LOCUS       C5_pPOTv6_myc_bl        5723 bp ds-DNA     circular     29-DEC-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     C terminal tag:
COMMENT     For: ggttctggtagtggttcc
COMMENT     Rev: ccaatttgagagacctgtgc
COMMENT     
COMMENT     N terminal tag
COMMENT     For: gtataatgcagacctgctgc
COMMENT     Rev: actacccgatcctgatcc
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             join(171..442,444..481,483..560)
                     /locus_tag="BLAST"
     CDS             join(2263..2534,2536..2573,2575..2652)
                     /locus_tag="BLAST(1)"
     5'UTR           36..164
                     /locus_tag="Actin 5'"
     5'UTR           join(725..744,746..913)
                     /locus_tag="Aldolase 5'"
     primer_bind     16..35
                     /locus_tag="#161"
     primer_bind     complement(2811..2830)
                     /locus_tag="#162"
     3'UTR           570..718
                     /locus_tag="Ald3'UTR (short)"
     3'UTR           join(1905..1916,1918..2100,2102..2255)
                     /locus_tag="PFR2 IGS"
     misc_feature    1836..1865
                     /locus_tag="Myc6"
     3'UTR           2662..2810
                     /locus_tag="Ald3'UTR (short)(1)"
     misc_feature    924..953
                     /locus_tag="GS linker"
     primer_bind     5683..5700
                     /locus_tag="M13-fwd"
     misc_feature    1866..1895
                     /locus_tag="GS linker(1)"
     primer_bind     complement(2880..2900)
                     /locus_tag="M13-rev"
     primer_bind     join(5699..5723,1..3)
                     /locus_tag="T7"
     primer_bind     complement(2849..2874)
                     /locus_tag="SP6"
     rep_origin      complement(3268..3950)
                     /locus_tag="ColE1 origin"
     rep_origin      complement(5220..5526)
                     /locus_tag="F1 ori"
     misc_feature    954..983
                     /locus_tag="Mcy1"
     CDS             complement(5544..5612)
                     /locus_tag="LacZ alpha"
     misc_feature    990..1019
                     /locus_tag="Mcy2"
     misc_binding    complement(2906..2928)
                     /locus_tag="LacO"
     misc_feature    1026..1055
                     /locus_tag="Mcy3"
     CDS             complement(4048..4707)
                     /locus_tag="AmpR"
     misc_feature    1764..1793
                     /locus_tag="Myc4"
     misc_feature    1800..1832
                     /locus_tag="Myc5"
     CDS             1062..1757
                     /locus_tag="mScarlet-I"
ORIGIN
        1 GGGCGAATTG GGCCCgtata atgcagacct gctgcAAGCT AGGGGCACAG CAAGGTCTTC
       61 TGAAATTCAT GTTTTTTTTT TTTTTACTCT GCATTGCAGT CTCCGCTCTT ATTTAGTTTT
      121 GCTTTACGTA AGGTCTCGTT GCTGCCATAA AATAAGCTCT AGTGgaattc ATGCCTTTGT
      181 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT
      241 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG
      301 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG
      361 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA
      421 TCTTGAGCCC CTGCGGACGG TGcCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG
      481 CcATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT
      541 CTGGTTATGT GTGGGAGGGC TAAccatggT GCCCATTTAG TTGGCTTTTC CCTTGTCTCG
      601 TGTCTTTTCC GTGGAAAGGT TCCCGGAGTA ATCTGATGGC ACAGCAGGGA GGTGCGCCTG
      661 CAGGTTGGTT AGGAAGGGGG GATGATGTAA AAGAAGAAAA TGGGGGGATT CGAGCCCCac
      721 gcgtTGCTCA AGCTGTGTAG CGCAgGCGTT TCCTTACATA TTTCTCTAAC AGGCACGGAA
      781 GCCTAACAAA TACACTTGGC TTATTTTTTT GCCCCCTCAT GTCTTGTACA AATATTTGCG
      841 ATAGCTTAGC TATCAGCCAC ATTAATCAAA CAAGTATACC AACAAGCCCG AAAACAGAAA
      901 CTCAACTGCA ACGggtaccT ATGGGTTCTG GTAGTGGTTC CGGTTCCGGT TCTGAGCAGA
      961 AGCTGATAAG TGAAGAAGAC TTGGGCTCCG AGCAAAAGCT GATTTCGGAA GAGGATCTGG
     1021 GCTCTGAGCA GAAGCTGATT TCTGAAGAGG ATCTTaagct tATGGTATCA AAGGGCGAAG
     1081 CAGTAATCAA GGAGTTCATG CGGTTTAAGG TACACATGGA GGGTTCCATG AATGGCCATG
     1141 AATTTGAGAT CGAAGGAGAG GGGGAAGGGC GTCCTTACGA AGGAACACAA ACAGCTAAAC
     1201 TTAAGGTAAC TAAGGGGGGA CCCCTCCCCT TTTCCTGGGA CATACTGTCA CCACAATTCA
     1261 TGTACGGCAG CCGAGCCTTT ATAAAACATC CAGCTGATAT ACCCGATTAT TATAAGCAAA
     1321 GTTTTCCCGA GGGCTTTAAG TGGGAAAGGG TCATGAACTT CGAGGACGGG GGTGCGGTAA
     1381 CTGTAACCCA AGACACCTCT CTCGAAGACG GGACGCTTAT TTATAAGGTG AAACTCCGGG
     1441 GGACCAATTT TCCCCCTGAC GGACCTGTCA TGCAGAAGAA GACAATGGGC TGGGAGGCGT
     1501 CCACAGAGCG CCTGTATCCG GAGGATGGCG TACTTAAGGG TGACATAAAA ATGGCACTTC
     1561 GTTTGAAGGA CGGTGGCCGT TATCTCGCTG ACTTTAAGAC AACTTATAAG GCGAAAAAGC
     1621 CGGTACAGAT GCCAGGAGCA TACAATGTTG ACCGCAAATT GGACATCACC AGCCACAACG
     1681 AAGACTATAC CGTCGTTGAA CAGTACGAAC GGAGTGAAGG GAGGCACAGT ACAGGCGGGA
     1741 TGGACGAATT GTATAAAgga tccGAGCAGA AGCTGATAAG CGAGGAAGAC CTGGGTAGTG
     1801 AACAGAAGCT GATCTCAGAA GAGGACCTTG GCTCAGAACA AAAACTCATC TCAGAGGAAG
     1861 ATCTTGGATC AGGATCTGGA TCAGGATCGG GTAGTTAAga gctcCGCTGC GCTTAAATGT
     1921 CTTTCATTAT AATCAATGTA TAACCTTTAT GTAGTATTTC AATCTATGCC GCTGTGTACG
     1981 TGCACTGCGG TGCCTATCCT TCGGCATTAG AGAGTCACTG TTTGTGTAGA TCGTAGCTGC
     2041 ATGTCTGACA TCTGGTTCAT TAGTGCCGTT TTGAGTCTGC ACTGTTGTGC AACTTGATAT
     2101 TCATCACTGC ACACCAACCG TGTGTTTCCT CCTTTGGGTG CACCCTTTAA TCCTTGTCTT
     2161 CTCCTTTTTT GTCTCTTTCC CCCTCGAAAA GGTGTCAAAC TACTGCCGCA TAAACTACGG
     2221 TTATCCCAAA TTTAAGAGAA AGCAATAAAG CATCAgctag cnATGCCTTT GTCTCAAGAA
     2281 GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC
     2341 TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA
     2401 TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG
     2461 GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC
     2521 CCCTGCGGAC GGTGcCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA AGCcATAGTG
     2581 AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT
     2641 GTGTGGGAGG GCTAActcga gTGCCCATTT AGTTGGCTTT TCCCTTGTCT CGTGTCTTTT
     2701 CCGTGGAAAG GTTCCCGGAG TAATCTGATG GCACAGCAGG GAGGTGCGCC TGCAGGTTGG
     2761 TTAGGAAGGG GGGATGATGT AAAAGAAGAA AATGGGGGGA TTCGAGCCCC GCACAGGTCT
     2821 CTCAAATTGG ATGCATAGCT TGAGTATTCT ATAGTGTCAC CTAAATAGCT TGGCGTAATC
     2881 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG
     2941 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT
     3001 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG
     3061 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT
     3121 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC
     3181 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG
     3241 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
     3301 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     3361 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     3421 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     3481 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     3541 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     3601 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     3661 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     3721 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     3781 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     3841 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
     3901 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
     3961 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
     4021 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
     4081 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
     4141 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
     4201 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
     4261 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
     4321 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
     4381 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
     4441 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
     4501 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
     4561 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
     4621 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
     4681 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
     4741 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
     4801 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
     4861 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
     4921 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
     4981 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGATGC
     5041 GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGAAA TTGTAAGCGT
     5101 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA
     5161 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT
     5221 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG
     5281 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT
     5341 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
     5401 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG
     5461 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT
     5521 TAATGCGCCG CTACAGGGCG CGTCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG
     5581 CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG
     5641 CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT
     5701 GAATTGTAAT ACGACTCACT ATA   
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