LOCUS       Exported               11706 bp DNA     circular SYN 21-SEP-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Jason Vevea
  TITLE     Direct Submission
  JOURNAL   Exported Dec 1, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     primer_bind     2582..2624
                     /label=NC32 For IF
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(3030..3065)
                     /label=NC32 Rev IF
     misc_feature    3086..4306
                     /label=LAMP1
     misc_feature    4307..5389
                     /label=Syt7a
     misc_feature    5390..5431
                     /label=GS(GSS)4 Linker
     CDS             5432..5452
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             5468..6349
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     misc_feature    6398..6986
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(6869..6880)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6989..7005)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7511..7691
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7723..7947
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7993..8421
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8435..8764
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8615..8750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8812..8859
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8878..9252
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9382..9503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9552..9568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9576..9592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9600..9630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9645..9666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9954..10542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10713..11573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11574..11678)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctagcg
     3061 gatccggtac cgcgggcccg ccaccatggc ggccccgggc gcccggcggc cgctgctcct
     3121 gttgctgctg gcaggccttg cacacagcgc cccagcactg ttcgaggtga aagacaacaa
     3181 cggcacagcg tgtataatgg ccagcttctc tgcctccttt ctgaccacct atgatgctgg
     3241 acatgtttct aaggtctcga atatgaccct gccagcctct gcagaagtcc tgaagaatag
     3301 cagctcttgt ggtgaaaaga atgcttctga gcccaccctc gcaatcacct ttggagaagg
     3361 atatttactg aaactcacct tcacaaaaaa cacaacacgt tacagtgtcc agcacatgta
     3421 tttcacatat aacctgtcag acacacaatt ctttcccaat gccagctcca aagggcccga
     3481 cactgtggat tccacaactg acatcaaggc agacatcaac aaaacatacc gatgtgtcag
     3541 cgacatcagg gtctacatga agaatgtgac cattgtgctc tgggacgcta ctatccaggc
     3601 ctacctgccg agtagcaact tcagcaagga agagacacgc tgcccacagg atcaaccttc
     3661 cccaactact gggccaccca gcccctcacc accacttgtg cccacaaacc ccagtgtgtc
     3721 caagtacaat gtgactggtg acaatggaac ctgcctgctg gcctctatgg cactgcaact
     3781 caacatcacc tacatgaaga aggacaacac gactgtgacc agagcattca acatcaaccc
     3841 aagtgacaaa tatagtggga cttgcggtgc ccagttggtg accctgaagg tggggaacaa
     3901 gagcagagtc ctggagctgc agtttgggat gaatgccact tctagcctgt ttttcctgca
     3961 aggagttcag ttgaacatga ctcttcctga tgccatagag cccacgttca gcacctccaa
     4021 ctattccctg aaagctcttc aggccagtgt cggcaactca tacaagtgca acagtgagga
     4081 gcacatcttt gtcagcaagg cgctcgccct caatgtcttc agcgtgcaag tccaggcttt
     4141 cagggtagaa agtgacaggt ttgggtctgt ggaagagtgt gtacaggacg gtaacaacat
     4201 gctgatcccc attgctgtgg gcggggccct ggcagggctg gtcctcatcg tcctcatcgc
     4261 ctacctcatc ggcaggaaga ggagtcacgc gggctatcag accatccgca aactgggcaa
     4321 acgctacaag aattccttgg agacggtggg cacgccagac tcggggcgtg ggcgcggtga
     4381 gaagaaagcc atcaagttgc ctgcaggagg gaaggctgtg aatacagccc cagtgcccgg
     4441 ccagacgcca cacgatgagt ctgaccgcag aacggagacc cgttcctctg tctcggacct
     4501 cgtcaactcc cttaccagcg agatgctcat gctctccccg ggttctgagg aggatgaggc
     4561 ccacgagggc tgcagccgag agaacctggg ccgaatccag ttcagtgttg gctacaactt
     4621 ccaagagtcc acactcaccg tgaaggtcat gaaggcccaa gagctgccag ccaaggactt
     4681 cagcggtact agtgacccct ttgtcaagat ctacctgcta cccgacaaga agcacaaact
     4741 ggagaccaag gtgaagcgga agaatctaaa cccgcactgg aatgagacct ttctatttga
     4801 agggtttccc tacgagaaag tggtgcagag ggtcctctac ctccaggtcc tggattatga
     4861 ccgtttcagc cgcaatgacc ccattgggga ggtgtccatc cctctgaaca aggtggacct
     4921 gacccagatg cagaccttct ggaaggatct gaagccatgc agcgatggga gtgggagccg
     4981 aggggagctg ctcttgtccc tctgctacaa cccctctgcc aactccatca tcgtgaacat
     5041 catcaaagct cgaaacctca aagccatgga catcgggggc acatcagacc cctatgtgaa
     5101 ggtgtggctg atgtataaag acaagcgggt agagaaaaag aagaccgtga caaagaagag
     5161 gaacctgaac cccatcttca atgagtcttt cgccttcgac atacccacgg agaagctgag
     5221 ggagaccacg atcatcatca ctgtcatgga caaagacaag ctcagccgca acgacgtcat
     5281 cggcaagatc tacctgtcct ggaagagcgg accaggtgaa gtgaaacact ggaaggacat
     5341 gatcgctcgt ccccggcagc ctgtggccca gtggcaccag ctgaaagccg gatccggaag
     5401 ttcggggtcc agcggttctt caggcagttc cgaggatctg tactttcaga gcgataacga
     5461 tggatccgaa atcggtactg gctttccatt cgacccccat tatgtggaag tcctgggcga
     5521 gcgcatgcac tacgtcgatg ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg
     5581 taacccgacc tcctcctacg tgtggcgcaa catcatcccg catgttgcac cgacccatcg
     5641 ctgcattgct ccagacctga tcggtatggg caaatccgac aaaccagacc tgggttattt
     5701 cttcgacgac cacgtccgct tcatggatgc cttcatcgaa gccctgggtc tggaagaggt
     5761 cgtcctggtc attcacgact ggggctccgc tctgggtttc cactgggcca agcgcaatcc
     5821 agagcgcgtc aaaggtattg catttatgga gttcatccgc cctatcccga cctgggacga
     5881 atggccagaa tttgcccgcg agaccttcca ggccttccgc accaccgacg tcggccgcaa
     5941 gctgatcatc gatcagaacg tttttatcga gggtacgctg ccgatgggtg tcgtccgccc
     6001 gctgactgaa gtcgagatgg accattaccg cgagccgttc ctgaatcctg ttgaccgcga
     6061 gccactgtgg cgcttcccaa acgagctgcc aatcgccggt gagccagcga acatcgtcgc
     6121 gctggtcgaa gaatacatgg actggctgca ccagtcccct gtcccgaagc tgctgttctg
     6181 gggcacccca ggcgttctga tcccaccggc cgaagccgct cgcctggcca aaagcctgcc
     6241 taactgcaag gctgtggaca tcggcccggg tctgaatctg ctgcaagaag acaacccgga
     6301 cctgatcggc agcgagatcg cgcgctggct gtctactctg gagatttccg gttaaggccg
     6361 cactcctcag ctagaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     6421 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6481 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6541 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6601 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6661 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6721 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6781 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6841 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6901 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6961 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
     7021 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     7081 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     7141 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7201 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     7261 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     7321 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     7381 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     7441 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     7501 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7561 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7621 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7681 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     7741 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     7801 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     7861 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     7921 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     7981 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     8041 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     8101 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     8161 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     8221 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     8281 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     8341 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     8401 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8461 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8521 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8581 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8641 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8701 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8761 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     8821 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     8881 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     8941 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     9001 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     9061 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     9121 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     9181 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     9241 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     9301 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     9361 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     9421 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9481 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9541 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9601 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9661 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9721 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9781 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9841 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9901 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9961 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    10021 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10081 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10141 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10201 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10261 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10321 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10381 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10441 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10501 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10561 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10621 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10681 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10741 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10801 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10861 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10921 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10981 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    11041 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    11101 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    11161 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    11221 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    11281 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    11341 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    11401 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11461 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11521 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11581 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11641 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11701 cctgac
//
