LOCUS       Exported               14132 bp DNA     circular SYN 31-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    peYGFPuvGUS
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 14132)
  AUTHORS   Xiaohan Yang
  TITLE     Direct Submission
  JOURNAL   Exported Dec 2, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14132
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     12..31
                     /label=922_cloningR
     primer_bind     complement(12..31)
                     /label=923_cloningF
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(590..614)
                     /label=921_cllningF
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     738..754
                     /label=40_seq_M13  fwd
     misc_feature    750..776
                     /label=overlap
     primer_bind     750..776
                     /label=921_cllningF
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    1599..1623
                     /label=overlap1
     misc_feature    1599..1618
                     /label=OVERLAP
     primer_bind     complement(1599..1618)
                     /label=922_cloningR
     primer_bind     1599..1618
                     /label=923_cloningF
     misc_feature    1624..1730
                     /label=Modified COR47-5'-UTR
     misc_feature    1706..1735
                     /label=overlap
     misc_feature    1731..1758
                     /label=overlap
     misc_feature    1739..2398
                     /label=eYGFPuv
     misc_feature    1739..1763
                     /label=overlap
     primer_bind     1739..1760
                     /label=573_GFPuvF
     misc_feature    1769..1793
                     /label=overlap
     primer_bind     1769..1784
                     /label=523_GFPuvF
     primer_bind     complement(1776..1793)
                     /label=924_cloningR
     primer_bind     complement(2053..2072)
                     /label=472_sequencing
     misc_feature    2299..2323
                     /label=overlap
     primer_bind     complement(2307..2323)
                     /label=524_GFPuvR
     primer_bind     complement(2380..2398)
                     /label=702_GFPuvR
     misc_feature    2399..3276
                     /label=HSPT878
     misc_feature    2424..2448
                     /label=overlap
     primer_bind     complement(2669..2688)
                     /label=471_sequencing
     primer_bind     complement(3250..3277)
                     /label=574_GFPuvR
     primer_bind     complement(3250..3277)
                     /label=705_GFPuvR
     misc_feature    3277..3297
                     /label=overlap
     primer_bind     3299..3316
                     /label=626_cloningF
     primer_bind     complement(3303..3322)
                     /label=VF2R
     misc_feature    3328..3352
                     /label=overlap
     primer_bind     complement(3338..3354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3338..3354)
                     /label=45_seq_M13  rev
     protein_bind    3362..3378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3386..3416)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3431..3452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     3545..3562
                     /label=921_cllningF
     promoter        3643..4320
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4311..4331)
                     /label=922_cloningR
     primer_bind     4350..4370
                     /label=Kan sequence testR
     primer_bind     complement(4352..4369)
                     /label=627_cloningR
     CDS             4388..5185
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     4818..4838
                     /label=KantestR
     primer_bind     complement(5173..5194)
                     /label=Kan sequence testF
     polyA_signal    5225..5399
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5477..5501
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(5543..5563)
                     /label=KantestF
     CDS             5926..6720
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6807..7395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7296..7315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7549..7566
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7581..7721
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7647..7666)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7807..7829
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8065..8259
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8325..9392)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     primer_bind     complement(9174..9186)
                     /label=EGFPCHR
     CDS             complement(9826..10455)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11755..11779
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      11801..12053
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     12044..12068
                     /label=574_GFPuvR
     CDS             complement(12085..12102)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12109..13926,14117..14131))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          13927..14116
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctatcttcat gctgcaacga acggaggtta
     1981 caccaacacc aggaaggaga tctatgaaga cggcggcatc ttggaggtca acttccgtta
     2041 cacttacgag ttcaacaaga tcatcggtga cgtcgagtgc attggacatg gattcccaag
     2101 tcagagtccg atcttcaagg acacgatcgt gaagagttgt cccacggtgg acctgatgtt
     2161 gccaatgtcc gggaacatca tcgccagctc ctacgcttac gccttccaac tgaaggacgg
     2221 ctctttctac acggcagaag tcaagaacaa catagacttc aagaatccaa tccacgagtc
     2281 cttctcgaag tcagggccca tgttcaccca cagacgtgtc gaggagactc tcaccaagga
     2341 gaaccttgcc atagtggagt accagcaggt tttcaacagc gccccaagag acatgtagat
     2401 atgaagatga agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt
     2461 gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt
     2521 aagatctcat tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg
     2581 atctcttctg cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta
     2641 aagataagat gggctcatag agtaaaacga ggcgagggac ctataaacct cccttcatca
     2701 tgctatttca tgatctattt tataaaataa agatgtagaa aaaagtaagc gtaataaccg
     2761 caaaacaaat gatttaaaac atggcacata atgaggagat taagttcggt ttacgtttat
     2821 tttagtacta attgtaacgt gagactacgt atcgggaatc gcctaattaa agcattaatg
     2881 cgaacctgat tagattcacc gaccctccta tcgtgtcgac ctttctgttt cttagaattt
     2941 tttggtagtc tatgtactaa taatgtcagc ttcgtattta tttcataagc aatttgcatt
     3001 tgcaatttgt tttttacttt tatttttatt gtattgtgga atgtggactc gtaccaacat
     3061 gaagttatat accaccaaaa aaattacagt tagtcaaaag attcacgagt gagagctact
     3121 tatgattgtc ttttacgtat atgtctaatt gtctatttgc tcaataatct ttgtactttc
     3181 ttttgtcgtt gataaaatca caaagttcca aaagtaatcg aatgatttgc ttttaagaaa
     3241 agaagagctc aataattcaa catatatctg tacacaatta ccgcctttga gtgagctcta
     3301 gatgccacct gacgtctaag aagaattcgt aatcatggtc atagctgttt cctgtgtgaa
     3361 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     3421 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     3481 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3541 gtttgcgtat tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc
     3601 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
     3661 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
     3721 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
     3781 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     3841 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgataacat
     3901 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
     3961 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
     4021 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
     4081 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
     4141 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4201 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4261 tccttcgcaa gaccttcctc tatataagga agttcatttc atttggagag gacacgctga
     4321 aatcaccagt ctctctctac aaatctatct ctctcgagct ttcgcagatc ccggggggca
     4381 atgagatatg gggattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4441 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4501 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4561 gaatgaactc caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     4621 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     4681 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4741 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4801 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4861 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     4921 catgcccgac ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc cgaatatcat
     4981 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5041 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5101 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5161 tcgccttctt gacgagttct tctgaagtag atgccgaccg gatctgtcga tcgacaagct
     5221 cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag
     5281 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     5341 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc
     5401 cccgaattaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
     5461 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
     5521 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
     5581 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
     5641 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
     5701 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc
     5761 cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta
     5821 tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc
     5881 ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga
     5941 atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga
     6001 atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg
     6061 acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta
     6121 tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg
     6181 agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa
     6241 caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc
     6301 gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac
     6361 ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt
     6421 aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc
     6481 ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc
     6541 tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc
     6601 cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg
     6661 gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga attgttttag
     6721 tacctagaat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6781 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6841 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6901 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6961 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7021 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7081 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7141 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7201 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7261 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7321 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7381 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7441 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7501 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7561 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     7621 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     7681 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     7741 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     7801 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     7861 agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
     7921 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
     7981 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
     8041 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
     8101 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
     8161 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
     8221 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct
     8281 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
     8341 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
     8401 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
     8461 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
     8521 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
     8581 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
     8641 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
     8701 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
     8761 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
     8821 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
     8881 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
     8941 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
     9001 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
     9061 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
     9121 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
     9181 cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg
     9241 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
     9301 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
     9361 ccgatttgta ccgggccgga tggtttgcga ccgtcacgcc gattcctcgg gcttgggggt
     9421 tccagtgcca ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt
     9481 tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc
     9541 gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta
     9601 gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca
     9661 tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg
     9721 tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac
     9781 ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga
     9841 tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc
     9901 aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga
     9961 ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt
    10021 gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat
    10081 gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg
    10141 caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg
    10201 gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat
    10261 cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc
    10321 gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag
    10381 ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac
    10441 agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac
    10501 gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg
    10561 cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa
    10621 ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac
    10681 ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg
    10741 tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt
    10801 cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca
    10861 cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag
    10921 ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg
    10981 ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc
    11041 cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg
    11101 tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca
    11161 ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg
    11221 ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc
    11281 tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc
    11341 agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc
    11401 gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct
    11461 agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg
    11521 gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg
    11581 gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac
    11641 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
    11701 cttttcacgc ccttttaaat atccgttatt ctaataaacg ctcttttctc ttaggtttac
    11761 ccgccaatat atcctgtcaa acactgatag tttaattccc gatctagtaa catagatgac
    11821 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
    11881 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
    11941 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
    12001 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atcggggaaa
    12061 ttcgagctgg tcaccaattc acacgtgatg gtgatggtga tggctagcgt tcttgtagcc
    12121 gaaatctgga atgttggtcc agcgctcgcg aaagacgtgc gcggcgagct tcggcttgcg
    12181 gtcacgagtg aacacgccct tcttgtttcc ttggacgcgc atcacgccct gagaggtcgc
    12241 gaagtccgcg aagttccacg cttgctcacc cacgaagttc tcaaactcat cgaacacgac
    12301 gtggttcgcc tggtagtact cgacttgata ttcctcggtg aacatcactg gatcaatgtc
    12361 gtgaaagccc gcaacggtgt ctgcgccgta ctcagtgatc atgatcggct ttcctgggca
    12421 acgcttgttc cacgcgtgaa attcctggcg gagatggact ttggccgctt cgagatcacc
    12481 gccatcgaag taccatccgt tatagcgatt gagcgcgatg acgtcaatca gttcggcgac
    12541 tttgtccgtc tccggggtag ccatcacaaa cagcacgatc gtgaccggac gcttctgtgg
    12601 gtcgagttcc ttggtcagct ccaccaacgg cttgaagtac tcgtacgcgc cctcttcctc
    12661 agtcgccgcc tcgttggcga tgctccacat cacgacgctt ggatggttct tgtcacgaga
    12721 caccagttca cggagaacgt cttgatggtg ctcaaacgtc cgaatcttct cccaggtact
    12781 gacgcgctcg ctgccttcgc cgagtcccgt ggtggccatg aagttgaggt gcacgccaac
    12841 tgccggagtc tcgtcgatca cgaccagacc ctcgcgatcc gcaagacgca tcaactcttc
    12901 agagtacgga tagtgtgcgg tccggaagct gttggcgccg atccatttga ggatattgaa
    12961 atccatcaca ttgctcgctt cgttaaagcc acggccgttg ataggagtgt cctcatgttt
    13021 gccaaagccc ttgaagtaga acggtttgtt gttgatgagg aacttgccgt cgttgacttc
    13081 cacggtccgc acgccgaacg gctcttcata gacatcgatg gtcagtccgt cgttcaccag
    13141 ttccactttg atctggtaga gatacgtgtt cagtggttcc cagaggatga cattcggaat
    13201 ctccacgtta ccgctcaggc cctcggtgct tgcgaccact ttgccttcct catccacgac
    13261 cgacactttc acggtctcgg ctttgccttg aaagtccacc gtataggtca cagtcccggt
    13321 tgggccattg aagtcggtca caaccgagat gtcctcgacg tacgtaaacg gggtcgtgta
    13381 gattttcacc ggacggtgca ggcctgcata gttgaagaag tcgaagttcg gcttgttacg
    13441 aatgactttt ccgaggccct cttcgtggcg ctcgctgtac agccccaccg ggagggtgct
    13501 atcgtcgagg atgttgtcca cggcgacggt gacgcgattc atgccatcac gcagcgagtt
    13561 gttgatttcc gcttcgaatg gcaggaatcc gcccttgtgc tccacgacca gctcaccatt
    13621 gacatagaca attgctttgt gagttgcaga gccgaagcgg agcacgatac gctgatcctt
    13681 cagataggcc ggcaccgtga actcacgttc gtaccagaca tatccgatat ggttgcggat
    13741 ttccttggtc acgccaatgt cattgtaact gcttgggacg gccatactaa tagtgtcggt
    13801 cagcttgctt tcgtaccact tctcttccag tcctttcccg tagtccagct tgaagttcca
    13861 gacgccattg aggtcgaaga cgccacgggt ctcggtgttg atcgggtaca gactagttcg
    13921 tcggttctgt aactatcatc atcatcatag acacacgaaa taaagtaatc agattatcag
    13981 ttaaagctat gtaatattta caccataacc aatcaattaa aaaatagatc agtttaaaga
    14041 aagatcaaag ctcaaaaaaa taaaaagaga aaagggtcct aaccaagaaa atgaaggaga
    14101 aaaactagaa atttaccctc agatctacca tg
//
