LOCUS       Exported                5316 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             672..968
                     /codon_start=1
                     /label=NSP7
                     /translation="MSKMSDVKCTSVVLLSVLQQLRVESSSKLWAQCVQLHNDILLAKD
                     TTEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNRATLQGKPIPNPLLGLDST"
     CDS             924..965
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          995..1567
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      1764..1819
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(2156..2352)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2170..2305
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2590..3178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3647..4025)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(4220..4324)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4656..5111)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5256..5272
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5279..5297
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catgagcaaa atgagcgatg ttaaatgtac tagcgttgta ctgctgtccg
      721 tgctccagca attgagagtt gaatcatcaa gtaaactctg ggcacaatgt gttcaattgc
      781 acaacgacat cttgcttgcc aaagatacta ctgaagcgtt cgaaaagatg gtttccttgc
      841 tcagcgtcct tttgtccatg caaggcgcag tcgatatcaa caagctgtgc gaagaaatgc
      901 tggataatcg tgccacgctg caaggcaagc ctatacccaa ccccctgctg ggactggatt
      961 ctacatagac gcgtcggatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
     1021 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
     1081 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
     1141 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
     1201 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
     1261 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
     1321 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
     1381 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
     1441 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
     1501 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
     1561 cctacagctc ctgggcaacg tgctggttgt tgtgctgtct catcattttg gcaaagaatt
     1621 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     1681 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     1741 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     1801 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     1861 gaatgagtat ttggtttaga gtttggcaac atatgccata tgctggctgc catgaacaaa
     1921 ggtggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     1981 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     2041 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     2101 gactactccc agtcatagct gtccctcttc tcttatgaag atcctcgaga agctgtttgc
     2161 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg
     2221 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
     2281 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact
     2341 aattgagatg cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2401 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2521 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2581 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2641 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2701 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2761 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2821 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2881 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2941 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3001 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     3061 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3121 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3181 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3241 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3301 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3361 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3421 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3481 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3541 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3601 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3661 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3721 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3781 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3841 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3901 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3961 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4021 acgttcgaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4081 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4141 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4201 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4261 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4321 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     4381 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     4441 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     4501 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     4561 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     4621 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaaacgtta atattttgtt
     4681 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     4741 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     4801 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     4861 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     4921 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     4981 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     5041 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     5101 acagggcgcg tcgcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     5161 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     5221 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta
     5281 atacgactca ctatagggcg aattcgagct cgcccc
//
