagg tgcgcagcac cgtggctcag caccacgaag ccctggtggg ccacggtttc
     1201 acccacgctc acattgtggc cctgagccag cacccagccg cgctgggcac cgtggccgtg
     1261 aaatatcagg atatgattgc tgccctgcca gaggccaccc atgaagctat tgtgggcgtg
     1321 ggcaagcagt ggagcggtgc tcgtgcgctg gaggcgctgc tgaccgtggc tggtgaactg
     1381 cgtggtccgc cgctgcagct ggacaccggt cagctgctga aaatcgcgaa acgtggcggt
     1441 gtgaccgcgg tggaagccgt gcatgcttgg cgtaatgctc tgaccggtgc gccgctgaac
     1501 ctcacacccg aacaggttgt tgctatcgcc agcaacaacg gaggaaagca ggcccttgag
     1561 acagtgcagg ccctgctccc cgtgctgtgc caggcccacg gactgacccc tcaacaggtg
     1621 gttgctatcg cctccaacaa cggcggcaag caggccctgg aaaccgtgca gaggctgctg
     1681 cccgtgctgt gtcaggccca cggcctgacc cctcagcagg tcgtggccat cgccagcaac
     1741 aacggcggaa agcaggcact tgaaaccgtc caaagactgc tgcccgtgct gtgccaggcc
     1801 catggactga cacccgagca ggtggtggcc attgccagcc acgacggcgg aaagcaagct
     1861 ctggagaccg ttcaagctct gctgccagtg ctgtgccagg cccacggact gacaccagag
     1921 caggtggtgg ctatcgcctc taacaacggc ggcaagcagg cccttgaaac cgtgcaggct
     1981 ctgctgcctg tgctgtgcca ggctcacggc ctgacacctg agcaggtcgt ggccatcgcc
     2041 tctaatatcg gcggcaagca ggccctggag acagtgcagg ctctgctgcc tgttctgtgt
     2101 caggcccacg gcctgacccc tgagcaggtg gtggccatcg ccagcaacgg cggcggcaag
     2161 caggccctcg agacagtgca gagactgctg cccgtgctgt gccaggccca cggcctgaca
     2221 cctgagcagg tggtggctat cgccagcaac ggcggcggca aacaagccct ggaaacagtg
     2281 caagctcttc tgcctgtgct gtgtcaggcc cacggactga cccctcagca ggtggtggcc
     2341 atcgccagca acatcggcgg taagcaggcc ctggaaaccg tgcagcggct gctgcctgtg
     2401 ctgtgccagg cccacggcct gacccctgag caagtggtgg ccatcgctag caatggaggc
     2461 ggcaaacagg ccctggaaac cgtgcagcgg ctgctgcctg tgctgtgcca ggcccacggc
     2521 ctgaccccac aacaagtggt ggccatcgcc agcaacaatg gcggcaaaca ggccctggaa
     2581 accgtgcaga gactgctgcc tgtgctgtgc caggcccacg gcctgacccc ccagcaggtg
     2641 gtggccatcg cctccaatat cggcgggaga cccgccctgg aaagcattgt ggctcagctg
     2701 agccgtccag acccggccct ggcggctctg accaacgatc acctggtggc gctggcttgc
     2761 ctgggcggtc gtccggccct ggatgcggtg aagaaaggcc tgggtggatc cgccattcca
     2821 gtgaagcgcg gcgctaccgg tgaaaccaaa gtgtttatcg gtaacagcaa cagcccgaag
     2881 agcccgacca aaggcggttg ctctggcggc tcaactaatc tgagcgacat cattgagaag
     2941 gagactggga aacagctggt cattcaggag tccatcctga tgctgcctga ggaggtggag
     3001 gaagtgatcg gcaacaagcc agagtctgac atcctggtgc acaccgccta cgacgagtcc
     3061 acagatgaga atgtgatgct gctgacctct gacgcccccg agtataagcc ttgggccctg
     3121 gtcatccagg attctaacgg cgagaataag atcaagatgc tgtgataaac cacgatcgtt
     3181 atgctgatca taccctaatg atcccagcaa gataatgtcc tgtcttctaa gatgtgcatc
     3241 aagcctggta catactgaaa accctataag gtcctggata atttttgttt gattattcat
     3301 tgaagaaaca tttattttcc aattgtgtga agtttttgac tgttaataaa agaatctgtc
     3361 aaccatcaaa cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     3421 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3481 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3541 gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct
     3601 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3661 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3721 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3781 attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3841 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca
     3901 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     3961 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4021 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4081 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     4141 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4201 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     4261 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag cctttgtctc
     4321 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
     4381 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
     4441 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
     4501 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
     4561 tgagcccctg cggacggtgt cgacaggtgc ttctcgatct gcatcctggg atcaaagcga
     4621 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
     4681 gttatgtgtg ggagggctaa gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     4741 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4801 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     4861 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4921 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4981 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     5041 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5101 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     5161 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5221 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5281 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5341 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5401 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5461 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5521 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5581 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     5641 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5701 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5761 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5821 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     5881 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5941 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6001 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6061 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6121 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6181 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6241 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6301 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6361 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6421 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6481 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6541 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6601 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6661 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6721 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6781 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6841 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6901 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6961 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7021 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7081 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7141 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
