LOCUS       Exported                6555 bp DNA     circular UNA 30-NOV-2021
DEFINITION  [p15a]  pBAD sd2 UGI- T4 evolved TALE-Left-DddA C T1413I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
COMMENT     Imported From
            Path: 
            /Users/bmok/Library/Containers/com.apple.mail/Data/Library/Mail 
            Downloads/841DB3B4-3A54-42AD-B232-04FCE134AAF2
            Filename: 
            mn147-from-mn88-pbad33-with-tale2dddac-and-spr-but-with-cbpartsremov
            ed.gb
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="synthetic DNA construct"
                     /mol_type="genomic DNA"
     misc_feature    complement(1..884)
                     /label=from pBM15
                     /note="Geneious type: motif"
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(1..816)
                     /label=kan2 marker
                     /note="Geneious type: Marker"
                     /note="/Imported from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Plas
                     Mapper</a>"
                     /note="/Transferred From=kan2 marker"
                     /note="/Transferred Similarity=99.75%"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     3..27
                     /label=BM1939
                     /note="color: black; sequence: AGAAAAACTCATCGAGCATCAAATG"
     primer_bind     complement(12..34)
                     /label=BM1473
                     /note="color: black; sequence: GCAGTTTCATTTGATGCTCGATG"
     primer_bind     725..744
                     /label=Kan-R
                     /note="color: black; sequence: TCGCGAGCCCATTTATACCC"
     primer_bind     809..828
                     /label=pENTR-R
                     /note="color: black; sequence: TGGCTCATAACACCCCTTGT"
     primer_bind     complement(860..894)
                     /label=BM1940
                     /note="color: black; sequence: 
                     AATGAGACGTGATCCGTAGGTTTCTCTTCATGAAC"
     primer_bind     885..905
                     /label=BM1941
                     /note="color: black; sequence: ACGTCTCATTTTCGCCAAAAG"
     misc_feature    1143..2059
                     /label=p15A origin
                     /note="Geneious type: Origin of Replication"
                     /note="/Imported from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Plas
                     Mapper</a>"
                     /note="/Transferred From=p15A origin"
                     /note="/Transferred Similarity=94.41%"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(1277..1294)
                     /label=L4440
                     /note="color: black; sequence: TAGCGAGTCAGTGAGCGA"
     misc_feature    1295..1312
                     /label=MN364
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /note="color: #a6acb3; direction: RIGHT"
     rep_origin      complement(1410..1955)
                     /direction=LEFT
                     /label=p15A ori
                     /note="/ApEinfo_fwdcolor=#f58a5e"
                     /note="/ApEinfo_revcolor=#f58a5e"
                     /note="color: #ffff00"
     primer_bind     complement(1491..1510)
                     /label=pBR322ori-F
                     /note="color: black; sequence: GGGGAAACGCCTGGTATCTT"
     primer_bind     1772..1790
                     /label=BM1973
                     /note="color: black; sequence: AGTCTTGAAGTCATGCGCC"
     primer_bind     complement(1848..1869)
                     /label=BM1974
                     /note="color: black; sequence: CTACCAACTCTTTGAACCGAGG"
     primer_bind     1943..1976
                     /label=MN365
                     /note="color: black; sequence: 
                     AAAACGATCTCAAGAAGATCATCTTATTAATCAG"
     primer_bind     2032..2051
                     /label=pBRrevBam
                     /note="color: black; sequence: GTGATGTCGGCGATATAGGC"
     primer_bind     complement(2206..2242)
                     /label=BM2050
                     /note="color: black; sequence: 
                     AGAGTCCCTAAGTTTGGTAACGAATCAGACAATTGAC"
     misc_feature    2230..5424
                     /label=from DA-pBM14-sd2
                     /note="Geneious type: insert"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     2234..2257
                     /label=BM2049
                     /note="color: black; sequence: AGGGACTCTCAAATAAAACGAAAG"
     terminator      2243..2288
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB  
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             complement(2303..3181)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /note="color: #993366"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     2630..2650
                     /label=MN366
                     /note="color: black; sequence: CTGACAAGCCTCGCGTACCCG"
     primer_bind     3139..3155
                     /label=BM1807
                     /note="color: black; sequence: ACGAGTATCCCGGCAGC"
     misc_feature    3208..3529
                     /label=from MP6
                     /note="Geneious type: insertion "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        3208..3492
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the 
                     oppositedirection (Guzman et al., 1995)"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3416..3435
                     /label=pBAD Forward
                     /note="color: black; sequence: TGCCATAGCATTTTTATCCA"
     -10_signal      3479..3484
                     /label=-10
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(3508..3552)
                     /label=BM1868
                     /note="color: black; sequence: 
                     ATTAGTCAATTTTTCCTTTTTTTTGTCAGACTTGAGTTGTACGCG"
     primer_bind     complement(3509..3563)
                     /label=BM1813
                     /note="color: black; sequence: 
                     ATGTCGCTCAGATTAGTCATTTTTTTTTCCTCCTTATTTTTGTCAGACTTGAGTTGTAC
                     GC"
     RBS             3530..3544
                     /label=sd4U
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     3541..3568
                     /label=BM1867
                     /note="color: black; sequence: 
                     AAATTGACTAATCTGAGCGACATCATTG"
     misc_feature    3544..3546
                     /label=start
                     /note="Geneious type: start"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             3547..3795
                     /label=UGI from PACE2-T6-C03
                     /note="/created by=kzhao"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     3547..3570
                     /label=BM1624
                     /note="color: black; sequence: ACTAATCTGAGCGACATCATTGAG"
     misc_feature    3622..3624
                     /label=Glu > Lys
                     /note="Geneious type: modified base "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3631..3633
                     /label=Gly > Lys
                     /note="Geneious type: modified base "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     3767..3789
                     /label=BM716
                     /note="color: black; sequence: CTAACGGCGAGAATAAGATCAAG"
     primer_bind     complement(3773..3819)
                     /label=BM1625
                     /note="color: black; sequence: 
                     ATCGGCGATGTCAGAACCACCAGACAGCATCTTGATCTTATTCTCGC"
     CDS             3808..4221
                     /label=wild type NTD (taken from mouse mito 95)
                     /note="/created by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3808..3810
                     /label=Asp > Asn
                     /note="Geneious type: modified base "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     3809..3824
                     /label=BM1626
                     /note="color: black; sequence: ACATCGCCGATCTGCG"
     primer_bind     complement(3820..3833)
                     /label=BM1651
                     /note="color: black; sequence: CCCAGGGTCCGCAG"
     primer_bind     complement(3878..3892)
                     /label=BM1590
                     /note="color: black; sequence: CTCAGCTGAGCCACGATAG"
     misc_feature    4105..4107
                     /label=Gly > Glu
                     /note="Geneious type: modified base "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(4201..4217)
                     /label=BM1579
                     /note="color: black; sequence: AGAGGAGCTCCGGTCAG"
     primer_bind     4203..4221
                     /label=BM1589
                     /note="color: black; sequence: GACCGGAGCTCCTCTGAAC"
     primer_bind     4204..4251
                     /label=BM1627
                     /note="color: black; sequence: 
                     ACCGGAGCTCCTCTGAACCTGACCCCAGAACAGGTTGTGGCTATCGCC"
     CDS             4222..5301
                     /label=T7 RNAP 4 from PACE2-T6-C03
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     modified_base   4227
                     /label=A>C
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #a6acb3"
     modified_base   4230
                     /label=T>A
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     modified_base   4233
                     /label=G>A
                     /note="/created by=bmok"
                     /note="color: #a6acb3"
     primer_bind     4438..4455
                     /label=BM1627
                     /note="color: black; sequence: 
                     ACCGGAGCTCCTCTGAACCTGACCCCAGAACAGGTTGTGGCTATCGCC"
     primer_bind     4527..4557
                     /label=BM1627
                     /note="color: black; sequence: 
                     ACCGGAGCTCCTCTGAACCTGACCCCAGAACAGGTTGTGGCTATCGCC"
     primer_bind     4731..4761
                     /label=BM1627
                     /note="color: black; sequence: 
                     ACCGGAGCTCCTCTGAACCTGACCCCAGAACAGGTTGTGGCTATCGCC"
     primer_bind     4935..4965
                     /label=BM1627
                     /note="color: black; sequence: 
                     ACCGGAGCTCCTCTGAACCTGACCCCAGAACAGGTTGTGGCTATCGCC"
     primer_bind     5139..5169
                     /label=BM1627
                     /note="color: black; sequence: 
                     ACCGGAGCTCCTCTGAACCTGACCCCAGAACAGGTTGTGGCTATCGCC"
     CDS             5302..5424
                     /label=C-terminal domain from PACE2-T6-C03
                     /note="/created_by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(5303..5321)
                     /label=BM1590
                     /note="color: black; sequence: CTCAGCTGAGCCACGATAG"
     primer_bind     5305..5321
                     /label=BM1629
                     /note="color: black; sequence: ATCGTGGCTCAGCTGAG"
     primer_bind     complement(5312..5323)
                     /label=BM1629
                     /note="color: black; sequence: ATCGTGGCTCAGCTGAG"
     misc_feature    5329..5331
                     /label=Asp > Asn
                     /note="Geneious type: modified base "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5359..5361
                     /label=His > Arg
                     /note="Geneious type: modified base "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5401..5403
                     /label=Asp > Asn
                     /note="Geneious type: modified base "
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(5403..5420)
                     /label=BM1583
                     /note="color: black; sequence: AGTCCCTTCTTCACGGCG"
     primer_bind     5411..5429
                     /label=BM1848
                     /note="color: black; sequence: AGAAGGGACTGGGCGGATC"
     misc_feature    5425..5430
                     /label=linker
                     /note="Geneious type: linker)"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             5431..5520
                     /label=DddA (G1397-C)
                     /note="/created by=bmok"
                     /note="/modified by=bmok"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    5476..5478
                     /label=T1413I
                     /note="Geneious type: modified base"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(5505..5521)
                     /label=BM1944
                     /note="color: black; sequence: AGCAACCGCCTTTGGTC"
     primer_bind     5505..5520
                     /label=BM1971
                     /note="color: black; sequence: GACCAAAGGCGGTTGC"
     primer_bind     5511..5544
                     /label=BM1945
                     /note="color: black; sequence: 
                     AGGCGGTTGCTAATCGACAAGCTTGGCTGTTTTG"
     misc_feature    5521..5523
                     /label=stop
                     /note="Geneious type: STOP"
                     /note="/created by=bmok"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(5568..5585)
                     /label=pBAD Reverse
                     /note="color: black; sequence: TGATTTAATCTGTATCAG"
     primer_bind     complement(5601..5623)
                     /label=BM1972
                     /note="color: black; sequence: CCAGGCAAATTCTGTTTTATCAG"
     terminator      5617..5774
                     /label=rrnB term
                     /note="/Imported from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Plas
                     Mapper</a>"
                     /note="/Transferred From=rrnB term"
                     /note="/Transferred Similarity=100.00%"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     5736..5751
                     /label=BM2049
                     /note="color: black; sequence: AGGGACTCTCAAATAAAACGAAAG"
     terminator      5737..5823
                     /label=rrnB T1 terminator
                     /note="/ApEinfo_fwdcolor=#f8d3a9"
                     /note="/ApEinfo_revcolor=#f8d3a9"
                     /note="color: #ffffff; direction: RIGHT"
     terminator      5740..5783
                     /label=rrnB_T1 term
                     /note="/Imported from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Plas
                     Mapper</a>"
                     /note="/Transferred From=rrnB_T1 term"
                     /note="/Transferred Similarity=97.73%"
                     /note="color: #ffffff; direction: RIGHT"
     terminator      5915..5942
                     /label=rrnB T2 terminator
                     /note="/ApEinfo_fwdcolor=#b4abac"
                     /note="/ApEinfo_revcolor=#b4abac"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(5969..6015)
                     /label=BM1946
                     /note="color: black; sequence: 
                     ACCTAGATCCTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGG"
     primer_bind     6008..6035
                     /label=BM1947
                     /note="color: black; sequence: 
                     ATCTAGGTGAAGATCCTTTTTGATAATC"
     primer_bind     complement(join(6532..6555,1..11))
                     /label=BM1938
                     /note="color: black; sequence: 
                     AGTTTTTCTAATTTTTTTAAGGCAGTTATTGGTGC"
ORIGIN
        1 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
       61 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
      121 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
      181 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
      241 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
      301 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
      361 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
      421 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
      481 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
      541 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
      601 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
      661 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
      721 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
      781 cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
      841 gtaagcagac agttttattg ttcatgaaga gaaacctacg gatcacgtct cattttcgcc
      901 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
      961 agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact
     1021 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
     1081 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat
     1141 cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag
     1201 tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag
     1261 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
     1321 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
     1381 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
     1441 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
     1501 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     1561 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
     1621 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
     1681 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
     1741 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
     1801 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
     1861 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggctgttttt tcgttttcag
     1921 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
     1981 aatatttcta ggctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     2041 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     2101 gcgtagagga tctgctcatg tttgacagct tatcatcgat gcataatgtg cctgtcaaat
     2161 ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt
     2221 cgttaccaaa cttagggact ctcaaataaa acgaaaggct cagtcgaaag actgggcctt
     2281 tcgttttaga cttagggacc ctttatgaca acttgacggc tacatcattc actttttctt
     2341 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
     2401 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
     2461 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
     2521 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
     2581 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
     2641 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
     2701 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
     2761 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
     2821 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
     2881 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
     2941 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
     3001 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
     3061 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
     3121 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
     3181 tacttttcat actcccacca ttcagagaag aaaccaattg tccatattgc atcagacatt
     3241 gccgtcactg cgtcttttac tggctcttct cgctaaccca accggtaacc ccgcttatta
     3301 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
     3361 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
     3421 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
     3481 ctgtttctcc atacccgttt ttttggacgc gtacaactca agtctgacaa aaaaaaggaa
     3541 aaattgacta atctgagcga catcattgag aaggagactg ggaaacagct ggtcattcag
     3601 gagtccatcc tgatgctgcc taaggaggtg aaggaagtga tcggcaacaa gccagagtct
     3661 gacatcctgg tgcacaccgc ctacgacgag tccacagatg agaatgtgat gctgctgacc
     3721 tctgacgccc ccgagtataa gccttgggcc ctggtcatcc aggattctaa cggcgagaat
     3781 aagatcaaga tgctgtctgg tggttctaac atcgccgatc tgcggaccct gggctactcc
     3841 cagcagcagc aggagaagat caagccaaag gtgcgctcta ccgtggctca gcaccacgag
     3901 gccctggtgg gacacggctt cacacacgct cacatcgtgg ccctgtccca gcaccctgcc
     3961 gctctgggca ccgtggccgt gaagtaccag gacatgatcg ccgctctgcc agaggctaca
     4021 cacgaggcca tcgtgggagt gggcaagcag tggtctggag ctcgggctct ggaggctctg
     4081 ctgaccgtgg ctggagagct aagggaacca cctctgcagc tggataccgg ccagctgctg
     4141 aagatcgcca agaggggagg agtgacagct gtggaggctg tgcacgcttg gagaaacgct
     4201 ctgaccggag ctcctctgaa cctgacccca gaacaggttg tggctatcgc ctctcacgac
     4261 ggcggcaaac aggccctgga aaccgtgcag gctctgctgc cagtgctgtg tcaggcccac
     4321 ggcctgaccc ctcagcaggt ggtggccata gccagcaaca acggcggcaa acaggccctg
     4381 gaaaccgtgc agcggctgct gcccgtgctg tgccaagccc acggccttac accacagcag
     4441 gtggtggcta tcgccagcaa cggcggcggc aagcaggctc tggaaaccgt tcagcggctg
     4501 ctgcctgtgc tgtgtcaagc ccacggcctg acccccgagc aggtggtggc catcgcctcc
     4561 cacgacgggg gcaagcaggc cctggagaca gtgcaggccc ttctgcctgt gctgtgccaa
     4621 gcccacggcc tgacacccga gcaggtggtg gccattgctt ctaacaacgg cggaaagcag
     4681 gccctggaga cagtgcaggc cctgctgcct gtgctgtgcc aggctcatgg cctgacccca
     4741 gagcaggtgg tggccatcgc ctccaatgga ggcggcaagc aggccctgga aaccgtgcag
     4801 gccctgctgc ctgtgctgtg ccaggcccac ggactgacac ccgagcaagt ggtcgccatc
     4861 gctagcaacg gcggcggaaa gcaggctctg gaaaccgttc aaagactgct gcccgtgctg
     4921 tgccaggctc acggcctgac ccctgagcag gtcgtggcca tcgccagcaa tggcggaggc
     4981 aagcaggctc tggagacagt gcaggccctg ctgcccgtcc tgtgccaggc ccatggcctc
     5041 acccctgagc aggtggtggc cattgccagc aacaacggcg gaaagcaggc cctggaaacc
     5101 gtgcagagac tgctgcctgt gctgtgccag gcacacggcc tgacccctga gcaggtggtg
     5161 gccatcgcca gccacgatgg cggaaagcaa gctctggaaa ccgtgcaggc cctgctgccc
     5221 gtgctgtgtc aggcccacgg cctgacacct caacaagtag ttgccatcgc cagcaacggc
     5281 ggcggtagac ctgccctcga gtctatcgtg gctcagctga gccgcccaaa ccccgccctg
     5341 gccgctctga ccaacgatcg cctggtggcc ctggcttgtc tgggaggccg gcctgctctg
     5401 aacgccgtga agaagggact gggcggatcc gccattccag tgaagcgcgg cgctaccggt
     5461 gaaaccaaag tgtttatcgg taacagcaac agcccgaaga gcccgaccaa aggcggttgc
     5521 taatcgacaa gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
     5581 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg
     5641 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
     5701 ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
     5761 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     5821 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
     5881 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
     5941 tttgcgtttc tacaaactct ctgtcagacc aagtttactc atatatactt tagattgatt
     6001 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6061 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6121 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6181 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6241 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6301 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtcaggc atttgagaag
     6361 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
     6421 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
     6481 cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata
     6541 actgccttaa aaaaa
//
