LOCUS       pJET_63-SFFV-T2A-BlaR-p 4897 bp ds-DNA     circular     01-APR-2022
DEFINITION  .
KEYWORDS    "3' Cloning site - Enzyme Used" "3' primer" "5' Cloning site -     
            Enzyme Used" "5' primer" "Backbone" "Created by" "Gene or insert"
            "Location" "Location details"
            "Name:pJET_63-SFFV-T2A-BlaR-polyA-Rab11A" "Owner"
            "Plasmid:pJET_63-SFFV-T2A-BlaR-polyA-Rab11A_NGS correct"
            "Resistance (bacteria)" "Strain/Frozen stock" "Sub-location"
            "Technical details"
FEATURES             Location/Qualifiers
     protein_bind    165..186
                     /label="CAP binding site"
     promoter        201..231
                     /label="lac UV5 promoter"
     misc_feature    666..691
                     /label="T7 promoter preceded by 6 arbitrary nucleotides for optimal in vitro transcription"
     promoter        672..690
                     /label="T7 promoter"
     misc_feature    691..868
                     /label="intron"
     misc_feature    691..996
                     /label="5HDR"
     promoter        1017..1424
                     /label="SFFV promoter"
     misc_feature    1499..1507
                     /label="START"
     exon            1499..1984
                     /label="Translation 1499-1984"
     CDS             1523..1576
                     /label="T2A"
     CDS             1586..1984
                     /label="BSD"
     polyA_signal    2022..2246
                     /label="bGH poly(A) signal"
     misc_feature    2271..2307
                     /label="Rab11exon1"
     misc_feature    2271..2588
                     /label="3HDR"
     promoter        complement(2637..2655)
                     /label="T7 promoter"
     promoter        3047..3151
                     /label="AmpR promoter"
     CDS             3152..3220
                     /label="AmpR"
     exon            3152..3220
                     /label="Translation 3152-3220"
     CDS             3221..4012
                     /label="AmpR"
     exon            3221..4012
                     /label="Translation 3221-4012"
     rep_origin      4183..4771
                     /label="ori"
ORIGIN
        1 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
       61 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      121 aggtttcccg actggaaagc aattggcagt gagcgcaacg caattaatgt gagttagctc
      181 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggaatt
      241 atgagcggat aataatttca cacaggaggt ttaaacttta aacatgtcaa aagagacgtc
      301 ttttgttaag aatgctgagg aacttgcaaa gcaaaaaatg gatgctatta accctgaact
      361 ttcttcaaaa tttaaatttt taataaaatt cctgtctcag tttcctgaag cttgctctaa
      421 acctcgttca aaaaaaatgc agaataaagt tggtcaagag gaacatattg aatatttagc
      481 tcgtagtttt catgagagtc gattgccaag aaaacccacg ccacctacaa cggttcctga
      541 tgaggtggtt agcatagttc ttaatataag ttttaatata cagcctgaaa atcttgagag
      601 aataaaagaa gaacatcgat tttccatggc agctgagaat attgtaggag atcttctaga
      661 aagatagctg ataatacgac tcactatagg gactatggta gctaggagtt ccaggactca
      721 gtttcccctt tgagcctcct ttagcgacta aagcttgaag ccccacgcat ctcgactctc
      781 gcgcacaccg cccttgttgg gctcaggggc ggggcgccgc ccccggaagt acttcccctt
      841 aaaggctggg gcctgccgga aatggcgcag cggcagggag gggctcttca cccagtccgg
      901 cagttgaagc tcggcgctcg ggttacccct gcagcgacgc cccctggtcc cacagatacc
      961 actgctgctc ccgccctttc gctcctcggc cgcgcaATGG GATCGGGTGG GACTAGGTAA
     1021 CGCCATTTTG CAAGGCATGG AAAAATACCA AACCAAGAAT AGAGAAGTTC AGATCAAGGG
     1081 CGGGTACATG AAAATAGCTA ACGTTGGGCC AAACAGGATA TCTGCGGTGA GCAGTTTCGG
     1141 CCCCGGCCCG GGGCCAAGAA CAGATGGTCA CCGCAGTTTC GGCCCCGGCC CGAGGCCAAG
     1201 AACAGATGGT CCCCAGATAT GGCCCAACCC TCAGCAGTTT CTTAAGACCC ATCAGATGTT
     1261 TCCAGGCTCC CCCAAGGACC TGAAATGACC CTGCGCCTTA TTTGAATTAA CCAATCAGCC
     1321 TGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TTCCCGAGCT CTATAAAAGA GCTCACAACC
     1381 CCTCACTCGG CGCGCCAGTC CTCCGACAGA CTGAGTCGCC CGGGGGGGAT CTGGAGCTCT
     1441 CGAGAATTCT CACGCGTCTG CAGGATATCA AGCTTGCGGT ACCGCGGGCC CGGGATCCAT
     1501 GGTGAGCACT AGTGGCAGCG GAGAGGGCAG AGGAAGTCTT CTAACATGCG GTGACGTGGA
     1561 GGAGAATCCC GGCCCTAGGC TCGAGatggc caagcctttg tctcaagaag aatccaccct
     1621 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     1681 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     1741 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     1801 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     1861 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     1921 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     1981 ctaaTCtaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     2041 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     2101 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     2161 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     2221 tgctggggat gcggtgggct ctatgggtcg acggaaccgg tgctggaagt ggtacacgcg
     2281 acgacgagta cgactacctc tttaaaggtg aggccatggg ctctcgcact ctacacagtc
     2341 ctcgttcggg gacccgggcc actcccggtg gaccctcgtg ccggccaccc ctgcactgat
     2401 ataggcctcc ctcagccctt cctttttgtg cggttccgtc tcctacccag ctcagcctct
     2461 tctcccccgc tcagacaggg gtccccatca catgccgctc tctgagcgac ctctccatag
     2521 gccttcgctg gcctcagagc ccctccctgc gtgtccttcc cctggcggac tgccttctcc
     2581 cacatcgtat cttgctgaaa aactcgagcc atccggaaga tctggcggcc gctctcccta
     2641 tagtgagtcg tattacgccg gatggatatg gtgttcaggc acaagtgtta aagcagttga
     2701 ttttattcac tatgatgaaa aaaacaatga atggaacctg ctccaagtta aaaatagaga
     2761 taataccgaa aactcatcga gtagtaagat tagagataat acaacaataa aaaaatggtt
     2821 tagaacttac tcacagcgtg atgctactaa ttgggacaat tttccagatg aagtatcatc
     2881 taagaattta aatgaagaag acttcagagc ttttgttaaa aattatttgg caaaaataat
     2941 ataattcggc tgcaggggcg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3001 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3061 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3121 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3181 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3241 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3301 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3361 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3421 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3481 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3541 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3601 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3661 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3721 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3781 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3841 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3901 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3961 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4021 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4081 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4141 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4201 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4261 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4321 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4381 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4441 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4501 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4561 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4621 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4681 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4741 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4801 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4861 ggataaccgt attaccgcct ttgagtgagc tgatacc
//