LOCUS       pJET-07-mNeonGreen-R11a 4368 bp ds-DNA     circular     01-APR-2022
DEFINITION  .
KEYWORDS    "marker:AmpR"                                                      
FEATURES             Location/Qualifiers
     protein_bind    810..831
                     /label="CAP binding site"
     promoter        846..851
                     /label="lac UV5 promoter"
     promoter        846..876
                     /label="lac UV5 promoter"
     promoter        852..869
                     /label="lac UV5 promoter"
     promoter        870..876
                     /label="lac UV5 promoter"
     misc_feature    1311..1336
                     /label="T7 promoter preceded by 6 arbitrary nucleotides for optimal in vitro transcription"
     promoter        1317..1335
                     /label="T7 promoter"
     misc_feature    1336..1513
                     /label="intron"
     misc_feature    1336..1640
                     /label="5HDR"
     CDS             1641..2424
                     /label="Translation 1641-2424"
     misc_feature    1644..1664
                     /label="LinkerS1"
     misc_feature    1665..2369
                     /label="mNeonGreen"
     misc_feature    2370..2387
                     /label="linkerS2"
     misc_feature    2388..2393
                     /label="mut PAM"
     misc_feature    2388..2424
                     /label="Rab11exon1"
     misc_feature    2388..2704
                     /label="3HDR"
     primer_bind     complement(2752..2772)
                     /label="T7"
     promoter        3163..3267
                     /label="AmpR promoter"
     CDS             3268..3336
                     /label="AmpR"
     CDS             3337..4128
                     /label="AmpR"
     rep_origin      join(4299..4368,1..519)
                     /label="ori"
ORIGIN
        1 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
       61 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      541 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      601 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      661 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      721 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      781 aaagcaattg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      841 caggctttac actttatgct tccggctcgt ataatgtgtg gaattatgag cggataataa
      901 tttcacacag gaggtttaaa ctttaaacat gtcaaaagag acgtcttttg ttaagaatgc
      961 tgaggaactt gcaaagcaaa aaatggatgc tattaaccct gaactttctt caaaatttaa
     1021 atttttaata aaattcctgt ctcagtttcc tgaagcttgc tctaaacctc gttcaaaaaa
     1081 aatgcagaat aaagttggtc aagaggaaca tattgaatat ttagctcgta gttttcatga
     1141 gagtcgattg ccaagaaaac ccacgccacc tacaacggtt cctgatgagg tggttagcat
     1201 agttcttaat ataagtttta atatacagcc tgaaaatctt gagagaataa aagaagaaca
     1261 tcgattttcc atggcagctg agaatattgt aggagatctt ctagaaagat agctgataat
     1321 acgactcact atagggacta tggtagctag gagttccagg actcagtttc ccctttgagc
     1381 ctcctttagc gactaaagct tgaagcccca cgcatctcga ctctcgcgca caccgccctt
     1441 gttgggctca ggggcggggc gccgcccccg gaagtacttc cccttaaagg ctggggcctg
     1501 ccggaaatgg cgcagcggca gggaggggct cttcacccag tccggcagtt gaagctcggc
     1561 gctcgggtta cccctgcagc gacgccccct ggtcccacag ataccactgc tgctccgccc
     1621 tttcgctcct cggccgcgca atgggatcgg gtgggactag tggcgtgtct aagggcgaag
     1681 aggacaatat ggccagcctg cctgccacac acgagctgca catcttcggc agcatcaacg
     1741 gcgtggactt cgacatggtc ggacaaggca ccggcaatcc caacgacggc tacgaggaac
     1801 tgaacctgaa gtccaccaag ggcgacctgc agttcagccc ctggattctg gtgcctcaca
     1861 tcggctacgg cttccaccag tacctgcctt atcctgacgg catgagccct ttccaggccg
     1921 ccatggttga tggctctggc taccaggtgc accggaccat gcagtttgaa gatggcgcca
     1981 gcctgaccgt gaactaccgg tacacatacg agggcagcca catcaaaggc gaggcccaag
     2041 tgaaaggcac aggcttccct gctgatggcc ccgtgatgac caattctctg acagccgccg
     2101 actggtgcag aagcaagaaa acatacccca acgacaagac catcatcagc accttcaagt
     2161 ggtcctacac caccggcaac ggcaagcggt acagaagcac cgccagaacc acctacacct
     2221 tcgccaaacc tatggccgcc aactacctga agaaccagcc tatgtacgtg ttccgcaaga
     2281 ccgagctgaa gcactccaag acagaactga acttcaaaga gtggcagaaa gccttcaccg
     2341 acgtgatggg catggacgag ctgtacaagg gaaccggtgc tggaagtggt acacgcgacg
     2401 acgagtacga ctacctcttt aaaggtgagg ccatgggctc tcgcactcta cacagtcctc
     2461 gttcggggac ccgggccact cccggtggac cctcgtgccg gccacccctg cactgatata
     2521 ggcctccctc agcccttcct ttttgtgcgg ttccgtctcc tacccagctc agcctcttct
     2581 cccccgctca gacaggggtc cccatcacat gccgctctct gagcgacctc tccataggcc
     2641 ttcgctggcc tcagagcccc tccctgcgtg tccttcccct ggcggactgc cttctcccac
     2701 atcgatcttg ctgaaaaact cgagccatcc ggaagatctg gcggccgctc tccctatagt
     2761 gagtcgtatt acgccggatg gatatggtgt tcaggcacaa gtgttaaagc agttgatttt
     2821 attcactatg atgaaaaaaa caatgaatgg aacctgctcc aagttaaaaa tagagataat
     2881 accgaaaact catcgagtag taagattaga gataatacaa caataaaaaa atggtttaga
     2941 acttactcac agcgtgatgc tactaattgg gacaattttc cagatgaagt atcatctaag
     3001 aatttaaatg aagaagactt cagagctttt gttaaaaatt atttggcaaa aataatataa
     3061 ttcggctgca ggggcggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3121 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3181 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3241 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3301 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3361 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3421 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3481 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3541 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3601 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3661 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3721 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3781 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3841 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3901 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3961 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4021 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4081 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4141 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4201 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4261 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4321 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtt
//