LOCUS       E2_pPOTv7_blast_        5528 bp ds-DNA     circular     23-DEC-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     C terminal tag:
COMMENT     For: ggttctggtagtggttcc
COMMENT     Rev: ccaatttgagagacctgtgc
COMMENT     
COMMENT     N terminal tag
COMMENT     For: gtataatgcagacctgctgc
COMMENT     Rev: actacccgatcctgatcc
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             join(171..442,444..481,483..560)
                     /locus_tag="BLAST"
     CDS             join(2068..2339,2341..2378,2380..2457)
                     /locus_tag="BLAST(1)"
     misc_feature    1671..1700
                     /locus_tag="GS linker"
     5'UTR           36..164
                     /locus_tag="Actin 5'"
     5'UTR           join(725..744,746..913)
                     /locus_tag="Aldolase 5'"
     primer_bind     16..35
                     /locus_tag="#161"
     primer_bind     complement(2616..2635)
                     /locus_tag="#162"
     3'UTR           570..718
                     /locus_tag="Ald3'UTR (short)"
     3'UTR           join(1710..1721,1723..1905,1907..2060)
                     /locus_tag="PFR2 IGS"
     3'UTR           2467..2615
                     /locus_tag="Ald3'UTR (short)(1)"
     primer_bind     5488..5505
                     /locus_tag="M13-fwd"
     primer_bind     complement(2685..2705)
                     /locus_tag="M13-rev"
     primer_bind     join(5504..5528,1..3)
                     /locus_tag="T7"
     primer_bind     complement(2654..2679)
                     /locus_tag="SP6"
     rep_origin      complement(3073..3755)
                     /locus_tag="ColE1 origin"
     CDS             960..1664
                     /locus_tag="mNG"
     rep_origin      complement(5025..5331)
                     /locus_tag="F1 ori"
     CDS             complement(5349..5417)
                     /locus_tag="LacZ alpha"
     misc_binding    complement(2711..2733)
                     /locus_tag="LacO"
     CDS             complement(3853..4512)
                     /locus_tag="AmpR"
     misc_feature    924..953
                     /locus_tag="GS linker(1)"
ORIGIN
        1 GGGCGAATTG GGCCCgtata atgcagacct gctgcAAGCT AGGGGCACAG CAAGGTCTTC
       61 TGAAATTCAT GTTTTTTTTT TTTTTACTCT GCATTGCAGT CTCCGCTCTT ATTTAGTTTT
      121 GCTTTACGTA AGGTCTCGTT GCTGCCATAA AATAAGCTCT AGTGgaattc ATGCCTTTGT
      181 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT
      241 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG
      301 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG
      361 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA
      421 TCTTGAGCCC CTGCGGACGG TGcCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG
      481 CcATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT
      541 CTGGTTATGT GTGGGAGGGC TAAccatggT GCCCATTTAG TTGGCTTTTC CCTTGTCTCG
      601 TGTCTTTTCC GTGGAAAGGT TCCCGGAGTA ATCTGATGGC ACAGCAGGGA GGTGCGCCTG
      661 CAGGTTGGTT AGGAAGGGGG GATGATGTAA AAGAAGAAAA TGGGGGGATT CGAGCCCCac
      721 gcgtTGCTCA AGCTGTGTAG CGCAgGCGTT TCCTTACATA TTTCTCTAAC AGGCACGGAA
      781 GCCTAACAAA TACACTTGGC TTATTTTTTT GCCCCCTCAT GTCTTGTACA AATATTTGCG
      841 ATAGCTTAGC TATCAGCCAC ATTAATCAAA CAAGTATACC AACAAGCCCG AAAACAGAAA
      901 CTCAACTGCA ACGggtaccT ATGGGTTCTG GTAGTGGTTC CGGTTCCGGT TCTaagcttG
      961 TCTCGAAAGG TGAGGAAGAC AATATGGCCA GCTTGCCAGC AACGCACGAG CTTCATATAT
     1021 TTGGGTCGAT CAACGGTGTG GATTTCGACA TGGTGGGTCA AGGCACAGGT AATCCTAATG
     1081 ACGGGTATGA AGAACTCAAT TTGAAGAGCA CTAAGGGAGA TCTCCAGTTT TCCCCGTGGA
     1141 TTCTTGTGCC GCATATTGGT TATGGTTTTC ATCAGTACTT ACCATACCCC GATGGAATGT
     1201 CGCCCTTCCA AGCAGCTATG GTGGACGGCT CGGGGTATCA AGTACATCGC ACCATGCAAT
     1261 TTGAGGACGG AGCATCCCTC ACCGTAAACT ACCGCTATAC GTATGAGGGG AGCCACATAA
     1321 AAGGTGAGGC TCAAGTTAAA GGCACAGGCT TCCCAGCTGA CGGCCCGGTC ATGACCAATT
     1381 CATTAACCGC TGCTGATTGG TGCCGTTCTA AGAAGACCTA CCCTAACGAC AAGACGATCA
     1441 TTAGTACTTT TAAGTGGAGT TATACCACAG GAAACGGAAA GCGGTATCGA AGCACTGCTC
     1501 GAACAACCTA CACATTTGCG AAGCCAATGG CCGCGAACTA CTTGAAGAAC CAGCCAATGT
     1561 ATGTGTTTCG AAAAACGGAA CTGAAGCATT CGAAAACCGA GCTTAATTTT AAAGAGTGGC
     1621 AGAAGGCGTT TACAGACGTG ATGGGGATGG ACGAATTGTA TAAGggatcc GGATCAGGAT
     1681 CTGGATCAGG ATCGGGTAGT TAAgagctcC GCTGCGCTTA AATGTCTTTC ATTATAATCA
     1741 ATGTATAACC TTTATGTAGT ATTTCAATCT ATGCCGCTGT GTACGTGCAC TGCGGTGCCT
     1801 ATCCTTCGGC ATTAGAGAGT CACTGTTTGT GTAGATCGTA GCTGCATGTC TGACATCTGG
     1861 TTCATTAGTG CCGTTTTGAG TCTGCACTGT TGTGCAACTT GATATTCATC ACTGCACACC
     1921 AACCGTGTGT TTCCTCCTTT GGGTGCACCC TTTAATCCTT GTCTTCTCCT TTTTTGTCTC
     1981 TTTCCCCCTC GAAAAGGTGT CAAACTACTG CCGCATAAAC TACGGTTATC CCAAATTTAA
     2041 GAGAAAGCAA TAAAGCATCA gctagcnATG CCTTTGTCTC AAGAAGAATC CACCCTCATT
     2101 GAAAGAGCAA CGGCTACAAT CAACAGCATC CCCATCTCTG AAGACTACAG CGTCGCCAGC
     2161 GCAGCTCTCT CTAGCGACGG CCGCATCTTC ACTGGTGTCA ATGTATATCA TTTTACTGGG
     2221 GGACCTTGTG CAGAACTCGT GGTGCTGGGC ACTGCTGCTG CTGCGGCAGC TGGCAACCTG
     2281 ACTTGTATCG TCGCGATCGG AAATGAGAAC AGGGGCATCT TGAGCCCCTG CGGACGGTGc
     2341 CGACAGGTGC TTCTCGATCT GCATCCTGGG ATCAAAGCcA TAGTGAAGGA CAGTGATGGA
     2401 CAGCCGACGG CAGTTGGGAT TCGTGAATTG CTGCCCTCTG GTTATGTGTG GGAGGGCTAA
     2461 ctcgagTGCC CATTTAGTTG GCTTTTCCCT TGTCTCGTGT CTTTTCCGTG GAAAGGTTCC
     2521 CGGAGTAATC TGATGGCACA GCAGGGAGGT GCGCCTGCAG GTTGGTTAGG AAGGGGGGAT
     2581 GATGTAAAAG AAGAAAATGG GGGGATTCGA GCCCCGCACA GGTCTCTCAA ATTGGATGCA
     2641 TAGCTTGAGT ATTCTATAGT GTCACCTAAA TAGCTTGGCG TAATCATGGT CATAGCTGTT
     2701 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA
     2761 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT
     2821 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC
     2881 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG
     2941 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
     3001 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
     3061 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     3121 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     3181 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     3241 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     3301 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     3361 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     3421 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     3481 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     3541 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     3601 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     3661 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
     3721 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA
     3781 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG
     3841 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG
     3901 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC
     3961 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC
     4021 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC
     4081 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG
     4141 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT
     4201 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG
     4261 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT
     4321 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG
     4381 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG
     4441 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT
     4501 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT
     4561 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC
     4621 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT
     4681 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT
     4741 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA
     4801 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GATGCGGTGT GAAATACCGC
     4861 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGAAATTGTA AGCGTTAATA TTTTGTTAAA
     4921 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA
     4981 AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA
     5041 CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA
     5101 GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG
     5161 TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC
     5221 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC
     5281 AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA
     5341 GGGCGCGTCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
     5401 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
     5461 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT GTAATACGAC
     5521 TCACTATA     
//
