LOCUS       Exported                6093 bp DNA     circular SYN 30-NOV-2021
DEFINITION  [ColE1] pBAD sd4U UGITALE3 evolved G1397 N (wildtype)SmR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Thuronyi B Liu DR
  TITLE     Continuous evolution of base editors with expanded target 
            compatibility and improved activity
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2019 from SnapGene Server 1.1.58 
            http://www.snapgene.com
REFERENCE   3  (bases 1 to 6093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Exported 
            Mar 19, 2019 from SnapGene Server 1.1.58 http://www.snapgene.com"
COMMENT     Imported From
            Path: /Users/bmok/Downloads
            Filename: addgene-plasmid-122595-sequence-238421.gbk
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="synthetic DNA construct"
                     /mol_type="genomic DNA"
     primer_bind     15..39
                     /label=BM1800
                     /note="color: black; sequence: 
                     ACTTAGGGACTCTCAAATAAAACGAAAGGCTCAGTCG"
     primer_bind     complement(62..82)
                     /label=BM1802
                     /note="color: black; sequence: AGAGTCCCTAAGTCTCCTCAG"
     primer_bind     70..106
                     /label=BM1800
                     /note="color: black; sequence: 
                     ACTTAGGGACTCTCAAATAAAACGAAAGGCTCAGTCG"
     misc_feature    83..1369
                     /label=from MP6
                     /note="Geneious type: insertion "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      83..128
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB  
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             complement(143..1021)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /note="color: #993366"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1048..1332
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the 
                     oppositedirection (Guzman et al., 1995)"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     1256..1275
                     /label=pBAD Forward
                     /note="color: black; sequence: TGCCATAGCATTTTTATCCA"
     -10_signal      1319..1324
                     /label=-10
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(1348..1394)
                     /label=BM1866
                     /note="color: black; sequence: 
                     AGGTTGGTCAATTTTTCCTTTTTTTTGTCAGACTTGAGTTGTACGCG"
     primer_bind     complement(1349..1403)
                     /label=BM1801
                     /note="color: black; sequence: 
                     ATGTCAGACAGGTTGGTCATTTTTTTTTCCTCCTTATTTTTGTCAGACTTGAGTTGTAC
                     GC"
     RBS             1370..1384
                     /label=sd4U
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     1383..1404
                     /label=BM1865
                     /note="color: black; sequence: ATTGACCAACCTGTCTGACATC"
     misc_feature    1384..1386
                     /label=start
                     /note="Geneious type: start"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_difference 1387..1647
                     /label=from TC 30
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             1387..1635
                     /label=UGI (from PACE2 T6 D03)
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     1390..1410
                     /label=BM1501
                     /note="color: black; sequence: AACCTGTCTGACATCATCGAG"
     primer_bind     complement(1422..1441)
                     
                     /note="color: black; sequence: CCTGGATGACCAGCTGCTTG"
     misc_feature    1525..1527
                     /label=Asp > Asn
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     1564..1582
                     /label=BM477 (mismatched first base)
                     /note="color: black; sequence: AACGCCCCAGAGTATAAGC"
     misc_feature    1564..1566
                     /label=Asp>Asn
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(1620..1641)
                     /label=BM1413
                     /note="color: black; sequence: ACCAGACAGCATTTTGATTTTG"
     primer_bind     1630..1648
                     /label=BM1790
                     /note="color: black; sequence: ATGCTGTCTGGTGGTTCTG"
     CDS             1648..2061
                     /label=NTD (PACE 2 T6-D03)
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     1696..1715
                     /label=BM1470
                     /note="color: black; sequence: AAGATCAAGCCTAAGGTGCG"
     primer_bind     complement(1719..1734)
                     /label=BM1725
                     /note="color: black; sequence: GTGCTGGGCGACTGTG"
     primer_bind     2045..2061
                     /label=BM1471
                     /note="color: black; sequence: CCGGAGCCCCTCTAAAC"
     CDS             2062..3345
                     /label=T7 RNAP TALE binding site 3 - PACE 2 T6-D03
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    2161..2163
                     /label=Gly > Glu
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2173..2175
                     /label=Glu > Lys
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2221..2223
                     /label=Glu > Lys
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_difference 2875..2877
                     
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2898
                     /label=A>G
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     misc_feature    3139..3141
                     /label=Glu > Lys
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             3346..3468
                     /label=CTD from PACE2-T6-D03
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(3347..3363)
                     /label=BM1472
                     /note="color: black; sequence: CAGCTGGGCCACAATGC"
     misc_feature    3445..3447
                     /label=Asp > Asn
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(3448..3464)
                     /label=BM1502
                     /note="color: black; sequence: AGGCCCTTTTTCACGGC"
     primer_bind     complement(3467..3481)
                     /label=BM1733-correct
                     /note="color: black; sequence: AGCTGCCGGATCCAC"
     primer_bind     3471..3485
                     /label=BM1732
                     /note="color: black; sequence: ATCCGGCAGCTACGC"
     CDS             3475..3798
                     /label= DddE(41-148) wildtype
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3502..3543
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(3514..3533)
                     /label=BM476
                     /note="color: black; sequence: CCATTGTAAGCTGGCAGCTG"
     primer_bind     complement(3528..3546)
                     /label=BM1852
                     /note="color: black; sequence: ACCCACGGTCTGACCATTG"
     primer_bind     3538..3568
                     /label=BM1850
                     /note="color: black; sequence: 
                     ACCGTGGGTGCCTTCTACTATGTGAACGACG"
     primer_bind     3548..3633
                     /label=BM1849
                     /note="color: black; sequence: 
                     GCCTTCTACTATGTGAACGACGCGGGCGGTCTGGAGAGCAAGGTGTTTAGCAGCGGCGG
                     TCCAACCCCGTACCCAAACTATGCCAAT"
     misc_feature    3562..3573
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3595..3606
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(3613..3636)
                     /label=BM1851
                     /note="color: black; sequence: ACGGGCATTGGCATAGTTTGGGTACGG"
     primer_bind     3631..3648
                     /label=BM1853
                     /note="color: black; sequence: AATGCCCGTCATGTGGAG"
     regulatory      3640..3642
                     /label=His96-chelate to zinc
                     /note="/created by=bmok"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    3646..3648
                     
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3679..3687
                     /label=loop (SPLIT)
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     regulatory      3724..3726
                     
                     /note="/created by=bmok"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    3754..3765
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3781..3798
                     
                     /note="Geneious type: polylinker"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(3784..3811)
                     /label=BM1804 (tm 65)
                     /note="color: black; sequence: 
                     ATTAAGTTTATTAACCTTCCGGCGGCAC"
     misc_feature    3796..3798
                     
                     /note="Geneious type: Sequence difference"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3799..3804
                     /label=stop
                     /note="Geneious type: stop"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     3800..3825
                     /label=BM1805
                     /note="color: black; sequence: AATAAACTTAATTAACGGCACTCCTC"
     terminator      complement(3845..3886)
                     /note="/created by=bmok"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(3896..3915)
                     /label=BM1578-HTS
                     /note="color: black; sequence: GGTAGTCGGCAAATAAACGC"
     CDS             complement(3900..4691)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,  
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and  
                     streptomycin"
                     /note="color: #993366"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     complement(3978..3998)
                     /label=BM1465
                     /note="color: black; sequence: CTTGGACAAGAAGAAGATCGC"
     promoter        complement(4692..4763)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      4791..5379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
                     /note="color: #ffff00"
     primer_bind     5281..5300
                     /label=pBR322ori-F
                     /note="color: black; sequence: GGAAACGCCTGGTATCTTTA"
     primer_bind     5534..5551
                     /label=L4440
                     /note="color: black; sequence: GCGAGTCAGTGAGCGAGG"
     misc_feature    5565..5705
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(5632..5651)
                     /label=pRS-marker
                     /note="color: black; sequence: GCGGCATCAGAGCAGATTGT"
     primer_bind     5792..5814
                     /label=pGEX 3'
                     /note="color: black; sequence: CGGGAGCTGCATGTGTCAGAGGT"
     CDS             complement(5807..5998)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /note="color: #993366"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     6063..6082
                     /label=BM1460
                     /note="color: black; sequence: ATGCTCACGATACGGGTTAC"
ORIGIN
        1 aagaggacat ccggtcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
       61 gctgaggaga cttagggact ctcaaataaa acgaaaggct cagtcgaaag actgggcctt
      121 tcgttttaga cttagggacc ctttatgaca acttgacggc tacatcattc actttttctt
      181 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
      241 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
      301 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
      361 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
      421 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
      481 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
      541 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
      601 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
      661 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
      721 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
      781 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
      841 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
      901 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
      961 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
     1021 tacttttcat actcccacca ttcagagaag aaaccaattg tccatattgc atcagacatt
     1081 gccgtcactg cgtcttttac tggctcttct cgctaaccca accggtaacc ccgcttatta
     1141 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
     1201 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
     1261 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
     1321 ctgtttctcc atacccgttt ttttggacgc gtacaactca agtctgacaa aaaaaaggaa
     1381 aaattgacca acctgtctga catcatcgag aaggagacag gcaagcagct ggtcatccag
     1441 gagagcatcc tgatgctgcc cgaagaagtc gaagaagtga tcggaaacaa gcctgagagc
     1501 gatatcctgg tccataccgc ctacaacgag agtaccgatg aaaatgtgat gctgctgaca
     1561 tccaacgccc cagagtataa gccctgggct ctggtcatcc aggattccaa cggagagaac
     1621 aaaatcaaaa tgctgtctgg tggttctgac atcgctgatc tgagaaccct gggctactcc
     1681 cagcagcaac aggagaagat caagcctaag gtgcggagca cagtcgccca gcaccacgag
     1741 gccctggtgg gccacggctt cacccacgcc cacatcgtgg ccctcagcca gcatcctgcc
     1801 gccctgggga ccgtggccgt gaagtaccag gacatgatcg cggctctgcc agaagccacc
     1861 cacgaagcca tcgtgggcgt gggcaagcaa tggtccggcg ccagagccct ggaggccctg
     1921 ctgaccgtgg ccggtgagct gagaggccct cctctgcagc tggacaccgg ccagctgctc
     1981 aagatcgcta agcggggcgg agtaaccgct gtcgaggccg tgcacgcctg gcggaacgcc
     2041 ctaaccggag cccctctaaa cctgacccca gagcaggtcg tggcaatcgc aagccacgat
     2101 ggcggcaagc aggccctgga aacagtgcag gccctgctac ctgtcctgtg ccaggcccac
     2161 gaactgaccc ccaagcaggt ggtagccatc gccagccacg atggcggaaa acaggccctg
     2221 aaaacagtgc agcggctgct gcccgtgctg tgccaggccc acggcctgac ccctcaacag
     2281 gtcgtggcca tcgctagcaa caacggcggc aagcaggccc tggagactgt gcagagattg
     2341 ctgcctgtgc tgtgccaggc ccacggcctg acccctgagc aggtggtggc tatcgcctct
     2401 aacatcggcg gcaagcaggc cctggagacc gttcaggcac tgctacctgt tctgtgtcag
     2461 gctcacggac tgacacctga gcaggtggtg gcaatcgcca gccacgacgg cggcaagcag
     2521 gccttggaaa cagtgcaggc cctgctgcca gtgctgtgcc aggcccacgg cctgacccct
     2581 gagcaggtgg tagccattgc cagcaacggc ggaggcaaac aagccctgga aaccgtgcag
     2641 gctctgctgc ctgtgctgtg tcaagcccat ggcctcacac ccgagcaggt ggtggctatc
     2701 gcctccaatg gaggcggcaa acaggccctc gagaccgtgc agagactgct gcctgtgctg
     2761 tgccaggccc acggcctgac acccgaacaa gttgtggcaa tcgcctctca cgacggcgga
     2821 aaacaggccc tggaaaccgt gcaggctctt ctgcccgtgc tgtgtcaggc ccacgaactg
     2881 acccccgagc aggtggtggc tatcgcaagc aacaacggcg gcaagcaggc cctggaaaca
     2941 gtccaaaggc tgctgcccgt gctgtgccag gcccacggcc tgacacctga gcaggtggtg
     3001 gccatcgcca gccatgacgg cggcaagcag gcacttgaaa ccgttcaggc tctgctgccc
     3061 gtcctgtgcc aggcccacgg cctaacccct gaacaagtgg tggccatcgc ctcaaacaat
     3121 ggcggcaagc aagccctgaa aaccgtgcag gccctgctgc ctgtcctgtg ccaggcccac
     3181 ggactgaccc ccgagcaggt ggtagccatc gcatctaatg gcggagggaa gcaggctctg
     3241 gagacggtgc aagcactcct gcccgtgctg tgccaggccc acggcctgac cccccagcaa
     3301 gtggttgcta tcgccagcaa cggcggcggt agaccagccc tggagagcat tgtggcccag
     3361 ctgagcagac ctgatcctgc cctggccgcc ctgaccaacg accacctggt ggccctggcc
     3421 tgcctgggcg gaagacccgc tttgaacgcc gtgaaaaagg gcctgggtgg atccggcagc
     3481 tacgccctgg gtccgtatca gattagcgcc ccgcagctgc cagcttacaa tggtcagacc
     3541 gtgggtacct tctactatgt gaacgacgcg ggcggtctgg agagcaaggt gtttagcagc
     3601 ggcggtccaa ccccgtaccc aaactatgcc aatgccggtc atgtggaggg tcagagcgcc
     3661 ctgttcatgc gtgataacgg catcagcgag ggtctggtgt tccacaacaa cccggaaggc
     3721 acctgcggtt tttgcgtgaa catgaccgag accctgctgc cggaaaacgc gaaaatgacc
     3781 gtggtgccgc cggaaggtta ataaacttaa ttaacggcac tcctcagcaa aggcactcct
     3841 cagcaaatat aatgaccctc ttgataaccc aagagggcat tttttaatgc ccatggcgtt
     3901 tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga
     3961 tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt
     4021 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc
     4081 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt
     4141 cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc
     4201 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag
     4261 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct
     4321 ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc
     4381 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg
     4441 cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct
     4501 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt
     4561 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg
     4621 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc
     4681 gcttccctca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4741 atgagcgtat acatatttga atgtatttag aaaaataggc caaatagggt ttgagatcct
     4801 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4861 tgtttgccgg atcaagagct accacctctt tttccgaagg taactggctt cagcagagcg
     4921 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     4981 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5041 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5101 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5161 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5221 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5281 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5341 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5401 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5461 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5521 acgaccgagc gcagcgagtc agtgagcgag gaagcggatg agcgcctgat gcggtatttt
     5581 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     5641 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     5701 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5761 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5821 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg
     5881 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
     5941 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
     6001 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga
     6061 ggatgctcac gatacgggtt actgatgatg aac
//
