LOCUS       Exported                7107 bp DNA     circular SYN 30-NOV-2021
DEFINITION  mito143c, wildtype COX8a MTS_3xFLAG_11696  Right-TALE 
            B_2aa_DddE(149-178 )_1xUGI_ATP5b 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Guilinger JP Pattanayak V Reyon D Tsai SQ Sander JD Joung JK Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58 
            http://www.snapgene.com
REFERENCE   3  (bases 1 to 7107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Exported 
            Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com"
COMMENT     Imported From
            Path: /Users/bmok/Downloads
            Filename: addgene-plasmid-51444-sequence-78455.gbk
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="synthetic DNA construct"
                     /lab_host="synthetic DNA construct"
                     /mol_type="other DNA"
     source          907..909
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          907..909
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=From pHR_087
                     /note="/modified by=hrees"
                     /note="color: #ffffff"
     source          910..993
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          910..993
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          994..1059
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          994..1059
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          3145..3292
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="color: black; sequence: GCGGCATCAGAGCAGATTGT"
     primer_bind     198..213
                     /label=BM719
                     /note="color: black; sequence: GCGCTGCTTCGCGATG"
     primer_bind     209..229
                     /label=BM525
                     /note="color: black; sequence: CGATGTACGGGCCAGATATAC"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     365..379
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     complement(419..435)
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     687..706
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     complement(687..704)
                     /label=BM720
                     /note="color: black; sequence: CCCATTGACGTCAATGGGAG"
     primer_bind     770..790
                     /label=CMV-F
                     /note="color: black; sequence: GCAAATGGGCGGTAGGCGTGT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     864..883
                     /label=T7
                     /note="color: black; sequence: AATACGACTCACTATAGGGA"
     primer_bind     complement(888..909)
                     /label=BM858
                     /note="color: black; sequence: ACAGGGCCATGGTGCTAGCCAGCTTG"
     misc_feature    904..906
                     
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             910..993
                     
                     /note="/created by=bmok"
                     /note="color: #993366; direction: RIGHT"
     CDS             994..1059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #993366"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1063..1476
                     
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     1441..1460
                     /label=BM890
                     /note="color: black; sequence: GCTTGGCGTAATGCTCTGAC"
     primer_bind     complement(1446..1464)
                     /label=BM1529
                     /note="color: black; sequence: ACCGGTCAGAGCATTACGC"
     primer_bind     1451..1517
                     /label=BM1893
                     /note="color: black; sequence: 
                     ATGCTCTGACCGGTGCGCCGCTGAACTTAACGCCGGAACAAGTTGTTGCCATCGCCAGC
                     AACAACGG"
     CDS             1477..2658
                     /label=m.11696b Right TALE
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1479
                     /label=G>C
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1482
                     /label=C>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1485
                     /label=T>C
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1488
                     /label=G>A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    1489..1508
                     
                     /note="Geneious type: motif "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     1702..1721
                     /label=BM1893
                     /note="color: black; sequence: 
                     ATGCTCTGACCGGTGCGCCGCTGAACTTAACGCCGGAACAAGTTGTTGCCATCGCCAGC
                     AACAACGG"
     primer_bind     2314..2333
                     /label=BM1893
                     /note="color: black; sequence: 
                     ATGCTCTGACCGGTGCGCCGCTGAACTTAACGCCGGAACAAGTTGTTGCCATCGCCAGC
                     AACAACGG"
     misc_feature    2658
                     /label=G<A
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2659..2781
                     /label=C terminal canonical (from mito 18, m.14459 left)
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     2752..2769
                     /label=BM892
                     /note="color: black; sequence: GCCCTGGATGCGGTGAAG"
     primer_bind     complement(2764..2785)
                     /label=BM905
                     /note="color: black; sequence: ATCCACCCAGGCCTTTCTTCAC"
     primer_bind     2782..2804
                     /label=annealing region of BM 909
                     /note="color: black; sequence: GGATCCGCCATTCCAGTGAAGCG"
     CDS             2788..2877
                     /label=DddE(149-178(
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(2809..2833)
                     /label=BM1880
                     /note="color: black; sequence: ATAAACACTTTGGTTTTACCGGTAGC"
     primer_bind     2826..2852
                     /label=BM1879
                     /note="color: black; sequence: AGTGTTTATCGGTAACAGCAACAGCCC"
     primer_bind     complement(2862..2906)
                     /label=BM1027
                     /note="color: black; sequence: 
                     ATGTCGCTCAGATTAGTTGAGCCGCCAGAGCAACCGCCTTTGGTC"
     primer_bind     2889..2911
                     /label=BM941
                     /note="color: black; sequence: AACTAATCTGAGCGACATCATTG"
     misc_difference 2890..3138
                     
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2890..3138
                     /label=UGI
                     /note="/created by=kzhao"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(3116..3142)
                     /label=BM1032
                     /note="color: black; sequence: ATCACAGCATCTTGATCTTATTCTCGC"
     3'UTR           3145..3292
                     /label=ATP5B 3'UTR
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      3270..3274
                     
                     /note="/created by=bmok"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      3338..3766
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow  
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(3426..3445)
                     /label=F1ori-R
                     /note="color: black; sequence: AAGGGAAGAAAGCGAAAGGA"
     primer_bind     3636..3657
                     /label=F1ori-F
                     /note="color: black; sequence: TGGACTCTTGTTCCAAACTGGA"
     primer_bind     complement(3776..3796)
                     /label=pBABE 3'
                     /note="color: black; sequence: CACCCTAACTGACACACATTC"
     promoter        3780..4110
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      3961..4096
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     4024..4043
                     /label=SV40pro-F
                     /note="color: black; sequence: ATTTATGCAGAGGCCGAGGC"
     primer_bind     complement(4045..4057)
                     /label=SV40pro-F
                     /note="color: black; sequence: ATTTATGCAGAGGCCGAGGC"
     promoter        4158..4205
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4224..4622
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #993366"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4780..4901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(4818..4837)
                     /label=SV40pA-R
                     /note="color: black; sequence: TGAAATTTGTGATGCTATTG"
     primer_bind     4872..4891
                     /label=EBV-rev
                     /note="color: black; sequence: TGGTTTGTCCAAACTCATCA"
     primer_bind     complement(4951..4967)
                     /label=M13 rev
                     /note="color: black; sequence: ACAGGAAACAGCTATGA"
     primer_bind     complement(4951..4967)
                     /label=M13 Reverse
                     /note="color: black; sequence: ACAGGAAACAGCTATGA"
     primer_bind     complement(4964..4986)
                     /label=M13/pUC Reverse
                     /note="color: black; sequence: GAGCGGATAACAATTTCACACAG"
     protein_bind    4974..4990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to  
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b; direction: RIGHT"
     promoter        complement(4998..5028)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    5043..5064
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     complement(5075..5091)
                     
                     /note="color: black; sequence: GAAAGCGGGCAGTGAGC"
     primer_bind     complement(5182..5199)
                     /label=L4440
                     /note="color: black; sequence: CAGCGAGTCAGTGAGCGA"
     rep_origin      complement(5352..5940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(5433..5452)
                     /label=pBR322ori-F
                     /note="color: black; sequence: GGGGAAACGCCTGGTATCTT"
     CDS             complement(6111..6971)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6735..6754
                     /label=Amp-R
                     /note="color: black; sequence: TAATACCGCGCCACATAGCA"
     promoter        complement(6972..7076)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 accatggcct ccgttctgac cccgctgctg ctgcgtggcc tgaccggctc cgctcgtcgt
      961 ctgccagttc cgcgtgcgaa aatccattcc ctggactaca aagaccatga cggtgattat
     1021 aaagatcatg acatcgatta caaggatgac gatgacaaga tggacatcgc ggatctgcgt
     1081 accctgggtt acagccagca gcagcaggag aagatcaagc cgaaggtgcg cagcaccgtg
     1141 gctcagcacc acgaagccct ggtgggccac ggtttcaccc acgctcacat cgtggccctg
     1201 agccagcacc cagccgcgct gggcaccgtg gccgtgaaat atcaggacat gattgctgcc
     1261 ctgccagagg ccacccatga agctattgtg ggcgtgggca agcagtggag cggtgctcgt
     1321 gcgctggagg cgctgctgac cgtggctggt gaactgcgtg gtccgccgct gcagctggat
     1381 accggtcagc tgctgaaaat tgccaaacgt ggcggtgtga ccgcggtgga agccgtgcat
     1441 gcttggcgta atgctctgac cggtgcgccg ctgaacctca cacccgaaca ggttgttgct
     1501 atcgccagca acaacggagg aaagcaggcc cttgagacag tgcaggccct gctccccgtg
     1561 ctgtgccagg cccacggact gacccctcaa caggtggttg ctatcgcctc caacaacggc
     1621 ggcaagcagg ccctggaaac cgtgcagagg ctgctgcccg tgctgtgtca ggcccacggc
     1681 ctgacccctc agcaggtcgt ggccatcgcc agcaacaacg gcggaaagca ggcacttgaa
     1741 accgtccaaa gactgctgcc cgtgctgtgc caggcccatg gactgacacc cgagcaggtg
     1801 gtggccattg ccagccacga cggcggaaag caagctctgg agaccgttca agctctgctg
     1861 ccagtgctgt gccaggccca cggactgaca ccagagcagg tggtggctat cgcctctaac
     1921 aacggcggca agcaggccct tgaaaccgtg caggctctgc tgcctgtgct gtgccaggct
     1981 cacggcctga cacctgagca ggtcgtggcc atcgcctcta atatcggcgg caagcaggcc
     2041 ctggagacag tgcaggctct gctgcctgtt ctgtgtcagg cccacggcct gacccctgag
     2101 caggtggtgg ccatcgccag caacggcggc ggcaagcagg ccctcgagac agtgcagaga
     2161 ctgctgcccg tgctgtgcca ggcccacggc ctgacacctg agcaggtggt ggctatcgcc
     2221 agcaacggcg gcggcaaaca agccctggaa acagtgcaag ctcttctgcc tgtgctgtgt
     2281 caggcccacg gactgacccc tcagcaggtg gtggccatcg ccagcaacat cggcggtaag
     2341 caggccctgg aaaccgtgca gcggctgctg cctgtgctgt gccaggccca cggcctgacc
     2401 cctgagcaag tggtggccat cgctagcaat ggaggcggca aacaggccct ggaaaccgtg
     2461 cagcggctgc tgcctgtgct gtgccaggcc cacggcctga ccccacaaca agtggtggcc
     2521 atcgccagca acaatggcgg caaacaggcc ctggaaaccg tgcagagact gctgcctgtg
     2581 ctgtgccagg cccacggcct gaccccccag caggtggtgg ccatcgcctc caatatcggc
     2641 gggagacccg ccctggaaag cattgtggct cagctgagcc gtccagaccc ggcgctggcg
     2701 gctctgacca acgatcacct ggtggcgctg gcttgcctgg gcggtcgtcc ggccctggat
     2761 gccgtgaaga aaggcctggg tggatccgcc attccagtga agcgcggcgc taccggtgaa
     2821 accaaagtgt ttaccggtaa cagcaacagc ccgaagagcc cgaccaaagg cggttgctct
     2881 ggcggctcaa ctaatctgag cgacatcatt gagaaggaga ctgggaaaca gctggtcatt
     2941 caggagtcca tcctgatgct gcctgaggag gtggaggaag tgatcggcaa caagccagag
     3001 tctgacatcc tggtgcacac cgcctacgac gagtccacag atgagaatgt gatgctgctg
     3061 acctctgacg cccccgagta taagccttgg gccctggtca tccaggattc taacggcgag
     3121 aataagatca agatgctgtg ataaggggtc tttgtcctct gtactgtctc tctccttgcc
     3181 cctaacccaa aaagcttcat ttttctgtgt aggctgcaca agagccttga ttgaagatat
     3241 attctttctg aacagtattt aaggtttcca ataaaatgta cacccctcag aacttctgag
     3301 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     3361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3481 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3541 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3661 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc
     3721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     3781 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
     3841 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3901 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     3961 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4021 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     4081 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     4141 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     4201 acgacaaggt gaggaactaa accatggcca agcctttgtc tcaagaagaa tccaccctca
     4261 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     4321 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     4381 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     4441 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     4501 gtcgacaggt gcttctcgat ctgcatcctg ggatcaaagc gatagtgaag gacagtgatg
     4561 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     4621 aagcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     4681 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     4741 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     4801 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4861 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     4921 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     4981 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5041 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5101 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5161 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5221 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5281 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5341 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5401 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5461 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5521 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     5581 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5641 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5701 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5761 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     5821 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5881 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5941 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6001 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6061 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6121 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6181 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6241 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6301 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6361 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6421 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6481 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6541 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6601 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6661 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6721 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6781 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6841 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6901 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6961 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7021 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7081 tttccccgaa aagtgccacc tgacgtc
//
