LOCUS       Bx_pPOTv7_blast_        5531 bp    DNA        circular     30-OCT-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     C terminal tag:
COMMENT     For: ggttctggtagtggttcc
COMMENT     Rev: ccaatttgagagacctgtgc
COMMENT     
COMMENT     N terminal tag
COMMENT     For: gtataatgcagacctgctgc
COMMENT     Rev: actacccgatcctgatcc
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             join(171..442,444..481,483..560)
                     /locus_tag="BLAST"
                     /label="BLAST"
                     /ApEinfo_label="BLAST"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(2071..2342,2344..2381,2383..2460)
                     /locus_tag="BLAST(1)"
                     /label="BLAST(1)"
                     /ApEinfo_label="BLAST(1)"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1674..1703
                     /locus_tag="GS linker"
                     /label="GS linker"
                     /ApEinfo_label="GS linker"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     exon            960..1667
                     /locus_tag="LSSmOrange"
                     /label="LSSmOrange"
                     /ApEinfo_label="LSSmOrange"
                     /ApEinfo_fwdcolor="#c83a90"
                     /ApEinfo_revcolor="#c83a90"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             960..1667
                     /locus_tag="LSSmOrange(1)"
                     /label="LSSmOrange(1)"
                     /ApEinfo_label="LSSmOrange(1)"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     5'UTR           36..164
                     /locus_tag="Actin 5'"
                     /label="Actin 5'"
                     /ApEinfo_label="Actin 5'"
                     /ApEinfo_fwdcolor="#ffd327"
                     /ApEinfo_revcolor="#ffd327"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     5'UTR           join(725..744,746..913)
                     /locus_tag="Aldolase 5'"
                     /label="Aldolase 5'"
                     /ApEinfo_label="Aldolase 5'"
                     /ApEinfo_fwdcolor="#ffd327"
                     /ApEinfo_revcolor="#ffd327"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     16..35
                     /locus_tag="#161"
                     /label="#161"
                     /ApEinfo_label="#161"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2619..2638)
                     /locus_tag="#162"
                     /label="#162"
                     /ApEinfo_label="#162"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           570..718
                     /locus_tag="Ald3'UTR (short)"
                     /label="Ald3'UTR (short)"
                     /ApEinfo_label="Ald3'UTR (short)"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           join(1713..1724,1726..1908,1910..2063)
                     /locus_tag="PFR2 IGS"
                     /label="PFR2 IGS"
                     /ApEinfo_label="PFR2 IGS"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           2470..2618
                     /locus_tag="Ald3'UTR (short)(1)"
                     /label="Ald3'UTR (short)(1)"
                     /ApEinfo_label="Ald3'UTR (short)(1)"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     5491..5508
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2688..2708)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     join(5507..5531,1..3)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2657..2682)
                     /locus_tag="SP6"
                     /label="SP6"
                     /ApEinfo_label="SP6"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(3076..3758)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(5028..5334)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(5352..5420)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    complement(2714..2736)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#ff4809"
                     /ApEinfo_revcolor="#ff4809"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(3856..4515)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    924..953
                     /locus_tag="GS linker(1)"
                     /label="GS linker(1)"
                     /ApEinfo_label="GS linker(1)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 GGGCGAATTG GGCCCgtata atgcagacct gctgcAAGCT AGGGGCACAG CAAGGTCTTC
       61 TGAAATTCAT GTTTTTTTTT TTTTTACTCT GCATTGCAGT CTCCGCTCTT ATTTAGTTTT
      121 GCTTTACGTA AGGTCTCGTT GCTGCCATAA AATAAGCTCT AGTGgaattc ATGCCTTTGT
      181 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT
      241 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG
      301 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG
      361 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA
      421 TCTTGAGCCC CTGCGGACGG TGcCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG
      481 CcATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT
      541 CTGGTTATGT GTGGGAGGGC TAAccatggT GCCCATTTAG TTGGCTTTTC CCTTGTCTCG
      601 TGTCTTTTCC GTGGAAAGGT TCCCGGAGTA ATCTGATGGC ACAGCAGGGA GGTGCGCCTG
      661 CAGGTTGGTT AGGAAGGGGG GATGATGTAA AAGAAGAAAA TGGGGGGATT CGAGCCCCac
      721 gcgtTGCTCA AGCTGTGTAG CGCAgGCGTT TCCTTACATA TTTCTCTAAC AGGCACGGAA
      781 GCCTAACAAA TACACTTGGC TTATTTTTTT GCCCCCTCAT GTCTTGTACA AATATTTGCG
      841 ATAGCTTAGC TATCAGCCAC ATTAATCAAA CAAGTATACC AACAAGCCCG AAAACAGAAA
      901 CTCAACTGCA ACGggtaccT ATGGGTTCTG GTAGTGGTTC CGGTTCCGGT TCTaagcttA
      961 TGGTTTCTAA GGGTGAAGAA AATAACATGG CCATTATTAA AGAGTTTATG AGGTTCAAAG
     1021 TGCGCATGGA AGGATCGGTA AATGGTCACG AGTTTGAGAT TGAAGGAGAG GGCGAGGGTC
     1081 GGCCTTATGA AGGCTTCCAA ACAGTCAAGC TCAAGGTGAC AAAGGGCGGG CCTCTCCCGT
     1141 TCGCATGGGA CATACTCAGT CCCCAATTCA CCTATGGTTC TAAGGCGTAT GTCAAGCATC
     1201 CAGCTGACAT ACCGGACTAC CTCAAGTTGT CTTTCCCCGA GGGTTTTAAG TGGGAACGAG
     1261 TAATGAACTT TGAGGATGGT GGAGTCGTTA CTGTCACTCA GGATTCCAGT CTGCAAGATG
     1321 GGGAGTTCAT CTACAAGGTC AAGTTGCGTG GCACGAACTT TCCCAGTGAC GGCCCCGTGA
     1381 TGCAAAAGAA AACGATGGGG ATGGAAGCAT CATCTGAAAG GATGTACCCA GAAGATGGGG
     1441 CACTTAAAGG GGAAGATAAA CTGCGGCTTA AATTGAAAGA TGGCGGTCAC TACACATCAG
     1501 AGGTTAAGAC AACCTACAAG GCTAAAAAGC CAGTGCAGCT CCCAGGAGCG TACATAGTGG
     1561 ACATCAAACT GGACATAACC TCTCACAACG AGGACTACAC CATTGTCGAA CAATACGAAC
     1621 GAGCTGAAGG TCGTCATTCG ACCGGAGGTA TGGACGAACT TTACAAGgga tccGGATCAG
     1681 GATCTGGATC AGGATCGGGT AGTTAAgagc tcCGCTGCGC TTAAATGTCT TTCATTATAA
     1741 TCAATGTATA ACCTTTATGT AGTATTTCAA TCTATGCCGC TGTGTACGTG CACTGCGGTG
     1801 CCTATCCTTC GGCATTAGAG AGTCACTGTT TGTGTAGATC GTAGCTGCAT GTCTGACATC
     1861 TGGTTCATTA GTGCCGTTTT GAGTCTGCAC TGTTGTGCAA CTTGATATTC ATCACTGCAC
     1921 ACCAACCGTG TGTTTCCTCC TTTGGGTGCA CCCTTTAATC CTTGTCTTCT CCTTTTTTGT
     1981 CTCTTTCCCC CTCGAAAAGG TGTCAAACTA CTGCCGCATA AACTACGGTT ATCCCAAATT
     2041 TAAGAGAAAG CAATAAAGCA TCAgctagcn ATGCCTTTGT CTCAAGAAGA ATCCACCCTC
     2101 ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT CTGAAGACTA CAGCGTCGCC
     2161 AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG TCAATGTATA TCATTTTACT
     2221 GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG CTGCTGCGGC AGCTGGCAAC
     2281 CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA TCTTGAGCCC CTGCGGACGG
     2341 TGcCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG CcATAGTGAA GGACAGTGAT
     2401 GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT CTGGTTATGT GTGGGAGGGC
     2461 TAActcgagT GCCCATTTAG TTGGCTTTTC CCTTGTCTCG TGTCTTTTCC GTGGAAAGGT
     2521 TCCCGGAGTA ATCTGATGGC ACAGCAGGGA GGTGCGCCTG CAGGTTGGTT AGGAAGGGGG
     2581 GATGATGTAA AAGAAGAAAA TGGGGGGATT CGAGCCCCGC ACAGGTCTCT CAAATTGGAT
     2641 GCATAGCTTG AGTATTCTAT AGTGTCACCT AAATAGCTTG GCGTAATCAT GGTCATAGCT
     2701 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT
     2761 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC
     2821 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
     2881 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
     2941 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     3001 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     3061 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
     3121 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     3181 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     3241 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     3301 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     3361 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     3421 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     3481 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     3541 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     3601 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     3661 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     3721 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     3781 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     3841 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     3901 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     3961 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT
     4021 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     4081 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     4141 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     4201 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     4261 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     4321 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     4381 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     4441 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     4501 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     4561 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
     4621 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
     4681 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
     4741 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
     4801 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGATGCGG TGTGAAATAC
     4861 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGAAATT GTAAGCGTTA ATATTTTGTT
     4921 AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG
     4981 CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG
     5041 GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA
     5101 TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG
     5161 CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA
     5221 GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT
     5281 GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT
     5341 ACAGGGCGCG TCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
     5401 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG
     5461 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTGTAATAC
     5521 GACTCACTAT A    
//
