LOCUS       pPOTv7_blast_bla        6977 bp ds-DNA     circular     21-MAR-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     C terminal tag:
COMMENT     For: ggttctggtagtggttcc
COMMENT     Rev: ccaatttgagagacctgtgc
COMMENT     
COMMENT     
COMMENT     N terminal tag
COMMENT     For: gtataatgcagacctgctgc
COMMENT     Rev: actacccgatcctgatcc
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             join(171..442,444..481,483..560)
                     /label=BLAST
     CDS             join(3517..3788,3790..3827,3829..3906)
                     /label=BLAST(1)
     CDS             1686..2390
                     /label=mNG ORF
     misc_feature    3120..3149
                     /label=GS linker
     5'UTR           36..164
                     /label=Actin 5'
     CDS             2409..3113
                     /label=mNG ORF(1)
     5'UTR           join(725..744,746..913)
                     /label=Aldolase 5'
     primer_bind     16..35
                     /label=#161
     primer_bind     complement(4065..4084)
                     /label=#162
     3'UTR           570..718
                     /label=Ald3'UTR (short)
     3'UTR           join(3159..3170,3172..3354,3356..3509)
                     /label=PFR2 IGS
     3'UTR           3916..4064
                     /label=Ald3'UTR (short)(1)
     primer_bind     6937..6954
                     /label=M13-fwd
     primer_bind     complement(4134..4154)
                     /label=M13-rev
     primer_bind     join(6953..6977,1..3)
                     /label=T7
     primer_bind     complement(4103..4128)
                     /label=SP6
     rep_origin      complement(4522..5204)
                     /label=ColE1 origin
     rep_origin      complement(6474..6780)
                     /label=F1 ori
     CDS             complement(6798..6866)
                     /label=LacZ alpha
     misc_binding    complement(4160..4182)
                     /label=LacO
     CDS             complement(5302..5961)
                     /label=AmpR
     misc_feature    924..953
                     /label=GS linker(1)
     CDS             960..1664
                     /label=mNeonGreen
ORIGIN
        1 GGGCGAATTG GGCCCgtata atgcagacct gctgcAAGCT AGGGGCACAG CAAGGTCTTC
       61 TGAAATTCAT GTTTTTTTTT TTTTTACTCT GCATTGCAGT CTCCGCTCTT ATTTAGTTTT
      121 GCTTTACGTA AGGTCTCGTT GCTGCCATAA AATAAGCTCT AGTGgaattc ATGCCTTTGT
      181 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT
      241 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG
      301 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG
      361 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA
      421 TCTTGAGCCC CTGCGGACGG TGcCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG
      481 CcATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT
      541 CTGGTTATGT GTGGGAGGGC TAAccatggT GCCCATTTAG TTGGCTTTTC CCTTGTCTCG
      601 TGTCTTTTCC GTGGAAAGGT TCCCGGAGTA ATCTGATGGC ACAGCAGGGA GGTGCGCCTG
      661 CAGGTTGGTT AGGAAGGGGG GATGATGTAA AAGAAGAAAA TGGGGGGATT CGAGCCCCac
      721 gcgtTGCTCA AGCTGTGTAG CGCAgGCGTT TCCTTACATA TTTCTCTAAC AGGCACGGAA
      781 GCCTAACAAA TACACTTGGC TTATTTTTTT GCCCCCTCAT GTCTTGTACA AATATTTGCG
      841 ATAGCTTAGC TATCAGCCAC ATTAATCAAA CAAGTATACC AACAAGCCCG AAAACAGAAA
      901 CTCAACTGCA ACGggtaccT ATGGGTTCTG GTAGTGGTTC CGGTTCCGGT TCTaagcttG
      961 TCTCGAAAGG TGAGGAAGAC AATATGGCCA GCTTGCCAGC AACGCACGAG CTTCATATAT
     1021 TTGGGTCGAT CAACGGTGTG GATTTCGACA TGGTGGGTCA AGGCACAGGT AATCCTAATG
     1081 ACGGGTATGA AGAACTCAAT TTGAAGAGCA CTAAGGGAGA TCTCCAGTTT TCCCCGTGGA
     1141 TTCTTGTGCC GCATATTGGT TATGGTTTTC ATCAGTACTT ACCATACCCC GATGGAATGT
     1201 CGCCCTTCCA AGCAGCTATG GTGGACGGCT CGGGGTATCA AGTACATCGC ACCATGCAAT
     1261 TTGAGGACGG AGCATCCCTC ACCGTAAACT ACCGCTATAC GTATGAGGGG AGCCACATAA
     1321 AAGGTGAGGC TCAAGTTAAA GGCACAGGCT TCCCAGCTGA CGGCCCGGTC ATGACCAATT
     1381 CATTAACCGC TGCTGATTGG TGCCGTTCTA AGAAGACCTA CCCTAACGAC AAGACGATCA
     1441 TTAGTACTTT TAAGTGGAGT TATACCACAG GAAACGGAAA GCGGTATCGA AGCACTGCTC
     1501 GAACAACCTA CACATTTGCG AAGCCAATGG CCGCGAACTA CTTGAAGAAC CAGCCAATGT
     1561 ATGTGTTTCG AAAAACGGAA CTGAAGCATT CGAAAACCGA GCTTAATTTT AAAGAGTGGC
     1621 AGAAGGCGTT TACAGACGTG ATGGGGATGG ACGAATTGTA TAAGCCCGGG ggcggctccg
     1681 gaagcGTGTC CAAGGGTGAG GAAGATAACA TGGCCTCTCT GCCCGCCACC CACGAGTTGC
     1741 ATATCTTTGG AAGCATTAAC GGAGTGGATT TCGATATGGT GGGTCAGGGG ACAGGAAATC
     1801 CGAACGATGG TTATGAAGAG TTGAACTTGA AAAGCACGAA GGGAGACCTC CAATTTTCGC
     1861 CCTGGATCTT GGTGCCGCAT ATCGGATATG GGTTCCATCA ATATTTGCCC TACCCTGACG
     1921 GGATGTCACC CTTTCAGGCC GCGATGGTTG ATGGTTCGGG CTATCAGGTA CACCGGACAA
     1981 TGCAATTTGA AGATGGTGCC TCTTTGACAG TCAATTACCG GTACACCTAT GAAGGGAGCC
     2041 ATATTAAAGG TGAAGCCCAA GTGAAGGGCA CCGGCTTCCC TGCGGACGGG CCTGTTATGA
     2101 CGAACTCACT GACTGCGGCA GATTGGTGTA GGTCGAAGAA GACTTATCCA AATGACAAAA
     2161 CAATAATCTC CACATTTAAG TGGTCGTACA CAACAGGAAA TGGTAAGCGT TACCGCTCGA
     2221 CTGCGAGGAC GACCTACACA TTCGCCAAGC CTATGGCGGC CAACTATCTC AAGAATCAAC
     2281 CTATGTACGT GTTCCGGAAA ACTGAGTTGA AACACTCTAA AACCGAACTC AACTTCAAGG
     2341 AATGGCAAAA AGCGTTTACG GATGTAATGG GAATGGATGA ATTGTACAAG ggctccggaa
     2401 gcggcagcGT ATCTAAGGGT GAAGAGGATA ATATGGCGAG CCTGCCAGCC ACTCACGAGC
     2461 TTCACATTTT CGGGTCCATA AATGGCGTAG ACTTTGACAT GGTAGGACAG GGGACGGGAA
     2521 ACCCTAACGA TGGCTATGAG GAGCTTAACT TGAAAAGCAC GAAGGGAGAT CTGCAATTCT
     2581 CTCCGTGGAT TCTCGTTCCA CACATAGGTT ATGGGTTTCA TCAGTACCTC CCCTACCCAG
     2641 ACGGCATGAG TCCGTTCCAA GCAGCAATGG TCGATGGTTC TGGCTATCAA GTCCACCGTA
     2701 CTATGCAGTT TGAAGATGGA GCGAGCCTCA CGGTAAATTA TCGTTATACC TACGAAGGGT
     2761 CTCATATTAA GGGTGAAGCC CAGGTGAAGG GGACCGGATT CCCGGCCGAT GGCCCTGTGA
     2821 TGACGAATAG CCTTACGGCC GCTGACTGGT GTCGGAGTAA AAAGACCTAT CCCAATGACA
     2881 AGACAATTAT TTCCACGTTT AAATGGTCAT ATACGACTGG GAATGGTAAG CGATACCGAT
     2941 CCACTGCCCG GACGACTTAT ACCTTTGCTA AGCCTATGGC GGCGAACTAC CTCAAGAATC
     3001 AGCCAATGTA CGTGTTCCGA AAGACAGAGC TGAAACACTC GAAGACTGAA CTCAATTTTA
     3061 AAGAATGGCA AAAGGCCTTC ACCGATGTGA TGGGAATGGA TGAACTGTAT AAAggatccG
     3121 GATCAGGATC TGGATCAGGA TCGGGTAGTT AAgagctcCG CTGCGCTTAA ATGTCTTTCA
     3181 TTATAATCAA TGTATAACCT TTATGTAGTA TTTCAATCTA TGCCGCTGTG TACGTGCACT
     3241 GCGGTGCCTA TCCTTCGGCA TTAGAGAGTC ACTGTTTGTG TAGATCGTAG CTGCATGTCT
     3301 GACATCTGGT TCATTAGTGC CGTTTTGAGT CTGCACTGTT GTGCAACTTG ATATTCATCA
     3361 CTGCACACCA ACCGTGTGTT TCCTCCTTTG GGTGCACCCT TTAATCCTTG TCTTCTCCTT
     3421 TTTTGTCTCT TTCCCCCTCG AAAAGGTGTC AAACTACTGC CGCATAAACT ACGGTTATCC
     3481 CAAATTTAAG AGAAAGCAAT AAAGCATCAg ctagcnATGC CTTTGTCTCA AGAAGAATCC
     3541 ACCCTCATTG AAAGAGCAAC GGCTACAATC AACAGCATCC CCATCTCTGA AGACTACAGC
     3601 GTCGCCAGCG CAGCTCTCTC TAGCGACGGC CGCATCTTCA CTGGTGTCAA TGTATATCAT
     3661 TTTACTGGGG GACCTTGTGC AGAACTCGTG GTGCTGGGCA CTGCTGCTGC TGCGGCAGCT
     3721 GGCAACCTGA CTTGTATCGT CGCGATCGGA AATGAGAACA GGGGCATCTT GAGCCCCTGC
     3781 GGACGGTGcC GACAGGTGCT TCTCGATCTG CATCCTGGGA TCAAAGCcAT AGTGAAGGAC
     3841 AGTGATGGAC AGCCGACGGC AGTTGGGATT CGTGAATTGC TGCCCTCTGG TTATGTGTGG
     3901 GAGGGCTAAc tcgagTGCCC ATTTAGTTGG CTTTTCCCTT GTCTCGTGTC TTTTCCGTGG
     3961 AAAGGTTCCC GGAGTAATCT GATGGCACAG CAGGGAGGTG CGCCTGCAGG TTGGTTAGGA
     4021 AGGGGGGATG ATGTAAAAGA AGAAAATGGG GGGATTCGAG CCCCGCACAG GTCTCTCAAA
     4081 TTGGATGCAT AGCTTGAGTA TTCTATAGTG TCACCTAAAT AGCTTGGCGT AATCATGGTC
     4141 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG
     4201 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT
     4261 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG
     4321 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA
     4381 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT
     4441 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA
     4501 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC
     4561 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     4621 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     4681 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     4741 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     4801 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     4861 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     4921 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     4981 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     5041 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     5101 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     5161 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT
     5221 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA
     5281 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     5341 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     5401 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC
     5461 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     5521 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     5581 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC
     5641 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     5701 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     5761 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     5821 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     5881 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     5941 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     6001 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     6061 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     6121 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA
     6181 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     6241 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ATGCGGTGTG
     6301 AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GAAATTGTAA GCGTTAATAT
     6361 TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA
     6421 AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC
     6481 AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC
     6541 CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC
     6601 GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG
     6661 GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG
     6721 GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC
     6781 GCCGCTACAG GGCGCGTCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG
     6841 GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA
     6901 AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTG
     6961 TAATACGACT CACTATA    
//
