LOCUS       Exported                6105 bp DNA     circular SYN 30-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   CS
  TITLE     Direct Submission
  JOURNAL   Exported Dec 7, 2021 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..303
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     primer_bind     645..681
                     /label=NSP15-SARS2-Fr1-fwd
     CDS             672..1757
                     /codon_start=1
                     /label=NSP15
                     /translation="MSLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFE
                     NKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCS
                     MTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASL
                     NGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEF
                     IERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFI
                     TDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVE
                     TFYPKLQGKPIPNPLLGLDST"
     primer_bind     complement(848..874)
                     /label=NSP15-SARS2-Fr1-rev
     primer_bind     867..881
                     /label=NSP15-SARS2-Fr2-fwd
     primer_bind     complement(1224..1268)
                     /label=NSP-SARS2-Fr2-rev
     primer_bind     1233..1268
                     /label=NSP15-SARS2-Fr3-fwd
     CDS             1713..1754
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(1746..1770)
                     /label=NSP15-SARS2-Fr3-rev
     intron          1784..2356
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_site      2553..2608
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        complement(2945..3141)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2959..3094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(3379..3967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4436..4814)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(5009..5113)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5445..5900)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     6045..6061
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6068..6086
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtaggcctt
      661 ctagagccac catgtcactt gagaacgttg cttttaacgt cgtaaataaa ggacattttg
      721 atggccaaca gggcgaggtt cctgtgtcaa ttattaacaa tactgtctat acaaaggtag
      781 acggagtcga tgttgaactc tttgaaaaca agactactct cccagtgaat gtggcgttcg
      841 aattgtgggc gaagcgtaac attaaaccag tacccgaagt gaaaatcctc aacaatctcg
      901 gtgtggatat tgcagccaat accgtgatct gggattacaa acgcgacgct cctgctcaca
      961 tctctaccat aggggtgtgt tccatgactg acatcgctaa gaaacccacc gagactatat
     1021 gtgcaccact gacagtattc tttgacggaa gagtagatgg tcaagtggac ctgttccgta
     1081 acgctcggaa tggtgtgctg atcactgaag gtagcgtcaa gggactccaa ccgtcagtag
     1141 gcccaaagca agcaagtctg aacggagtga ccctgatagg ggaagccgtc aaaactcaat
     1201 ttaattacta taagaaagtt gacggcgtgg tacaacaact tcctgagaca tatttcaccc
     1261 aaagccgaaa tctgcaagaa tttaaaccac gttctcaaat ggaaattgac ttcctcgaac
     1321 tggccatgga tgagtttatt gagagataca aattggaagg ctatgccttc gagcatatag
     1381 tctacggcga cttttcccat tcacagcttg gcgggctcca tctgcttata gggttggcaa
     1441 agagattcaa agaatcacct ttcgagttgg aagatttcat ccccatggat agcaccgtca
     1501 agaactattt cattaccgac gcccagacgg gaagcagcaa gtgcgtctgc tctgtcatcg
     1561 atctgctcct cgatgacttt gtggagataa ttaaaagcca agacctcagc gtagttagca
     1621 aagtcgtcaa agtgactata gactatactg agatcagctt catgctctgg tgtaaggatg
     1681 gtcacgtcga aaccttctac cccaagctgc aaggaaaacc tattcccaac ccactgctgg
     1741 gtcttgatag cacttagacg cgtcggatcc tgagaacttc agggtgagtt tggggaccct
     1801 tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg gcaaagtttt
     1861 cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct catgataatt
     1921 ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt tcttttcatt
     1981 ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta aattcacttt
     2041 tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag gcaatcaggg
     2101 tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta aatgataagg
     2161 tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg gtagaaacaa
     2221 ctacatcctg gtcatcatcc tgcctttctc tttatggtta caatgatata cactgtttga
     2281 gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg ttcatgcctt
     2341 cttctttttc ctacagctcc tgggcaacgt gctggttgtt gtgctgtctc atcattttgg
     2401 caaagaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     2461 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     2521 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     2581 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     2641 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc
     2701 atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     2761 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     2821 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     2881 tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga tcctcgagaa
     2941 gctgtttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
     3001 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     3061 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     3121 ttgctgacta attgagatgc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3181 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3241 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3301 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3361 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3781 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     3841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4441 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4501 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4561 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4621 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4681 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4741 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4801 cattggaaaa cgttcgaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5101 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     5161 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     5221 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     5281 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     5341 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     5401 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taaacgttaa
     5461 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     5521 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     5581 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     5641 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     5701 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     5761 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     5821 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     5881 tgcgccgcta cagggcgcgt cgcgccattc gccattcagg ctgcgcaact gttgggaagg
     5941 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     6001 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     6061 tgaattgtaa tacgactcac tatagggcga attcgagctc gcccc
//
