LOCUS       pCDH-AtAFB2-mTagBFP2    8840 bp ds-DNA     circular     29-DEC-2021
DEFINITION  .
KEYWORDS    "Name:pCDH-AtAFB2-mTagBFP2" "Plasmid:pCDH-AtAFB2-mTagBFP2"         
            "Strain/Frozen stock" "Resistance (bacteria)" "Gene or insert"
            "Owner" "Location" "Sub-location" "Location details" "Backbone"
            "Technical details" "Created by" "3' Cloning site - Enzyme Used"
            "3' primer" "5' Cloning site - Enzyme Used" "5' primer"
FEATURES             Location/Qualifiers
     LTR             41..274
                     /label="3' LTR (ΔU3)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     polyA_signal    346..467
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      486..621
                     /label="SV40 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(655..675)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    complement(681..703)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    683..699
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(707..737)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    752..773
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     rep_origin      complement(1043..1725)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(1823..2482)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(3119..3187)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     3258..3275
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     LTR             3518..3698
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3742..3867
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4360..4593
                     /label="RRE"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5082..5199
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5763..7487
                     /label="AtAFB"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             5763..8213
                     /label="Translation 5763-8213"
     CDS             7503..8213
                     /label="mTagBFP2"
                     /ApEinfo_revcolor=#3366ff
                     /ApEinfo_fwdcolor=#3366ff
     misc_feature    8219..8807
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(8690..8701)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC
       61 CAACGAAAAT AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA
      121 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT
      181 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC
      241 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT
      301 CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT GTTTATTGCA
      361 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
      421 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGCTC
      481 TAGCTATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA
      541 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT
      601 GAGGAGGCTT TTTTGGAGGC CTAGACTTTT GCAGAGACGG CCCAAATTCG TAATCATGGT
      661 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG
      721 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT
      781 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG
      841 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG
      901 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA
      961 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC
     1021 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC
     1081 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT
     1141 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC
     1201 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT
     1261 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG
     1321 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC
     1381 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA
     1441 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA
     1501 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA
     1561 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC
     1621 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG
     1681 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA
     1741 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG
     1801 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT
     1861 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG
     1921 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC
     1981 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA
     2041 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC
     2101 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT
     2161 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC
     2221 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT
     2281 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC
     2341 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT
     2401 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA
     2461 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA
     2521 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG
     2581 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA
     2641 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT
     2701 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA
     2761 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG
     2821 AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC
     2881 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
     2941 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
     3001 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
     3061 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
     3121 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
     3181 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
     3241 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTGACGCGT GTAGTCTTAT
     3301 GCAATACTCT TGTAGTCTTG CAACATGGTA ACGATGAGTT AGCAACATGC CTTACAAGGA
     3361 GAGAAAAAGC ACCGTGCATG CCGATTGGTG GAAGTAAGGT GGTACGATCG TGCCTTATTA
     3421 GGAAGGCAAC AGACGGGTCT GACATGGATT GGACGAACCA CTGAATTGCC GCATTGCAGA
     3481 GATATTGTAT TTAAGTGCCT AGCTCGATAC AATAAACGGG TCTCTCTGGT TAGACCAGAT
     3541 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT
     3601 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC
     3661 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACCTG
     3721 AAAGCGAAAG GGAAACCAGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG
     3781 GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG
     3841 AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG
     3901 GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG
     3961 GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC
     4021 TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA
     4081 TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC
     4141 ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG
     4201 CAAGCGGCCA CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA
     4261 ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA
     4321 GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT
     4381 CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCCTCAATG ACGCTGACGG TACAGGCCAG
     4441 ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA TTGAGGCGCA
     4501 ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC
     4561 TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT
     4621 CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT
     4681 TGGAATCACA CGACCTGGAT GGAGTGGGAC AGAGAAATTA ACAATTACAC AAGCTTAATA
     4741 CACTCCTTAA TTGAAGAATC GCAAAACCAG CAAGAAAAGA ATGAACAAGA ATTATTGGAA
     4801 TTAGATAAAT GGGCAAGTTT GTGGAATTGG TTTAACATAA CAAATTGGCT GTGGTATATA
     4861 AAATTATTCA TAATGATAGT AGGAGGCTTG GTAGGTTTAA GAATAGTTTT TGCTGTACTT
     4921 TCTATAGTGA ATAGAGTTAG GCAGGGATAT TCACCATTAT CGTTTCAGAC CCACCTCCCA
     4981 ACCCCGAGGG GACCCGACAG GCCCGAAGGA ATAGAAGAAG AAGGTGGAGA GAGAGACAGA
     5041 GACAGATCCA TTCGATTAGT GAACGGATCT CGACGGTTAA CTTTTAAAAG AAAAGGGGGG
     5101 ATTGGGGGGT ACAGTGCAGG GGAAAGAATA GTAGACATAA TAGCAACAGA CATACAAACT
     5161 AAAGAATTAC AAAAACAAAT TACAAAAATT CAAAATTTTA TCGATACTAG TAAGGATCTG
     5221 CGATCGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG CCCACAGTCC CCGAGAAGTT
     5281 GGGGGGAGGG GTCGGCAATT GAACGGGTGC CTAGAGAAGG TGGCGCGGGG TAAACTGGGA
     5341 AAGTGATGTC GTGTACTGGC TCCGCCTTTT TCCCGAGGGT GGGGGAGAAC CGTATATAAG
     5401 TGCAGTAGTC GCCGTGAACG TTCTTTTTCG CAACGGGTTT GCCGCCAGAA CACAGCTGAA
     5461 GCTTCGAGGG GCTCGCATCT CTCCTTCACG CGCCCGCCGC CCTACCTGAG GCCGCCATCC
     5521 ACGCCGGTTG AGTCGCGTTC TGCCGCCTCC CGCCTGTGGT GCCTCCTGAA CTGCGTCCGC
     5581 CGTCTAGGTA AGTTTAAAGC TCAGGTCGAG ACCGGGCCTT TGTCCGGCGC TCCCTTGGAG
     5641 CCTACCTAGA CTCAGCCGGC TCTCCACGCT TTGCCTGACC CTGCTTGCTC AACTCTACGT
     5701 CTTTGTTTCG TTTTCTGTTC TGCGCCGTTA CAGATCCAAG CTGTGACCGG CGCCTACTCT
     5761 AGatgaacta ctttcccgat gaggtcattg agcacgtctt tgatttcgtc acaagccaca
     5821 aggataggaa cgccattagc ctggtctgta agagctggta caagatcgag cggtattcca
     5881 gacagaaggt gttcatcggc aactgctacg ccatcaatcc agagaggctg ctgcggagat
     5941 ttccctgtct gaagagcctg accctgaagg gcaagcccca cttcgccgac tttaacctgg
     6001 tgcctcacga gtggggagga ttcgtgctgc catggatcga ggccctggca cggtccagag
     6061 tgggcctgga ggagctgagg ctgaagcgca tggtggtgac agacgagtct ctggagctgc
     6121 tgtctcggag cttcgtgaac ttcaagagcc tggtgctggt gtcctgcgag ggctttacca
     6181 cagatggcct ggcaagcatc gcagcaaact gtaggcacct gcgggacctg gacctgcagg
     6241 agaatgagat cgacgatcac agaggccagt ggctgtcctg cttccccgat acctgtacca
     6301 cactggtgac actgaacttt gcctgcctgg agggcgagac aaatctggtg gccctggaga
     6361 ggctggtggc acgctctccc aacctgaaga gcctgaagct gaatagggca gtgcctctgg
     6421 acgcactggc aagactgatg gcatgcgcac cacagatcgt ggatctgggc gtgggctcct
     6481 acgagaacga ccctgattcc gagtcttatc tgaagctgat ggccgtgatc aagaagtgta
     6541 ccagcctgcg cagcctgtcc ggcttcctgg aggcagcacc tcactgcctg tccgcctttc
     6601 acccaatctg tcacaacctg acatccctga atctgtctta cgccgccgag atccacggct
     6661 cccacctgat caagctgatc cagcactgca agaagctgca gaggctgtgg atcctggact
     6721 ccatcggcga taagggcctg gaggtggtgg cctctacctg taaggagctg caggagctgc
     6781 gcgtgttccc atctgacctg ctgggaggag gaaacaccgc agtgacagag gagggcctgg
     6841 tggcaatctc tgccggatgc cccaagctgc acagcatcct gtatttttgt cagcagatga
     6901 ccaacgccgc cctggtgaca gtggccaaga actgccccaa tttcatcagg tttcgcctgt
     6961 gcatcctgga gcccaataag cctgaccacg tgacatctca gcctctggat gagggcttcg
     7021 gcgccatcgt gaaggcctgc aagagcctga ggcgcctgtc tctgagcggc ctgctgaccg
     7081 accaggtgtt cctgtacatc ggcatgtatg ccaaccagct ggagatgctg tctatcgcct
     7141 ttgccggcga cacagataag ggcatgctgt acgtgctgaa tggctgtaag aagatgaaga
     7201 agctggagat cagagacagc ccttttggcg ataccgccct gctggcagac gtgagcaagt
     7261 atgagacaat gcggagcctg tggatgagct cctgcgaggt gaccctgagc ggctgtaaga
     7321 gactggccga gaaggcccca tggctgaacg tggagatcat caacgagaat gacaacaata
     7381 ggatggagga gaatggccac gagggccgcc agaaggtgga taagctgtat ctgtatagga
     7441 ccgtcgtcgg gactcggatg gatgcccccc cctttgtctg gattctgggc ggatctggcg
     7501 ggatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag ctgtacatgg
     7561 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     7621 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     7681 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     7741 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     7801 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     7861 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     7921 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     7981 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga
     8041 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     8101 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     8161 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aatTCGACAA
     8221 TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC
     8281 TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT
     8341 GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG
     8401 GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG
     8461 TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT
     8521 TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT
     8581 GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC
     8641 CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA
     8701 TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG
     8761 CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GTACCTTTAA
     8821 GACCAATGAC TTACAAGGCA
//