LOCUS       Bx_pPOTv7_blast_        5534 bp    DNA        circular     30-OCT-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     C terminal tag:
COMMENT     For: ggttctggtagtggttcc
COMMENT     Rev: ccaatttgagagacctgtgc
COMMENT     
COMMENT     N terminal tag
COMMENT     For: gtataatgcagacctgctgc
COMMENT     Rev: actacccgatcctgatcc
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             join(171..442,444..481,483..560)
                     /locus_tag="BLAST"
                     /label="BLAST"
                     /ApEinfo_label="BLAST"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(2074..2345,2347..2384,2386..2463)
                     /locus_tag="BLAST(1)"
                     /label="BLAST(1)"
                     /ApEinfo_label="BLAST(1)"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1677..1706
                     /locus_tag="GS linker"
                     /label="GS linker"
                     /ApEinfo_label="GS linker"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     5'UTR           36..164
                     /locus_tag="Actin 5'"
                     /label="Actin 5'"
                     /ApEinfo_label="Actin 5'"
                     /ApEinfo_fwdcolor="#ffd327"
                     /ApEinfo_revcolor="#ffd327"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     5'UTR           join(725..744,746..913)
                     /locus_tag="Aldolase 5'"
                     /label="Aldolase 5'"
                     /ApEinfo_label="Aldolase 5'"
                     /ApEinfo_fwdcolor="#ffd327"
                     /ApEinfo_revcolor="#ffd327"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     16..35
                     /locus_tag="#161"
                     /label="#161"
                     /ApEinfo_label="#161"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2622..2641)
                     /locus_tag="#162"
                     /label="#162"
                     /ApEinfo_label="#162"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           570..718
                     /locus_tag="Ald3'UTR (short)"
                     /label="Ald3'UTR (short)"
                     /ApEinfo_label="Ald3'UTR (short)"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           join(1716..1727,1729..1911,1913..2066)
                     /locus_tag="PFR2 IGS"
                     /label="PFR2 IGS"
                     /ApEinfo_label="PFR2 IGS"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           2473..2621
                     /locus_tag="Ald3'UTR (short)(1)"
                     /label="Ald3'UTR (short)(1)"
                     /ApEinfo_label="Ald3'UTR (short)(1)"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     5494..5511
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2691..2711)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     join(5510..5534,1..3)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2660..2685)
                     /locus_tag="SP6"
                     /label="SP6"
                     /ApEinfo_label="SP6"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(3079..3761)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(5031..5337)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(5355..5423)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    complement(2717..2739)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#ff4809"
                     /ApEinfo_revcolor="#ff4809"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(3859..4518)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    924..953
                     /locus_tag="GS linker(1)"
                     /label="GS linker(1)"
                     /ApEinfo_label="GS linker(1)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             960..1670
                     /locus_tag="mRuby3"
                     /label="mRuby3"
                     /ApEinfo_label="mRuby3"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 GGGCGAATTG GGCCCgtata atgcagacct gctgcAAGCT AGGGGCACAG CAAGGTCTTC
       61 TGAAATTCAT GTTTTTTTTT TTTTTACTCT GCATTGCAGT CTCCGCTCTT ATTTAGTTTT
      121 GCTTTACGTA AGGTCTCGTT GCTGCCATAA AATAAGCTCT AGTGgaattc ATGCCTTTGT
      181 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT
      241 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG
      301 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG
      361 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA
      421 TCTTGAGCCC CTGCGGACGG TGcCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG
      481 CcATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT
      541 CTGGTTATGT GTGGGAGGGC TAAccatggT GCCCATTTAG TTGGCTTTTC CCTTGTCTCG
      601 TGTCTTTTCC GTGGAAAGGT TCCCGGAGTA ATCTGATGGC ACAGCAGGGA GGTGCGCCTG
      661 CAGGTTGGTT AGGAAGGGGG GATGATGTAA AAGAAGAAAA TGGGGGGATT CGAGCCCCac
      721 gcgtTGCTCA AGCTGTGTAG CGCAgGCGTT TCCTTACATA TTTCTCTAAC AGGCACGGAA
      781 GCCTAACAAA TACACTTGGC TTATTTTTTT GCCCCCTCAT GTCTTGTACA AATATTTGCG
      841 ATAGCTTAGC TATCAGCCAC ATTAATCAAA CAAGTATACC AACAAGCCCG AAAACAGAAA
      901 CTCAACTGCA ACGggtaccT ATGGGTTCTG GTAGTGGTTC CGGTTCCGGT TCTaagcttA
      961 TGGTTTCCAA GGGGGAAGAA CTGATAAAGG AAAATATGCG CATGAAAGTA GTGATGGAGG
     1021 GGAGTGTAAA TGGTCATCAA TTTAAATGCA CTGGTGAGGG TGAAGGCCGA CCCTACGAAG
     1081 GGGTCCAGAC GATGCGGATC AAGGTAATCG AGGGAGGACC GTTGCCTTTC GCATTTGATA
     1141 TTCTTGCCAC CTCTTTCATG TATGGATCAC GAACCTTCAT TAAGTACCCC GCAGACATCC
     1201 CAGATTTCTT TAAACAGTCT TTCCCCGAGG GGTTCACTTG GGAGCGAGTG ACGCGATATG
     1261 AGGACGGGGG TGTTGTAACT GTGACGCAGG ACACCAGCTT GGAAGACGGT GAGTTGGTTT
     1321 ATAATGTCAA GGTACGTGGT GTAAATTTTC CCTCTAACGG TCCTGTTATG CAAAAGAAAA
     1381 CTAAGGGATG GGAGCCCAAC ACGGAGATGA TGTATCCCGC GGACGGTGGT CTGAGGGGAT
     1441 ACACAGATAT TGCTCTTAAA GTTGACGGCG GTGGACACTT GCATTGCAAC TTTGTCACTA
     1501 CTTATCGTAG TAAAAAGACT GTAGGAAACA TTAAAATGCC TGGAGTTCAC GCTGTGGATC
     1561 ACCGATTGGA ACGGATTGAG GAGTCCGATA ATGAAACCTA CGTAGTACAA CGTGAAGTTG
     1621 CTGTGGCAAA GTATTCAAAC CTGGGGGGAG GAATGGATGA GCTTTATAAG ggatccGGAT
     1681 CAGGATCTGG ATCAGGATCG GGTAGTTAAg agctcCGCTG CGCTTAAATG TCTTTCATTA
     1741 TAATCAATGT ATAACCTTTA TGTAGTATTT CAATCTATGC CGCTGTGTAC GTGCACTGCG
     1801 GTGCCTATCC TTCGGCATTA GAGAGTCACT GTTTGTGTAG ATCGTAGCTG CATGTCTGAC
     1861 ATCTGGTTCA TTAGTGCCGT TTTGAGTCTG CACTGTTGTG CAACTTGATA TTCATCACTG
     1921 CACACCAACC GTGTGTTTCC TCCTTTGGGT GCACCCTTTA ATCCTTGTCT TCTCCTTTTT
     1981 TGTCTCTTTC CCCCTCGAAA AGGTGTCAAA CTACTGCCGC ATAAACTACG GTTATCCCAA
     2041 ATTTAAGAGA AAGCAATAAA GCATCAgcta gcnATGCCTT TGTCTCAAGA AGAATCCACC
     2101 CTCATTGAAA GAGCAACGGC TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC
     2161 GCCAGCGCAG CTCTCTCTAG CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT
     2221 ACTGGGGGAC CTTGTGCAGA ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC
     2281 AACCTGACTT GTATCGTCGC GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA
     2341 CGGTGcCGAC AGGTGCTTCT CGATCTGCAT CCTGGGATCA AAGCcATAGT GAAGGACAGT
     2401 GATGGACAGC CGACGGCAGT TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG
     2461 GGCTAActcg agTGCCCATT TAGTTGGCTT TTCCCTTGTC TCGTGTCTTT TCCGTGGAAA
     2521 GGTTCCCGGA GTAATCTGAT GGCACAGCAG GGAGGTGCGC CTGCAGGTTG GTTAGGAAGG
     2581 GGGGATGATG TAAAAGAAGA AAATGGGGGG ATTCGAGCCC CGCACAGGTC TCTCAAATTG
     2641 GATGCATAGC TTGAGTATTC TATAGTGTCA CCTAAATAGC TTGGCGTAAT CATGGTCATA
     2701 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG
     2761 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG
     2821 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA
     2881 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC
     2941 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG
     3001 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA
     3061 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
     3121 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     3181 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     3241 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     3301 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     3361 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     3421 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     3481 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     3541 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     3601 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     3661 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     3721 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     3781 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     3841 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     3901 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     3961 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
     4021 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     4081 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     4141 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
     4201 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     4261 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     4321 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     4381 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     4441 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
     4501 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     4561 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     4621 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     4681 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     4741 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     4801 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGATG CGGTGTGAAA
     4861 TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGAA ATTGTAAGCG TTAATATTTT
     4921 GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT
     4981 CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT
     5041 TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT
     5101 CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG
     5161 GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG
     5221 AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC
     5281 GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC
     5341 GCTACAGGGC GCGTCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG
     5401 CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT
     5461 TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGTAA
     5521 TACGACTCAC TATA    
//
