LOCUS       Exported                5430 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             672..1082
                     /codon_start=1
                     /label=SARS CoV1 ORF7a
                     /translation="MKIILFLTLIVFTSCELYHYQECVRGTTVLLKEPCPSGTYEGNSP
                     FHPLADNKFALTCTSTHFAFACADGTRHTYQLRARSVSPKLFIRQEEVQQELYSPLFLI
                     VAALVFLILCFTIKRKTEGKPIPNPLLGLDST"
     CDS             1038..1079
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          1109..1681
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      1878..1933
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(2270..2466)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2284..2419
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2704..3292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3761..4139)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(4334..4438)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4770..5225)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5370..5386
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5393..5411
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catgaaaatt attctcttcc tgaccttgat cgtcttcacg tcctgcgaac
      721 tgtaccatta ccaagaatgc gtgaggggca ccacagtcct gctcaaggag ccttgtccga
      781 gtggtactta tgaagggaat agccccttcc atccactggc cgataataaa tttgctctta
      841 cgtgcacgtc cacgcacttt gcctttgcat gcgcagacgg aacgagacat acctaccaat
      901 tgcgagcgag gagtgtctca cccaaactgt ttatcaggca agaagaggta caacaagagt
      961 tgtatagccc cttgtttctg atcgttgcgg ctcttgtatt tcttatactg tgctttacca
     1021 taaagaggaa aacagagggc aaaccaattc ccaatcccct cttgggtctg gactccacat
     1081 agacgcgtcg gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt
     1141 tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag
     1201 aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt
     1261 tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc
     1321 gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga
     1381 ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac
     1441 ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca
     1501 tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca tcctggtcat
     1561 catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata
     1621 ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcactcc
     1741 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     1801 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     1861 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     1921 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     1981 gtatttggtt tagagtttgg caacatatgc catatgctgg ctgccatgaa caaaggtggc
     2041 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     2101 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     2161 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     2221 tcccagtcat agctgtccct cttctcttat gaagatcctc gagaagctgt ttgcaaaagc
     2281 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     2341 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     2401 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     2461 gatgcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2521 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2581 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2641 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2701 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2761 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2821 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2881 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2941 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3001 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3061 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3121 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3301 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3361 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3421 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3481 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3541 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3601 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3661 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3721 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3781 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3841 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3901 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3961 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4021 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4081 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4141 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4201 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4261 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4321 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4381 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4441 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     4501 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     4561 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     4621 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     4681 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     4741 gatgcgtaag gagaaaatac cgcatcagga aattgtaaac gttaatattt tgttaaaatt
     4801 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     4861 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     4921 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     4981 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     5041 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     5101 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     5161 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     5221 cgcgtcgcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     5281 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     5341 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
     5401 ctcactatag ggcgaattcg agctcgcccc
//
