LOCUS       Exported                6743 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..507
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    596..636
                     /locus_tag="HSV TK 5'-UTR "
                     /label=HSV TK 5'-UTR
                     
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             668..1068
                     /codon_start=1
                     /label=IFITM3
                     /translation="MNHTVQTFFSPVNSGQPPNYEMLKEEHEVAVLGAPHNPAPPTSTV
                     IHIRSETSVPDHVVWSLFNTLFMNPCCLGFIAFAYSVKSRDRKMVGDVTGAQAYASTAK
                     CLNIWALILGILMTILLIVIPVLIFQAYG"
     CDS             1129..1680
                     /codon_start=1
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /translation="TLLAEAAWNKAGVRLSICYFPPYCRLLAM*GPGNLALSS*RAFLG
                     VFPLSPKECKVC*MS*RKQFLWKLLEDKQRL*RPFAGSGTPHLATGASAAKSHVYKIHL
                     QRRHNPSATL*VG*LWKESNGSPQAYSTRG*RMPRRYPIVWDLIWGLGAHALHVFSRG*
                     KTSRPPEPRGRGFPLKNTMII"
     CDS             1683..2402
                     /codon_start=1
                     /product="photostable monomeric blue variant of GFP (Ai et 
                     al., 2007)"
                     /label=BFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVEMDGDVNGRKFSVRGVGEGDATHGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GSYKTRAEVKFEGDTLVNRIVLKGTDFKEDGNILGHKLEYNMNVGNVYITADKQKNGIK
                     ANFEIRHNVEDGGVQLADHYQQNTPIGDGSVLLPDNHYLSVQVKLSKDPNEKRDHMVLL
                     EFRTAAGITPGMDELYK"
     intron          2422..2994
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    3191..3246
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    3583..3779
                     /locus_tag="SV40 early promoter"
                     /label=SV40 early promoter
     promoter        complement(3583..3779)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3597..3732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(4017..4605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4779..5438
                     /locus_tag="AmpR"
                     /label=AmpR
     CDS             complement(5074..5452)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(5647..5751)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6083..6538)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     6683..6699
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6706..6724
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(6743,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagaccatg aatcacactg tccaaacctt cttctctcct gtcaacagtg gccagccccc
      721 caactatgag atgctcaagg aggagcacga ggtggctgtg ctgggggcgc cccacaaccc
      781 tgctcccccg acgtccaccg tgatccacat ccgcagcgag acctccgtgc ccgaccatgt
      841 cgtctggtcc ctgttcaaca ccctcttcat gaacccctgc tgcctgggct tcatagcatt
      901 cgcctactcc gtgaagtcta gggacaggaa gatggttggc gacgtgaccg gggcccaggc
      961 ctatgcctcc accgccaagt gcctgaacat ctgggccctg attctgggca tcctcatgac
     1021 cattctgctc atcgtcatcc cagtgctgat cttccaggcc tatggataga cgcgtcggat
     1081 cccggagtac tacaaagact gctgacgcga attccgcccc cccccctaac gttactggcc
     1141 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     1201 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     1261 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     1321 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     1381 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     1441 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     1501 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     1561 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     1621 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     1681 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagatgg
     1741 acggcgacgt aaacggccgc aagttcagcg tgcgcggcgt gggcgagggt gatgccaccc
     1801 acggcaagct gaccctgaag ttcatctgca cctccggcaa gctgcccgtg ccctggccta
     1861 ccctcgtgac caccctgtct tacggcgtgc agtgcttctc ccgctacccc gaccacatga
     1921 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     1981 tcttcaagga cgacggcagc tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2041 tggtgaaccg catcgtgctg aagggcaccg acttcaagga ggacggtaac atcctggggc
     2101 acaagctgga gtacaacatg aacgtcggga acgtctatat cacggccgac aagcagaaga
     2161 acggcatcaa agcgaacttc gagatcaggc acaacgtcga ggacggcggc gtgcagctcg
     2221 ccgaccacta ccagcagaac acccccatcg gcgacggctc cgtgctgctg cccgacaacc
     2281 actacctgag cgtgcaggtg aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     2341 tcctgctgga gttccgcacc gccgccggga tcactcccgg catggacgag ctgtacaagt
     2401 aaggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct
     2461 attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga
     2521 agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc
     2581 tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac
     2641 tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag
     2701 tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca
     2761 cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat
     2821 tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt catcatcctg
     2881 cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag
     2941 tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     3001 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcac tcctcaggtg
     3061 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac
     3121 tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     3181 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     3241 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     3301 gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt ggctataaag
     3361 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     3421 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     3481 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     3541 catagctgtc cctcttctct tatgaagatc ctcgagaagc tgtttgcaaa agcctaggcc
     3601 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     3661 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     3721 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcag
     3781 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3841 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3901 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3961 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4021 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4081 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4141 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4201 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4261 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4321 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4381 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4441 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4501 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4561 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4621 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4681 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4741 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4801 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4861 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4921 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4981 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5041 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5101 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5161 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5221 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5281 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5341 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5401 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcgaaaacg
     5461 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5521 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5581 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5641 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5701 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5761 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     5821 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     5881 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     5941 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     6001 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     6061 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
     6121 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     6181 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     6241 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     6301 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     6361 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     6421 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     6481 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg
     6541 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     6601 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     6661 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     6721 tagggcgaat tcgagctcgc ccc
//
