LOCUS       Exported                5292 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             672..944
                     /codon_start=1
                     /label=E
                     /translation="MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCN
                     IVNVSLVKPTVYVYSRVKNLNSSEGVPDLLVGKPIPNPLLGLDST"
     CDS             900..941
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          971..1543
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      1740..1795
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(2132..2328)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2146..2281
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2566..3154)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3623..4001)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(4196..4300)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4632..5087)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5232..5248
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5255..5273
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catgtatagc tttgtgagcg aggagacagg aacactgatt gtgaattctg
      721 tgctgctgtt tctggccttc gtggtgtttc tgctggtgac actggccatt ctgacagccc
      781 tgagactgtg tgcctattgc tgtaatatcg tgaatgtgtc tctggtgaaa cctaccgtgt
      841 acgtgtacag cagagtgaag aatctgaata gctctgaggg cgtgcctgat ctgctggtgg
      901 gcaagcctat ccctaatcct ctgctgggcc tggattctac atgaacgcgt cggatcctga
      961 gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt
     1021 catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt
     1081 gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc
     1141 attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca
     1201 tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta
     1261 atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac agttttagag
     1321 aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg
     1381 tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt
     1441 atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg
     1501 cccctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     1561 ggttgttgtg ctgtctcatc attttggcaa agaattcact cctcaggtgc aggctgccta
     1621 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
     1681 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     1741 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     1801 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     1861 ggcaacatat gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt
     1921 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     1981 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     2041 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     2101 ctcttctctt atgaagatcc tcgagaagct gtttgcaaaa gcctaggcct ccaaaaaagc
     2161 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     2221 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     2281 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcagc tgcattaatg
     2341 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2401 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2461 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2521 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2581 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2641 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2701 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2761 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2821 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2881 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2941 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3001 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3061 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3121 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3181 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3241 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3301 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3361 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3421 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3481 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3541 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3601 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3661 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3721 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3781 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3841 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3901 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3961 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcgaaaacgt tcttcggggc
     4021 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4081 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4141 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4201 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4261 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4321 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     4381 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     4441 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     4501 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     4561 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     4621 accgcatcag gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     4681 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4741 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4801 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4861 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4921 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4981 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     5041 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcgc gccattcgcc
     5101 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     5161 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     5221 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt
     5281 cgagctcgcc cc
//
