LOCUS       Exported               10488 bp DNA     circular SYN 06-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Jason Vevea
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     primer_bind     2582..2624
                     /label=NC32 For IF
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(3030..3065)
                     /label=NC32 Rev IF
     regulatory      3080..3089
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3086..4009
                     /codon_start=1
                     /label=SYP (Synaptophysin)
                     /translation="MDVVNQLVAGGQFRVVKEPLGFVKVLQWVFAIFAFATCGSYTGEL
                     RLSVECANKTESALNIEVEFEYPFRLHQVYFDAPSCVKGGTTKIFLVGDYSSSAEFFVT
                     VAVFAFLYSMGALATYIFLQNKYRENNKGPMMDFLATAVFAFMWLVSSSAWAKGLSDVK
                     MATDPENIIKEMPMCRQTGNTCKELRDPVTSGLNTSVVFGFLNLVLWVGNLWFVFKETG
                     WAAPFMRAPPGAPEKQPAPGDAYGDAGYGQGPGGYGPQDSYGPQGGYQPDYGQPASGGG
                     GYGPQGDYGQQGYGQQGAPTSFSNQMK"
     misc_feature    3143..3214
                     /label=SYP TMD1
     misc_feature    4010..4051
                     /label=GS(GSS)4 Linker 1
     misc_feature    4052..5134
                     /label=Syt7a Delta-TMD
     misc_feature    4334..4642
                     /label=Syt7 C2A
     misc_feature    4418..4441
                     /label=Syt7 C2A Loop 1
     misc_feature    4499..4519
                     /label=Syt7 C2A Loop 2
     misc_feature    4598..4624
                     /label=Syt7 C2A Loop 3
     misc_feature    4718..5086
                     /label=S7 C2B
                     /note="C2B region demarcated based on Fig 4 of (Sudhof and
                     Rizo 1996)"
     misc_feature    4802..4834
                     /label=S7 C2B Loop 1
     misc_feature    4898..4918
                     /label=S7 C2B Loop 2
     misc_feature    4994..5020
                     /label=S7 C2B Loop 3
     misc_feature    5180..5768
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(5651..5662)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5771..5787)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6293..6473
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6505..6729
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6775..7203
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7217..7546
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7397..7532
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7594..7641
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7660..8034
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8164..8285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8334..8350)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8358..8374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8382..8412)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8427..8448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8736..9324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9495..10355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10356..10460)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctagcg
     3061 gatccggtac cgcgggcccg ccaccatgga cgtggtgaat cagctggtgg ctgggggtca
     3121 gttccgggtg gtcaaggagc cccttggctt cgtgaaggtg ctgcagtggg tctttgccat
     3181 cttcgccttt gctacgtgtg gcagctacac cggggagctt cggctgagcg tggagtgtgc
     3241 caacaagacg gagagtgccc tcaacatcga agttgaattc gagtacccct tcaggctgca
     3301 ccaagtgtac tttgatgcac cctcctgcgt caaagggggc actaccaaga tcttcctggt
     3361 tggggactac tcctcgtcgg ctgaattctt tgtcaccgtg gctgtgtttg ccttcctcta
     3421 ctccatgggg gccctggcca cctacatctt cctgcagaac aagtaccgag agaacaacaa
     3481 agggcctatg atggactttc tggctacagc cgtgttcgct ttcatgtggc tagttagttc
     3541 atcagcctgg gccaaaggcc tgtccgatgt gaagatggcc acggacccag agaacattat
     3601 caaggagatg cccatgtgcc gccagacagg gaacacatgc aaggaactga gggaccctgt
     3661 gacttcagga ctcaacacct cagtggtgtt tggcttcctg aacctggtgc tctgggttgg
     3721 caacttatgg ttcgtgttca aggagacagg ctgggcagcc ccattcatgc gcgcacctcc
     3781 aggcgccccg gaaaagcaac cagcacctgg cgatgcctac ggcgatgcgg gctacgggca
     3841 gggccccgga ggctatgggc cccaggactc ctacgggcct cagggtggtt atcaacccga
     3901 ttacgggcag ccagccagcg gtggcggtgg ctacgggcct cagggcgact atgggcagca
     3961 aggctatggc caacagggtg cgcccacctc cttctccaat cagatgaaag gatccggaag
     4021 ttcggggtcc agcggttctt caggcagttc ccgcaaactg ggcaaacgct acaagaattc
     4081 cttggagacg gtgggcacgc cagactcggg gcgtgggcgc ggtgagaaga aagccatcaa
     4141 gttgcctgca ggagggaagg ctgtgaatac agccccagtg cccggccaga cgccacacga
     4201 tgagtctgac cgcagaacgg agacccgttc ctctgtctcg gacctcgtca actcccttac
     4261 cagcgagatg ctcatgctct ccccgggttc tgaggaggat gaggcccacg agggctgcag
     4321 ccgagagaac ctgggccgaa tccagttcag tgttggctac aacttccaag agtccacact
     4381 caccgtgaag gtcatgaagg cccaagagct gccagccaag gacttcagcg gtactagtga
     4441 cccctttgtc aagatctacc tgctacccga caagaagcac aaactggaga ccaaggtgaa
     4501 gcggaagaat ctaaacccgc actggaatga gacctttcta tttgaagggt ttccctacga
     4561 gaaagtggtg cagagggtcc tctacctcca ggtcctggat tatgaccgtt tcagccgcaa
     4621 tgaccccatt ggggaggtgt ccatccctct gaacaaggtg gacctgaccc agatgcagac
     4681 cttctggaag gatctgaagc catgcagcga tgggagtggg agccgagggg agctgctctt
     4741 gtccctctgc tacaacccct ctgccaactc catcatcgtg aacatcatca aagctcgaaa
     4801 cctcaaagcc atggacatcg ggggcacatc agacccctat gtgaaggtgt ggctgatgta
     4861 taaagacaag cgggtagaga aaaagaagac cgtgacaaag aagaggaacc tgaaccccat
     4921 cttcaatgag tctttcgcct tcgacatacc cacggagaag ctgagggaga ccacgatcat
     4981 catcactgtc atggacaaag acaagctcag ccgcaacgac gtcatcggca agatctacct
     5041 gtcctggaag agcggaccag gtgaagtgaa acactggaag gacatgatcg ctcgtccccg
     5101 gcagcctgtg gcccagtggc accagctgaa agcctaaggc cgcactcctc agctagaatt
     5161 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     5221 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5281 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     5341 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     5401 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5461 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5521 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     5581 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5641 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     5701 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5761 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     5821 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     5881 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     5941 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6001 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     6061 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     6121 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     6181 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     6241 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     6301 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6361 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6421 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     6481 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6541 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6601 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6661 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     6721 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     6781 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6841 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6901 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6961 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7021 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7081 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7141 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7201 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7261 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     7321 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     7381 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7441 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     7501 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     7561 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     7621 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     7681 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     7741 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     7801 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     7861 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     7921 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     7981 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     8041 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     8101 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     8161 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8221 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8281 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8341 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8401 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8461 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8521 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8581 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8641 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8701 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8761 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8821 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8881 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8941 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9001 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9061 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9121 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9181 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9241 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9301 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9361 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9421 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9481 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9541 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9601 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9661 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9721 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9781 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9841 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9901 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9961 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10021 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10081 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10141 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10201 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10261 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10321 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10381 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10441 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgac
//
