LOCUS       Exported               11147 bp DNA     circular SYN 10-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Jason Vevea
  TITLE     Direct Submission
  JOURNAL   Exported Dec 1, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     primer_bind     2582..2620
                     /label=Fragment.FOR
     misc_feature    2612..3900
                     /label=CamKIIa Promoter
                     /note="Sequence from Nick"
     CDS             3922..3984
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     misc_feature    3991..4737
                     /label=YbeJ5-253
                     /note="YbeJ5-253"
     misc_feature    4192..4194
                     /label=S72A mutation
     CDS             4738..5013
                     /codon_start=1
                     /label=sf cpGFP 147-238
                     /note="cpGFP 147-238"
                     /translation="SHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDG
                     PVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    5014..5031
                     /label=Linker
                     /note="Linker"
     CDS             5032..5469
                     /codon_start=1
                     /label=sf cpGFP 1-146
                     /note="cpGFP 1-146"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFN"
     misc_feature    5470..5526
                     /label=YbeJ 254-272
                     /note="YbeJ 254-272"
     misc_feature    5527..5547
                     /label=YbeJ %C6272-280
                     /note="YbeJ %C6272-280"
     CDS             5560..5589
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5593..5715
                     /codon_start=1
                     /label=PDGFR TMD 513-554
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILI"
     CDS             5716..5751
                     /codon_start=1
                     /product="Tribasic golgi export signal KRRERRR
                     Parmar, H.B., Barry, C., Kai, F. and Duncan, R., 2014. 
                     Golgi complex?plasma membrane trafficking directed by an 
                     autonomous, tribasic Golgi export signal. Molecular biology
                     of the cell, 25(6), pp.866-878."
                     /label=Golgi Export signal
                     /translation="KYLQKRRERRRQ"
     CDS             5752..5793
                     /codon_start=1
                     /product="(FxYENEV) in the cytoplasmic C-terminus from
                     mammalian Kir2.1 channels
                     Stockklausner, C., Ludwig, J., Ruppersberg, J.P. and 
                     Klocker, N., 2001. A sequence motif responsible for ER 
                     export and surface expression of Kir2. 0 inward rectifier 
                     K+ channels. FEBS letters, 493(2-3), pp.129-133."
                     /label=ER export signal
                     /translation="ENANSFCYENEVAL"
     misc_feature    5839..6427
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(6310..6321)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6430..6446)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6952..7132
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7164..7388
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7434..7862
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7876..8205
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8056..8191
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8253..8300
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8319..8693
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8823..8944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8993..9009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9017..9033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9041..9071)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9086..9107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9395..9983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10154..11014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11015..11119)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttagagg ggtcggcaat tcattatggc cttaggtcac ttcatctcca
     2641 tggggttctt cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg
     2701 acctgcccag ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct
     2761 taaattccat gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg
     2821 cagatgacca atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc
     2881 ccagccctgg gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac
     2941 cttgacggga agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg
     3001 tccctctggg aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc
     3061 tcagtggtga cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc
     3121 tgtttggtac ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca
     3181 gggagagctg gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca
     3241 gaggaagcaa agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag
     3301 cttcccctgt aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct
     3361 tcagaggaga tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga
     3421 atgtggcacc cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc
     3481 cgtctgttaa aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt
     3541 gtggactaag tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa
     3601 cagggtccac ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg
     3661 caaagaggag caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg
     3721 caacgggaac agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct
     3781 cgcgaggctg tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc
     3841 ggttctccgt ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc
     3901 cggtaccgcg ggcccgccac catggagaca gacacactcc tgctatgggt actgctgctc
     3961 tgggttccag gttccactgg tgacagatct gccgcaggca gcacgctgga caaaatcgcc
     4021 aaaaacggtg tgattgtcgt cggtcaccgt gaatcttcag tgcctttctc ttattacgac
     4081 aatcagcaaa aagtggtggg ttactcgcag gattactcca acgccattgt tgaagcagtg
     4141 aaaaagaaac tcaacaaacc ggacttgcag gtaaaactga ttccgattac cgcacaaaac
     4201 cgtattccac tgctgcaaaa cggcactttc gattttgaat gtggttctac caccaacaac
     4261 gtcgaacgcc aaaaacaggc ggctttctct gacactattt tcgtggtcgg tacgcgcctg
     4321 ttgaccaaaa agggtggcga tatcaaagat tttgccaacc tgaaagacaa agccgtagtc
     4381 gtcacttccg gcactacctc tgaagttttg ctcaacaaac tgaatgaaga gcaaaaaatg
     4441 aatatgcgca tcatcagcgc caaagatcac ggtgactctt tccgcaccct ggaaagcggt
     4501 cgtgccgttg cctttatgat ggatgacgtg ctgctggccg gtgaacgtgc gaaagcgaag
     4561 aaaccagaca actgggaaat cgtcggcaag ccgcagtctc aggaggccta cggttgtatg
     4621 ttgcgtaaag atgatccgca gttcaaaaag ctgatggatg acaccatcgc tcaggtgcag
     4681 acctccggtg aagcggaaaa atggtttgat aagtggttca aaaatccaat tctggtgagc
     4741 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggcgaa cttcaagatc
     4801 cgccacaacg tggaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     4861 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgtgctg
     4921 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4981 gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag catgagcaag
     5041 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     5101 ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg caagctgacc
     5161 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     5221 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     5281 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcagctt caaggacgac
     5341 ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     5401 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5461 aactttaaca accccctgaa catgaatttc gaactgtcag acgaaatgaa agcactgttc
     5521 aaagaaccga atgacaaggc actgaaactg caggtcgacg aacaaaaact catctcagaa
     5581 gaggatctga atgctgtggg ccaggacacg caggaggtca tcgtggtgcc acactccttg
     5641 ccctttaagg tggtggtgat ctcagccatc ctggccctgg tggtgctcac catcatctcc
     5701 cttatcatcc tcatcaagta cctgcagaaa agaagggagc ggagacgcca ggagaacgca
     5761 aacagctttt gctatgaaaa tgaagttgcc ctctaaggcc gcactcctca gctagaattc
     5821 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5881 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5941 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6001 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6061 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6121 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6181 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6241 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6301 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6361 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6421 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
     6481 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     6541 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     6601 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     6661 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     6721 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     6781 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     6841 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     6901 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     6961 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7021 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7081 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     7141 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     7201 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     7261 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     7321 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     7381 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     7441 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7501 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     7561 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     7621 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     7681 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     7741 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     7801 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     7861 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7921 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7981 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     8041 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8101 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     8161 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     8221 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     8281 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     8341 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     8401 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     8461 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     8521 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     8581 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     8641 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     8701 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     8761 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     8821 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8881 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8941 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     9001 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     9061 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     9121 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     9181 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9241 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9301 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9361 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9421 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9481 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9541 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9601 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9661 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9721 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9781 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9841 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9901 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9961 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    10021 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    10081 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    10141 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    10201 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10261 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10321 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10381 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10441 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10501 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10561 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10621 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10681 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10741 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10801 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10861 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10921 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10981 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    11041 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    11101 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
