LOCUS       pH6-MBP-BirA            7605 bp ds-DNA     circular     11-MAR-2021
DEFINITION  .
KEYWORDS    "accession:addgene_75286_142418"                                   
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     promoter        3..80
                     /label="lacIq promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             81..1163
                     /label="lacI"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(100..119)
                     /label="LacI-R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        1406..1434
                     /label="tac promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    1442..1458
                     /label="lac operator"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     1447..1469
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1528..2691
                     /label="Translation 1528-2691"
     CDS             1528..3687
                     /label="Translation 1528-3687"
     misc_feature    1540..1557
                     /label="His6 tag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1564..1584
                     /label="Thrombin site"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1588..2691
                     /label="MBP"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2600..2617
                     /label="pMA510.Seq.Primer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(2673..2691)
                     /label="pH6.MAL.Vec.Rev."
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2674..3708
                     /label="BirA.gBlock"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             2692..2718
                     /label="Translation 2692-2718"
     CDS             2719..3687
                     /label="Translation 2719-3687"
     misc_feature    2722..3687
                     /label="BirA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3688..3692
                     /label="HindIII"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(3696..3712)
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(3696..3713)
                     /label="M13 Forward"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(3705..3727)
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(3891..3908)
                     /label="pBAD Reverse"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      4061..4147
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      4239..4266
                     /label="rrnB T2 terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        4285..4376
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             4377..5237
                     /label="AmpR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(4595..4614)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(5279..5792)
                     /label="M13 ori"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(5474..5495)
                     /label="F1ori-F"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     5686..5705
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      5903..6491
                     /label="ori"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     6392..6411
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     6645..6662
                     /label="L4440"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    6677..6819
                     /label="bom"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(6745..6764)
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     6905..6927
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(6921..7112)
                     /label="rop"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagcatATG GGCAGCAGCC ATCATCATCA TCATCACAGC
     1561 AGCGGCCTGG TGCCGCGCGG CAGCCATATG AAAATCGAAG AAGGTAAACT GGTAATCTGG
     1621 ATTAACGGCG ATAAAGGCTA TAACGGTCTC GCTGAAGTCG GTAAGAAATT CGAGAAAGAT
     1681 ACCGGAATTA AAGTCACCGT TGAGCATCCG GATAAACTGG AAGAGAAATT CCCACAGGTT
     1741 GCGGCAACTG GCGATGGCCC TGACATTATC TTCTGGGCAC ACGACCGCTT TGGTGGCTAC
     1801 GCTCAATCTG GCCTGTTGGC TGAAATCACC CCGGACAAAG CGTTCCAGGA CAAGCTGTAT
     1861 CCGTTTACCT GGGATGCCGT ACGTTACAAC GGCAAGCTGA TTGCTTACCC GATCGCTGTT
     1921 GAAGCGTTAT CGCTGATTTA TAACAAAGAT CTGCTGCCGA ACCCGCCAAA AACCTGGGAA
     1981 GAGATCCCGG CGCTGGATAA AGAACTGAAA GCGAAAGGTA AGAGCGCGCT GATGTTCAAC
     2041 CTGCAAGAAC CGTACTTCAC CTGGCCGCTG ATTGCTGCTG ACGGGGGTTA TGCGTTCAAG
     2101 TATGAAAACG GCAAGTACGA CATTAAAGAC GTGGGCGTGG ATAACGCTGG CGCGAAAGCG
     2161 GGTCTGACCT TCCTGGTTGA CCTGATTAAA AACAAACACA TGAATGCAGA CACCGATTAC
     2221 TCCATCGCAG AAGCTGCCTT TAATAAAGGC GAAACAGCGA TGACCATCAA CGGCCCGTGG
     2281 GCATGGTCCA ACATCGACAC CAGCAAAGTG AATTATGGTG TAACGGTACT GCCGACCTTC
     2341 AAGGGTCAAC CATCCAAACC GTTCGTTGTC GTGCTGAGCG CAGGTATTAA CGCCGCCAGT
     2401 CCGAACAAAG AGCTGGCAAA AGAGTTCCTC GAAAACTATC TGCTGACTGA TGAAGGTCTG
     2461 GAAGCGGTTA ATAAAGACAA ACCGCTGGGT GCCGTAGCGC TGAAGTCTTA CGAGGAAGAG
     2521 TTGGCGAAAG ATCCACGTAT TGCCGCCACT ATGGAAAACG CCCAGAAAGG TGAAATCATG
     2581 CCGAACATCC CGCAGATGTC CGCTTTCTGG TATGCCGTGC GTACTGCGGT GATCAACGCC
     2641 GCCAGCGGTC GTCAGACTGT CGATGAAGCC CTGAAAGACG CGCAGACTAA TTCGAGCTCA
     2701 GAGAACCTGT ATTTCCAGGG AAAGGATAAC ACCGTGCCAC TGAAATTGAT TGCCCTGTTA
     2761 GCGAACGGTG AATTTCACTC TGGCGAGCAG TTGGGTGAAA CGCTGGGAAT GAGCCGGGCG
     2821 GCTATTAATA AACACATTCA GACACTGCGT GACTGGGGCG TTGATGTCTT TACCGTTCCG
     2881 GGTAAAGGAT ACAGCCTGCC TGAGCCTATC CAGTTACTTA ATGCTAAACA GATATTGGGT
     2941 CAGCTGGATG GCGGTAGTGT AGCCGTGCTG CCAGTGATTG ACTCCACGAA TCAGTACCTT
     3001 CTTGATCGTA TCGGAGAGCT TAAATCGGGC GATGCTTGCA TTGCAGAATA CCAGCAGGCT
     3061 GGCCGTGGTC GCCGGGGTCG GAAATGGTTT TCGCCTTTTG GCGCAAACTT ATATTTGTCG
     3121 ATGTTCTGGC GTCTGGAACA AGGCCCGGCG GCGGCGATTG GTTTAAGTCT GGTTATCGGT
     3181 ATCGTGATGG CGGAAGTATT ACGCAAGCTG GGTGCAGATA AAGTTCGTGT TAAATGGCCT
     3241 AATGACCTCT ATCTGCAGGA TCGCAAGCTG GCAGGCATTC TGGTGGAGCT GACTGGCAAA
     3301 ACTGGCGATG CGGCGCAAAT AGTCATTGGA GCCGGGATCA ACATGGCAAT GCGCCGTGTT
     3361 GAAGAGAGTG TCGTTAATCA GGGGTGGATC ACGCTGCAGG AAGCGGGGAT CAATCTCGAT
     3421 CGTAATACGT TGGCGGCCAT GCTAATACGT GAATTACGTG CTGCGTTGGA ACTCTTCGAA
     3481 CAAGAAGGAT TGGCACCTTA TCTGTCGCGC TGGGAAAAGC TGGATAATTT TATTAATCGC
     3541 CCAGTGAAAC TTATCATTGG TGATAAAGAA ATATTTGGCA TTTCACGCGG AATAGACAAA
     3601 CAGGGGGCTT TATTACTTGA GCAGGATGGA ATAATAAAAC CCTGGATGGG CGGTGAAATA
     3661 TCCCTGCGTA GTGCAGAAAA ATAATGAagc ttggcactgg ccgtcgtttt acaacgtcgt
     3721 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3781 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3841 aatggcgaat ggcagcttgg ctgttttggc ggatgagata agattttcag cctgatacag
     3901 attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg
     3961 gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt
     4021 gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
     4081 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     4141 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
     4201 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg
     4261 cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc
     4321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4561 aacgtttccc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     4621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4921 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttac
     5281 cccggttgat aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat
     5341 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     5401 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     5461 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     5521 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc
     5581 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     5641 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     5701 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     5761 cgccgcgctt aatgcgccgc tacagggcgc gtaaaaggat ctaggtgaag atcctttttg
     5821 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5881 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5941 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6001 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     6061 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6121 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6181 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6241 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6301 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6361 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6421 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6481 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6541 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6601 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6661 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6721 accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     6781 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     6841 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     6901 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     6961 ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac agatgtctgc ctgttcatcc
     7021 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     7081 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     7141 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     7201 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     7261 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
     7321 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
     7381 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     7441 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     7501 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     7561 acgatcatgc gcacccgtgg ccaggaccca acgctgcccg aaatt
//