LOCUS       Exported                4135 bp DNA     circular SYN 30-NOV-2021
DEFINITION  DA-pBM10a pSC101] T7 degron TALEs ACA pro1 sfGFP bla.
ACCESSION   urn.local...bn-cn5uk4x
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
COMMENT     Imported From
            Path: 
            /Users/bmok/Library/Containers/com.apple.mail/Data/Library/Mail 
            Downloads/342C1E5A-338E-4E52-BE62-496B07588D7F
            Filename: pTPH424 - base editing library.gb
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="synthetic DNA construct"
                     /lab_host="."
                     /mol_type="other DNA"
     source          94..106
                     /note="/dnas_title=pTW002a - CP ColEI Rop dnak-P1 SD8 LuxAB
                     tetA-orfL aadA"
                     /note="color: #ffffff"
     source          94..106
                     /note="/dnas_title=pTR4 - AP SC101 PhaPO g3-luxAB TetR SD8 
                     PhaR-2 Term"
                     /note="color: #ffffff"
     source          107..1178
                     /note="/dnas_title=pTW002a - CP ColEI Rop dnak-P1 SD8 LuxAB
                     tetA-orfL aadA"
                     /note="color: #ffffff"
     source          107..1178
                     /note="/dnas_title=pTW001b - AP SC101 RepA Ptet SD8 MBP(wt)
                     bla"
                     /note="color: #ffffff"
     source          join(1179..2938,1..93)
                     /note="/NCBI Join Type=join"
                     /note="/dnas_title=pTW001a - AP SC101 Ptet SD8 gIV bla"
                     /note="This feature has 2 segments:
                        1: 1179 .. 2938 / #ffffff
                        2:    1 ..   93 / #ffffff"
     source          2939..4135
                     /note="/dnas_title=CE-SP-KanR"
                     /note="color: #ffffff"
     source          2939..2951
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          2952..3141
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          3045..3083
                     /note="/dnas_title=CE-SP-KanR"
                     /note="color: #ffffff"
     source          3045..3083
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          3230..3244
                     /note="/dnas_title=pTW002a - CP ColEI Rop dnak-P1 SD8 LuxAB
                     tetA-orfL aadA"
                     /note="color: #ffffff"
     source          3230..3244
                     /note="/dnas_title=pTR4 - AP SC101 PhaPO g3-luxAB TetR SD8 
                     PhaR-2 Term"
                     /note="color: #ffffff"
     source          3245..3967
                     /note="/dnas_title=pTW002a - CP ColEI Rop dnak-P1 SD8 LuxAB
                     tetA-orfL aadA"
                     /note="color: #ffffff"
     source          3245..3967
                     /note="/dnas_title=pAB055k - AP pSC101 rrnB1(full) pTet SD8
                     luxAB bla"
                     /note="color: #ffffff"
     source          3245..3967
                     /note="/dnas_title=QuadTerm Reverse pJC173b - AP pSC101 pT7
                     SD8 RBS g3dp-luxAB ggggs fusion"
                     /note="color: #ffffff"
     source          3968..3982
                     /note="/dnas_title=pTW002a - CP ColEI Rop dnak-P1 SD8 LuxAB
                     tetA-orfL aadA"
                     /note="color: #ffffff"
     source          3968..3982
                     /note="/dnas_title=pTR4 - AP SC101 PhaPO g3-luxAB TetR SD8 
                     PhaR-2 Term"
                     /note="color: #ffffff"
     source          3968..3982
                     /note="/dnas_title=pBAD HIS-TEV-T7RNAP aadA colE1"
                     /note="color: #ffffff"
     source          3983..4135
                     /note="/dnas_title=pAB078d3 - AP RepA SC101 
                     pLac61*(434-OR1-m13_16) SD8 luxAB pro1 SD8 
                     434-cI-Flag-ABL1-SH2 rrnB1(full) Term bla"
                     /note="color: #ffffff"
     source          4039..4079
                     /note="/dnas_title=pAB078d3 - AP RepA SC101 
                     pLac61*(434-OR1-m13_16) SD8 luxAB pro1 SD8 
                     434-cI-Flag-ABL1-SH2 rrnB1(full) Term bla"
                     /note="color: #ffffff"
     primer_bind     1..20
                     /label=TPH1414
                     /note="color: black; sequence: ACTTAATTAACGGCACTGCG"
     misc_feature    1..17
                     /label=USER linker
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      complement(18..51)
                     /label=BBa_B1002 terminator
                     /note="color: #ffffff; direction: LEFT"
     terminator      complement(52..93)
                     /label=tetA/orfL bidirectional terminator
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(85..106)
                     /label=389
                     /note="color: black; sequence: ACGCCATGGGCATTAAAAAATG"
     primer_bind     94..129
                     /label=128
                     /note="color: black; sequence: 
                     ATGCCCATGGCGTTTACCAATGCTTAATCAGTGAGG"
     modified_base   94..106
                     /label=USER junction modified base
                     /note="USER junction"
                     /note="/modified_by=tinawang"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             complement(107..967)
                     /label=bla (AmpR) CDS
                     /note="bla (AmpR)"
                     /note="from pUC19"
                     /note="color: #993366; direction: LEFT"
     promoter        complement(968..1162)
                     /label=bla promoter
                     /note="bla promoter"
                     /note="from pUC19"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(1144..1178)
                     /label=JC264
                     /note="color: black; sequence: 
                     ACGGCCTATTTGGCCTAAGGGCCTCGTGATACGCC"
     modified_base   1163..1178
                     /label=USER junction modified base
                     /note="USER junction"
                     /note="/modified_by=tinawang"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             complement(1179..2129)
                     /label=RepA CDS
                     /note="RepA"
                     /note="pSC101 origin of replication protein"
                     /note="color: #993366; direction: LEFT"
     rep_origin      complement(2133..2878)
                     /direction=LEFT
                     /label=pSC101 rep origin
                     /note="pSC101"
                     /note="color: #ffff00"
     primer_bind     2673..2691
                     /label=1274
                     /note="color: black; sequence: TACAAGAAAAGCCCGTCAC"
     primer_bind     2769..2792
                     /label=AB0399
                     /note="color: black; sequence: CTGATTTTCCAGTCTGACCACTTC"
     modified_base   2879..2893
                     /label=USER junction modified base
                     /note="USER junction"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      2894..2938
                     /label=rrnB1 transcriptional terminator
                     /note="rrnB1 transcriptional terminator"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     2898..2916
                     /label=TPH1415
                     /note="color: black; sequence: 
                     ACGTGCTCAGTCTAAAACGAAAGGCCCAGTC"
     primer_bind     complement(2921..2951)
                     /label=TPH1415
                     /note="color: black; sequence: 
                     ACGTGCTCAGTCTAAAACGAAAGGCCCAGTC"
     primer_bind     2933..2957
                     /label=BM1811-HTS
                     /note="color: black; sequence: CGTTTTAGACTGAGCACGTCAATAC"
     primer_bind     2939..2971
                     /label=1443
                     /note="color: black; sequence: 
                     AGACTGAGCACGTCAATACGCAAACCGCCTCTC"
     modified_base   2939..2951
                     /label=USER 55
                     /note="/created_by=tinawang"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(3005..3023)
                     /label=BM1796
                     /note="color: black; sequence: AGTCGGGAAACCTGTCGTG"
     primer_bind     complement(3008..3057)
                     /label=BM1817
                     /note="color: black; sequence: 
                     AACGCGAAGTCGGACTACGAATAGGTATACTCCAGTCGGGAAACCTGTC"
     misc_feature    complement(3008..3027)
                     /label=annealing
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(3008..3025)
                     /label=TPH1937
                     /note="color: black; sequence: CCAGTCGGGAAACCTGTC"
     primer_bind     3011..3028
                     /label=BM1794
                     /note="color: black; sequence: 
                     AGGTTTCCCGACTGGAGTGAGCGCAACGCC"
     misc_feature    3026..3084
                     /label=BM1798 (idt gene block)
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3028..3042
                     /label=UMI
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3045..3057
                     
                     /note="Geneious type: DNA probe bind"
                     /note="/%GC=61.5"
                     /note="/Hairpin Tm=38.2"
                     /note="/Self Dimer Tm=5.8"
                     /note="/Sequence=CCGACTTCGCGTT"
                     /note="/Tm=48.5"
                     /note="/created by=bmok"
                     /note="/created by=primer3"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     3048..3088
                     /label=BM1816
                     /note="color: black; sequence: 
                     ACTTCGCGTTTTTTTTGTTTATTTCAGCAAACGACGAACCC"
     misc_feature    3063..3065
                     /label=ACA
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3066..3088
                     /label=anneal
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(3073..3083)
                     /label=T7 RNAP binding site 4
                     /note="Geneious type: DNA probe bind"
                     /note="/%GC=54.5"
                     /note="/Hairpin Tm=None"
                     /note="/Self Dimer Tm=None"
                     /note="/Sequence=CGTCGTTTGCT"
                     /note="/Tm=38.0"
                     /note="/created by=primer3"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     3092..3107
                     /label=BM1797
                     /note="color: black; sequence: AAACGCGCCCTGACGG"
     misc_feature    3092..3093
                     /label=modified
                     /note="Geneious type: modified base "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3109
                     
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     primer_bind     complement(3116..3129)
                     /label=BM1812-HTS
                     /note="color: black; sequence: CGGATGCCGGGAGC"
     primer_bind     complement(3123..3154)
                     /label=1444
                     /note="color: black; sequence: 
                     ACATTGGCTCCGTCTTGTCTGTAAGCGGATGC"
     primer_bind     complement(3123..3141)
                     /label=1364 annealing region
                     /note="color: black; sequence: CTTGTCTGTAAGCGGATGC"
     primer_bind     3142..3165
                     /label=299
                     /note="color: black; sequence: ACGGAGCCAATGTACGCAAAAAAC"
     modified_base   3142..3154
                     /label=USER
                     /note="/created_by=tinawang"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      3156..3189
                     /label=BBa B1002 terminator
                     /note="color: #ffffff; direction: RIGHT"
     terminator      complement(3191..3229)
                     /label=BBa B1006 terminator
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(3211..3244)
                     /label=28
                     /note="color: black; sequence: 
                     AGTCAGTAGGGCCCTAAAAAAAAACCCCGCCCCT"
     primer_bind     3230..3268
                     /label=1398
                     /note="color: black; sequence: 
                     AGGGCCCTACTGACTTTATGAGCCTTTATACAGTTCGTC"
     modified_base   3230..3244
                     /label=USER junction
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             complement(3245..3967)
                     /label=sfGFP
                     /note="/created_by=User"
                     /note="color: #993366; direction: LEFT"
     primer_bind     complement(3745..3766)
                     /label=TW0297
                     /note="color: black; sequence: GTTCAGTGTTTCAGTCGTTACC"
     primer_bind     complement(3949..4006)
                     /label=1399
                     /note="color: black; sequence: 
                     ATTTTGTTTAACTTTTACTAGAGTAAGGAGGAAAAAAAAATGAGCAAGGGCGAAGAAC"
     RBS             complement(3968..3982)
                     /label=SD8 RBS
                     /note="SD8"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     3983..4028
                     /label=TW0126
                     /note="color: black; sequence: 
                     ACTCTAGTAAAAGTTAAACAAAATTATTTGTAGAGGGAAACCGTTG"
     modified_base   3983..4006
                     /label=USER Linker modified base
                     /note="USER Linker"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(3992..4038)
                     /label=insulation
                     /note="/created_by=tinawang"
                     /note="color: #a6acb3; direction: LEFT"
     prim_transcript 4038
                     /label=+1 prim transcript
                     /note="+1"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        complement(4039..4079)
                     /label=pro1
                     /note="pro1 (Insulated Promoter - based on rrnB1 P1)"
                     /note="/modified_by=User"
                     /note="color: #ffffff; direction: LEFT"
     -10_signal      complement(4045..4050)
                     /label=-10
                     /note="-10"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: LEFT"
     -35_signal      complement(4068..4073)
                     /label=-35
                     /note="-35"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(4074..4135)
                     /label=insulation
                     /note="/created_by=tinawang"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(join(4117..4135,1..17))
                     /label=TPH1413
                     /note="color: black; sequence: 
                     AGTGCCGTTAATTAAGTCACAGCTAACACCACGTCG"
ORIGIN
        1 acttaattaa cggcactgcg aaaaaacccc gccgaagcgg ggttttttgc gaaatataat
       61 gaccctcttg ataacccaag agggcatttt ttaatgccca tggcgtttac caatgcttaa
      121 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      181 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
      241 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
      301 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
      361 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
      421 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
      481 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
      541 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
      601 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
      661 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
      721 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
      781 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
      841 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      901 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      961 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1021 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1081 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     1141 ataggcgtat cacgaggccc ttaggccaaa taggccgttc agatccttcc gtatttagcc
     1201 agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga
     1261 tcatgcttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt
     1321 gcagttaaag catcgtgtag tgtttttctt agtccgttac gtaggtagga atctgatgta
     1381 atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg
     1441 agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc
     1501 ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa
     1561 acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta
     1621 aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt
     1681 aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga
     1741 ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact
     1801 aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat ctcaaagcct
     1861 ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct
     1921 aatacaccat aagcattttc cctactgatg ttcatcatct gagcgtattg gttataagtg
     1981 aacgataccg tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca
     2041 cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca
     2101 tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca
     2161 ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct ttgagttgtg
     2221 ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct
     2281 gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat
     2341 agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca
     2401 ctactttagt cagttccgca gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta
     2461 caaaacagac cttaaaaccc taaaggctta agtagcaccc tcgcaagctc gggcaaatcg
     2521 ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat
     2581 tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct
     2641 tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc
     2701 agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt
     2761 cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac
     2821 tggctaatgc acccagtaag gcagcggtat catcaacagg cttacccgtc ttactgtcaa
     2881 gaggacatcc ggtcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttag
     2941 actgagcacg tcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3001 ctggcacgac aggtttcccg actggagttt gtagtctagt ctgtccgact tcgcgttttt
     3061 tttgtttatt tcagcaaacg acgaacccgc caaacgcgcc ctgacgggat tgtctgctcc
     3121 cggcatccgc ttacagacaa gacggagcca atgtacgcaa aaaaccccgc ttcagcgggg
     3181 ttttttcgcc aaaaaaaacc ccgccctgtc aggggcgggg ttttttttta gggccctact
     3241 gactttatga gcctttatac agttcgtcca taccgtgcgt gatgccggcc gcggtcacga
     3301 attccagcag gaccatgtgg tcacgctttt cgttcggatc tttactcagc acagactgcg
     3361 tgctcaggta atgattgtcc ggcagcagga ccgggccatc accaatcggg gtgttttgct
     3421 gatagtggtc ggccagttga acgctgccat cttccacatt atggcggatc ttgaagttag
     3481 ccttgatacc gttcttctgc ttatctgcgg tgatgtacac gttgtgcgaa ttgaagttat
     3541 attccagttt atggcccaga atattaccgt cttccttaaa atcgatgcct ttcagttcaa
     3601 tacggttaac cagcgtatca ccttcgaatt tcacttcggc gcgcgttttg taggtgccgt
     3661 catctttaaa tgagatggta cgttcttgga cataaccttc cggcatcgcg gacttgaaaa
     3721 agtcgtggcg tttcatgtga tccgggtaac gactgaaaca ctgaacacca tacgtcagcg
     3781 tggtgaccag ggtcggccac ggaaccggca gtttacccgt ggtgcaaata aacttcagcg
     3841 tcagtttacc attggttgca tcgccttcac cttcgccgcg cacgctgaat ttgtgaccat
     3901 tgacatcacc atccagttcc accagaatcg gcaccacgcc cgtaaacagt tcttcgccct
     3961 tgctcatttt tttttcctcc ttactctagt aaaagttaaa caaaattatt tgtagaggga
     4021 aaccgttgtg gtctccctga atatagatac cgagccttat gcatgcccgt aaagttatcc
     4081 agcaaccact catagaccta gggcagcaga tagggacgac gtggtgttag ctgtg
//
