LOCUS       Exported               10833 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    CoV1 NSP3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             666..6485
                     /codon_start=1
                     /label=CoV1 NSP3
                     /translation="ATMAPIKGVTFGEDTVWEVQGYKNVRITFELDERVDKVLNEKCSV
                     YTVESGTEVTEFACVVAEAVVKTLQPVSDLLTNMGIDLDEWSVATFYLFDDAGEENFSS
                     RMYCSFYPPDEEEEDDAECEEEEIDETCEHEYGTEDDYQGLPLEFGASAETVRVEEEEE
                     EDWLDDTTEQSEIEPEPEPTPEEPVNQFTGYLKLTDNVAIKCVDIVKEAQSANPMVIVN
                     AANIHLKHGGGVAGALNKATNGAMQKESDDYIKLNGPLTVGGSCLLSGHNLAKKCLHVV
                     GPNLNAGEDIQLLKAAYENFNSQDILLAPLLSAGIFGAKPLQSLQVCVQTVRTQVYIAV
                     NDKALYEQVVMDYLDNLKPRVEAPKQEEPPNTEDSKTEEKSVVQKPVDVKPKIKACIDE
                     VTTTLEETKFLTNKLLLFADINGKLYHDSQNMLRGEDMSFLEKDAPYMVGDVITSGDIT
                     CVVIPSKKAGGTTEMLSRALKKVPVDEYITTYPGQGCAGYTLEEAKTALKKCKSAFYVL
                     PSEAPNAKEEILGTVSWNLREMLAHAEETRKLMPICMDVRAIMATIQRKYKGIKIQEGI
                     VDYGVRFFFYTSKEPVASIITKLNSLNEPLVTMPIGYVTHGFNLEEAARCMRSLKAPAV
                     VSVSSPDAVTTYNGYLTSSSKTSEEHFVETVSLAGSYRDWSYSGQRTELGVEFLKRGDK
                     IVYHTLESPVEFHLDGEVLSLDKLKSLLSLREVKTIKVFTTVDNTNLHTQLVDMSMTYG
                     QQFGPTYLDGADVTKIKPHVNHEGKTFFVLPSDDTLRSEAFEYYHTLDESFLGRYMSAL
                     NHTKKWKFPQVGGLTSIKWADNNCYLSSVLLALQQLEVKFNAPALQEAYYRARAGDAAN
                     FCALILAYSNKTVGELGDVRETMTHLLQHANLESAKRVLNVVCKHCGQKTTTLTGVEAV
                     MYMGTLSYDNLKTGVSIPCVCGRDATQYLVQQESSFVMMSAPPAEYKLQQGTFLCANEY
                     TGNYQCGHYTHITAKETLYRIDGAHLTKMSEYKGPVTDVFYKETSYTTTIKPVSYKLDG
                     VTYTEIEPKLDGYYKKDNAYYTEQPIDLVPTQPLPNASFDNFKLTCSNTKFADDLNQMT
                     GFTKPASRELSVTFFPDLNGDVVAIDYRHYSASFKKGAKLLHKPIVWHINQATTKTTFK
                     PNTWCLRCLWSTKPVDTSNSFEVLAVEDTQGMDNLACESQQPTSEEVVENPTIQKEVIE
                     CDVKTTEVVGNVILKPSDEGVKVTQELGHEDLMAAYVENTSITIKKPNELSLALGLKTI
                     ATHGIAAINSVPWSKILAYVKPFLGQAAITTSNCAKRLAQRVFNNYMPYVFTLLFQLCT
                     FTKSTNSRIRASLPTTIAKNSVKSVAKLCLDAGINYVKSPKFSKLFTIAMWLLLLSICL
                     GSLICVTAAFGVLLSNFGAPSYCNGVRELYLNSSNVTTMDFCEGSFPCSICLSGLDSLD
                     SYPALETIQVTISSYKLDLTILGLAAEWVLAYMLFTKFFYLLGLSAIMQVFFGYFASHF
                     ISNSWLMWFIISIVQMAPVSAMVRMYIFFASFYYIWKSYVHIMDGCTSSTCMMCYKRNR
                     ATRVECTTIVNGMKRSFYVYANGGRGFCKTHNWNCLNCDTFCTGSTFISDEVARDLSLQ
                     FKRPINPTDQSSYIVDSVAVKNGALHLYFDKAGQKTYERHPLSHFVNLDNLRANNTKGS
                     LPINVIVFDGKSKCDESASKSASVYYSQLMCQPILLLDQALVSDVGDSTEVSVKMFDAY
                     VDTFSATFSVPMEKLKALVATAHSELAKGVALDGVLSTFVSAARQGVVDTDVDTKDVIE
                     CLKLSHHSDLEVTGDSCNNFMLTYNKVENMTPRDLGACIDCNARHINAQVAKSHNVSLI
                     WNVKDYMSLSEQLRKQIRSAAKKNNIPFRLTCATTRQVVNVITTKISLKGGGKPIPNPL
                     LGLDST"
     regulatory      666..675
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6441..6482
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          6512..7084
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      7281..7336
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(7673..7869)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7687..7822
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(8107..8695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8866..9639)
                     /codon_start=1
                     /label=AMP
                     /translation="MLKISWVHEWVTSNWISTAVRSLRVFAPKNVFERFPMMSTFKVLL
                     CGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAA
                     NLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLL
                     TGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDG
                     KPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             complement(9164..9542)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(9737..9841)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10173..10628)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     10773..10789
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10796..10814
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catggccccc attaagggcg tgaccttcgg cgaggacacc gtgtgggagg
      721 tgcagggcta taagaatgtg agaatcacat ttgagctgga tgagagagtg gataaagtgc
      781 tgaatgagaa atgtagcgtg tacaccgtgg agtctggaac agaggtgaca gagttcgcct
      841 gtgtggtggc tgaggccgtg gttaagacac tgcagccagt gtctgacctg ctgacaaaca
      901 tgggcatcga tctggatgag tggagtgtgg ctacatttta cctgtttgat gacgctggcg
      961 aggagaattt tagctctagg atgtactgta gcttctaccc ccctgatgaa gaagaagaag
     1021 atgatgctga gtgtgaagaa gaggagatcg atgagacctg cgagcacgag tacggcaccg
     1081 aggacgacta ccagggcctg cccctggagt tcggcgccag cgccgagacc gtgagggtgg
     1141 aggaagagga ggaggaggac tggctggacg acaccaccga gcagtccgag atagagcccg
     1201 agcccgagcc aacgcctgag gagccagtga accagttcac cggctacctg aagctgaccg
     1261 acaacgtggc cattaagtgc gtggacatag tgaaggaggc ccagagcgcc aaccctatgg
     1321 tcatcgtgaa tgccgccaac atccacctga agcacggcgg cggcgtggcc ggcgccctga
     1381 acaaggccac caacggcgct atgcagaagg agagcgacga ctacatcaag ctgaatggcc
     1441 ccctgaccgt gggcggcagc tgcctgctga gcggccacaa cctggccaag aagtgcctgc
     1501 acgtggtggg ccccaacctg aacgccggcg aggacatcca gctgctgaag gccgcctacg
     1561 agaacttcaa ctcccaggac atcctgctgg cccccctgct gtccgccggc atctttgggg
     1621 ccaagcccct gcagagcctg caggtgtgcg tgcagaccgt gagaacccag gtgtacatcg
     1681 ccgtgaatga caaggccctg tacgagcagg tggtcatgga ctacctggac aacctgaagc
     1741 ctagggtgga ggcccccaag caggaggagc cccccaacac cgaggacagc aagaccgagg
     1801 agaagtccgt ggtgcagaag cccgtggacg tgaagcccaa aatcaaggcc tgtattgacg
     1861 aggtgaccac caccctggag gagaccaagt tcctgaccaa taagctgctg ctgttcgccg
     1921 acatcaacgg caagctgtac cacgatagcc agaacatgct gaggggcgag gacatgagct
     1981 tcctggagaa agacgccccc tacatggtgg gcgacgtgat caccagcggc gacatcacct
     2041 gcgtcgtgat ccctagcaag aaggccggag gaacaacaga aatgcttagt agagcactga
     2101 agaaggtgcc tgtggatgag tatattacaa catatccagg tcagggatgt gccggataca
     2161 cactggagga agctaagacc gccctgaaaa agtgtaaatc cgccttttac gtgctgccat
     2221 ctgaggcgcc taacgctaaa gaagagatcc tgggaaccgt gtcctggaac ctgagagaga
     2281 tgctggccca cgccgaggag acccggaagc tgatgcccat ctgcatggac gtgcgggcca
     2341 tcatggccac catccagaga aagtacaagg gcattaagat ccaggagggc atcgtggact
     2401 acggcgtgag atttttcttc tacacctcca aggagcccgt ggcctccatc atcaccaagc
     2461 tgaatagcct gaacgagccc ctggtgacca tgcccatcgg ctacgtgacc cacggcttca
     2521 acctggagga ggccgccaga tgcatgagaa gcctgaaggc ccctgccgtg gtgagcgtga
     2581 gcagccccga cgccgtgacc acctacaacg gatacctaac ctccagcagc aagacctccg
     2641 aggagcactt cgtggagacc gtgagcctgg ccggcagcta cagggactgg agctactccg
     2701 gccagaggac cgagctgggc gtggagtttc tgaagagggg cgataagatc gtgtaccata
     2761 ccctggagag ccccgtggag ttccacctgg acggcgaggt gctgagcctg gacaagctga
     2821 agtccctgct gagcctgcgc gaggtcaaga ccatcaaggt gtttacaact gtggataata
     2881 ccaatctgca cacacagctg gtggatatgt ctatgactta tggccagcag ttcggaccaa
     2941 catatctgga tggcgcggat gtgactaaga taaaaccaca cgtgaatcat gaaggaaaga
     3001 cctttttcgt gctgccctcc gatgataccc tgaggtccga agcctttgag tactaccaca
     3061 ccctggacga gagcttcctg ggaaggtaca tgagcgccct gaaccacacc aagaagtgga
     3121 agttccccca ggtggggggc ctgaccagca ttaagtgggc cgacaacaac tgctacctga
     3181 gcagcgtgct gctggccctg caacagctgg aggtgaagtt caacgccccc gccctgcagg
     3241 aggcctacta ccgggccaga gccggcgatg ctgccaattt ctgtgccctg atcctggcct
     3301 actctaacaa gaccgttggc gagctgggcg acgtgaggga gaccatgacc catctgctgc
     3361 agcatgctaa tctggagtct gccaagagag tgctgaatgt ggtgtgcaag cattgtggcc
     3421 agaaaaccac caccctgacc ggcgtggagg ccgtgatgta catgggcacc ctgagctatg
     3481 ataatctcaa aaccggagtg agtattccct gcgtgtgtgg cagggatgcc acacagtacc
     3541 tggtgcagca ggagagcagc tttgtgatga tgagcgcccc tcctgccgag tataaactgc
     3601 agcagggcac atttctgtgc gccaatgaat acaccggtaa ctaccagtgc ggacactaca
     3661 cacacatcac cgccaaggag accctgtata gaattgatgg cgctcacctg acaaagatgt
     3721 ctgagtataa gggcccagtg actgatgtgt tttataaaga gacttcctac acaacaacaa
     3781 ttaagccagt gtcctataag ctggatggag tgacctacac cgaaattgag ccaaaactgg
     3841 atggctatta caaaaaggat aacgcctatt atacagagca acccattgat ctggtgccca
     3901 cccagccact gcctaacgct tcttttgata acttcaagtt aacctgtagt aatacaaaat
     3961 ttgccgacga cctgaaccag atgaccggct ttaccaagcc cgctagtaga gagctgagcg
     4021 tgaccttctt ccctgatctg aacggcgatg tggtggcaat cgattacaga cattatagcg
     4081 cctcctttaa gaaaggcgct aaactgctgc acaagcctat cgtgtggcac attaatcagg
     4141 ccaccaccaa aacaaccttc aaaccaaata catggtgcct gaggtgcctg tggtctacca
     4201 agcccgtgga taccagcaac tcctttgaag tgctggctgt ggaggacaca cagggcatgg
     4261 acaacctggc ctgcgagagc cagcagccta cctccgagga ggtggtggag aaccccacca
     4321 tccagaagga ggtaatcgag tgcgacgtga aaaccaccga ggtggtgggc aacgtgatcc
     4381 tgaagccctc cgacgagggc gtgaaggtga cccaggagct gggccacgag gacctgatgg
     4441 ccgcttacgt ggagaacacc agcatcacta tcaagaagcc taacgagctg tccctggctc
     4501 tggggctcaa gaccatcgcc acacacggca ttgccgccat caacagcgtg ccctggagca
     4561 agatcctggc ctacgtgaag cccttcctgg gccaggccgc catcaccacc agcaattgcg
     4621 ccaagcgcct ggcccagagg gtgttcaata actacatgcc ctacgtgttt accctgctgt
     4681 tccagctgtg taccttcacc aagtccacca atagccgcat cagagccagc ctgcccacca
     4741 ccatcgccaa gaactcggtg aagtcagtgg ccaagctgtg cctggacgcc ggcatcaatt
     4801 acgtgaaaag ccctaagttc tccaagctgt tcaccatcgc catgtggctg ctgctgctgt
     4861 ccatctgcct gggcagcctg atctgcgtga ccgccgcctt cggcgtgctg ctgagcaact
     4921 tcggcgcccc tagctactgt aatggcgtgc gggagctgta cctgaacagc agcaacgtga
     4981 ccacaatgga tttctgcgag ggctccttcc cctgctccat ctgtctgagc ggcctggaca
     5041 gcctggacag ctaccccgcc ctggagacca tccaggtgac catctccagc tacaagctgg
     5101 atctgaccat cctgggcctg gccgccgagt gggtgctggc ttatatgctg ttcacaaagt
     5161 tcttctacct gctgggcctg agcgccatca tgcaggtgtt cttcggctac ttcgccagcc
     5221 acttcatcag caacagctgg ctgatgtggt tcatcatcag catcgtgcag atggcccccg
     5281 tgagcgctat ggtgagaatg tacatcttct tcgccagctt ctactacatc tggaagtcct
     5341 acgtgcacat catggatggc tgcaccagca gcacctgcat gatgtgctac aagaggaatc
     5401 gcgccaccag agtggagtgc accaccatcg tgaacggcat gaagcggagc ttttacgtgt
     5461 acgctaacgg cggccggggc ttctgcaaga cccacaactg gaactgcctg aactgcgaca
     5521 ccttctgcac cggctccacc ttcatctctg acgaggtggc ccgcgacctg agcctgcagt
     5581 tcaagaggcc catcaacccc accgaccaga gcagctacat cgtggactcc gtggccgtga
     5641 agaacggagc cctgcacctg tacttcgaca aggccggcca gaaaacctac gagcggcacc
     5701 ccctgagcca cttcgtgaac ctggacaacc tgagagccaa caataccaag ggcagcctgc
     5761 ccatcaacgt gatcgtgttc gacggcaagt ccaagtgcga tgagtccgcc tccaagagcg
     5821 ccagcgtgta ctactcccag ctgatgtgcc agcctatcct gctgctggac caggccctgg
     5881 tgtccgacgt gggagatagc accgaagtgt ctgtgaagat gtttgacgct tacgtggaca
     5941 ccttctctgc tacattcagc gtgcctatgg agaaactgaa ggccctcgtc gccaccgccc
     6001 acagcgagct ggccaaggga gtggctctgg acggcgtact gtccacattt gtgagcgccg
     6061 ccaggcaggg cgtggtggat accgacgttg ataccaaaga tgtgattgag tgtctgaagc
     6121 tgagccatca tagcgatctg gaggtgaccg gagactcttg caacaacttt atgctgactt
     6181 ataataaggt ggagaatatg acaccaaggg acctgggcgc ctgcatcgac tgtaatgcca
     6241 gacacatcaa tgcccaggtt gccaagtcac ataacgtgtc cctgatttgg aatgtgaagg
     6301 attacatgag cctctctgaa cagctgagaa agcagattag aagcgccgcc aagaagaata
     6361 acattccctt tagactgacc tgcgccacca ccagacaggt ggtgaatgtg atcacaacca
     6421 agattagcct gaagggaggc ggcaaaccca ttcctaatcc tctgctggga ctggatagca
     6481 cctgaacgcg tcggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt
     6541 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt
     6601 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca
     6661 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt
     6721 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc
     6781 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg
     6841 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
     6901 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt
     6961 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
     7021 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct
     7081 acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcac
     7141 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     7201 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     7261 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     7321 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     7381 tgagtatttg gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt
     7441 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     7501 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     7561 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     7621 tactcccagt catagctgtc cctcttctct tatgaagatc ctcgagaagc tgtttgcaaa
     7681 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     7741 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     7801 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     7861 tgagatgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7921 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8281 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8521 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8761 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8821 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8881 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8941 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9001 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9061 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9121 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9181 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9241 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9301 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9361 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9421 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9481 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9541 ttcgaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9601 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9661 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9721 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9781 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9841 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     9901 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     9961 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    10021 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
    10081 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    10141 acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa
    10201 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
    10261 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
    10321 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
    10381 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
    10441 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
    10501 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
    10561 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
    10621 gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    10681 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    10741 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
    10801 cgactcacta tagggcgaat tcgagctcgc ccc
//
