LOCUS       Exported                5604 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     regulatory      666..675
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             672..1256
                     /codon_start=1
                     /label=SARS CoV2 NSP1
                     /translation="MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQH
                     LKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETL
                     GVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNT
                     KHSSGVTRELMRELNGGGKPIPNPLLGLDST"
     CDS             1212..1253
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          1283..1855
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      2052..2107
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(2444..2640)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2458..2593
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2878..3466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3935..4313)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(4508..4612)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4944..5399)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5544..5560
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5567..5585
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catggagagt ctggtccctg gatttaatga gaaaactcac gtgcaactca
      721 gcttgccagt cctgcaggtg cgcgatgtac ttgtaagggg cttcggggat agcgtcgaag
      781 aggttctttc cgaggcacgc caacacctga aagatggtac atgtgggttg gtggaagtcg
      841 agaaaggcgt actcccacaa ttggagcaac catacgtgtt catcaagcgg agcgatgcgc
      901 ggactgcccc acacgggcat gtgatggttg aacttgtggc tgaacttgaa ggcattcaat
      961 atggtaggag cggtgaaacc cttggcgttc tggtgccaca tgtcggcgaa attcccgtgg
     1021 catacaggaa ggtcctgctg cgcaagaatg gaaacaaagg cgctggtggc cattcttacg
     1081 gggcagatct caagagtttc gacttgggcg acgaactggg gacggatcct tacgaagatt
     1141 tccaagaaaa ctggaacaca aaacattctt ccggggtcac ccgcgaactc atgcgcgagc
     1201 tgaatggcgg cgggaagcca ataccaaatc ctctgctggg tttggatagt acttagacgc
     1261 gtcggatcct gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc
     1321 tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg
     1381 aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact
     1441 ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa
     1501 ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa
     1561 gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc
     1621 acagttttag agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa
     1681 ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg tcatcatcct
     1741 gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa aatactctga
     1801 gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1861 gggcaacgtg ctggttgttg tgctgtctca tcattttggc aaagaattca ctcctcaggt
     1921 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     1981 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     2041 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     2101 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     2161 ggtttagagt ttggcaacat atgccatatg ctggctgcca tgaacaaagg tggctataaa
     2221 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     2281 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     2341 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     2401 tcatagctgt ccctcttctc ttatgaagat cctcgagaag ctgtttgcaa aagcctaggc
     2461 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     2521 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
     2581 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
     2641 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2701 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2761 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2821 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2881 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2941 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3001 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3061 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3121 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3181 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3241 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3301 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3361 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3421 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3481 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3541 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3601 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3661 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3721 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3781 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3841 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3901 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3961 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4021 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4081 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4141 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4201 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4261 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcgaaaac
     4321 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4381 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4441 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4501 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4561 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4621 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     4681 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     4741 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     4801 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     4861 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     4921 taaggagaaa ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt
     4981 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     5041 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     5101 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     5161 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     5221 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     5281 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     5341 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     5401 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     5461 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     5521 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     5581 atagggcgaa ttcgagctcg cccc
//
