LOCUS       Exported                6722 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..507
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    596..636
                     /locus_tag="HSV TK 5'-UTR "
                     /label=HSV TK 5'-UTR
                     
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             668..1045
                     /codon_start=1
                     /label=IFITM1
                     /translation="MHKEEHEVAVLGAPPSTILPRSTVINIHSETSVPDHVVWSLFNTL
                     FLNWCCLGFIAFAYSVKSRDRKMVGDVTGAQAYASTAKCLNIWALILGILMTIGFILLL
                     VFGSVTVYHIMLQIIQEKRGY"
     CDS             1108..1659
                     /codon_start=1
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /translation="TLLAEAAWNKAGVRLSICYFPPYCRLLAM*GPGNLALSS*RAFLG
                     VFPLSPKECKVC*MS*RKQFLWKLLEDKQRL*RPFAGSGTPHLATGASAAKSHVYKIHL
                     QRRHNPSATL*VG*LWKESNGSPQAYSTRG*RMPRRYPIVWDLIWGLGAHALHVFSRG*
                     KTSRPPEPRGRGFPLKNTMII"
     CDS             1662..2381
                     /codon_start=1
                     /label=eGFP
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYN"
     intron          2401..2973
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    3170..3225
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    3562..3758
                     /locus_tag="SV40 early promoter"
                     /label=SV40 early promoter
     promoter        complement(3562..3758)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3576..3711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(3996..4584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4758..5417
                     /locus_tag="AmpR"
                     /label=AmpR
     CDS             complement(5053..5431)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(5626..5730)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6062..6517)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     6662..6678
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6685..6703
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(6722,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagaatatg cacaaggagg aacatgaggt ggctgtgctg ggggcacccc ccagcaccat
      721 ccttccaagg tccaccgtga tcaacatcca cagcgagacc tccgtgcccg accatgtcgt
      781 ctggtccctg ttcaacaccc tcttcttgaa ctggtgctgt ctgggcttca tagcattcgc
      841 ctactccgtg aagtctaggg acaggaagat ggttggcgac gtgaccgggg cccaggccta
      901 tgcctccacc gccaagtgcc tgaacatctg ggccctgatt ctgggcatcc tcatgaccat
      961 tggattcatc ctgttactgg tattcggctc tgtgacagtc taccatatta tgttacagat
     1021 aatacaggaa aaacggggtt actagtagac gcgtcggatc ccggagtact acaaagactg
     1081 ctgacgcgaa ttccgccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     1141 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     1201 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     1261 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     1321 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     1381 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     1441 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     1501 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     1561 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca
     1621 cggggacgtg gttttccttt gaaaaacacg atgataatac catggctagc aaaggagaag
     1681 aactcttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aacggccaca
     1741 agttctctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt accctgaagt
     1801 tcatctgcac tactggcaaa ctgcctgttc catggccaac actagtcact actctgtgct
     1861 atggtgttca atgcttttca agatacccgg atcatatgaa acggcatgac tttttcaaga
     1921 gtgccatgcc cgaaggttat gtacaggaaa ggaccatctt cttcaaagat gacggcaact
     1981 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
     2041 aaggtattga cttcaaggaa gatggcaaca ttctgggaca caaattggaa tacaactata
     2101 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gtgaacttca
     2161 agacccgcca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
     2221 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     2281 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
     2341 ctgctgggat tacacatggc atggatgaac tgtacaactg aggatcctga gaacttcagg
     2401 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat
     2461 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
     2521 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
     2581 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga
     2641 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt
     2701 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt
     2761 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt
     2821 cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt atggttacaa
     2881 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct
     2941 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttgttgtg
     3001 ctgtctcatc attttggcaa agaattcact cctcaggtgc aggctgccta tcagaaggtg
     3061 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
     3121 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     3181 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     3241 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     3301 gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt atatgaaaca
     3361 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     3421 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     3481 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     3541 atgaagatcc tcgagaagct gtttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     3601 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     3661 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     3721 taggggcggg actatggttg ctgactaatt gagatgcagc tgcattaatg aatcggccaa
     3781 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     3841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3901 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4201 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4321 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4381 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     4441 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4501 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4561 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4621 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4681 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4741 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4801 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4861 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4921 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4981 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5041 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5101 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5161 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5221 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5281 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5341 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5401 actttaaaag tgctcatcat tggaaaacgt tcgaaaacgt tcttcggggc gaaaactctc
     5461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5641 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5701 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     5761 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     5821 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     5881 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     5941 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     6001 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     6061 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     6121 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     6181 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     6241 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     6301 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     6361 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     6421 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     6481 acacccgccg cgcttaatgc gccgctacag ggcgcgtcgc gccattcgcc attcaggctg
     6541 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     6601 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     6661 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt cgagctcgcc
     6721 cc
//
