LOCUS       Exported               11451 bp DNA     circular SYN 10-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Jason Vevea
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     primer_bind     2582..2624
                     /label=NC32 For IF
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(3030..3065)
                     /label=NC32 Rev IF
     regulatory      3080..3089
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3086..4009
                     /codon_start=1
                     /label=SYP (Synaptophysin)
                     /translation="MDVVNQLVAGGQFRVVKEPLGFVKVLQWVFAIFAFATCGSYTGEL
                     RLSVECANKTESALNIEVEFEYPFRLHQVYFDAPSCVKGGTTKIFLVGDYSSSAEFFVT
                     VAVFAFLYSMGALATYIFLQNKYRENNKGPMMDFLATAVFAFMWLVSSSAWAKGLSDVK
                     MATDPENIIKEMPMCRQTGNTCKELRDPVTSGLNTSVVFGFLNLVLWVGNLWFVFKETG
                     WAAPFMRAPPGAPEKQPAPGDAYGDAGYGQGPGGYGPQDSYGPQGGYQPDYGQPASGGG
                     GYGPQGDYGQQGYGQQGAPTSFSNQMK"
     misc_feature    3143..3214
                     /label=SYP TMD1
     misc_feature    4010..4051
                     /label=GS(GSS)4 Linker 3
     misc_feature    4052..5134
                     /label=Syt7a Delta-TMD
     misc_feature    4334..4642
                     /label=Syt7 C2A
     misc_feature    4418..4441
                     /label=Syt7 C2A Loop 1
     misc_feature    4499..4519
                     /label=Syt7 C2A Loop 2
     misc_feature    4598..4624
                     /label=Syt7 C2A Loop 3
     misc_feature    4718..5086
                     /label=S7 C2B
                     /note="C2B region demarcated based on Fig 4 of (Sudhof and
                     Rizo 1996)"
     misc_feature    4802..4834
                     /label=S7 C2B Loop 1
     misc_feature    4898..4918
                     /label=S7 C2B Loop 2
     misc_feature    4994..5020
                     /label=S7 C2B Loop 3
     misc_feature    5135..5176
                     /label=GS(GSS)4 Linker 1
     CDS             5177..5197
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             5213..6094
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     misc_feature    6143..6731
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(6614..6625)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6734..6750)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7256..7436
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7468..7692
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7738..8166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8180..8509
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8360..8495
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8557..8604
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8623..8997
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9127..9248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9297..9313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9321..9337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9345..9375)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9390..9411
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9699..10287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10458..11318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11319..11423)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctagcg
     3061 gatccggtac cgcgggcccg ccaccatgga cgtggtgaat cagctggtgg ctgggggtca
     3121 gttccgggtg gtcaaggagc cccttggctt cgtgaaggtg ctgcagtggg tctttgccat
     3181 cttcgccttt gctacgtgtg gcagctacac cggggagctt cggctgagcg tggagtgtgc
     3241 caacaagacg gagagtgccc tcaacatcga agttgaattc gagtacccct tcaggctgca
     3301 ccaagtgtac tttgatgcac cctcctgcgt caaagggggc actaccaaga tcttcctggt
     3361 tggggactac tcctcgtcgg ctgaattctt tgtcaccgtg gctgtgtttg ccttcctcta
     3421 ctccatgggg gccctggcca cctacatctt cctgcagaac aagtaccgag agaacaacaa
     3481 agggcctatg atggactttc tggctacagc cgtgttcgct ttcatgtggc tagttagttc
     3541 atcagcctgg gccaaaggcc tgtccgatgt gaagatggcc acggacccag agaacattat
     3601 caaggagatg cccatgtgcc gccagacagg gaacacatgc aaggaactga gggaccctgt
     3661 gacttcagga ctcaacacct cagtggtgtt tggcttcctg aacctggtgc tctgggttgg
     3721 caacttatgg ttcgtgttca aggagacagg ctgggcagcc ccattcatgc gcgcacctcc
     3781 aggcgccccg gaaaagcaac cagcacctgg cgatgcctac ggcgatgcgg gctacgggca
     3841 gggccccgga ggctatgggc cccaggactc ctacgggcct cagggtggtt atcaacccga
     3901 ttacgggcag ccagccagcg gtggcggtgg ctacgggcct cagggcgact atgggcagca
     3961 aggctatggc caacagggtg cgcccacctc cttctccaat cagatgaaag ggtcaggttc
     4021 ttcgggcagt tcggggtcca gcggatctag tcgcaaactg ggcaaacgct acaagaattc
     4081 cttggagacg gtgggcacgc cagactcggg gcgtgggcgc ggtgagaaga aagccatcaa
     4141 gttgcctgca ggagggaagg ctgtgaatac agccccagtg cccggccaga cgccacacga
     4201 tgagtctgac cgcagaacgg agacccgttc ctctgtctcg gacctcgtca actcccttac
     4261 cagcgagatg ctcatgctct ccccgggttc tgaggaggat gaggcccacg agggctgcag
     4321 ccgagagaac ctgggccgaa tccagttcag tgttggctac aacttccaag agtccacact
     4381 caccgtgaag gtcatgaagg cccaagagct gccagccaag gacttcagcg gtactagtga
     4441 cccctttgtc aagatctacc tgctacccga caagaagcac aaactggaga ccaaggtgaa
     4501 gcggaagaat ctaaacccgc actggaatga gacctttcta tttgaagggt ttccctacga
     4561 gaaagtggtg cagagggtcc tctacctcca ggtcctggat tatgaccgtt tcagccgcaa
     4621 tgaccccatt ggggaggtgt ccatccctct gaacaaggtg gacctgaccc agatgcagac
     4681 cttctggaag gatctgaagc catgcagcga tgggagtggg agccgagggg agctgctctt
     4741 gtccctctgc tacaacccct ctgccaactc catcatcgtg aacatcatca aagctcgaaa
     4801 cctcaaagcc atggacatcg ggggcacatc agacccctat gtgaaggtgt ggctgatgta
     4861 taaagacaag cgggtagaga aaaagaagac cgtgacaaag aagaggaacc tgaaccccat
     4921 cttcaatgag tctttcgcct tcgacatacc cacggagaag ctgagggaga ccacgatcat
     4981 catcactgtc atggacaaag acaagctcag ccgcaacgac gtcatcggca agatctacct
     5041 gtcctggaag agcggaccag gtgaagtgaa acactggaag gacatgatcg ctcgtccccg
     5101 gcagcctgtg gcccagtggc accagctgaa agccggatcc ggaagttcgg ggtccagcgg
     5161 ttcttcaggc agttccgagg atctgtactt tcagagcgat aacgatggat ccgaaatcgg
     5221 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     5281 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     5341 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     5401 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     5461 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     5521 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     5581 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     5641 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     5701 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     5761 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     5821 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     5881 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     5941 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     6001 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     6061 gatcgcgcgc tggctgtcta ctctggagat ttccggttaa ggccgcactc ctcagctaga
     6121 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     6181 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6241 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6301 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6361 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6421 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6481 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     6541 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6601 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6661 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6721 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     6781 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6841 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     6901 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6961 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     7021 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     7081 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     7141 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     7201 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     7261 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7321 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7381 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     7441 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     7501 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     7561 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     7621 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     7681 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     7741 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7801 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7861 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7921 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7981 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8041 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8101 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8161 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8221 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8281 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8341 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8401 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     8461 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     8521 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     8581 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     8641 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     8701 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     8761 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     8821 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     8881 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     8941 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     9001 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     9061 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     9121 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9181 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9241 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     9301 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9361 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9421 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9481 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9541 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9601 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9661 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9721 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9781 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9841 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9901 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9961 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10021 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10081 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10141 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10201 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10261 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10321 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10381 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10441 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10501 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10561 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10621 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10681 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10741 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10801 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10861 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10921 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10981 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    11041 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11101 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11161 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11221 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11281 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11341 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga c
//
