LOCUS       Exported                7914 bp DNA     circular SYN 30-NOV-2021
DEFINITION  mito143b-GFP SOD2 MTS_3xHA_11696  Right-TALE B_2aa_DddE(149-178 
            )_1xUGI_ATP5B 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Guilinger JP Pattanayak V Reyon D Tsai SQ Sander JD Joung JK Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58 
            http://www.snapgene.com
REFERENCE   3  (bases 1 to 7914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Exported 
            Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com"
COMMENT     Imported From
            Path: /Users/bmok/Downloads
            Filename: addgene-plasmid-51444-sequence-78455.gbk
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="synthetic DNA construct"
                     /mol_type="genomic DNA"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="color: black; sequence: GCGGCATCAGAGCAGATTGT"
     primer_bind     198..213
                     /label=BM719
                     /note="color: black; sequence: GCGCTGCTTCGCGATG"
     primer_bind     209..229
                     /label=BM525
                     /note="color: black; sequence: CGATGTACGGGCCAGATATAC"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     365..379
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     complement(419..435)
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     687..706
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     complement(687..704)
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     770..790
                     /label=CMV-F
                     /note="color: black; sequence: GCAAATGGGCGGTAGGCGTGT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     864..883
                     /label=T7
                     /note="color: black; sequence: AATACGACTCACTATAGGGA"
     primer_bind     complement(888..913)
                     /label=BM858
                     /note="color: black; sequence: ACAGGGCCATGGTGCTAGCCAGCTTG"
     primer_bind     901..926
                     /label=BM856
                     /note="color: black; sequence: ACCATGGCCCTGTCCCGTGCGGTTTG"
     CDS             904..996
                     /label=SOD2-MTS
                     /note="/created by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    904..906
                     
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_difference 907..909
                     /label=not in SOD2 MTS gene block
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    910..926
                     /label=anneal
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(982..1005)
                     /label=BM857
                     /note="color: black; sequence: ATACGGGTAGTCCGGCAGGGAGTG"
     primer_bind     995..1015
                     /label=BM859
                     /note="color: black; sequence: ACTACCCGTATGATGTTCCGG"
     CDS             997..1083
                     
                     /note="/created by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(1036..1053)
                     /label=BM860
                     /note="color: black; sequence: ATGTCCATAGCGTAGTCTGGGACGTC"
     primer_bind     complement(1066..1091)
                     /label=BM860
                     /note="color: black; sequence: ATGTCCATAGCGTAGTCTGGGACGTC"
     misc_feature    complement(1066..1083)
                     /label=annealing
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     1079..1103
                     /label=BM861
                     /note="color: black; sequence: ACGCTATGGACATCGCGGATCTGCG"
     CDS             complement(1087..1500)
                     
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: LEFT"
     primer_bind     1465..1484
                     /label=BM890
                     /note="color: black; sequence: GCTTGGCGTAATGCTCTGAC"
     primer_bind     complement(1470..1488)
                     /label=BM1529
                     /note="color: black; sequence: ACCGGTCAGAGCATTACGC"
     primer_bind     1475..1541
                     /label=BM1893
                     /note="color: black; sequence: 
                     ATGCTCTGACCGGTGCGCCGCTGAACTTAACGCCGGAACAAGTTGTTGCCATCGCCAGC
                     AACAACGG"
     CDS             1501..2682
                     /label=m.11696b Right TALE
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1503
                     /label=G>C
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1506
                     /label=C>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1509
                     /label=T>C
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1512
                     /label=G>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1513..1532
                     
                     /note="Geneious type: motif "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     1726..1745
                     /label=BM1893
                     /note="color: black; sequence: 
                     ATGCTCTGACCGGTGCGCCGCTGAACTTAACGCCGGAACAAGTTGTTGCCATCGCCAGC
                     AACAACGG"
     primer_bind     2338..2357
                     /label=BM1893
                     /note="color: black; sequence: 
                     ATGCTCTGACCGGTGCGCCGCTGAACTTAACGCCGGAACAAGTTGTTGCCATCGCCAGC
                     AACAACGG"
     misc_feature    2682
                     /label=G<A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2683..2805
                     /label=C terminal canonical (from mito 18, m.14459 left)
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     2776..2793
                     /label=BM892
                     /note="color: black; sequence: GCCCTGGATGCGGTGAAG"
     primer_bind     complement(2788..2809)
                     /label=BM905
                     /note="color: black; sequence: ATCCACCCAGGCCTTTCTTCAC"
     primer_bind     2806..2828
                     /label=annealing region of BM 909
                     /note="color: black; sequence: GGATCCGCCATTCCAGTGAAGCG"
     CDS             2812..2901
                     /label=DddE(149-178(
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(2833..2858)
                     /label=BM1880
                     /note="color: black; sequence: ATAAACACTTTGGTTTTACCGGTAGC"
     primer_bind     2850..2876
                     /label=BM1879
                     /note="color: black; sequence: AGTGTTTATCGGTAACAGCAACAGCCC"
     misc_feature    2857..2859
                     /label=T1413I
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2886..2930)
                     /label=BM1027
                     /note="color: black; sequence: 
                     ATGTCGCTCAGATTAGTTGAGCCGCCAGAGCAACCGCCTTTGGTC"
     primer_bind     2913..2935
                     /label=BM941
                     /note="color: black; sequence: AACTAATCTGAGCGACATCATTG"
     misc_difference 2914..3162
                     
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2914..3162
                     /label=UGI
                     /note="/created by=kzhao"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3163..3945
                     /label=from pPC1349
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             3172..3228
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1  
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding 
                     separatepolypeptides."
                     /note="color: #993366"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3229..3945
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3275..3296)
                     /label=EGFP-N
                     /note="color: black; sequence: ACGTCGCCGTCCAGCTCGACCA"
     primer_bind     complement(3535..3554)
                     /label=BM2096
                     /note="color: black; sequence: GTCTTGTAGTTGCCGTCGTC"
     primer_bind     3714..3735
                     /label=PC1091
                     /note="color: black; sequence: CAAGGTGAACTTCAAGATCCGC"
     primer_bind     3883..3904
                     /label=EGFP-C
                     /note="color: black; sequence: ATGGTCCTGCTGGAGTTCGTGA"
     3'UTR           3952..4099
                     /label=ATP5B 3'UTR
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      4077..4081
                     
                     /note="/created by=bmok"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4145..4573
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow  
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(4233..4252)
                     /label=F1ori-R
                     /note="color: black; sequence: AAGGGAAGAAAGCGAAAGGA"
     primer_bind     4443..4464
                     /label=F1ori-F
                     /note="color: black; sequence: TGGACTCTTGTTCCAAACTGGA"
     primer_bind     complement(4583..4603)
                     /label=pBABE 3'
                     /note="color: black; sequence: CACCCTAACTGACACACATTC"
     promoter        4587..4917
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4768..4903
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     4831..4850
                     /label=SV40pro-F
                     /note="color: black; sequence: ATTTATGCAGAGGCCGAGGC"
     primer_bind     complement(4852..4864)
                     /label=SV40pro-F
                     /note="color: black; sequence: ATTTATGCAGAGGCCGAGGC"
     promoter        4965..5012
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5031..5429
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #993366"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5587..5708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(5625..5644)
                     /label=SV40pA-R
                     /note="color: black; sequence: TGAAATTTGTGATGCTATTG"
     primer_bind     5679..5698
                     /label=EBV-rev
                     /note="color: black; sequence: TGGTTTGTCCAAACTCATCA"
     primer_bind     complement(5758..5774)
                     /label=M13 rev
                     /note="color: black; sequence: ACAGGAAACAGCTATGA"
     primer_bind     complement(5758..5774)
                     /label=M13 Reverse
                     /note="color: black; sequence: ACAGGAAACAGCTATGA"
     primer_bind     complement(5771..5793)
                     /label=M13/pUC Reverse
                     /note="color: black; sequence: GAGCGGATAACAATTTCACACAG"
     protein_bind    5781..5797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to  
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b; direction: RIGHT"
     promoter        complement(5805..5835)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    5850..5871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     complement(5882..5898)
                     
                     /note="color: black; sequence: GAAAGCGGGCAGTGAGC"
     primer_bind     complement(5989..6006)
                     /label=L4440
                     /note="color: black; sequence: CAGCGAGTCAGTGAGCGA"
     rep_origin      complement(6159..6747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(6240..6259)
                     /label=pBR322ori-F
                     /note="color: black; sequence: GGGGAAACGCCTGGTATCTT"
     CDS             complement(6918..7778)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7542..7561
                     /label=Amp-R
                     /note="color: black; sequence: TAATACCGCGCCACATAGCA"
     promoter        complement(7779..7883)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 accatggccc tgtcccgtgc ggtttgtggc acctcccgtc aactggctcc ggttctgggt
      961 tatctgggtt cccgtcaaaa acactccctg ccggactacc cgtatgatgt tccggattac
     1021 gctggctacc catacgacgt cccagactac gctggctacc catacgacgt cccagactac
     1081 gctatggaca tcgcggatct gcgtaccctg ggttacagcc agcagcagca ggagaagatc
     1141 aagccgaagg tgcgcagcac cgtggctcag caccacgaag ccctggtggg ccacggtttc
     1201 acccacgctc acattgtggc cctgagccag cacccagccg cgctgggcac cgtggccgtg
     1261 aaatatcagg atatgattgc tgccctgcca gaggccaccc atgaagctat tgtgggcgtg
     1321 ggcaagcagt ggagcggtgc tcgtgcgctg gaggcgctgc tgaccgtggc tggtgaactg
     1381 cgtggtccgc cgctgcagct ggacaccggt cagctgctga aaatcgcgaa acgtggcggt
     1441 gtgaccgcgg tggaagccgt gcatgcttgg cgtaatgctc tgaccggtgc gccgctgaac
     1501 ctcacacccg aacaggttgt tgctatcgcc agcaacaacg gaggaaagca ggcccttgag
     1561 acagtgcagg ccctgctccc cgtgctgtgc caggcccacg gactgacccc tcaacaggtg
     1621 gttgctatcg cctccaacaa cggcggcaag caggccctgg aaaccgtgca gaggctgctg
     1681 cccgtgctgt gtcaggccca cggcctgacc cctcagcagg tcgtggccat cgccagcaac
     1741 aacggcggaa agcaggcact tgaaaccgtc caaagactgc tgcccgtgct gtgccaggcc
     1801 catggactga cacccgagca ggtggtggcc attgccagcc acgacggcgg aaagcaagct
     1861 ctggagaccg ttcaagctct gctgccagtg ctgtgccagg cccacggact gacaccagag
     1921 caggtggtgg ctatcgcctc taacaacggc ggcaagcagg cccttgaaac cgtgcaggct
     1981 ctgctgcctg tgctgtgcca ggctcacggc ctgacacctg agcaggtcgt ggccatcgcc
     2041 tctaatatcg gcggcaagca ggccctggag acagtgcagg ctctgctgcc tgttctgtgt
     2101 caggcccacg gcctgacccc tgagcaggtg gtggccatcg ccagcaacgg cggcggcaag
     2161 caggccctcg agacagtgca gagactgctg cccgtgctgt gccaggccca cggcctgaca
     2221 cctgagcagg tggtggctat cgccagcaac ggcggcggca aacaagccct ggaaacagtg
     2281 caagctcttc tgcctgtgct gtgtcaggcc cacggactga cccctcagca ggtggtggcc
     2341 atcgccagca acatcggcgg taagcaggcc ctggaaaccg tgcagcggct gctgcctgtg
     2401 ctgtgccagg cccacggcct gacccctgag caagtggtgg ccatcgctag caatggaggc
     2461 ggcaaacagg ccctggaaac cgtgcagcgg ctgctgcctg tgctgtgcca ggcccacggc
     2521 ctgaccccac aacaagtggt ggccatcgcc agcaacaatg gcggcaaaca ggccctggaa
     2581 accgtgcaga gactgctgcc tgtgctgtgc caggcccacg gcctgacccc ccagcaggtg
     2641 gtggccatcg cctccaatat cggcgggaga cccgccctgg aaagcattgt ggctcagctg
     2701 agccgtccag acccggccct ggcggctctg accaacgatc acctggtggc gctggcttgc
     2761 ctgggcggtc gtccggccct ggatgcggtg aagaaaggcc tgggtggatc cgccattcca
     2821 gtgaagcgcg gcgctaccgg tgaaaccaaa gtgtttatcg gtaacagcaa cagcccgaag
     2881 agcccgacca aaggcggttg ctctggcggc tcaactaatc tgagcgacat cattgagaag
     2941 gagactggga aacagctggt cattcaggag tccatcctga tgctgcctga ggaggtggag
     3001 gaagtgatcg gcaacaagcc agagtctgac atcctggtgc acaccgccta cgacgagtcc
     3061 acagatgaga atgtgatgct gctgacctct gacgcccccg agtataagcc ttgggccctg
     3121 gtcatccagg attctaacgg cgagaataag atcaagatgc tgggaagcgg agctactaac
     3181 ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctat ggtgagcaag
     3241 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3301 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     3361 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3421 ctgacctatg gagtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     3481 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     3541 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     3601 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3661 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     3721 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     3781 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3841 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3901 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtgata aggggtcttt
     3961 gtcctctgta ctgtctctct ccttgcccct aacccaaaaa gcttcatttt tctgtgtagg
     4021 ctgcacaaga gccttgattg aagatatatt ctttctgaac agtatttaag gtttccaata
     4081 aaatgtacac ccctcagaac ttctgaggcg gaaagaacca gctggggctc tagggggtat
     4141 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4201 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4261 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga
     4321 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4381 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4441 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4501 ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4561 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     4621 ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4681 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4741 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4801 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     4861 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4921 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     4981 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagc
     5041 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     5101 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     5161 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     5221 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     5281 ggggcatctt gagcccctgc ggacggtgtc gacaggtgct tctcgatctg catcctggga
     5341 tcaaagcgat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     5401 tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca ggactgacac
     5461 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5521 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     5581 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5641 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5701 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     5761 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5821 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     5881 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     5941 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6001 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6061 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     6361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     6601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6901 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6961 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7021 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7081 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7141 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7201 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7261 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7321 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7381 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7441 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7501 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7561 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7621 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7681 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7741 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7801 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7861 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
