LOCUS       pJET-59-DExCon-mTagBFP2 5044 bp ds-DNA     circular     01-APR-2022
DEFINITION  .
KEYWORDS    "marker:AmpR"                                                      
FEATURES             Location/Qualifiers
     protein_bind    7..25
                     /label="tet operator"
     protein_bind    43..61
                     /label="tet operator"
     protein_bind    79..97
                     /label="tet operator"
     protein_bind    115..133
                     /label="tet operator"
     CDS             268..1060
                     /label="Translation 268-1060"
     CDS             292..1002
                     /label="mTagBFP2"
     exon            1004..1060
                     /label="RAB25-001 Exon 1b"
     misc_feature    1004..1379
                     /label="3HDR"
     promoter        complement(1489..1507)
                     /label="T7 promoter"
     promoter        1899..2003
                     /label="AmpR promoter"
     CDS             2004..2072
                     /label="AmpR"
     CDS             2073..2864
                     /label="AmpR"
     rep_origin      3035..3623
                     /label="ori"
     protein_bind    3914..3935
                     /label="CAP binding site"
     promoter        3950..3955
                     /label="lac UV5 promoter"
     promoter        3956..3973
                     /label="lac UV5 promoter"
     promoter        3974..3980
                     /label="lac UV5 promoter"
     primer_bind     4485..4501
                     /label="M13 fwd"
     promoter        4512..4530
                     /label="T7 promoter"
     CDS             4535..4546
                     /label="Factor Xa site"
     promoter        4568..4586
                     /label="T7 promoter"
     misc_feature    4585..4690
                     /label="intron"
     misc_feature    4585..4931
                     /label="5HDR"
     misc_feature    4691..4931
                     /label="Rab25 exon1a"
     misc_feature    join(4938..5044,1..258)
                     /label="TRE3GS promoter"
     protein_bind    4943..4961
                     /label="tet operator"
     protein_bind    4979..4997
                     /label="tet operator"
     protein_bind    5015..5033
                     /label="tet operator"
ORIGIN
        1 gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag
       61 aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta
      121 tcagtgatag agaacgtata agctttgctt atgtaaacca gggcgcctat aaaagagtgc
      181 tgattttttg agtaaacttc aattccacaa cacttttgtc ttataccaac tttccgtacc
      241 acttcctacc ctcgtaaaga attcaagatg gttggatcgg gtgggactag tatggtgtct
      301 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
      361 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
      421 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
      481 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
      541 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
      601 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
      661 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
      721 tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
      781 atggccctga agctcgtggg cgggagccat ctgatcgcaa acgccaagac cacatataga
      841 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
      901 gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc
      961 agatactgcg acctccctag caaactgggg cacaagctta ataccggtgc tggaagtggt
     1021 gggaatggaa ctgaggaaga ttataacttt gtcttcaagg gtgagttggg ttccctgaag
     1081 caagaggaag cctgagagac ccagaaagag attgaataga gccagagagg gccccctcct
     1141 gtttttttat ctcttaccaa gacctcctgg ggctcctcat tccttcagaa agaaagctgc
     1201 agggagtggg ggagggtctg gttgcagggg caaaagtcag ggagcttctg agtatgctct
     1261 actgtggtaa ataatgatgt tgattcctcc ttaagagtgc tgagttaggc tcctctgccc
     1321 cgttcctaat agaaacccaa atccctggtg tcagccagtg cagcacaaga gatggaggtc
     1381 tgggcctcat gggccttcct ttcactgccc gctttccagt cgggaaacct gtcgtgccag
     1441 atcttgctga aaaactcgag ccatccggaa gatctggcgg ccgctctccc tatagtgagt
     1501 cgtattacgc cggatggata tggtgttcag gcacaagtgt taaagcagtt gattttattc
     1561 actatgatga aaaaaacaat gaatggaacc tgctccaagt taaaaataga gataataccg
     1621 aaaactcatc gagtagtaag attagagata atacaacaat aaaaaaatgg tttagaactt
     1681 actcacagcg tgatgctact aattgggaca attttccaga tgaagtatca tctaagaatt
     1741 taaatgaaga agacttcaga gcttttgtta aaaattattt ggcaaaaata atataattcg
     1801 gctgcagggg cggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     1861 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1921 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1981 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2041 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2101 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2161 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2221 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2281 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2341 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2401 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2461 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2521 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2581 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2641 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     2701 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     2761 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     2821 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2881 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2941 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3001 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3061 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3781 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3841 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagca attggcagtg
     3901 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     3961 tgcttccggc tcgtataatg tgtggaatta tgagcggata ataatttcac acaggaggtt
     4021 taaactttaa acatgtcaaa agagacgtct tttgttaaga atgctgagga acttgcaaag
     4081 caaaaaatgg atgctattaa ccctgaactt tcttcaaaat ttaaattttt aataaaattc
     4141 ctgtctcagt ttcctgaagc ttgctctaaa cctcgttcaa aaaaaatgca gaataaagtt
     4201 ggtcaagagg aacatattga atatttagct cgtagttttc atgagagtcg attgccaaga
     4261 aaacccacgc cacctacaac ggttcctgat gaggtggtta gcatagttct taatataagt
     4321 tttaatatac agcctgaaaa tcttgagaga ataaaagaag aacatcgatt ttccatggca
     4381 gctgagaata ttgtaggaga tcttctagaa agatctggcg aaagggggat gtgctgcaag
     4441 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     4501 tgagcgcgac gtaatacgac tcactatagg gcgaattgaa ggaaggccgt caaggccgca
     4561 tagctgataa tacgactcac tatagggagc ccaggctgag agcagacacc caacctgtcg
     4621 aacctgtctg acgtcatcat ctctccaccc acctgggccc caggtctcca gccaccccgc
     4681 tcttcctgtt ctcagcttcc gtcctctctg cttccttaca gcacccccac ctgccagagc
     4741 tgatcctccc taggccctgc ctaaccttga gttggccccc aatccctctg gctgcagaag
     4801 tccccttacc cccaatgaga ggaggggcag gaccagatct tttgagagct gagggttgag
     4861 ggcattgagc caacacacag atttgtcgcc tctgtccccg aagacacctg caccctccat
     4921 gcggagccaa ggaattcttt actccctatc agtgatagag aacgtatgaa gagtttactc
     4981 cctatcagtg atagagaacg tatgcagact ttactcccta tcagtgatag agaacgtata
     5041 agga
//