LOCUS       Exported                5253 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             672..905
                     /codon_start=1
                     /label=SARS CoV1 ORF6
                     /translation="MFHLVDFQVTIAEILIIIMRTFRIAIWNLDVIISSIVRQLFKPLT
                     KKNYSELDDEEPMELDYPGKPIPNPLLGLDST"
     CDS             861..902
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          932..1504
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      1701..1756
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(2093..2289)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2107..2242
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2527..3115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3584..3962)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(4157..4261)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4593..5048)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5193..5209
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5216..5234
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catgttccac ttggtggatt ttcaagtgac catcgctgaa atccttatca
      721 ttatcatgcg cacatttaga atagcaatct ggaacttgga tgtgatcatt agctctattg
      781 taaggcagtt gttcaaacca ttgacaaaga agaactactc cgaactggac gacgaggagc
      841 ccatggaact ggactacccc ggcaaaccca ttccaaatcc ccttcttgga ctggacagta
      901 cttaaacgcg tcggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt
      961 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt
     1021 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca
     1081 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt
     1141 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc
     1201 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg
     1261 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
     1321 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt
     1381 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
     1441 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct
     1501 acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcac
     1561 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     1621 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     1681 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     1741 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     1801 tgagtatttg gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt
     1861 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     1921 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     1981 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     2041 tactcccagt catagctgtc cctcttctct tatgaagatc ctcgagaagc tgtttgcaaa
     2101 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     2161 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     2221 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     2281 tgagatgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2341 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2401 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2461 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2521 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2581 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2641 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2701 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2761 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2821 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2881 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2941 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     3001 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3061 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3121 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3181 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3241 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3301 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3361 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3421 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3481 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3541 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3601 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3661 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3721 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3781 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3841 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3901 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3961 ttcgaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4021 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4081 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4141 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4201 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4261 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     4321 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     4381 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     4441 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     4501 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     4561 acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa
     4621 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4681 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4741 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4801 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4861 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4921 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4981 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     5041 gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     5101 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     5161 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
     5221 cgactcacta tagggcgaat tcgagctcgc ccc
//
