LOCUS       Exported                6117 bp DNA     circular SYN 10-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 10, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1622
                     /locus_tag="CAG"
                     /label=CAG
                     /note="color: #ff4922"
     misc_feature    1725..1734
                     /locus_tag="MCS"
                     /label=MCS
                     /note="color: #ffff0f"
     CDS             1747..3054
                     /codon_start=1
                     /label=CBX5-F44E-eGFP
                     /note="color: #ccffcc"
                     /translation="MGKKTKRTADSSSSEDEEEYVVEKVLDRRMVKGQVEYLLKWKGES
                     EEHNTWEPEKNLDCPELISEFMKKYKKMKEGENNKPREKSEGNKRKSSFSNSADDIKSK
                     KKREQSNDIARGFERGLEPEKIIGATDSCGDLMFLMKWKDTDEADLVLAKEANVKCPQI
                     VIAFYEERLTWHAYPEDAENKEKESAKSLPVATMVSKGEELFTGVVPILVELDGDVNGH
                     KFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFF
                     KSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEY
                     NYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLS
                     TQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1747..1749
                     /locus_tag="START"
                     /label=START
                     /note="color: #31ff25"
     misc_feature    1795..1959
                     /locus_tag="Chromobox"
                     /label=Chromobox
                     /note="color: #ecff4b"
     misc_feature    1876..1878
                     /label=F44E
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    1960..2091
                     /locus_tag="HINGE"
                     /label=HINGE
                     /note="color: #74ff4e"
     misc_feature    2092..2274
                     /locus_tag="CSD"
                     /label=CSD
                     /note="color: #ff5ce8"
     misc_feature    2335..3054
                     /locus_tag="EGFP"
                     /label=EGFP
                     /note="color: #02ff1b"
     misc_feature    complement(5126..5986)
                     /locus_tag="Amp"
                     /label=Amp
                     /note="color: #1f28ff; direction: LEFT"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 cacgacatgg gaaagaagac caagaggaca gccgacagct cttcttcaga ggatgaggag
     1801 gaatatgtgg tggaaaaggt gttggacagg cgcatggtta aggggcaagt ggaatatctg
     1861 ttgaagtgga aaggcgagtc tgaggagcac aatacttggg aacctgagaa gaacttggat
     1921 tgtcctgaac taatttctga gtttatgaaa aagtataaga agatgaagga gggtgaaaac
     1981 aataagccca gggagaaatc agaaggaaac aagaggaaat ccagtttctc caacagcgct
     2041 gatgatatta aatctaaaaa aaagagagag caaagcaatg atatcgctcg gggctttgag
     2101 agaggactgg aaccagaaaa gatcatcgga gcaacagatt cctgcggtga cttaatgttc
     2161 ttaatgaaat ggaaagacac agatgaagct gacctggttc ttgcaaaaga agctaacgtg
     2221 aagtgtccac agattgtgat agcattttat gaagagagac tgacgtggca cgcatatcca
     2281 gaggatgcgg aaaacaaaga aaaagaaagc gcgaagagct taccggtcgc caccatggtg
     2341 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2401 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     2461 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2521 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     2581 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     2641 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2701 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2761 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     2821 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     2881 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2941 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3001 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     3061 cgcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     3121 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     3181 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     3241 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     3301 cagaatgagt atttggttta gagtttggca acatatgcca tatgctggct gccatgaaca
     3361 aaggtggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     3421 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     3481 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     3541 ctgactactc ccagtcatag ctgtccctct tctcttatga agatccctcg acctgcagcc
     3601 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3661 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3721 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3781 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3961 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt
     4021 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4081 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat
     4141 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4201 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4261 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4321 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4381 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4441 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4501 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4561 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4621 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4681 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4741 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4801 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4861 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4921 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4981 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5041 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5101 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5161 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5221 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5281 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5341 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5401 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5461 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5521 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5581 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5641 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5701 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5761 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5821 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5881 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5941 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6001 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6061 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
