LOCUS       pJET-62-SFFV-T2A-PuroR- 4880 bp ds-DNA     circular     01-APR-2022
DEFINITION  .
KEYWORDS    "marker:AmpR"                                                      
FEATURES             Location/Qualifiers
     CDS             15..68
                     /label="T2A"
     misc_feature    78..677
                     /label="Puromycin resistance"
     polyA_signal    715..939
                     /label="bGH poly(A) signal"
     misc_feature    946..966
                     /label="linkerS2"
     exon            967..1000
                     /label="RAB11B-001 Exon 1"
     misc_feature    967..1072
                     /label="3HDR"
     promoter        complement(1507..1513)
                     /label="lac UV5 promoter"
     promoter        complement(1514..1531)
                     /label="lac UV5 promoter"
     promoter        complement(1532..1537)
                     /label="lac UV5 promoter"
     protein_bind    1552..1573
                     /label="CAP binding site"
     rep_origin      complement(1864..2452)
                     /label="ori"
     CDS             complement(2623..3414)
                     /label="AmpR"
     CDS             complement(3415..3483)
                     /label="AmpR"
     promoter        complement(3484..3588)
                     /label="AmpR promoter"
     promoter        3980..3998
                     /label="T7 promoter"
     promoter        4048..4066
                     /label="T7 promoter"
     misc_feature    4067..4368
                     /label="5HDR"
     promoter        4389..4796
                     /label="SFFV promoter"
     CDS             join(4871..4880,1..677)
                     /label="Translation 4871-677"
ORIGIN
        1 ctagtggcag cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
       61 ccggccctag gctcgagatg acggaatata agcccacagt cagattggct acgagagatg
      121 atgttccgcg agccgtgcgg acgctcgcag ccgcttttgc agactatcct gctacgcgac
      181 atactgttga ccctgatagg catatcgaaa gagtaaccga acttcaggag ctctttctga
      241 ctagggtcgg tttggatatt gggaaggtct gggtagccga cgacggcgcc gctgtcgccg
      301 tatggacgac tccagagagc gtagaagcgg gagcggtatt tgctgagatc ggtccacgga
      361 tggcagaatt gtccggcagt agacttgcgg ctcaacagca aatggaagga cttctcgcac
      421 cgcatcgacc caaagagccc gcctggtttc ttgctacggt aggggtgtcc cccgaccacc
      481 agggtaaggg tctcggaagt gctgttgtcc ttccaggagt ggaagcggca gagcgcgcgg
      541 gtgttcctgc gtttctggag acctcagccc caaggaacct tcctttttat gaacgcttgg
      601 gatttactgt cacggccgat gttgaggttc ctgaaggacc tcggacatgg tgcatgacaa
      661 ggaaaccagg ggcgtgatct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
      721 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
      781 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
      841 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
      901 acaatagcag gcatgctggg gatgcggtgg gctctatggg tcgacggaac cggtgctgga
      961 agtggtaccc gggacgacga gtacgactac ctattcaaag gtgcggccgg tggggcacag
     1021 acgggcgaag tcgtggcggc gaggcggcgg gcagacgggc caaggccgcg ctatctttct
     1081 agaagatctc ctacaatatt ctcagctgcc atggaaaatc gatgttcttc ttttattctc
     1141 tcaagatttt caggctgtat attaaaactt atattaagaa ctatgctaac cacctcatca
     1201 ggaaccgttg taggtggcgt gggttttctt ggcaatcgac tctcatgaaa actacgagct
     1261 aaatattcaa tatgttcctc ttgaccaact ttattctgca ttttttttga acgaggttta
     1321 gagcaagctt caggaaactg agacaggaat tttattaaaa atttaaattt tgaagaaagt
     1381 tcagggttaa tagcatccat tttttgcttt gcaagttcct cagcattctt aacaaaagac
     1441 gtctcttttg acatgtttaa agtttaaacc tcctgtgtga aattattatc cgctcataat
     1501 tccacacatt atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1561 ctaactcaca ttaattgcgt tgcgctcact gccaattgct ttccagtcgg gaaacctgtc
     1621 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1681 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1741 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1801 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1861 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1921 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1981 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2041 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2101 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2161 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2221 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2281 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2341 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2401 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2461 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2521 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2581 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2641 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2701 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2761 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2821 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2881 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2941 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3001 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3061 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3121 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3181 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3241 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3301 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3361 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3421 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3481 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3541 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3601 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3661 gcgtatcacg aggccgcccc tgcagccgaa ttatattatt tttgccaaat aatttttaac
     3721 aaaagctctg aagtcttctt catttaaatt cttagatgat acttcatctg gaaaattgtc
     3781 ccaattagta gcatcacgct gtgagtaagt tctaaaccat ttttttattg ttgtattatc
     3841 tctaatctta ctactcgatg agttttcggt attatctcta tttttaactt ggagcaggtt
     3901 ccattcattg tttttttcat catagtgaat aaaatcaact gctttaacac ttgtgcctga
     3961 acaccatatc catccggcgt aatacgactc actataggga gagcggccgc cagatcttcc
     4021 ggatggctcg agtttttcag caagatataa tacgactcac tatagggcag tcttccgccc
     4081 cgccctcttg agctcgcccc tgataggctg gcgcgtccgt cacttcaaaa aggtccgcat
     4141 tccttccgcc tttctccagg acaccgaggg cgaggagggt ggtaccaagc ggcgcccacc
     4201 ctcagagcac tacttccatc tctgattggc ttcgctgggt gcccgtcgct actccactcg
     4261 ccgctgatag gccattcaac cgccgctctg cgcggcgccg gctccgcccc cgtcgggtgt
     4321 ttgtggtggg gctgcggagt cgccgatccc gccggaagcg ccaggacaat gggatcgggt
     4381 gggactaggt aacgccattt tgcaaggcat ggaaaaatac caaaccaaga atagagaagt
     4441 tcagatcaag ggcgggtaca tgaaaatagc taacgttggg ccaaacagga tatctgcggt
     4501 gagcagtttc ggccccggcc cggggccaag aacagatggt caccgcagtt tcggccccgg
     4561 cccgaggcca agaacagatg gtccccagat atggcccaac cctcagcagt ttcttaagac
     4621 ccatcagatg tttccaggct cccccaagga cctgaaatga ccctgcgcct tatttgaatt
     4681 aaccaatcag cctgcttctc gcttctgttc gcgcgcttct gcttcccgag ctctataaaa
     4741 gagctcacaa cccctcactc ggcgcgccag tcctccgaca gactgagtcg cccggggggg
     4801 atctggagct ctcgagaatt ctcacgcgtc tgcaggatat caagcttgcg gtaccgcggg
     4861 cccgggatcc atggtgagca
//