LOCUS       Exported               11712 bp DNA     circular SYN 17-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Jason Vevea
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     primer_bind     2582..2624
                     /label=NC32 For IF
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(3030..3065)
                     /label=NC32 Rev IF
     misc_feature    3086..4294
                     /label=Syt7a
     misc_feature    3134..3196
                     /label=Syt7 TMD
     misc_feature    4295..4336
                     /label=GS(GSS)4 Linker 1
     CDS             4337..4357
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             4373..5254
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     CDS             5267..5323
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5324..5377
                     /codon_start=1
                     /label=BiP signal sequence (alt codons)
                     /translation="MKLSLVAAMLLLLSAARA"
     CDS             5411..5509
                     /codon_start=1
                     /label=CD4 TMD (alt codons)
                     /translation="VLPTWSTPVQPMALIVLGGVAGLLLFIGLGIFF"
     misc_feature    5444..5509
                     /label=TMD
     CDS             5510..5545
                     /codon_start=1
                     /product="Tribasic golgi export signal KRRERRR
                     Parmar, H.B., Barry, C., Kai, F. and Duncan, R., 2014. 
                     Golgi complex?plasma membrane trafficking directed by an 
                     autonomous, tribasic Golgi export signal. Molecular biology
                     of the cell, 25(6), pp.866-878."
                     /label=Golgi Export signal
                     /translation="KYLQKRRERRRQ"
     misc_feature    5546..5587
                     /label=GS(GSS)4 Linker 3
     CDS             5588..6283
                     /codon_start=1
                     /label=msGFP
                     /translation="MDSTESLFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTITFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLG"
     primer_bind     5588..5623
                     /label=DSTES
                     /note="Use together with BL14 to amplify msGFP, msYFP, 
                     andmsCFP with the improved N-terminal codons from A24. 
                     Subclone the PCR product back into the same vector using 
                     BamHI and NotI."
     primer_bind     5591..5614
                     /label=new N GFP lin F
     primer_bind     complement(6266..6289)
                     /label=BL14
                     /note="This oligo (used with BL13) will replace the linker 
                     MDELYK to GSSGSS in monomeric superfolder GFP, CFP and 
                     YFP."
     primer_bind     complement(6273..6289)
                     /label=BL24
                     /note="This oligo is used to amplify msfFPs (Green, Cyan, 
                     Yellow) from the pMSGFP vector into the NT-HT vector for 
                     the Tub1 fussion.  The cut sites are BspEI for the 
                     N-terminal and SacI for the C-terminal. Use this oligo with
                     BL23."
     CDS             6293..6316
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6317..6358
                     /codon_start=1
                     /product="(FxYENEV) in the cytoplasmic C-terminus from
                     mammalian Kir2.1 channels
                     Stockklausner, C., Ludwig, J., Ruppersberg, J.P. and 
                     Klocker, N., 2001. A sequence motif responsible for ER 
                     export and surface expression of Kir2. 0 inward rectifier 
                     K+ channels. FEBS letters, 493(2-3), pp.129-133."
                     /label=ER export signal
                     /translation="ENANSFCYENEVAL"
     misc_feature    6404..6992
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(6875..6886)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6995..7011)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7517..7697
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7729..7953
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7999..8427
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8441..8770
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8621..8756
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8818..8865
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8884..9258
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9388..9509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9558..9574)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9582..9598
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9606..9636)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9651..9672
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9960..10548)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10719..11579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11580..11684)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctagcg
     3061 gatccggtac cgcgggcccg ccaccatgta ccgggacccg gaggcggcca gcccaggggc
     3121 acctacccgc gatgtcctgc tggtctctgc aatcatcacc gtcagcctta gcgtcactat
     3181 cgtcctctgc ggcctgtgcc actggtgtca gcgcaaactg ggcaaacgct acaagaattc
     3241 cttggagacg gtgggcacgc cagactcggg gcgtgggcgc ggtgagaaga aagccatcaa
     3301 gttgcctgca ggagggaagg ctgtgaatac agccccagtg cccggccaga cgccacacga
     3361 tgagtctgac cgcagaacgg agacccgttc ctctgtctcg gacctcgtca actcccttac
     3421 cagcgagatg ctcatgctct ccccgggttc tgaggaggat gaggcccacg agggctgcag
     3481 ccgagagaac ctgggccgaa tccagttcag tgttggctac aacttccaag agtccacact
     3541 caccgtgaag gtcatgaagg cccaagagct gccagccaag gacttcagcg gtactagtga
     3601 cccctttgtc aagatctacc tgctacccga caagaagcac aaactggaga ccaaggtgaa
     3661 gcggaagaat ctaaacccgc actggaatga gacctttcta tttgaagggt ttccctacga
     3721 gaaagtggtg cagagggtcc tctacctcca ggtcctggat tatgaccgtt tcagccgcaa
     3781 tgaccccatt ggggaggtgt ccatccctct gaacaaggtg gacctgaccc agatgcagac
     3841 cttctggaag gatctgaagc catgcagcga tgggagtggg agccgagggg agctgctctt
     3901 gtccctctgc tacaacccct ctgccaactc catcatcgtg aacatcatca aagctcgaaa
     3961 cctcaaagcc atggacatcg ggggcacatc agacccctat gtgaaggtgt ggctgatgta
     4021 taaagacaag cgggtagaga aaaagaagac cgtgacaaag aagaggaacc tgaaccccat
     4081 cttcaatgag tctttcgcct tcgacatacc cacggagaag ctgagggaga ccacgatcat
     4141 catcactgtc atggacaaag acaagctcag ccgcaacgac gtcatcggca agatctacct
     4201 gtcctggaag agcggaccag gtgaagtgaa acactggaag gacatgatcg ctcgtccccg
     4261 gcagcctgtg gcccagtggc accagctgaa agccggatcc ggaagttcgg ggtccagcgg
     4321 ttcttcaggc agttccgagg atctgtactt tcagagcgat aacgatggat ccgaaatcgg
     4381 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     4441 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     4501 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     4561 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     4621 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     4681 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     4741 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     4801 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     4861 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     4921 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     4981 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     5041 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     5101 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     5161 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     5221 gatcgcgcgc tggctgtcta ctctggagat ttccggtggt agcggggcca ctaatttttc
     5281 attgcttaaa caagctgggg acgtggagga gaacccaggc cccatgaagc tctctttggt
     5341 ggctgcaatg ctgcttctgc tcagtgctgc gagggctagc ggtggaggtg gaagtggagg
     5401 tggaggatct gttctgccca catggtctac acctgttcag ccaatggccc tgattgtgct
     5461 ggggggcgtc gccggcctcc tgcttttcat tgggctaggc atcttcttca agtacctgca
     5521 gaaaagaagg gagcggagac gccaggggtc aggttcttcg ggcagttcgg ggtccagcgg
     5581 atctagtatg gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct
     5641 ggacggcgac gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac
     5701 caacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     5761 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     5821 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     5881 caccttcaag gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac
     5941 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     6001 gcacaagctg gagtacaact tcaacagcca caacgtctat atcaccgccg acaagcagaa
     6061 gaacggcatc aaggccaact tcaagatccg ccacaacgtg gaggacggca gcgtgcagct
     6121 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     6181 ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     6241 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcggatcca gtgactacaa
     6301 ggatgacgac gataaagaga acgcaaacag cttttgctat gaaaatgaag ttgccctcta
     6361 aggccgcact cctcagctag aattcgatat caagcttatc gataatcaac ctctggatta
     6421 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6481 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6541 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6601 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6661 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6721 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6781 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     6841 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6901 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6961 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga
     7021 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     7081 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     7141 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7201 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     7261 aggctacttc cctgattggc agaactacac accagggcca gggatcagat atccactgac
     7321 ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga
     7381 aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     7441 agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct
     7501 gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7561 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7621 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7681 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     7741 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     7801 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     7861 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     7921 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     7981 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     8041 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     8101 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     8161 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     8221 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     8281 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     8341 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     8401 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     8461 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8521 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8581 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8641 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8701 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8761 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     8821 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     8881 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     8941 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     9001 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     9061 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     9121 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     9181 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     9241 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     9301 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     9361 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     9421 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9481 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     9541 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9601 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     9661 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9721 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9781 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9841 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9901 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9961 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    10021 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    10081 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    10141 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    10201 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    10261 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    10321 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    10381 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    10441 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10501 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10561 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10621 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10681 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10741 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10801 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10861 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10921 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10981 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    11041 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    11101 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    11161 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    11221 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    11281 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    11341 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    11401 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    11461 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    11521 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11581 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    11641 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    11701 gtgccacctg ac
//
