LOCUS       Exported                6330 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             672..1982
                     /codon_start=1
                     /label=SARS CoV1 N
                     /translation="MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQG
                     LPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKE
                     LSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLP
                     QGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALAL
                     LLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPE
                     QTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKL
                     DDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAA
                     DMDDFSRQLQNSMSGASADSTQAGKPIPNPLLGLDST"
     CDS             1938..1979
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          2009..2581
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      2778..2833
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(3170..3366)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3184..3319
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(3604..4192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4661..5039)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(5234..5338)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5670..6125)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     6270..6286
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6293..6311
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catgagcgat aatggaccac aatctaatca aagatccgct ccccgcatta
      721 ccttcggtgg gcccacagat agtacggaca ataatcaaaa cggcgggcga aacggggcgc
      781 gacctaagca gcgacgccct caagggctgc ccaacaacac cgcgagctgg tttaccgctc
      841 tgactcaaca tgggaaagaa gagctccggt ttccaagggg acaaggagtt cctataaata
      901 ctaacagcgg tccagacgat cagataggct actaccggag agccacacgt cgtgtcaggg
      961 gtggtgacgg caaaatgaaa gaattgtccc cacggtggta cttttattac ctcggaaccg
     1021 ggcctgaagc aagcctgccc tatggcgcta ataaagaggg aatcgtctgg gtagccaccg
     1081 agggcgctct taatacacct aaggatcaca ttgggacacg aaatcccaat aacaacgctg
     1141 caactgtctt gcaactgccc caagggacca ctctgccgaa ggggttttat gctgaaggta
     1201 gcagaggtgg atctcaagcg tcttctcgct cctcatccag atctcggggt aattccagaa
     1261 actccactcc cggaagctca cggggaaaca gtccagcccg gatggcatca ggcggcggag
     1321 aaaccgccct ggctctgctg ctgctcgaca ggcttaatca acttgaaagc aaggtgtctg
     1381 ggaaagggca acagcagcag ggtcaaacag tgacaaagaa atctgctgct gaggcgagta
     1441 agaagccccg gcagaaacgg acagcaacaa aacaatacaa cgtaactcaa gccttcggac
     1501 gccgcggccc agagcagaca caggggaact ttggcgatca agatttgatc cgccagggaa
     1561 ctgattacaa acattggcct caaattgcgc agttcgctcc tagcgcatca gcgttcttcg
     1621 gcatgagtag aataggcatg gaagtcactc caagcggtac ttggctgacc taccatggcg
     1681 ctatcaaact cgatgacaaa gatccgcaat ttaaagataa tgtcatcctt cttaacaaac
     1741 acatagatgc gtacaaaacc tttccaccta cagaaccgaa gaaggataag aagaagaaga
     1801 ctgatgaagc acaacctctg ccacaacggc aaaagaagca gcccactgtg accctgcttc
     1861 ccgctgcgga catggacgac ttttctcgtc agttgcaaaa cagtatgagc ggtgcgtcag
     1921 ctgattctac acaggcaggt aaaccgatcc ctaaccccct gctcggcctt gattccactt
     1981 gaacgcgtcg gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt
     2041 tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag
     2101 aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt
     2161 tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc
     2221 gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga
     2281 ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac
     2341 ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca
     2401 tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca tcctggtcat
     2461 catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata
     2521 ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct ttttcctaca
     2581 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcactcc
     2641 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     2701 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     2761 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     2821 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     2881 gtatttggtt tagagtttgg caacatatgc catatgctgg ctgccatgaa caaaggtggc
     2941 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     3001 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     3061 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     3121 tcccagtcat agctgtccct cttctcttat gaagatcctc gagaagctgt ttgcaaaagc
     3181 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     3241 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     3301 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     3361 gatgcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3421 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3481 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3541 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3601 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3661 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3721 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3781 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3841 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3901 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3961 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4021 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4081 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4141 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4201 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4261 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4321 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4381 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4441 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4501 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4561 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4621 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4681 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4741 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4801 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4861 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4921 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4981 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5041 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5101 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5161 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5221 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5281 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5341 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5401 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     5461 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     5521 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     5581 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     5641 gatgcgtaag gagaaaatac cgcatcagga aattgtaaac gttaatattt tgttaaaatt
     5701 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     5761 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     5821 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     5881 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     5941 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     6001 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     6061 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     6121 cgcgtcgcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     6181 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     6241 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     6301 ctcactatag ggcgaattcg agctcgcccc
//
