LOCUS       Exported                5727 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     regulatory      666..671
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             672..1379
                     /codon_start=1
                     /label=SARS-CoV1 M
                     /translation="MADNGTITVEELKQLLEQWNLVIGFLFLAWIMLLQFAYSNRNRFL
                     YIIKLVFLWLLWPVTLACFVLAAVYRINWVTGGIAIAMACIVGLMWLSYFVASFRLFAR
                     TRSMWSFNPETNILLNVPLRGTIVTRPLMESELVIGAVIIRGHLRMAGHSLGRCDIKDL
                     PKEITVATSRTLSYYKLGASQRVGTDSGFAAYNRYRIGNYKLNTDHAGSNDNIALLVQG
                     KPIPNPLLGLDST"
     CDS             1335..1376
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          1406..1978
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      2175..2230
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(2567..2763)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2581..2716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(3001..3589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4058..4436)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(4631..4735)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5067..5522)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5667..5683
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5690..5708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catggccgat aatggcacca tcaccgtgga ggagctgaag cagctgctgg
      721 agcagtggaa tctcgtgatc ggctttctgt tcctggcctg gattatgctg ctgcagtttg
      781 cctatagcaa tagaaataga ttcctttaca tcatcaagct ggtgtttctg tggctgctgt
      841 ggccagtgac actggcttgt ttcgtgctgg ctgccgtgta tagaatcaat tgggtgactg
      901 gaggcattgc tatcgccatg gcttgtattg tgggcctgat gtggctgagc tactttgtgg
      961 ccagctttag actgtttgct aggacaagat ctatgtggag ctttaatcct gaaaccaaca
     1021 tcctgctgaa tgtgcctctg agaggaacca ttgtgacaag gcctctgatg gagtctgaac
     1081 tcgtgattgg cgccgtgatt atcaggggtc acctgcggat ggccggccac agcctgggca
     1141 ggtgcgacat caaggacctg cccaaggaga tcaccgtggc caccagcaga accctgagct
     1201 actacaagct gggcgccagc cagagagtgg gcaccgacag cggcttcgcc gcctacaaca
     1261 gatacagaat cggcaactac aagctgaaca ccgaccacgc cggaagcaac gacaacatcg
     1321 ccctgctggt gcagggcaag ccgatcccca accccctgct gggcctggac agcacctgaa
     1381 cgcgtcggat cctgagaact tcagggtgag tttggggacc cttgattgtt ctttcttttt
     1441 cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt tgtttagaat
     1501 gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct ttcactttct
     1561 actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa ctttttcgtt
     1621 aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt tgtcagattg
     1681 taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat attgtacttc
     1741 agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat ttctgcatat
     1801 aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc tggtcatcat
     1861 cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga taaaatactc
     1921 tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1981 cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat tcactcctca
     2041 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     2101 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     2161 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     2221 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     2281 tttggtttag agtttggcaa catatgccat atgctggctg ccatgaacaa aggtggctat
     2341 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     2401 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     2461 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     2521 cagtcatagc tgtccctctt ctcttatgaa gatcctcgag aagctgtttg caaaagccta
     2581 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
     2641 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     2701 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     2761 gcagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     3481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcgaa
     4441 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4501 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4561 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4621 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4681 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4741 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     4801 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     4861 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     4921 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     4981 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     5041 gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt taaaattcgc
     5101 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     5161 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     5221 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     5281 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     5341 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     5401 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     5461 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     5521 gtcgcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     5581 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     5641 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
     5701 actatagggc gaattcgagc tcgcccc
//
