LOCUS       B1_pPOTv7_blast_        5527 bp    DNA        circular     08-MAR-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Addgene hts: updated seq at mChewrry ctd
COMMENT     
COMMENT     C terminal tag:
COMMENT     For: ggttctggtagtggttcc
COMMENT     Rev: ccaatttgagagacctgtgc
COMMENT     
COMMENT     N terminal tag
COMMENT     For: gtataatgcagacctgctgc
COMMENT     Rev: actacccgatcctgatcc
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             join(171..442,444..481,483..560)
                     /locus_tag="BLAST"
                     /label="BLAST"
                     /ApEinfo_label="BLAST"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(2067..2338,2340..2377,2379..2456)
                     /locus_tag="BLAST(1)"
                     /label="BLAST(1)"
                     /ApEinfo_label="BLAST(1)"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1671..1700
                     /locus_tag="GS linker"
                     /label="GS linker"
                     /ApEinfo_label="GS linker"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     5'UTR           36..164
                     /locus_tag="Actin 5'"
                     /label="Actin 5'"
                     /ApEinfo_label="Actin 5'"
                     /ApEinfo_fwdcolor="#ffd327"
                     /ApEinfo_revcolor="#ffd327"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             960..1664
                     /locus_tag="mCherry - codon optimised"
                     /label="mCherry - codon optimised"
                     /ApEinfo_label="mCherry - codon optimised"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     5'UTR           join(725..744,746..913)
                     /locus_tag="Aldolase 5'"
                     /label="Aldolase 5'"
                     /ApEinfo_label="Aldolase 5'"
                     /ApEinfo_fwdcolor="#ffd327"
                     /ApEinfo_revcolor="#ffd327"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     16..35
                     /locus_tag="#161"
                     /label="#161"
                     /ApEinfo_label="#161"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2615..2634)
                     /locus_tag="#162"
                     /label="#162"
                     /ApEinfo_label="#162"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           570..718
                     /locus_tag="Ald3'UTR (short)"
                     /label="Ald3'UTR (short)"
                     /ApEinfo_label="Ald3'UTR (short)"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           join(1710..1721,1723..1905,1907..2060)
                     /locus_tag="PFR2 IGS"
                     /label="PFR2 IGS"
                     /ApEinfo_label="PFR2 IGS"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           2466..2614
                     /locus_tag="Ald3'UTR (short)(1)"
                     /label="Ald3'UTR (short)(1)"
                     /ApEinfo_label="Ald3'UTR (short)(1)"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     5487..5504
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2684..2704)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     join(5503..5527,1..3)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2653..2678)
                     /locus_tag="SP6"
                     /label="SP6"
                     /ApEinfo_label="SP6"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(3072..3754)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(5024..5330)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(5348..5416)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    complement(2710..2732)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#ff4809"
                     /ApEinfo_revcolor="#ff4809"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(3852..4511)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    924..953
                     /locus_tag="GS linker(1)"
                     /label="GS linker(1)"
                     /ApEinfo_label="GS linker(1)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 GGGCGAATTG GGCCCgtata atgcagacct gctgcAAGCT AGGGGCACAG CAAGGTCTTC
       61 TGAAATTCAT GTTTTTTTTT TTTTTACTCT GCATTGCAGT CTCCGCTCTT ATTTAGTTTT
      121 GCTTTACGTA AGGTCTCGTT GCTGCCATAA AATAAGCTCT AGTGgaattc ATGCCTTTGT
      181 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT
      241 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG
      301 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG
      361 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA
      421 TCTTGAGCCC CTGCGGACGG TGcCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG
      481 CcATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT
      541 CTGGTTATGT GTGGGAGGGC TAAccatggT GCCCATTTAG TTGGCTTTTC CCTTGTCTCG
      601 TGTCTTTTCC GTGGAAAGGT TCCCGGAGTA ATCTGATGGC ACAGCAGGGA GGTGCGCCTG
      661 CAGGTTGGTT AGGAAGGGGG GATGATGTAA AAGAAGAAAA TGGGGGGATT CGAGCCCCac
      721 gcgtTGCTCA AGCTGTGTAG CGCAgGCGTT TCCTTACATA TTTCTCTAAC AGGCACGGAA
      781 GCCTAACAAA TACACTTGGC TTATTTTTTT GCCCCCTCAT GTCTTGTACA AATATTTGCG
      841 ATAGCTTAGC TATCAGCCAC ATTAATCAAA CAAGTATACC AACAAGCCCG AAAACAGAAA
      901 CTCAACTGCA ACGggtaccT ATGGGTTCTG GTAGTGGTTC CGGTTCCGGT TCTaagcttG
      961 TTAGTAAAGG AGAAGAAAAT AACATGGCAA TCATTAAGGA GTTCATGAGA TTCAAAGTTC
     1021 ACATGGAAGG TTCTGTAAAT GGACATGAAT TTGAAATAGA AGGTGAAGGA GAAGGAAGGC
     1081 CTTATGAAGG AACCCAAACC GCGAAGCTAA AAGTTACTAA GGGTGGCCCA TTACCATTTG
     1141 CATGGGATAT CCTTAGCCCT CAATTCATGT ATGGGTCAAA GGCTTATGTC AAGCACCCCG
     1201 CCGACATTCC AGACTATCTA AAGTTATCTT TTCCCGAAGG GTTTAAGTGG GAGCGTGTGA
     1261 TGAACTTCGA AGACGGTGGC GTGGTAACAG TGACTCAGGA TTCGTCCCTG CAAGATGGTG
     1321 AATTTATCTA CAAAGTCAAA TTAAGAGGAA CTAACTTTCC ATCTGACGGC CCGGTTATGC
     1381 AAAAAAAGAC AATGGGCTGG GAGGCCTCCT CAGAACGAAT GTACCCTGAA GATGGTGCCT
     1441 TGAAGGGTGA GATTAAACAA AGATTGAAAT TGAAAGATGG TGGACATTAT GACGCTGAgG
     1501 TTAAAACGAC ATACAAAGCT AAGAAACCTG TCCAGCTCCC AGGTGCTTAC AATGTAAATA
     1561 TAAAACTTGA TATTACATCA CATAATGAAG ATTATACGAT AGTTGAACAA TACGAAAGGG
     1621 cTGAGGGgAG ACATAGTACT GGTGGCATGG aTGAACTATA CAAAggatcc GGATCAGGAT
     1681 CTGGATCAGG ATCGGGTAGT TAAgagctcC GCTGCGCTTA AATGTCTTTC ATTATAATCA
     1741 ATGTATAACC TTTATGTAGT ATTTCAATCT ATGCCGCTGT GTACGTGCAC TGCGGTGCCT
     1801 ATCCTTCGGC ATTAGAGAGT CACTGTTTGT GTAGATCGTA GCTGCATGTC TGACATCTGG
     1861 TTCATTAGTG CCGTTTTGAG TCTGCACTGT TGTGCAACTT GATATTCATC ACTGCACACC
     1921 AACCGTGTGT TTCCTCCTTT GGGTGCACCC TTTAATCCTT GTCTTCTCCT TTTTTGTCTC
     1981 TTTCCCCCTC GAAAAGGTGT CAAACTACTG CCGCATAAAC TACGGTTATC CCAAATTTAA
     2041 GAGAAAGCAA TAAAGCATCA gctagcATGC CTTTGTCTCA AGAAGAATCC ACCCTCATTG
     2101 AAAGAGCAAC GGCTACAATC AACAGCATCC CCATCTCTGA AGACTACAGC GTCGCCAGCG
     2161 CAGCTCTCTC TAGCGACGGC CGCATCTTCA CTGGTGTCAA TGTATATCAT TTTACTGGGG
     2221 GACCTTGTGC AGAACTCGTG GTGCTGGGCA CTGCTGCTGC TGCGGCAGCT GGCAACCTGA
     2281 CTTGTATCGT CGCGATCGGA AATGAGAACA GGGGCATCTT GAGCCCCTGC GGACGGTGcC
     2341 GACAGGTGCT TCTCGATCTG CATCCTGGGA TCAAAGCcAT AGTGAAGGAC AGTGATGGAC
     2401 AGCCGACGGC AGTTGGGATT CGTGAATTGC TGCCCTCTGG TTATGTGTGG GAGGGCTAAc
     2461 tcgagTGCCC ATTTAGTTGG CTTTTCCCTT GTCTCGTGTC TTTTCCGTGG AAAGGTTCCC
     2521 GGAGTAATCT GATGGCACAG CAGGGAGGTG CGCCTGCAGG TTGGTTAGGA AGGGGGGATG
     2581 ATGTAAAAGA AGAAAATGGG GGGATTCGAG CCCCGCACAG GTCTCTCAAA TTGGATGCAT
     2641 AGCTTGAGTA TTCTATAGTG TCACCTAAAT AGCTTGGCGT AATCATGGTC ATAGCTGTTT
     2701 CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG
     2761 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG
     2821 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG
     2881 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC
     2941 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
     3001 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
     3061 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
     3121 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
     3181 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
     3241 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
     3301 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
     3361 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
     3421 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
     3481 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT
     3541 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
     3601 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
     3661 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     3721 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     3781 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     3841 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     3901 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     3961 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA
     4021 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     4081 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     4141 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     4201 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     4261 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     4321 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     4381 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     4441 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     4501 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     4561 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     4621 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     4681 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     4741 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     4801 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ATGCGGTGTG AAATACCGCA
     4861 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GAAATTGTAA GCGTTAATAT TTTGTTAAAA
     4921 TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA
     4981 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC
     5041 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG
     5101 GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT
     5161 AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG
     5221 GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA
     5281 AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG
     5341 GGCGCGTCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
     5401 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
     5461 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTG TAATACGACT
     5521 CACTATA     
//
