LOCUS       D9_pPOTv7_hygro_        6382 bp ds-DNA     circular     23-DEC-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     C terminal tag:
COMMENT     For: ggttctggtagtggttcc
COMMENT     Rev: ccaatttgagagacctgtgc
COMMENT     
COMMENT     N terminal tag
COMMENT     For: gtataatgcagacctgctgc
COMMENT     Rev: actacccgatcctgatcc
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     5'UTR           36..164
                     /locus_tag="Actin 5'"
     misc_feature    1893..1922
                     /locus_tag="GS linker"
     CDS             join(2289..2536,2538..2641,2643..2737,2739..3067,
                     3069..3311)
                     /locus_tag="HYG"
     CDS             join(171..418,420..523,525..619,621..949,951..1193)
                     /locus_tag="HYG(1)"
     5'UTR           join(1358..1377,1379..1546)
                     /locus_tag="Aldolase 5'"
     primer_bind     16..35
                     /locus_tag="#161"
     3'UTR           1203..1351
                     /locus_tag="Ald3'UTR (short)"
     primer_bind     complement(3470..3489)
                     /locus_tag="#162"
     3'UTR           3321..3469
                     /locus_tag="Ald3'UTR (short)(1)"
     primer_bind     6342..6359
                     /locus_tag="M13-fwd"
     primer_bind     complement(3539..3559)
                     /locus_tag="M13-rev"
     primer_bind     join(6358..6382,1..3)
                     /locus_tag="T7"
     primer_bind     complement(3508..3533)
                     /locus_tag="SP6"
     rep_origin      complement(3927..4609)
                     /locus_tag="ColE1 origin"
     rep_origin      complement(5879..6185)
                     /locus_tag="F1 ori"
     CDS             complement(6203..6271)
                     /locus_tag="LacZ alpha"
     misc_feature    1557..1586
                     /locus_tag="GS linker(1)"
     misc_binding    complement(3565..3587)
                     /locus_tag="LacO"
     CDS             complement(4707..5366)
                     /locus_tag="AmpR"
     3'UTR           join(1932..1943,1945..2127,2129..2282)
                     /locus_tag="PFR2 IGS"
     CDS             1593..1886
                     /locus_tag="10xFLAG"
ORIGIN
        1 GGGCGAATTG GGCCCgtata atgcagacct gctgcAAGCT AGGGGCACAG CAAGGTCTTC
       61 TGAAATTCAT GTTTTTTTTT TTTTTACTCT GCATTGCAGT CTCCGCTCTT ATTTAGTTTT
      121 GCTTTACGTA AGGTCTCGTT GCTGCCATAA AATAAGCTCT AGTGgaattc ATGAAAAAGC
      181 CTGAACTCAC CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG
      241 ACCTGATGCA GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC
      301 GTGGATATGT CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT
      361 ATCGGCACTT TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTtA
      421 GCGAGAGCCT GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC
      481 CTGAAACCGA ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCaATGGAT GCGATCGCTG
      541 CGGCCGATCT TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT
      601 ACACTACATG GCGTGATTTt ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA
      661 CTGTGATGGA CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT
      721 GGGCCGAGGA CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG
      781 TCCTGACGGA CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG
      841 ATTCCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC
      901 AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCt CGGCTCCGGG
      961 CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC GGCAATTTCG
     1021 ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA GCCGGGACTG
     1081 TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC TGTGTAGAAG
     1141 TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG GAATAGccat
     1201 ggTGCCCATT TAGTTGGCTT TTCCCTTGTC TCGTGTCTTT TCCGTGGAAA GGTTCCCGGA
     1261 GTAATCTGAT GGCACAGCAG GGAGGTGCGC CTGCAGGTTG GTTAGGAAGG GGGGATGATG
     1321 TAAAAGAAGA AAATGGGGGG ATTCGAGCCC CacgcgtTGC TCAAGCTGTG TAGCGCAgGC
     1381 GTTTCCTTAC ATATTTCTCT AACAGGCACG GAAGCCTAAC AAATACACTT GGCTTATTTT
     1441 TTTGCCCCCT CATGTCTTGT ACAAATATTT GCGATAGCTT AGCTATCAGC CACATTAATC
     1501 AAACAAGTAT ACCAACAAGC CCGAAAACAG AAACTCAACT GCAACGggta ccTATGGGTT
     1561 CTGGTAGTGG TTCCGGTTCC GGTTCTaagc ttGACTATAA AGATGACGAT GACAAGGGGT
     1621 CCGACTACAA GGATGACGAT GACAAGGGCA GTGACTATAA GGACGATGAT GATAAAGGAT
     1681 CAGATTACAA AGACGACGAC GATAAGGGCT CAGACTACAA AGATGACGAT GACAAAGGGA
     1741 GCGATTACAA GGATGACGAT GATAAAGGAT CAGACTACAA GGACGACGAC GATAAAGGGA
     1801 GTGACTACAA AGATGATGAT GACAAGGGTT CGGATTACAA AGACGACGAT GACAAGGGGA
     1861 GCGATTACAA GGATGATGAC GATAAGggat ccGGATCAGG ATCTGGATCA GGATCGGGTA
     1921 GTTAAgagct cCGCTGCGCT TAAATGTCTT TCATTATAAT CAATGTATAA CCTTTATGTA
     1981 GTATTTCAAT CTATGCCGCT GTGTACGTGC ACTGCGGTGC CTATCCTTCG GCATTAGAGA
     2041 GTCACTGTTT GTGTAGATCG TAGCTGCATG TCTGACATCT GGTTCATTAG TGCCGTTTTG
     2101 AGTCTGCACT GTTGTGCAAC TTGATATTCA TCACTGCACA CCAACCGTGT GTTTCCTCCT
     2161 TTGGGTGCAC CCTTTAATCC TTGTCTTCTC CTTTTTTGTC TCTTTCCCCC TCGAAAAGGT
     2221 GTCAAACTAC TGCCGCATAA ACTACGGTTA TCCCAAATTT AAGAGAAAGC AATAAAGCAT
     2281 CAgctagcAT GAAAAAGCCT GAACTCACCG CGACGTCTGT CGAGAAGTTT CTGATCGAAA
     2341 AGTTCGACAG CGTCTCCGAC CTGATGCAGC TCTCGGAGGG CGAAGAATCT CGTGCTTTCA
     2401 GCTTCGATGT AGGAGGGCGT GGATATGTCC TGCGGGTAAA TAGCTGCGCC GATGGTTTCT
     2461 ACAAAGATCG TTATGTTTAT CGGCACTTTG CATCGGCCGC GCTCCCGATT CCGGAAGTGC
     2521 TTGACATTGG GGAATTtAGC GAGAGCCTGA CCTATTGCAT CTCCCGCCGT GCACAGGGTG
     2581 TCACGTTGCA AGACCTGCCT GAAACCGAAC TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG
     2641 CaATGGATGC GATCGCTGCG GCCGATCTTA GCCAGACGAG CGGGTTCGGC CCATTCGGAC
     2701 CGCAAGGAAT CGGTCAATAC ACTACATGGC GTGATTTtAT ATGCGCGATT GCTGATCCCC
     2761 ATGTGTATCA CTGGCAAACT GTGATGGACG ACACCGTCAG TGCGTCCGTC GCGCAGGCTC
     2821 TCGATGAGCT GATGCTTTGG GCCGAGGACT GCCCCGAAGT CCGGCACCTC GTGCACGCGG
     2881 ATTTCGGCTC CAACAATGTC CTGACGGACA ATGGCCGCAT AACAGCGGTC ATTGACTGGA
     2941 GCGAGGCGAT GTTCGGGGAT TCCCAATACG AGGTCGCCAA CATCTTCTTC TGGAGGCCGT
     3001 GGTTGGCTTG TATGGAGCAG CAGACGCGCT ACTTCGAGCG GAGGCATCCG GAGCTTGCAG
     3061 GATCGCCtCG GCTCCGGGCG TATATGCTCC GCATTGGTCT TGACCAACTC TATCAGAGCT
     3121 TGGTTGACGG CAATTTCGAT GATGCAGCTT GGGCGCAGGG TCGATGCGAC GCAATCGTCC
     3181 GATCCGGAGC CGGGACTGTC GGGCGTACAC AAATCGCCCG CAGAAGCGCG GCCGTCTGGA
     3241 CCGATGGCTG TGTAGAAGTA CTCGCCGATA GTGGAAACCG ACGCCCCAGC ACTCGTCCGA
     3301 GGGCAAAGGA ATAGctcgag TGCCCATTTA GTTGGCTTTT CCCTTGTCTC GTGTCTTTTC
     3361 CGTGGAAAGG TTCCCGGAGT AATCTGATGG CACAGCAGGG AGGTGCGCCT GCAGGTTGGT
     3421 TAGGAAGGGG GGATGATGTA AAAGAAGAAA ATGGGGGGAT TCGAGCCCCG CACAGGTCTC
     3481 TCAAATTGGA TGCATAGCTT GAGTATTCTA TAGTGTCACC TAAATAGCTT GGCGTAATCA
     3541 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA
     3601 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT
     3661 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA
     3721 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC
     3781 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
     3841 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC
     3901 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
     3961 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
     4021 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
     4081 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
     4141 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
     4201 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
     4261 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
     4321 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
     4381 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
     4441 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
     4501 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
     4561 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     4621 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     4681 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     4741 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     4801 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG
     4861 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     4921 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     4981 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     5041 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     5101 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     5161 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     5221 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     5281 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     5341 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     5401 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     5461 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     5521 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     5581 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     5641 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGATGCG
     5701 GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGAAAT TGTAAGCGTT
     5761 AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG
     5821 GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT
     5881 GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA
     5941 AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG
     6001 GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT
     6061 TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC
     6121 GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT
     6181 AATGCGCCGC TACAGGGCGC GTCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC
     6241 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC
     6301 GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG
     6361 AATTGTAATA CGACTCACTA TA   
//
