LOCUS       C1_pPOTv6_puro_p        6145 bp ds-DNA     circular     23-DEC-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     C terminal tag:
COMMENT     For: ggttctggtagtggttcc
COMMENT     Rev: ccaatttgagagacctgtgc
COMMENT     
COMMENT     N terminal tag
COMMENT     For: gtataatgcagacctgctgc
COMMENT     Rev: actacccgatcctgatcc
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     5'UTR           36..164
                     /locus_tag="Actin 5'"
     5'UTR           join(932..951,953..1120)
                     /locus_tag="Aldolase 5'"
     misc_feature    1197..1226
                     /locus_tag="Ty2"
     CDS             1269..1973
                     /locus_tag="mNG"
     primer_bind     16..35
                     /locus_tag="#161"
     misc_feature    1233..1262
                     /locus_tag="Ty3"
     primer_bind     complement(3233..3252)
                     /locus_tag="#162"
     3'UTR           777..925
                     /locus_tag="Ald3'UTR (short)"
     3'UTR           3084..3232
                     /locus_tag="Ald3'UTR (short)(1)"
     primer_bind     6105..6122
                     /locus_tag="M13-fwd"
     misc_feature    2016..2045
                     /locus_tag="Ty9"
     primer_bind     complement(3302..3322)
                     /locus_tag="M13-rev"
     primer_bind     join(6121..6145,1..3)
                     /locus_tag="T7"
     misc_feature    2052..2081
                     /locus_tag="Ty10"
     primer_bind     complement(3271..3296)
                     /locus_tag="SP6"
     3'UTR           join(2121..2132,2134..2316,2318..2471)
                     /locus_tag="PFR2 IGS"
     rep_origin      complement(3690..4372)
                     /locus_tag="ColE1 origin"
     rep_origin      complement(5642..5948)
                     /locus_tag="F1 ori"
     CDS             complement(5966..6034)
                     /locus_tag="LacZ alpha"
     misc_binding    complement(3328..3350)
                     /locus_tag="LacO"
     CDS             complement(4470..5129)
                     /locus_tag="AmpR"
     CDS             171..770
                     /locus_tag="puromycin ORF"
     CDS             2478..3077
                     /locus_tag="puromycin ORF(1)"
     misc_feature    1161..1190
                     /locus_tag="Ty1"
     misc_feature    2082..2111
                     /locus_tag="GS linker"
     misc_feature    1131..1160
                     /locus_tag="GS linker(1)"
     misc_feature    1980..2009
                     /locus_tag="Ty8"
ORIGIN
        1 GGGCGAATTG GGCCCgtata atgcagacct gctgcAAGCT AGGGGCACAG CAAGGTCTTC
       61 TGAAATTCAT GTTTTTTTTT TTTTTACTCT GCATTGCAGT CTCCGCTCTT ATTTAGTTTT
      121 GCTTTACGTA AGGTCTCGTT GCTGCCATAA AATAAGCTCT AGTGgaattc ATGACTGAAT
      181 ACAAGCCAAC GGTTCGTCTT GCTACGCGAG ACGATGTGCC GCGGGCCGTA CGGACGCTGG
      241 CTGCTGCGTT CGCTGATTAT CCCGCCACCC GACATACGGT TGACCCAGAT CGACACATTG
      301 AGCGAGTCAC AGAACTTCAA GAGTTATTCT TAACACGGGT GGGCTTGGAC ATTGGTAAGG
      361 TTTGGGTTGC GGACGATGGC GCGGCTGTTG CGGTGTGGAC GACGCCAGAG TCAGTCGAAG
      421 CGGGCGCAGT GTTTGCTGAG ATCGGTCCCC GAATGGCGGA GCTTAGCGGG TCACGCCTCG
      481 CTGCACAACA GCAGATGGAA GGTCTGTTGG CACCACATAG ACCTAAGGAG CCGGCTTGGT
      541 TCTTAGCGAC AGTTGGCGTC AGTCCGGACC ACCAAGGTAA GGGGCTGGGT TCAGCGGTTG
      601 TTCTTCCAGG AGTAGAGGCT GCGGAAAGGG CAGGTGTGCC GGCGTTCCTT GAAACGTCTG
      661 CACCACGTAA CCTTCCTTTT TACGAGAGAT TGGGGTTTAC AGTAACAGCA GATGTTGAAG
      721 TGCCTGAAGG GCCGAGGACA TGGTGCATGA CACGTAAGCC GGGAGCCTAA ccatggTGCC
      781 CATTTAGTTG GCTTTTCCCT TGTCTCGTGT CTTTTCCGTG GAAAGGTTCC CGGAGTAATC
      841 TGATGGCACA GCAGGGAGGT GCGCCTGCAG GTTGGTTAGG AAGGGGGGAT GATGTAAAAG
      901 AAGAAAATGG GGGGATTCGA GCCCCacgcg tTGCTCAAGC TGTGTAGCGC AgGCGTTTCC
      961 TTACATATTT CTCTAACAGG CACGGAAGCC TAACAAATAC ACTTGGCTTA TTTTTTTGCC
     1021 CCCTCATGTC TTGTACAAAT ATTTGCGATA GCTTAGCTAT CAGCCACATT AATCAAACAA
     1081 GTATACCAAC AAGCCCGAAA ACAGAAACTC AACTGCAACG ggtaccTATG GGTTCTGGTA
     1141 GTGGTTCCGG TTCCGGTTCT GAGGTACACA CAAACCAGGA CCCACTGGAC GGCTCCGAGG
     1201 TGCATACCAA TCAAGATCCG CTGGACGGCT CTGAAGTTCA CACCAATCAA GATCCCTTGG
     1261 ATaagcttGT CTCGAAAGGT GAGGAAGACA ATATGGCCAG CTTGCCAGCA ACGCACGAGC
     1321 TTCATATATT TGGGTCGATC AACGGTGTGG ATTTCGACAT GGTGGGTCAA GGCACAGGTA
     1381 ATCCTAATGA CGGGTATGAA GAACTCAATT TGAAGAGCAC TAAGGGAGAT CTCCAGTTTT
     1441 CCCCGTGGAT TCTTGTGCCG CATATTGGTT ATGGTTTTCA TCAGTACTTA CCATACCCCG
     1501 ATGGAATGTC GCCCTTCCAA GCAGCTATGG TGGACGGCTC GGGGTATCAA GTACATCGCA
     1561 CCATGCAATT TGAGGACGGA GCATCCCTCA CCGTAAACTA CCGCTATACG TATGAGGGGA
     1621 GCCACATAAA AGGTGAGGCT CAAGTTAAAG GCACAGGCTT CCCAGCTGAC GGCCCGGTCA
     1681 TGACCAATTC ATTAACCGCT GCTGATTGGT GCCGTTCTAA GAAGACCTAC CCTAACGACA
     1741 AGACGATCAT TAGTACTTTT AAGTGGAGTT ATACCACAGG AAACGGAAAG CGGTATCGAA
     1801 GCACTGCTCG AACAACCTAC ACATTTGCGA AGCCAATGGC CGCGAACTAC TTGAAGAACC
     1861 AGCCAATGTA TGTGTTTCGA AAAACGGAAC TGAAGCATTC GAAAACCGAG CTTAATTTTA
     1921 AAGAGTGGCA GAAGGCGTTT ACAGACGTGA TGGGGATGGA CGAATTGTAT AAGggatccG
     1981 AGGTACATAC AAATCAGGAC CCCCTCGATG GTAGTGAAGT CCATACAAAT CAGGACCCGC
     2041 TGGATGGCTC AGAGGTTCAT ACGAACCAAG ATCCTCTTGA TGGATCAGGA TCTGGATCAG
     2101 GATCGGGTAG TTAAgagctc CGCTGCGCTT AAATGTCTTT CATTATAATC AATGTATAAC
     2161 CTTTATGTAG TATTTCAATC TATGCCGCTG TGTACGTGCA CTGCGGTGCC TATCCTTCGG
     2221 CATTAGAGAG TCACTGTTTG TGTAGATCGT AGCTGCATGT CTGACATCTG GTTCATTAGT
     2281 GCCGTTTTGA GTCTGCACTG TTGTGCAACT TGATATTCAT CACTGCACAC CAACCGTGTG
     2341 TTTCCTCCTT TGGGTGCACC CTTTAATCCT TGTCTTCTCC TTTTTTGTCT CTTTCCCCCT
     2401 CGAAAAGGTG TCAAACTACT GCCGCATAAA CTACGGTTAT CCCAAATTTA AGAGAAAGCA
     2461 ATAAAGCATC AgctagcATG ACTGAATACA AGCCAACGGT TCGTCTTGCT ACGCGAGACG
     2521 ATGTGCCGCG GGCCGTACGG ACGCTGGCTG CTGCGTTCGC TGATTATCCC GCCACCCGAC
     2581 ATACGGTTGA CCCAGATCGA CACATTGAGC GAGTCACAGA ACTTCAAGAG TTATTCTTAA
     2641 CACGGGTGGG CTTGGACATT GGTAAGGTTT GGGTTGCGGA CGATGGCGCG GCTGTTGCGG
     2701 TGTGGACGAC GCCAGAGTCA GTCGAAGCGG GCGCAGTGTT TGCTGAGATC GGTCCCCGAA
     2761 TGGCGGAGCT TAGCGGGTCA CGCCTCGCTG CACAACAGCA GATGGAAGGT CTGTTGGCAC
     2821 CACATAGACC TAAGGAGCCG GCTTGGTTCT TAGCGACAGT TGGCGTCAGT CCGGACCACC
     2881 AAGGTAAGGG GCTGGGTTCA GCGGTTGTTC TTCCAGGAGT AGAGGCTGCG GAAAGGGCAG
     2941 GTGTGCCGGC GTTCCTTGAA ACGTCTGCAC CACGTAACCT TCCTTTTTAC GAGAGATTGG
     3001 GGTTTACAGT AACAGCAGAT GTTGAAGTGC CTGAAGGGCC GAGGACATGG TGCATGACAC
     3061 GTAAGCCGGG AGCCTAActc gagTGCCCAT TTAGTTGGCT TTTCCCTTGT CTCGTGTCTT
     3121 TTCCGTGGAA AGGTTCCCGG AGTAATCTGA TGGCACAGCA GGGAGGTGCG CCTGCAGGTT
     3181 GGTTAGGAAG GGGGGATGAT GTAAAAGAAG AAAATGGGGG GATTCGAGCC CCGCACAGGT
     3241 CTCTCAAATT GGATGCATAG CTTGAGTATT CTATAGTGTC ACCTAAATAG CTTGGCGTAA
     3301 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
     3361 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
     3421 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA
     3481 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG
     3541 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
     3601 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
     3661 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
     3721 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
     3781 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
     3841 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
     3901 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
     3961 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
     4021 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
     4081 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
     4141 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
     4201 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
     4261 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
     4321 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     4381 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     4441 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     4501 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     4561 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
     4621 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     4681 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     4741 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     4801 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     4861 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     4921 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     4981 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     5041 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     5101 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     5161 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     5221 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     5281 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     5341 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     5401 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAT
     5461 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGA AATTGTAAGC
     5521 GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA
     5581 TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT
     5641 GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG
     5701 CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT
     5761 TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA
     5821 GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG
     5881 GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG
     5941 CTTAATGCGC CGCTACAGGG CGCGTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG
     6001 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA
     6061 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA
     6121 GTGAATTGTA ATACGACTCA CTATA   
//
