LOCUS       Exported                5396 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..507
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    596..636
                     /locus_tag="HSV TK 5'-UTR "
                     /label=HSV TK 5'-UTR
                     
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             668..1045
                     /codon_start=1
                     /label=IFITM1
                     /translation="MHKEEHEVAVLGAPPSTILPRSTVINIHSETSVPDHVVWSLFNTL
                     FLNWCCLGFIAFAYSVKSRDRKMVGDVTGAQAYASTAKCLNIWALILGILMTIGFILLL
                     VFGSVTVYHIMLQIIQEKRGY"
     intron          1075..1647
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    1844..1899
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    2236..2432
                     /locus_tag="SV40 early promoter"
                     /label=SV40 early promoter
     promoter        complement(2236..2432)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2250..2385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2670..3258)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3432..4091
                     /locus_tag="AmpR"
                     /label=AmpR
     CDS             complement(3727..4105)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(4300..4404)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4736..5191)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5336..5352
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5359..5377
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(5396,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagaatatg cacaaggagg aacatgaggt ggctgtgctg ggggcacccc ccagcaccat
      721 ccttccaagg tccaccgtga tcaacatcca cagcgagacc tccgtgcccg accatgtcgt
      781 ctggtccctg ttcaacaccc tcttcttgaa ctggtgctgt ctgggcttca tagcattcgc
      841 ctactccgtg aagtctaggg acaggaagat ggttggcgac gtgaccgggg cccaggccta
      901 tgcctccacc gccaagtgcc tgaacatctg ggccctgatt ctgggcatcc tcatgaccat
      961 tggattcatc ctgttactgg tattcggctc tgtgacagtc taccatatta tgttacagat
     1021 aatacaggaa aaacggggtt actagtagac gcgtcggatc ctgagaactt cagggtgagt
     1081 ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg
     1141 ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc
     1201 tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt
     1261 ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt
     1321 aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact tttttttcaa
     1381 ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt
     1441 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt
     1501 ggtagaaaca actacatcct ggtcatcatc ctgcctttct ctttatggtt acaatgatat
     1561 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat
     1621 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttgt tgtgctgtct
     1681 catcattttg gcaaagaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
     1741 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
     1801 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     1861 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     1921 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgccata
     1981 tgctggctgc catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc
     2041 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     2101 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     2161 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag
     2221 atcctcgaga agctgtttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     2281 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     2341 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     2401 cgggactatg gttgctgact aattgagatg cagctgcatt aatgaatcgg ccaacgcgcg
     2461 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     2521 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2581 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2641 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2701 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2761 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2821 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2881 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2941 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3001 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3061 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     3121 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3181 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3241 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3301 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3361 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3421 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3481 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3541 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3601 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3661 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3721 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3781 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3841 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3901 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3961 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4021 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4081 aaagtgctca tcattggaaa acgttcgaaa acgttcttcg gggcgaaaac tctcaaggat
     4141 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4201 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4261 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4321 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4381 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     4441 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     4501 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     4561 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4621 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     4681 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     4741 gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     4801 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     4861 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     4921 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     4981 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     5041 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     5101 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     5161 gccgcgctta atgcgccgct acagggcgcg tcgcgccatt cgccattcag gctgcgcaac
     5221 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     5281 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     5341 acgacggcca gtgaattgta atacgactca ctatagggcg aattcgagct cgcccc
//
